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  • 201.
    Abramowicz, Konrad
    et al.
    Umeå University, Faculty of Science and Technology, Department of Mathematics and Mathematical Statistics.
    Arnqvist, Per
    Umeå University, Faculty of Science and Technology, Department of Mathematics and Mathematical Statistics.
    Sjöstedt de Luna, Sara
    Umeå University, Faculty of Science and Technology, Department of Mathematics and Mathematical Statistics.
    Secchi, Piercesare
    Vantini, Simone
    Vitelli, Valeria
    Was it snowing on lake Kassjön in January 4486 BC? Functional data analysis of sediment data.2014In: Proceedings of the Third International Workshop on Functional and Operatorial Statistics (IWFOS 2014), Stresa, Italy, June 2014, 2014Conference paper (Refereed)
  • 202.
    Abramowicz, Konrad
    et al.
    Umeå University, Faculty of Science and Technology, Department of Mathematics and Mathematical Statistics.
    Häger, Charlotte
    Umeå University, Faculty of Medicine, Department of Community Medicine and Rehabilitation, Physiotherapy.
    Hérbert-Losier, Kim
    National Sports Institute of Malaysia.
    Pini, Alessia
    MOX – Department of Mathematics, Politecnico di Milano.
    Schelin, Lina
    Umeå University, Faculty of Social Sciences, Umeå School of Business and Economics (USBE), Statistics. Umeå University, Faculty of Medicine, Department of Community Medicine and Rehabilitation, Physiotherapy.
    Strandberg, Johan
    Umeå University, Faculty of Science and Technology, Department of Mathematics and Mathematical Statistics.
    Vantini, Simone
    MOX – Department of Mathematics, Politecnico di Milano.
    An inferential framework for domain selection in functional anova2014In: Contributions in infinite-dimensional statistics and related topics / [ed] Bongiorno, E.G., Salinelli, E., Goia, A., Vieu, P, Esculapio , 2014Conference paper (Refereed)
    Abstract [en]

    We present a procedure for performing an ANOVA test on functional data, including pairwise group comparisons. in a Scheff´e-like perspective. The test is based on the Interval Testing Procedure, and it selects intervals where the groups significantly differ. The procedure is applied on the 3D kinematic motion of the knee joint collected during a functional task (one leg hop) performed by three groups of individuals.

  • 203.
    Abramowicz, Konrad
    et al.
    Umeå University, Faculty of Science and Technology, Department of Mathematics and Mathematical Statistics.
    Häger, Charlotte
    Umeå University, Faculty of Medicine, Department of Community Medicine and Rehabilitation, Physiotherapy.
    Pini, Alessia
    Umeå University, Faculty of Social Sciences, Umeå School of Business and Economics (USBE), Statistics. Department of Statistical Sciences, Università Cattolica del Sacro Cuore, Milan, Italy.
    Schelin, Lina
    Umeå University, Faculty of Medicine, Department of Community Medicine and Rehabilitation. Umeå University, Faculty of Social Sciences, Umeå School of Business and Economics (USBE), Statistics.
    Sjöstedt de Luna, Sara
    Umeå University, Faculty of Science and Technology, Department of Mathematics and Mathematical Statistics.
    Vantini, Simone
    Nonparametric inference for functional-on-scalar linear models applied to knee kinematic hop data after injury of the anterior cruciate ligament2018In: Scandinavian Journal of Statistics, Vol. 45, no 4, p. 1036-1061Article in journal (Refereed)
    Abstract [en]

    Motivated by the analysis of the dependence of knee movement patterns during functional tasks on subject-specific covariates, we introduce a distribution-free procedure for testing a functional-on-scalar linear model with fixed effects. The procedure does not only test the global hypothesis on the entire domain but also selects the intervals where statistically significant effects are detected. We prove that the proposed tests are provided with an asymptotic control of the intervalwise error rate, that is, the probability of falsely rejecting any interval of true null hypotheses. The procedure is applied to one-leg hop data from a study on anterior cruciate ligament injury. We compare knee kinematics of three groups of individuals (two injured groups with different treatments and one group of healthy controls), taking individual-specific covariates into account.

  • 204.
    Abramowicz, Konrad
    et al.
    Umeå University, Faculty of Science and Technology, Department of Mathematics and Mathematical Statistics.
    Seleznjev, Oleg
    Umeå University, Faculty of Science and Technology, Department of Mathematics and Mathematical Statistics.
    Multivariate piecewise linear interpolation of a random field2011Manuscript (preprint) (Other academic)
    Abstract [en]

    We consider a multivariate piecewise linear interpolation of a continuous random field on a-dimensional cube. The approximation performance is measured by the integrated mean square error. Multivariate piecewise linear interpolator is defined by N field observations on a locations grid (or design). We investigate the class of locally stationary random fields whose local behavior is like a fractional Brownian field in mean square sense and find the asymptotic approximation accuracy for a sequence of designs for large N. Moreover, for certain classes of continuous and continuously differentiable fields we provide the upper bound for the approximation accuracy in the uniform mean square norm.

  • 205.
    Abramowicz, Konrad
    et al.
    Umeå University, Faculty of Science and Technology, Department of Mathematics and Mathematical Statistics.
    Seleznjev, Oleg
    Umeå University, Faculty of Science and Technology, Department of Mathematics and Mathematical Statistics.
    On the error of the Monte Carlo pricing method for Asian option2008In: Journal of Numerical and Applied Mathematics, ISSN 0868-6912, Vol. 96, no 1, p. 1-10Article in journal (Refereed)
    Abstract [en]

    We consider a Monte Carlo method to price a continuous arithmetic Asian option with a given precision. Piecewise constant approximation and plain simulation are used for a wide class of models based on L\'{e}vy processes. We give bounds of the possible discretization and simulation errors. The sufficient numbers of discretization points and simulations to obtain requested accuracy are derived. To demonstrate the general approach, the Black-Scholes model is studied in more detail. We undertake the case of continuous averaging and starting time zero,  but the obtained results can be applied to the discrete case  and generalized for any time before an execution date. Some numerical experiments and comparison to the PDE based method are also presented.

  • 206.
    Abramowicz, Konrad
    et al.
    Umeå University, Faculty of Science and Technology, Department of Mathematics and Mathematical Statistics.
    Seleznjev, Oleg
    Umeå University, Faculty of Science and Technology, Department of Mathematics and Mathematical Statistics.
    Piecewise multilinear interpolation of a random field2013In: Advances in Applied Probability, ISSN 0001-8678, E-ISSN 1475-6064, Vol. 45, no 4, p. 945-959Article in journal (Refereed)
    Abstract [en]

    We consider a piecewise-multilinear interpolation of a continuous random field on a d-dimensional cube. The approximation performance is measured using the integrated mean square error. Piecewise-multilinear interpolator is defined by N-field observations on a locations grid (or design). We investigate the class of locally stationary random fields whose local behavior is like a fractional Brownian field, in the mean square sense, and find the asymptotic approximation accuracy for a sequence of designs for large N. Moreover, for certain classes of continuous and continuously differentiable fields, we provide the upper bound for the approximation accuracy in the uniform mean square norm.

  • 207.
    Abramowicz, Konrad
    et al.
    Umeå University, Faculty of Science and Technology, Department of Mathematics and Mathematical Statistics.
    Seleznjev, Oleg
    Umeå University, Faculty of Science and Technology, Department of Mathematics and Mathematical Statistics.
    Spline approximation of a random process with singularity2011In: Journal of Statistical Planning and Inference, ISSN 0378-3758, E-ISSN 1873-1171, Vol. 141, no 3, p. 1333-1342Article in journal (Refereed)
    Abstract [en]

    Let a continuous random process X defined on [0,1] be (m+β)-smooth, 0m, 0<β1, in quadratic mean for all t>0 and have an isolated singularity point at t=0. In addition, let X be locally like a m-fold integrated β-fractional Brownian motion for all nonsingular points. We consider approximation of X by piecewise Hermite interpolation splines with n free knots (i.e., a sampling design, a mesh). The approximation performance is measured by mean errors (e.g., integrated or maximal quadratic mean errors). We construct a sequence of sampling designs with asymptotic approximation rate n^(m+β) for the whole interval.

  • 208.
    Abramowicz, Konrad
    et al.
    Umeå University, Faculty of Science and Technology, Department of Mathematics and Mathematical Statistics.
    Seleznjev, Oleg
    Umeå University, Faculty of Science and Technology, Department of Mathematics and Mathematical Statistics.
    Stratified Monte Carlo quadrature for continuous random fields2015In: Methodology and Computing in Applied Probability, ISSN 1387-5841, E-ISSN 1573-7713, Vol. 17, no 1, p. 59-72Article in journal (Refereed)
    Abstract [en]

    We consider the problem of numerical approximation of integrals of random fields over a unit hypercube. We use a stratified Monte Carlo quadrature and measure the approximation performance by the mean squared error. The quadrature is defined by a finite number of stratified randomly chosen observations with the partition generated by a rectangular grid (or design). We study the class of locally stationary random fields whose local behavior is like a fractional Brownian field in the mean square sense and find the asymptotic approximation accuracy for a sequence of designs for large number of the observations. For the H¨older class of random functions, we provide an upper bound for the approximation error. Additionally, for a certain class of isotropic random functions with an isolated singularity at the origin, we construct a sequence of designs eliminating the effect of the singularity point.

  • 209.
    Abramowizc, Konrad
    et al.
    Umeå University, Faculty of Science and Technology, Department of Mathematics and Mathematical Statistics.
    Arnqvist, Per
    Umeå University, Faculty of Science and Technology, Department of Mathematics and Mathematical Statistics.
    Secchi, Piercesare
    Politecnico di Milano, Italy.
    Sjöstedt de Luna, Sara
    Umeå University, Faculty of Science and Technology, Department of Mathematics and Mathematical Statistics.
    Vantini, Simone
    Politecnico di Milano, Italy.
    Vitelli, Valeria
    Oslo University, Norway.
    Clustering misaligned dependent curves applied to varved lake sediment for climate reconstruction2017In: Stochastic environmental research and risk assessment (Print), ISSN 1436-3240, E-ISSN 1436-3259, Vol. 31, no 1, p. 71-85Article in journal (Refereed)
    Abstract [en]

    In this paper we introduce a novel functional clustering method, the Bagging Voronoi K-Medoid Aligment (BVKMA) algorithm, which simultaneously clusters and aligns spatially dependent curves. It is a nonparametric statistical method that does not rely on distributional or dependency structure assumptions. The method is motivated by and applied to varved (annually laminated) sediment data from lake Kassjön in northern Sweden, aiming to infer on past environmental and climate changes. The resulting clusters and their time dynamics show great potential for seasonal climate interpretation, in particular for winter climate changes.

  • 210.
    Abramsson, Andreas
    et al.
    Umeå University, Faculty of Science and Technology, Department of Science and Mathematics Education.
    Karlsson, David
    Umeå University, Faculty of Science and Technology, Department of Science and Mathematics Education.
    Pojkar som skriver: En kvalitativ studie om vad som utmärker mer avancerade skrivare2017Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
    Abstract [sv]

    Syftet med denna studie är att öka kunskapen om vad som utmärker mer avancerade manliga skrivare på mellanstadiet. Genom en enkätundersökning av pojkars attityder till skrivande samt observationer av pojkars skrivpraktiker före och under ett längre skrivprojekt studerades aspekter som utmärker dessa skrivare gentemot övriga elever. Studien fann att de mer avancerade manliga skrivarna kommer från högutbildade familjer samt att de avancerade skrivarnas positiva attityder till skrivandet var väl förankrade i hemmet. Studien fann också att dessa pojkar i högre uträckning anstränger sig för sin egen, lärarens och sina vårdnadshavares skull än deras klasskamrater. Slutsatsen är att vårdnadshavares värderingar samt att läraren har höga förväntningar på pojkarna avspeglar sig i en vilja att anstränga sig för att lära – vilket i studien visade sig vara nyckeln till att bli en mer avancerad skrivare

  • 211.
    Abramsson, Anna
    et al.
    Umeå University, Faculty of Social Sciences, Department of Informatics.
    Pursiainen, Peter
    Umeå University, Faculty of Social Sciences, Department of Informatics.
    Sandman, Jonas
    Umeå University, Faculty of Social Sciences, Department of Informatics.
    Systemutveckling som designarbete – En studie av en systemutvecklingsprocess 2003Independent thesis Basic level (degree of Bachelor), 15 credits / 22,5 HE creditsStudent thesis
  • 212.
    Abramsson, Anna
    et al.
    Umeå University, Faculty of Social Sciences, Department of Informatics.
    Sandman, Jonas
    Umeå University, Faculty of Social Sciences, Department of Informatics.
    Överlever Internet? En granskning av hoten mot Internets infrastruktur2004Independent thesis Advanced level (degree of Master (One Year)), 10 credits / 15 HE creditsStudent thesis
  • 213.
    Abramsson, Evelina
    et al.
    Umeå University, Faculty of Social Sciences, Umeå School of Business and Economics (USBE), Statistics.
    Grind, Kajsa
    Umeå University, Faculty of Social Sciences, Umeå School of Business and Economics (USBE), Statistics.
    Skattning av kausala effekter med matchat fall-kontroll data2017Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 214.
    Abramsson, Linnea
    Umeå University, Faculty of Medicine, Department of Pharmacology and Clinical Neuroscience, Pharmacology.
    Följsamhet till behandling med bisfosfonater: En intervjustudie på ortopedavdelningen vid Norrlands Universitetssjukhus, Umeå2017Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 215.
    Abramsson, Linnea
    et al.
    Umeå University, Faculty of Medicine, Department of Pharmacology and Clinical Neuroscience.
    Gustafsson, Maria
    Umeå University, Faculty of Medicine, Department of Pharmacology and Clinical Neuroscience, Pharmacology.
    Adherence to Bisphosphonates among People Admitted to an Orthopaedic and Geriatric Ward at a University Hospital in Sweden2018In: Pharmacy, ISSN 2226-4787, E-ISSN 1913-4711, Vol. 6, no 1, article id 20Article in journal (Refereed)
    Abstract [en]

    Oral bisphosphonates are the first choice of therapy to reduce the risk of osteoporotic fractures. These medications have generally poor oral bioavailability, which may further be reduced by concomitant intake of certain foods and drugs; therefore, it is vital to follow specific instructions. The aim with this study was to assess general adherence to oral bisphosphonates and adherence to specific administration instructions among people admitted to two wards at Umeå University hospital in Sweden. This interview study focuses on elderly patients living at home and prescribed oral bisphosphonates. Invited were 27 patients admitted to an orthopaedic ward and a geriatric ward during the period 28 March 2017 and 5 December 2017. In total, 21 patients were interviewed regarding their adherence to oral bisphosphonates. Out of 21 patients, 13 (62%) were considered non-adherent. The most common reason was calcium intake less than 2 h after oral administration of bisphosphonate (54%). The number of regularly prescribed drugs was significantly higher among patients rated non-adherent to bisphosphonates compared to those rated adherent (p = 0.004). Adherence to bisphosphonates administration instruction among elderly people living at home was limited. More research is needed to confirm these results and to investigate the reasons for non-adherence and how adherence to bisphosphonates can be improved.

  • 216.
    Abramsson, Marianne
    Umeå University, Faculty of Social Sciences, Social and Economic Geography.
    Housing Careers in a Changing Welfare State2003Doctoral thesis, monograph (Other academic)
    Abstract [en]

    The departure point of this thesis was changes to the welfare state. These changes began during the recession in the 1970s and were emphasised in the beginning of the 1990s. The housing market was particularly affected, as the state almost completely withdrew its engagement. Questions were raised as to how the development of the welfare state and the subsequent changes have affected different sectors of the housing market (social housing) and the housing careers of different groups.

    Besides the introduction the thesis consists of five papers, beginning with a review article on the effect of welfare state changes on the social housing stock in some western countries. The social housing sector (housing the lowest income groups) became more residualised as the concentration of low-income households in this sector increased in most countries. At the same time, government interest in this sector decreased.

    The second, third and fourth papers follow the housing careers of different groups in three Swedish municipalities during the period 1985–1995. Nest-leaving and choice of tenure of the young population, aged 16–24, in the Gävle municipality were studied. A study of two cohorts showed that nest-leaving was postponed during the recession of the early 1990s when unemployment rates were high among young people. A slight increase in moves to co-operative housing was seen during this period. Immigrants in Jönköping, Gävle and Västerås were studied. Results show that the structure of the local housing market as well as time spent in Sweden are important factors in the housing careers of immigrants. In areas with a large share of owner-occupation, immigrants live in this type of housing to a larger extent. A housing career similar to that of the Swedish population is more common among immigrants who have spent a longer time in Sweden. Residential mobility of seniors towards the city centres in the same municipalities showed only a marginal increase in this type of move. However, the characteristics of those moving were those that, to a large extent, characterise the baby boom generation born in the 1940s. As a result, this type of move can be expected to increase as this group retires.

    Finally, the fifth paper follows the housing career of four cohorts during the development of the welfare state after 1945. The cohorts were born in 1925, 1942, 1955 and 1970, respectively. They were interviewed about the meaning attached to the terms size, tenure and location, important factors in the development of Swedish housing policy. Despite differences in the housing market at the start of their housing careers, with time the cohorts developed similar demands on size, standard and location. Tenure was not cohort-specific but was instead discussed as degrees of freedom found in owner occupation or rental tenure.

  • 217.
    Abramsson, Linnea
    Umeå University, Faculty of Medicine, Department of Pharmacology and Clinical Neuroscience, Pharmacology.
    PREVALENCE OF DRUG RELATED PROBLEMS STOPP/START in elderly people with dementia2019Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
  • 218.
    Abreu, Ilka Nacif
    et al.
    Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC). Umeå University, Faculty of Science and Technology, Department of Plant Physiology.
    Ahnlund, Maria
    Moritz, Thomas
    Albrectsen, Benedicte R.
    Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
    UHPLC-ESI/TOFMS Determination of Salicylate-like Phenolic Gycosides in Populus tremula Leaves2011In: Journal of Chemical Ecology, ISSN 0098-0331, E-ISSN 1573-1561, Vol. 37, no 8, p. 857-70Article in journal (Refereed)
    Abstract [en]

    Associations of salicylate-like phenolic glycosides (PGs) with biological activity have been reported in Salix and Populus trees, but only for a few compounds, and in relation to a limited number of herbivores. By considering the full diversity of PGs, we may improve our ability to recognize genotypes or chemotype groups and enhance our understanding of their ecological function. Here, we present a fast and efficient general method for salicylate determination in leaves of Eurasian aspen that uses ultra-high performance liquid chromatography-electrospray ionization/time-of-flight mass spectrometry (UHPLC-ESI/TOFMS). The time required for the liquid chromatography separations was 13.5 min per sample, compared to around 60 min per sample for most HPLC protocols. In leaf samples from identical P. tremula genotypes with diverse propagation and treatment histories, we identified nine PGs. We found the compound-specific mass chromatograms to be more informative than the UV-visible chromatograms for compound identification and when quantitating samples with large variability in PG content. Signature compounds previously reported for P. tremoloides (tremulacin, tremuloidin, salicin, and salicortin) always were present, and five PGs (2'-O-cinnamoyl-salicortin, 2'-O-acetyl-salicortin, 2'-O-acetyl-salicin, acetyl-tremulacin, and salicyloyl-salicin) were detected for the first time in P. tremula. By using information about the formic acid adduct that appeared for PGs in the LTQ-Orbitrap MS environment, novel compounds like acetyl-tremulacin could be tentatively identified without the use of standards. The novel PGs were consistently either present in genotypes regardless of propagation and damage treatment or were not detectable. In some genotypes, concentrations of 2'-O-acetyl-salicortin and 2'-O-cinnamoyl-salicortin were similar to levels of biologically active PGs in other Salicaceous trees. Our study suggests that we may expect a wide variation in PG content in aspen populations which is of interest both for studies of interactions with herbivores and for mapping population structure.

  • 219.
    ABUDULIMU, ABASI
    Umeå University, Faculty of Science and Technology, Department of Physics.
    Effectof Growth Time, Growth Temperature and Light  on Growth Mechanism of C60 nanorods2013Independent thesis Advanced level (degree of Master (Two Years)), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    In this thesis work C60 nanorods were produced by Liquid-Liquid Interfacial Precipitation method (LLIP) assisted with 10 s of weak sonication. Ethanol and m-dichlorobenzene were used as poor and good solvents of C60, respectively. Five different temperatures, 4, 10, 20, 30, 40 and 50                         , were chosen as growth temperatures of different samples to investigate the effect of temperature on the grown structures. Different samples were prepared in the dark and under the light with various growth time to determine the effect of light and growth time on growth of C60 nanorods. The characterization of the grown C60 nanorods were conducted by transmission electron microscopy (TEM) and x-ray diffraction (XRD). The result of characterization indicated that the sonication introduced smaller C60 nanostructures; light irradiation and temperature increase (till 40 C0) during the growth time resulted in nanorods with smaller diameter, whereas the long growth time lead to the increase of the diameter of C60 nanorods. The as-grown C60 nanorods synthesized at different conditions possess an hcp crystal structure.    

  • 220.
    ABUDULIMU, ABASI
    Umeå University, Faculty of Science and Technology, Department of Physics.
    Measuring the efficiency and charge carrier mobility of organic solar cells2012Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    P3HT single layer, P3HT/PCBM bilayer and P3HT/PCBM inverted bilayer devices were produced by spin coating organic layers onto ITO patterned glass in air, and clamping it with an Au coated silicon wafer, as top electrode, at the end (Figure13). Normal and inverted bilayer devices were also fabricated with and without PEDOT:PSS. All devices were divided into two groups by changing concentration of P3HT solution. The first group of devices contained 1.0 wt. % P3HT solution (P3HT in dichlorobenzene); the second group 0.56wt %. Power conversion efficiency, short circuit current, open circuit voltage, fill factor and maximum extracted power were measured on all produced devices.

    In contrast, all devices with 1.0wt % P3HT concentration showed better result than the devices with 0.56wt %. The highest result was obtained for P3HT single layer devices in both cases with short circuit current 56uA/cm2, open circuit voltage 0.94mV, maximum power 11.4uW/cm2 and power conversion efficiency of 0.11%. Inverted bilayer devices performed better than the non-inverted one. The devices with PEDOT:PSS got slightly better performance than the non-PEDOT:PSS used one.

    Charge carrier mobility measurement was done for all fabricated devices with charge extraction by linearly increasing voltage (CELIV) and dark injected space charge limited current (DI-SCLC) methods. All devices showed same magnitude of charge carrier mobility 10-5 cm2/V.s, the highest value still belongs to P3HT single layer device. The charge carrier mobility in all devices observed by DI-SCLC technique is one order of magnitude higher than by CELIV technique. This may be due to DI-SCLC method`s restriction on ohmic contacts between material and electrode.

  • 221. Abu-Elyazeed, R R
    et al.
    Heineman, T
    Dubin, G
    Fourneau, M
    Leroux-Roels, I
    Leroux-Roels, G
    Richardus, J H
    Ostergaard, L
    Diez-Domingo, J
    Poder, A
    Van Damme, P
    Romanowski, B
    Blatter, M
    Silfverdal, Sven Arne
    Umeå University, Faculty of Medicine, Department of Clinical Sciences, Paediatrics.
    Berglund, J
    Josefsson, A
    Cunningham, A L
    Flodmark, C E
    Tragiannidis, A
    Dobson, S
    Olafsson, J
    Puig-Barbera, J
    Mendez, M
    Barton, S
    Bernstein, D
    Mares, J
    Ratner, P
    Safety and immunogenicity of a glycoprotein D genital herpes vaccine in healthy girls 10-17 years of age: results from a randomised, controlled, double-blind trial2013In: Vaccine, ISSN 0264-410X, E-ISSN 1873-2518, Vol. 31, no 51, p. 6136-6143Article in journal (Refereed)
    Abstract [en]

    OBJECTIVE: The investigational AS04-adjuvanted herpes simplex virus type 2 (HSV-2) glycoprotein D (gD2) subunit prophylactic vaccine ('HSV vaccine'; GlaxoSmithKline Vaccines) has been shown to be well tolerated in adults, but limited data exist for pre-teen and adolescent girls, a likely target population. The primary objective of this study was to compare the occurrence of serious adverse events (SAEs) over 12 months between HSV vaccine recipients and saline recipients (placebo control group) in pre-teen and adolescent girls. The immunogenicity of the HSV vaccine was also assessed.

    METHODS: Healthy girls aged 10-17 years, stratified by age (10-15 years; 16-17 years), were randomised 2:1:1 to receive the HSV vaccine, a hepatitis A vaccine (Havrix™; HAV control) or placebo (saline) according to a 0-, 1-, 6-month schedule. Participants and study personnel not involved in the preparation or administration of vaccines were blinded to treatment. Safety and immunogenicity analyses were performed overall and by age (10-15 years; 16-17 years) and HSV serostatus.

    RESULTS: No statistically significant difference in the percentage of subjects with SAEs was observed between the HSV and saline group, or between the HSV and pooled control (HAV and saline) groups. The HSV vaccine was well tolerated, although a higher incidence of solicited local symptoms was observed in the HSV group than in the control group. Neither age nor HSV serostatus at the time of study entry had an impact on the safety profile of this vaccine. The HSV vaccine was immunogenic regardless of pre-vaccination HSV serostatus. Higher anti-gD geometric mean concentrations were observed in HSV-1 seropositive participants than in HSV-1 seronegative participants.

    CONCLUSION: The HSV vaccine had an acceptable safety profile, and was well tolerated and immunogenic when administered to girls aged 10-17 years regardless of age or HSV pre-vaccination serostatus.

  • 222.
    Abu-Hamam, Anas
    et al.
    Umeå University, Faculty of Science and Technology, Department of Applied Physics and Electronics.
    Li, Haibo
    Umeå University, Faculty of Science and Technology, Department of Applied Physics and Electronics.
    Georgakis, Apostolos
    Umeå University, Faculty of Science and Technology, Department of Applied Physics and Electronics.
    Understanding Web Users Behaviour From A Web Video Camera2003Report (Other academic)
    Abstract [en]

    To develop a Real-Time system, which can capture and track the web user’s head motion. Based on the head motion information, the system should be able to identify whether the user is interested in the current homepage or not. A future system property would be to find out in which area of the homepage the user’s interest is moving.

  • 223. Abul-Kasim, Kasim
    et al.
    Backman, Clas
    Umeå University, Faculty of Medicine, Department of Surgical and Perioperative Sciences, Hand Surgery.
    Björkman, Anders
    Dahlin, Lars B
    Advanced radiological work-up as an adjunct to decision in early reconstructive surgery in brachial plexus injuries2010In: Journal of Brachial Plexus and Peripheral Nerve Injury, ISSN 1749-7221, E-ISSN 1749-7221, Vol. 5, p. 14-Article in journal (Refereed)
    Abstract [en]

    Background

    As neurophysiologic tests may not reveal the extent of brachial plexus injury at the early stage, the role of early radiological work-up has become increasingly important. The aim of the study was to evaluate the concordance between the radiological and clinical findings with the intraoperative findings in adult patients with brachial plexus injuries.

    Methods

    Seven consecutive male patients (median age 33; range 15-61) with brachial plexus injuries, caused by motor cycle accidents in 5/7 patients, who underwent extensive radiological work-up with magnetic resonance imaging (MRI), computed tomography myelography (CT-M) or both were included in this retrospective study. A total of 34 spinal nerve roots were evaluated by neuroradiologists at two different occasions. The degree of agreement between the radiological findings of every individual nerve root and the intraoperative findings was estimated by calculation of kappa coefficient (К-value). Using the operative findings as a gold standard, the accuracy, sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of the clinical findings and the radiological findings were estimated.

    Results

    The diagnostic accuracy of radiological findings was 88% compared with 65% for the clinical findings. The concordance between the radiological findings and the intraoperative findings was substantial (К = 0.76) compared with only fair (К = 0.34) for the clinical findings. There were two false positive and two false negative radiological findings (sensitivity and PPV of 0.90; specificity and NPV of 0.87).

    Conclusions

    The advanced optimized radiological work-up used showed high reliability and substantial agreement with the intraoperative findings in adult patients with brachial plexus injury.

  • 224.
    Abutaleb, Hind
    Umeå University, Faculty of Medicine, Department of Pharmacology and Clinical Neuroscience, Pharmacology.
    Biverkningar avsolskyddsprodukter: Retrospektiv analys av allvarliga biverkningarinrapporterade till Läkemedelsverket underperioden 2013–20172019Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
  • 225.
    Acan, Ali Ramlat
    Umeå University, Faculty of Social Sciences, Umeå School of Business and Economics (USBE), Business Administration.
    Network Governance: The Role of Power and Trust in Mandated Collaboration Network2014Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    Mandated collaboration networks are an overly studied topic in the field of public administration and management, with the emphasis on these studies however focused on the failures to accomplish its collaborative aims. The role that mandated collaboration networks play today in enabling societies and governments alike,  to realize insurmountable challenges through their  collaborative efforts is however not being paid as much attention as it should be, yet through it, huge socio and economic benefits are derived. 

    This study recognizes the part mandated collaboration network plays by seeking to further investigate the role, power and trust play in influencing managers towards attaining efficiency. Data was collected from 7 managers from the public sector, with some public managers, tasked with the responsibility of playing oversight role and disbursing funds and other public managers tasked with implementing the services, all working towards achieving a regional goal within Västerbotten region. By conducting semi-structured interviews with them, the aim was to find out the daily encounters they faced in implementing their activities and achieving their goals.

    In order to analyze this study adequately, theories were derived from governance, principal agency, structuration theory, Long & Sitkin integrated trust and control framework  that enabled me to come up with a conceptual framework. The findings of this framework were particularly insightful in regards to how managers in mandated collaboration network can use trust in ensuring that they achieve their desired efficiency goals. The findings show both power and trust in mandated collaboration network play a coordinative and regulative role in ensuring that the goals are realized. Calculative trust alongside formal controls can be used to address challenges that managers encounter in realizing their goals. Relational trust can also be nurtured, however at an interpersonal level or with peers that perform the same activities but not at an institutional level such as the mandated collaboration network. Attaining efficiency in the mandated collaboration network is however also further compounded by contextual matters both internal and external that hamper its attainment. 

  • 226.
    Acar, Sevil
    et al.
    Istanbul Kemerburgaz University.
    Lindmark, Magnus
    Umeå University, Faculty of Social Sciences, Department of Economic History.
    Convergence of CO2 emissions and economic growth in the OECD countries: did the type of fuel matter?2017In: Energy Sources, Part B: Economics, Planning, and Policy, ISSN 1556-7249, Vol. 12, no 7, p. 618-627Article in journal (Refereed)
    Abstract [en]

    This study analyzes convergence in CO2 emissions in the OECD countries with respect to the source of emissions (oil versus coal). The investigated period 1973-2010 is divided into two sub-periods, 1973-1991 and 1992-2010. The first period covers the OPEC oil price shocks, where the OECD oil policy was to a high extent governed by energy security concerns and cold war strategic considerations. The second period corresponds to the end of the cold war and the rise of climate policy in several OECD countries. Due to such contextual differences, oil and coal behave differently in the two sub-periods. The generally stronger convergence with respect to oil-related emissions until 1991 conditional on GDP per capita is compatible with a situation where the rising oil prices led to a strong transformation in the countries of interest. Besides, we evidence decoupling of economic growth from oil-related emissions in the post-cold war period.

  • 227.
    Acar, Sevil
    et al.
    Istanbul Kemerburgaz University, Turkey.
    Lindmark, Magnus
    Umeå University, Faculty of Social Sciences, Department of Economic History.
    Periods of converging carbon dioxide emissions from oil combustion in a pre-Kyoto context2016In: Environmental Development, ISSN 2211-4645, Vol. 19, p. 1-9Article in journal (Refereed)
    Abstract [en]

    This paper examines convergence of carbon dioxide (CO2) emissions caused by oil combustion for a panel of 86 countries considering the importance of analyzing sub-periods separately. The investigation also points at the necessity of choosing a restricted global sample, which takes into account, for instance, that Eastern Bloc countries reacted differently to increasing world crude oil prices than the rest of the world. The analysis builds on examining the β-convergence hypothesis in a neoclassical growth model setting with additional control variables such as emissions from combustion of solid fuels. The results reveal evidence in support of unconditional β-convergence of CO2 emissions intensity due to oil combustion in the restricted sample for the sub-periods 1973–1979 and 1979–1991, while no evidence for convergence was found for the post-1991, pre-Kyoto period. We could not find support for coal substituting technologies.

  • 228. Acar, Sevil
    et al.
    Söderholm, Patrik
    Brännlund, Runar
    Umeå University, Faculty of Social Sciences, Center for Environmental and Resource Economics (CERE). Umeå University, Faculty of Social Sciences, Umeå School of Business and Economics (USBE).
    Convergence of per capita carbon dioxide emissions: implications and meta-analysis2018In: Climate Policy, ISSN 1469-3062, E-ISSN 1752-7457, Vol. 18, no 4, p. 512-525Article in journal (Refereed)
    Abstract [en]

    There is a rich empirical literature testing whether per capita carbon dioxide emissions tend to converge over time and across countries. This article provides a meta-analysis of the results from this research, and discusses how carbon emissions convergence may be understood in, for instance, the presence of international knowledge spillovers and policy convergence. The results display evidence of either divergence or persistent gaps at the global level, but convergence of per capita carbon dioxide emissions between richer industrialized countries. However, the results appear sensitive to the choice of data sample and choice of convergence concept, e.g. stochastic convergence versus β-convergence. Moreover, peer-reviewed studies have a higher likelihood of reporting convergence in carbon dioxide emissions compared to non-refereed work.

  • 229. Accinelli, Cesare
    et al.
    Saccà, Maria Ludovica
    Batisson, Isabelle
    Fick, Jerker
    Umeå University, Faculty of Science and Technology, Department of Chemistry.
    Mencarelli, Mariangela
    Grabic, Roman
    Removal of oseltamivir (Tamiflu) and other selected pharmaceuticals from wastewater using a granular bioplastic formulation entrapping propagules of Phanerochaete chrysosporium2010In: Chemosphere, ISSN 0045-6535, E-ISSN 1879-1298, Vol. 81, no 3, p. 436-43Article in journal (Refereed)
    Abstract [en]

    The capacity of the ligninolytic fungus Phanerochaete chrysosporium to degrade a wide variety of environmentally persistent xenobiotics has been largely reported in the literature. Beside other factors, one barrier to a wider use of this bioremediation fungus is the availability of effective formulations that ensure easy preparation, handling and application. In this series of laboratory experiments, we evaluated the efficiency of a granular bioplastic formulation entrapping propagules of P. chrysosporium for removal of four selected pharmaceuticals from wastewater samples. Addition of inoculated granules to samples of the wastewater treatment plant of Bologna significantly increased the removal of the antiviral drug oseltamivir (Tamiflu), and the antibiotics, erythromycin, sulfamethoxazol, and ciprofloxacin. Similar effects were also observed in effluent water. Oseltamivir was the most persistent of the four active substances. After 30d of incubation, approximately two times more oseltamivir was removed in bioremediated wastewater than controls. The highest removal efficiency of the bioplastic formulation was observed with the antibiotic ciprofloxacin. Microbiological DNA-based analysis showed that the bioplastic matrix supported the growth of P. chrysosporium, thus facilitating its adaptation to unusual environment such as wastewater.

  • 230. Accinelli, Cesare
    et al.
    Saccà, Maria Ludovica
    Fick, Jerker
    Umeå University, Faculty of Science and Technology, Department of Chemistry.
    Mencarelli, Mariangela
    Lindberg, Richard
    Umeå University, Faculty of Science and Technology, Department of Chemistry.
    Olsen, Björn
    Dissipation and removal of oseltamivir (Tamiflu) in different aquatic environments2010In: Chemosphere, ISSN 0045-6535, E-ISSN 1879-1298, Vol. 79, no 8, p. 891-897Article in journal (Refereed)
    Abstract [en]

    The antiviral drug oseltamivir (Tamiflu) has received recent attention due to the potential use as a first-line defense against H5N1 and H1N1 influenza viruses. Research has shown that oseltamivir is not removed during conventional wastewater treatments, thus having the potential to enter surface water bodies. A series of laboratory experiments investigated the fate and the removal of oseltamivir in two surface water ecosystems of Japan and in a municipal wastewater treatment plant located in Northern Italy. Persistence of oseltamivir in surface water ranged from non-detectable degradation to a half-life of 53d. After 40d, <3% of radiolabeled oseltamivir evolved as (14)CO(2). The presence of sediments (5%) led to a significant increase of oseltamivir degradation and mineralization rates. A more intense mineralization was observed in samples of the wastewater treatment plant when applying a long incubation period (40d). More precisely, 76% and 37% of the initial radioactivity applied as (14)C-oseltamivir was recovered as (14)CO(2) from samples of the biological tank and effluent water, respectively. Two bacterial strains growing on oseltamivir as sole carbon source were isolated and used for its removal from synthetic medium and environmental samples, including surface water and wastewater. Inoculation of water and wastewater samples with the two oseltamivir-degrading strains showed that mineralization of oseltamivir was significantly higher in both inoculated water and wastewater, than in uninoculated controls. Denaturing gradient gel electrophoresis and quantitative PCR analysis showed that Tamiflu would not affect the microbial population of surface water and wastewater.

  • 231. Accordini, Simone
    et al.
    Calciano, Lucia
    Johannessen, Ane
    Portas, Laura
    Benediktsdóttir, Bryndis
    Bertelsen, Randi Jacobsen
    Bråbäck, Lennart
    Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Occupational and Environmental Medicine.
    Carsin, Anne-Elie
    Dharmage, Shyamali C.
    Dratva, Julia
    Forsberg, Bertil
    Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Occupational and Environmental Medicine.
    Gomez Real, Francisco
    Heinrich, Joachim
    Holloway, John W.
    Holm, Mathias
    Janson, Christer
    Jögi, Rain
    Leynaert, Bénédicte
    Malinovschi, Andrei
    Marcon, Alessandro
    Martínez-Moratalla Rovira, Jesús
    Raherison, Chantal
    Sánchez-Ramos, José Luis
    Schlünssen, Vivi
    Bono, Roberto
    Corsico, Angelo G.
    Demoly, Pascal
    Dorado Arenas, Sandra
    Nowak, Dennis
    Pin, Isabelle
    Weyler, Joost
    Jarvis, Deborah
    Svanes, Cecilie
    A three-generation study on the association of tobacco smoking with asthma2018In: International Journal of Epidemiology, ISSN 0300-5771, E-ISSN 1464-3685, Vol. 47, no 4, p. 1106-1117Article in journal (Refereed)
    Abstract [en]

    Background: Mothers' smoking during pregnancy increases asthma risk in their offspring. There is some evidence that grandmothers' smoking may have a similar effect, and biological plausibility that fathers' smoking during adolescence may influence offspring's health through transmittable epigenetic changes in sperm precursor cells. We evaluated the three-generation associations of tobacco smoking with asthma.

    Methods: Between 2010 and 2013, at the European Community Respiratory Health Survey III clinical interview, 2233 mothers and 1964 fathers from 26 centres reported whether their offspring (aged ≤51 years) had ever had asthma and whether it had coexisted with nasal allergies or not. Mothers and fathers also provided information on their parents' (grandparents) and their own asthma, education and smoking history. Multilevel mediation models within a multicentre three-generation framework were fitted separately within the maternal (4666 offspring) and paternal (4192 offspring) lines.

    Results: Fathers' smoking before they were 15 [relative risk ratio (RRR) = 1.43, 95% confidence interval (CI): 1.01-2.01] and mothers' smoking during pregnancy (RRR = 1.27, 95% CI: 1.01-1.59) were associated with asthma without nasal allergies in their offspring. Grandmothers' smoking during pregnancy was associated with asthma in their daughters [odds ratio (OR) = 1.55, 95% CI: 1.17-2.06] and with asthma with nasal allergies in their grandchildren within the maternal line (RRR = 1.25, 95% CI: 1.02-1.55).

    Conclusions: Fathers' smoking during early adolescence and grandmothers' and mothers' smoking during pregnancy may independently increase asthma risk in offspring. Thus, risk factors for asthma should be sought in both parents and before conception.

  • 232. Acharya, Shravan S.
    et al.
    Easton, Christopher D.
    McCoy, Thomas M.
    Spiccia, Leone
    Ohlin, C. André
    Umeå University, Faculty of Science and Technology, Department of Chemistry. School of Chemistry, Monash University, Clayton, Australia.
    Winther-Jensen, Bjorn
    Diverse composites of metal-complexes and PEDOT facilitated by metal-free vapour phase polymerization2017In: Reactive & functional polymers, ISSN 1381-5148, E-ISSN 1873-166X, Vol. 116, p. 101-106Article in journal (Refereed)
    Abstract [en]

    Abstract Oxidative polymerization for the manufacture of conducting polymers such as poly(3,4-ethylenedioxy-thiophene) has traditionally employed iron(III) salts. Demonstrated in this study is vapour phase polymerization of 3,4-ethylenedio- xythiophene using a metal-free oxidant, ammonium persulfate, leading to films with an estimated conductivity of 75 S/cm. Additionally, a route for embedding active transition metal complexes into these poly(3,4-ethylenedioxythiophene)/-poly(styrene-4-sulfonate) (PEDOT/PSS) films via vapour assisted complexation is outlined. Here, the vapour pressure of solid ligands around their melting temperatures was exploited to ensure complexation to metal ions added into the oxidant mixture prior to polymerization of PEDOT. Four composite systems are discussed, viz. PEDOT/PSS embedded with tris(8-hydroxyquinolinato)cobalt(III), tris(2,2-bipyridine)cobalt(II), tris(1,10- phenanthroline)cobalt(II) and tris(8-hyd-roxyquinolinato)aluminium(III). Using these composites, electrochemical reduction of nitrite to ammonia with a faradaic efficiency of 61% was reported.

  • 233. Acharya, Shravan
    et al.
    Winther-Jensen, Bjorn
    Spiccia, Leone
    Ohlin, André C.
    Umeå University, Faculty of Science and Technology, Department of Chemistry.
    Rates of water exchange in 2,2'-bipyridine and 1,10-phenanthroline complexes of CoII and MnII2017In: Australian journal of chemistry (Print), ISSN 0004-9425, E-ISSN 1445-0038, Vol. 70, no 6, p. 751-754Article in journal (Refereed)
    Abstract [en]

    The rates and activation parameters of water exchange at pH 3.0 have been determined using variable temperature 17O NMR spectroscopy for four CoII complexes and one MnII complex: [Co(bpy)(H2O)4]2+, [Co(bpy)2 (H2O)2]2+, [Co(phen)-(H2O)4]2+, [Co(phen)2 (H2O)2]2+, and [Mn(bpy)(H2O)4]2+ (bpy = 2,2′-bipyridyl and phen = 1,10-phenanthroline). Substitution of aquo ligands with 1,10-phenanthroline or 2,2′-bipyridyl leads to an increase in the rate of exchange in the manganese complexes, from k298 (1.8 ± 0.1) × 107 s-1 for [Mn(H2O)6]2+ to (7.2 ± 0.3) × 107 s-1 for [Mn(phen)2 (H2O)2]2+, whereas the trends are more complex for the cobalt complexes. We have used the new data in conjunction with literature data for similar complexes to analyse the effect of M–OH2 distance and degree of substitution.

  • 234. Achouiti, A.
    et al.
    Vogl, T.
    Urban, Constantin
    Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS).
    Hommes, T. J.
    van Zoelen, M. A.
    Florquin, S.
    Roth, J.
    van 't Veer, C.
    de Vos, A. F.
    van der Poll, T.
    Myeloid related protein (mrp) 8/14 contributes to an antibacterial host response against klebsiella (k.) pneumoniae2012In: Shock, ISSN 1073-2322, E-ISSN 1540-0514, Vol. 37, no S1, p. 56-56Article in journal (Other academic)
  • 235. Achouiti, Ahmed
    et al.
    Vogl, Thomas
    Urban, Constantin F
    Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Clinical Bacteriology. Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS).
    Röhm, Marc
    Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS). Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Clinical Bacteriology.
    Hommes, Tijmen J
    van Zoelen, Marieke AD
    Florquin, Sandrine
    Roth, Johannes
    van't Veer, Cornelis
    de Vos, Alex F
    van der Poll, Tom
    Myeloid-related protein-14 contributes to protective immunity in gram-negative pneumonia derived sepsis2012In: PLoS Pathogens, ISSN 1553-7374, Vol. 8, no 10, p. e1002987-Article in journal (Refereed)
    Abstract [en]

    Klebsiella (K.) pneumoniae is a common cause of pneumonia-derived sepsis. Myeloid related protein 8 (MRP8, S100A8) and MRP14 (S100A9) are the most abundant cytoplasmic proteins in neutrophils. They can form MRP8/14 heterodimers that are released upon cell stress stimuli. MRP8/14 reportedly exerts antimicrobial activity, but in acute fulminant sepsis models MRP8/14 has been found to contribute to organ damage and death. We here determined the role of MRP8/14 in K. pneumoniae sepsis originating from the lungs, using an established model characterized by gradual growth of bacteria with subsequent dissemination. Infection resulted in gradually increasing MRP8/14 levels in lungs and plasma. Mrp14 deficient (mrp14(-/-)) mice, unable to form MRP8/14 heterodimers, showed enhanced bacterial dissemination accompanied by increased organ damage and a reduced survival. Mrp14(-/-) macrophages were reduced in their capacity to phagocytose Klebsiella. In addition, recombinant MRP8/14 heterodimers, but not MRP8 or MRP14 alone, prevented growth of Klebsiella in vitro through chelation of divalent cations. Neutrophil extracellular traps (NETs) prepared from wildtype but not from mrp14(-/-) neutrophils inhibited Klebsiella growth; in accordance, the capacity of human NETs to kill Klebsiella was strongly impaired by an anti-MRP14 antibody or the addition of zinc. These results identify MRP8/14 as key player in protective innate immunity during Klebsiella pneumonia.

  • 236.
    Achour, Cyrinne
    et al.
    Umeå University, Faculty of Medicine, Department of Medical Biosciences. Umeå University, Faculty of Medicine, Wallenberg Centre for Molecular Medicine at Umeå University (WCMM).
    Aguilo, Francesca
    Umeå University, Faculty of Medicine, Department of Medical Biosciences. Umeå University, Faculty of Medicine, Wallenberg Centre for Molecular Medicine at Umeå University (WCMM).
    Long non-coding RNA and Polycomb: an intricate partnership in cancer biology2018In: Frontiers in Bioscience, ISSN 1093-9946, E-ISSN 1093-4715, Vol. 23, p. 2106-2132Article in journal (Refereed)
    Abstract [en]

    High-throughput analyses have revealed that the vast majority of the transcriptome does not code for proteins. These non-translated transcripts, when larger than 200 nucleotides, are termed long non-coding RNAs (lncRNAs), and play fundamental roles in diverse cellular processes. LncRNAs are subject to dynamic chemical modification, adding another layer of complexity to our understanding of the potential roles that lncRNAs play in health and disease. Many lncRNAs regulate transcriptional programs by influencing the epigenetic state through direct interactions with chromatin-modifying proteins. Among these proteins, Polycomb repressive complexes 1 and 2 (PRC1 and PRC2) have been shown to be recruited by lncRNAs to silence target genes. Aberrant expression, deficiency or mutation of both lncRNA and Polycomb have been associated with numerous human diseases, including cancer. In this review, we have highlighted recent findings regarding the concerted mechanism of action of Polycomb group proteins (PcG), acting together with some classically defined lncRNAs including X-inactive specific transcript (XIST), antisense non-coding RNA in the INK4 locus (ANRIL), metastasis associated lung adenocarcinoma transcript 1 (MALAT1), and HOX transcript antisense RNA (HOTAIR).

  • 237.
    Aciz, Michel
    et al.
    Umeå University, Faculty of Medicine, Department of Odontology.
    Akhter, Mahfuz
    Umeå University, Faculty of Medicine, Department of Odontology.
    Anti-Tumor Necrosis Factor-α Treatment in Periodontal Disease – a literature review2016Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    The aim of this literature review was to summarize human studies on the effects of tumor necrosis factor-α (TNF-α) inhibitors on periodontal status. Today TNF-α inhibitors is an established treatment method with positive effects in Rheumatoid arthritis (RA) a disease similar to periodontitis. TNF-α inhibitors have in animal studies demonstrated positive results in experimental periodontitis.

     Literature search was made in PubMed. Included articles were clinical human studies examining the effects on bleeding on probing (BOP), gingival index (GI) or modified gingival index (MGI), clinical attachment level (CAL) and periodontal probing depth (PPD) in patients with autoimmune diseases and treated with TNF-α inhibitors. Animal studies and studies in languages other than English were excluded. The guidelines of The Swedish Council on Technology Assessment in Health Care (SBU) were used to evaluate the level of evidence in the included articles and they were graded according to the GRADE system.

    The search rendered 264 articles of which 17 were selected for abstract reading. Eight of these were read in full-text; one randomized clinical trial (RCT) of moderate quality, two cross-sectional studies and five longitudinal studies of low evidence.  Patients treated with anti-TNF-α compared to the untreated reference groups in the two cross-sectional studies demonstrated lower BOP and GI, while diverging results on attachment loss were shown. The longitudinal studies demonstrated diverging effects of anti-TNF-α treatment on all periodontal parameters. The RCT showed that anti-TNF-α as a complement to conventional periodontal treatment had positive effects on CAL, PPD, BOP and GI while anti-TNF-α treatment alone lacked significant effects on these parameters. There are limited publications within the field and the studies show heterogeneity in terms of study design. Anti-TNF-α treatment showed small significant positive effects on the periodontal parameters in some studies but diverging results were noted. Studies of sufficient scientific quality specifically addressing the effect of anti-TNF-α treatment on periodontitis are needed to elucidate the effects of anti-TNF-α treatment in periodontitis.

  • 238.
    Ackebo, Klas
    Umeå University, Faculty of Social Sciences, Department of Psychology.
    Motivation och engagemang hos innebandytränare: - Ur ett Self-determination Theory-perspektiv2016Independent thesis Advanced level (degree of Master (One Year)), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    According to Self-determination Theory, people perform better when they experience inner motivation, rather than motivation sprung from external factors. This study aims to examine motivation in floorball coaches. Especially, reasons for starting to work as a coach are examined, as well as relationships between those reasons and the coaches experienced type of motivation. A web-survey was sent to 1059 floorball coaches in Sweden, wich were answered by 355 coaches. The results show 12 reasons why the coaches chose to commit themselves: Their own children, Sports interest, Contribute, Lack of coaches, Enjoyment, Their own development, A general interest, Leadership interest, Social connections, Asked to, Possessed expertise and A good cause. The results also showed that the coaches experienced inner motivation to the largest extent. Several relationships were shown between eight of the categories and the different types of motivation. Of those, the most distinct was the relationship between Contribute and self-determined motivation. This may be due to that the will to contribute can be related to the psychological need for relatedness. The study contributes to the knowledge of factors that may affect inner motivation in athletic coaches.

  • 239.
    Ackemo, Annelie
    et al.
    Umeå University, Faculty of Social Sciences, Basic training programme for Police Officers.
    Nilsson, Susanne
    Umeå University, Faculty of Social Sciences, Basic training programme for Police Officers.
    Mobbing och kränkande behandling: Hur agerar skolan2003Independent thesis Basic level (professional degree), 5 credits / 7,5 HE creditsStudent thesis
    Abstract [sv]

    Många svenska skolor har problem med våld, mobbing, skadegörelse och stölder. Enligt skolverkets attitydundersökning uppgav fyra procent av eleverna att de kände sig mobbare av andra elever medan sex procent kände sig mobbade av lärare. Mobbningen kan få svåra följder som t.ex. låg självkänsla. Skolans agerande mot mobbning och kränkande behandling regleras i ett antal styrdokument, främst Skollagen och Läroplanen, som anger de mål och riktlinjer skolan ska följa. Skolan har skyldighet att upprätta en handlingsplan som beskriver de åtgärder som skall vidtas för att förebygga och motverka mobbing och annan kränkande behandling. Ett problem som uppmärksammats är att många av skolans handlingsplaner uppvisar brister när det gäller de förebyggande åtgärderna samt uppföljningen av programmen. Alla som arbetar i skolan har skyldighet att aktivt motverka trakasserier och förtryck. Det är viktigt att kartlägga innebörden i begreppen mobbing och kränkande behandling för att veta vart gränsen går och vilka förebyggande åtgärder som är möjliga. I arbetet belyser vi frågeställningar så som; Vad är mobbing och kränkande behandling? Upplevs mobbing som ett problem i skolorna? Förekommer samverkan med andra myndigheter? Idet förebyggande arbetet är samverkan en förutsättning för att på ett effektivt sätt kunna motverka mobbing och kränkande behandling…

  • 240.
    Acosta Bogran, Paola
    et al.
    Umeå University, Faculty of Social Sciences, Umeå School of Business and Economics (USBE), Business Administration.
    Džaja, Daria
    Umeå University, Faculty of Social Sciences, Umeå School of Business and Economics (USBE), Business Administration.
    How do SMEs engage in Green Public Procurement?: An exploratory study of SMEs' barriers and enablers for Green Public Procurement in Scotland.2015Independent thesis Advanced level (degree of Master (One Year)), 10 credits / 15 HE creditsStudent thesis
  • 241.
    Acosta, Naydú
    et al.
    Universidad Industrial de Santander .
    Pollard, Jennifer
    National University of Colombia.
    Mosquera, Paola
    Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Epidemiology and Global Health.
    Reveiz, Ludovic
    National University of Colombia.
    Equidad en el desarrollo de guias de practica clinica: [The concept of equity when developing clinical practice guidelines]2011In: Revista de Salud Pública, ISSN 0124-0064, Vol. 13, no 2, p. 327-38Article in journal (Refereed)
    Abstract [en]

    This systematic literature review sought to identify methodologies and technical strategies emphasising healthcare services and outcomes when incorporating the concept of equity into Clinical Practice Guidelines (CPG). 940 references were identified, of which 20 fulfilling the inclusion criteria were selected. While no reports were found describing or evaluating an explicit methodology for incorporating considerations of equity into CPG, some studies revealed related strategies or processes, summarised as follows: 1. Target population involvement during all phases of designing, implementing and evaluating CPG; 2. "Cultural capacity" seen as being necessary in CPGs' "cultural translation" for interventions to have less disparity regarding their application and results; 3. Considering psycho-social factors which could affect implementing CPG, and; 4. Considering system inequities so that any health intervention would also confront risks and obstacles to health care due to socioeconomic status. It was concluded that CPGs could be a potential route for promoting more equitable healthcare effects by standardising health interventions if, by incorporating some of the processes described above, they actively seek to avoid unjust differences in access to and/or the quality of the interventions that they prescribe.

  • 242.
    Acosta Romero, Carolin
    Umeå University, Faculty of Medicine, Department of Community Medicine and Rehabilitation, Occupational Therapy.
    Arbetsterapeutiska interventioner vid depression – en systematisk litteraturöversikt: Occupational therapy interventions for depression - a systematic literature review2017Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [sv]

    Depression kan drabba personer i alla åldrar och är en av de vanligaste psykiska sjukdomarna i Sverige och i världen. I Sverige drabbas ca 4-10% av befolkningen av depression och symtom kan återkomma flera gånger under en livstid, vilket beskrivs som depressiva episoder. Sjukdomstillståndet kan leda till nedsatt livskvalitet som orsakas av depressiva symtom som nedstämdhet, minskat intresse, aptitförändring, sömnsvårigheter, energilöshet, pessimistiska tankar, koncentrationssvårigheter och självmordstankar. Studiens syfte var att identifiera och beskriva effekten av arbetsterapeutiska interventioner vid diagnostiserad depression samt att belysa upplevelsen av dessa interventioner.  Nio vetenskapliga artiklar granskades och analyserades i en litteraturöversikt. Endast vuxna deltagare med egentlig depression som primär diagnos inkluderades i sökning i databaserna PsycINFO, Academic Search Elite, PubMed, - OT-Seeker och CINAHL. Resultatet presenterar aktivitetsbaserade interventioner för att hantera vardagen så som arbetsinriktade interventioner, skapande aktiviteter och programbaserade interventioner. Effekter av dessa arbetsterapeutiska interventioner beskrivs förbättra klientens livskvalitet, minska depressions symtom, öka känsla av delaktighet och tillfredställelse i vardagliga aktiviteter, samt främja klientens återgång till arbete utan att uppleva ökad stress. Författaren i den här studien anser att det kan vara av stor betydelse för yrkesverksamma arbetsterapeuter att använda sig av aktivitetsbaserade interventioner som känns meningsfulla och tillfredställande för klienten. Ytterligare forskning behövs dock, då det finns för få studier som undersöker effekten av arbetsterapeutiska interventioner för personer med depression och upplevelse av att leva med depression. 

  • 243. Acosta, Stefan
    et al.
    Block, Tomas
    Björnsson, Steinarr
    Resch, Timothy
    Björck, Martin
    Nilsson, Torbjörn K
    Department of Clinical Chemistry, Örebro University Hospital.
    Diagnostic pitfalls at admission in patients with acute superior mesenteric artery occlusion2012In: Journal of Emergency Medicine, ISSN 0736-4679, E-ISSN 1090-1280, Vol. 42, no 6, p. 635-641Article in journal (Refereed)
    Abstract [en]

    BACKGROUND: Acute superior mesenteric artery (SMA) occlusion leads to acute intestinal ischemia and is associated with high mortality. Early diagnosis is often missed, and confounding factors leading to diagnostic delays need to be highlighted. OBJECTIVES: To identify potential diagnostic laboratory pitfalls at admission in patients with acute SMA occlusion. METHODS: Fifty-five patients with acute SMA occlusion were identified from the in-hospital register during a 4-year period, 2005-2009. RESULTS: The median age was 76 years; 78% were women. The occlusion was embolic in 53% and thrombotic in 47% of patients. At admission, troponin I was above the clinical decision level (> 0.06 μg/L) for acute ischemic myocardial injury in 9/19 (47%) patients with embolic occlusion. Elevated pancreas amylase and normal plasma lactate were found in 12/45 and 13/27, respectively. A troponin I (TnI) above the clinical decision level was associated with a high frequency of referrals from the general surgeon to a specialist in internal medicine (p = 0.011) or a cardiologist (p = 0.024). The diagnosis was established after computed tomography angiography in 98% of the patients. The overall in-hospital mortality rate was 33%. Attempting intestinal revascularization (n = 43; p < 0.001), with a 95% frequency rate of completion control of the vascular procedure, was associated with a higher survival rate, whereas referral to the cardiologist was associated with a higher mortality rate (p = 0.018). CONCLUSION: Elevated TnI was common in acute SMA occlusion, and referral to the cardiologist was found to be associated with adverse outcome. Elevated pancreas amylase and normal plasma lactate values are also potential pitfalls at admission in patients with acute SMA occlusion.

  • 244. Acosta, Stefan
    et al.
    Nilsson, Torbjörn K
    Department of Clinical Chemistry, Örebro University Hospital.
    Current status on plasma biomarkers for acute mesenteric ischemia2012In: Journal of Thrombosis and Thrombolysis, ISSN 0929-5305, E-ISSN 1573-742X, Vol. 33, no 4, p. 355-361Article in journal (Refereed)
    Abstract [en]

    Clinical diagnosis of acute mesenteric ischemia is difficult. The aim of this review is to provide current status on the search for an accurate plasma biomarker for acute mesenteric ischemia. A search using the medical subject heading terms marker and mesenteric ischemia or intestinal ischemia or superior mesenteric artery occlusion or mesenteric venous thrombosis in the Medline and Embase databases from 1980 to 2011. Studies without a control group or a control group consisted of healthy individuals (human studies), or studies on intestinal reperfusion were excluded. Twenty animal and twelve human studies were identified. In human studies, the studied series of patients had a control group that had a need of laparotomy (n = 2), suspected acute mesenteric ischemia (n = 7), acute abdomen (n = 2) or systemic inflammatory response syndrome (n = 1). D: -dimer has been found to be the most consistent highly sensitive early marker, but specificity was low. The follow-up study on α-glutathione S-transferase yielded inferior sensitivity and accuracy than the preliminary study, clearly questioning the value of this marker. Intestinal fatty acid binding globulin (I-FABP) and D: -lactate are both interesting markers, but the results were conflicting. Different cut-off levels have been used in the studies on I-FABP. The encouraging preliminary result of cobalt-albumin and urinary FABP as an accurate marker needs to be addressed in other study populations. The early clinical and laboratory diagnosis of intestinal ischemia remains a challenge. None of the proposed plasma-derived tests for acute mesenteric ischemia has as yet entered routine clinical practice. The proposed biomarkers need to be evaluated in a prospective clinical research project in patients with acute abdomen.

  • 245. Adam, Martin
    et al.
    Schikowski, Tamara
    Carsin, Anne Elie
    Cai, Yutong
    Jacquemin, Benedicte
    Sanchez, Margaux
    Vierkötter, Andrea
    Marcon, Alessandro
    Keidel, Dirk
    Sugiri, Dorothee
    Al Kanani, Zaina
    Nadif, Rachel
    Siroux, Valérie
    Hardy, Rebecca
    Kuh, Diana
    Rochat, Thierry
    Bridevaux, Pierre-Olivier
    Eeftens, Marloes
    Tsai, Ming-Yi
    Villani, Simona
    Phuleria, Harish Chandra
    Birk, Matthias
    Cyrys, Josef
    Cirach, Marta
    de Nazelle, Audrey
    Nieuwenhuijsen, Mark J
    Forsberg, Bertil
    Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Occupational and Environmental Medicine.
    de Hoogh, Kees
    Declerq, Christophe
    Bono, Roberto
    Piccioni, Pavilio
    Quass, Ulrich
    Heinrich, Joachim
    Jarvis, Deborah
    Pin, Isabelle
    Beelen, Rob
    Hoek, Gerard
    Brunekreef, Bert
    Schindler, Christian
    Sunyer, Jordi
    Krämer, Ursula
    Kauffmann, Francine
    Hansell, Anna L
    Künzli, Nino
    Probst-Hensch, Nicole
    Adult lung function and long-term air pollution exposure. ESCAPE: a multicentre cohort study and meta-analysis2015In: European Respiratory Journal, ISSN 0903-1936, E-ISSN 1399-3003, Vol. 41, no 5, p. 38-50Article in journal (Refereed)
    Abstract [en]

    The chronic impact of ambient air pollutants on lung function in adults is not fully understood. The objective of this study was to investigate the association of long-term exposure to ambient air pollution with lung function in adult participants from five cohorts in the European Study of Cohorts for Air Pollution Effects (ESCAPE). Residential exposure to nitrogen oxides (NO2, NOx) and particulate matter (PM) was modelled and traffic indicators were assessed in a standardised manner. The spirometric parameters forced expiratory volume in 1 s (FEV1) and forced vital capacity (FVC) from 7613 subjects were considered as outcomes. Cohort-specific results were combined using meta-analysis. We did not observe an association of air pollution with longitudinal change in lung function, but we observed that a 10 μg·m(-3) increase in NO2 exposure was associated with lower levels of FEV1 (-14.0 mL, 95%CI -25.8- -2.1) and FVC (-14.9 mL, 95% CI -28.7- -1.1). An increase of 10 μg·m(-3) in PM10, but not other PM metrics (PM2.5, coarse fraction of PM, PM absorbance), was associated with a lower level of FEV1 (-44.6 mL, 95% CI -85.4- -3.8) and FVC (-59.0 mL, 95% CI -112.3- -5.6). The associations were particularly strong in obese persons. This study adds to the evidence for an adverse association of ambient air pollution with lung function in adults at very low levels in Europe.

  • 246. Adam, Zach
    et al.
    Adamska, Iwona
    Nakabayashi, Kazumi
    Ostersetzer, Oren
    Haussuhl, Kirsten
    Manuell, Andrea
    Zheng, Bo
    Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
    Vallon, Olivier
    Rodermel, Steven
    Shinozaki, Kazuo
    Chloroplast and mitochondrial proteases in Arabidopsis: a proposed nomenclature2001In: Plant Physiology, ISSN 0032-0889, E-ISSN 1532-2548, Vol. 125, no 4, p. 1912-1918Article in journal (Refereed)
    Abstract [en]

    The identity and scope of chloroplast and mitochondrial proteases in higher plants has only started to become apparent in recent years. Biochemical and molecular studies suggested the existence of Clp, FtsH, and DegP proteases in chloroplasts, and a Lon protease in mitochondria, although currently the full extent of their role in organellar biogenesis and function remains poorly understood. Rapidly accumulating DNA sequence data, especially from Arabidopsis, has revealed that these proteolytic enzymes are found in plant cells in multiple isomeric forms. As a consequence, a systematic approach was taken to catalog all these isomers, to predict their intracellular location and putative processing sites, and to propose a standard nomenclature to avoid confusion and facilitate scientific communication. For the Clp protease most of the ClpP isomers are found in chloroplasts, whereas one is mitochondrial. Of the ATPase subunits, the one ClpD and two ClpC isomers are located in chloroplasts, whereas both ClpX isomers are present in mitochondria. Isomers of the Lon protease are predicted in both compartments, as are the different forms of FtsH protease. DegP, the least characterized protease in plant cells, has the most number of isomers and they are predicted to localize in several cell compartments. These predictions, along with the proposed nomenclature, will serve as a framework for future studies of all four families of proteases and their individual isomers.

  • 247.
    Adama, Blekou
    Umeå University, Faculty of Social Sciences, Department of Geography and Economic History, Economic and social geography.
    Assessing the walking access to bus stops in Umeå urban area and the relationship with the socio-economic characteristics2018Independent thesis Advanced level (degree of Master (One Year)), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    To reduce greenhouse gas emissions in the atmosphere and protect the environment, Swedish cities are heavily invested in sustainable development by developing pedestrian roads and intensifying public transport. Their goal is to make the population less dependent on cars by facilitating accessibility to transit transport. The urban area of ​​Umeå (Sweden), which is experiencing an annual increasing of it population due to urban development, includes 244 km of pedestrian or bike routes and an intensified bus network. The purpose of this paper is to evaluate the walking accessibility of the population at bus stops and to analyze the socio-economic relationship. The use of GIS tools allowed to calculate the proportion of the population and the houses around the bus stop, the average distance accessibility. The use of 3D allowed the observation of access constraints related to the topography and to calculate the slope. The use of the multiple regression model has analyzed the relationship between the shortest accessibility distance and the socio-economic factors that are the income, gender and age. The results show that the average distance of access to the bus stop is 186m. 99.2% of the population lives within 800m from the bus stops. The results of the regression showed that income is the main factor that pushes people to take the bus and live in certain types of housing. The observation of the 3d map and the calculation of the slope made it possible to know the neighborhoods established in the hill and whose residents are susceptible to spend more energy than the other inhabitant living on flat ground. The results mean that most people living in the urban area of ​​Umeå have good access to the bus stop whatever the social group, the level of income and the type of housing.

  • 248.
    Adamo, Hanibal
    et al.
    Umeå University, Faculty of Medicine, Department of Medical Biosciences, Pathology.
    Hammarsten, Peter
    Umeå University, Faculty of Medicine, Department of Medical Biosciences, Pathology.
    Hägglöf, Christina
    Umeå University, Faculty of Medicine, Department of Medical Biosciences, Pathology.
    Dahl Scherdin, Tove
    Egevad, Lars
    Granfors, Torvald
    Stattin, Pär
    Umeå University, Faculty of Medicine, Department of Surgical and Perioperative Sciences, Urology and Andrology.
    Halin Bergström, Sofia
    Umeå University, Faculty of Medicine, Department of Medical Biosciences, Pathology.
    Bergh, Anders
    Umeå University, Faculty of Medicine, Department of Medical Biosciences, Pathology.
    Prostate cancer induces C/EBPβ expression in surrounding epithelial cells which relates to tumor aggressiveness and patient outcomeManuscript (preprint) (Other academic)
    Abstract [en]

    Implantation of rat prostate cancer cells into the normal rat prostate results in tumor-stimulating adaptations in the tumor-bearing organ. Similar changes are seen in prostate cancer patients and they are related to outcome. One gene previously found to be upregulated in the non-malignant part of a tumor-bearing prostate lobe in rats was the transcription factor CCAAT/enhancer-binding protein-β (C/EBPβ). To explore this further, we examined C/EBPβ expression by quantitative RT-PCR, immunohistochemistry, and western blot in normal rat prostate tissue surrounding slow-growing non-metastatic Dunning G, rapidly growing poorly metastatic (AT-1), and rapidly growing highly metastatic (MatLyLu) rat prostate tumors―and also by immunohistochemistry in a tissue microarray (TMA) from prostate cancer patients managed by watchful waiting.

    In rats, C/EBPβ mRNA expression was upregulated in the surrounding tumor-bearing prostate lobe. In tumors and in the surrounding non-malignant prostate tissue, C/EBPβ was detected by immunohistochemistry in some epithelial cells and in infiltrating macrophages. The magnitude of glandular epithelial C/EBPβ expression in the tumor-bearing prostates was associated with tumor size, with distance to the tumor, and with tumor cell metastatic capacity.

    In prostate cancer patients, high expression of C/EBPβ in glandular epithelial cells in the surrounding tumor-bearing tissue was associated with accumulation of M1 macrophages (iNOS+) and a favorable outcome. High expression of C/EBPβ in tumor epithelial cells was associated with high Gleason score, high tumor cell proliferation, the presence of metastases at diagnosis, and poor outcome. 

  • 249.
    Adamo, Hanibal Hani
    Umeå University, Faculty of Medicine, Department of Medical Biosciences.
    TINT Tumor Indicating Normal Tissue: new field of diagnostic biomarkers for prostate cancer2016Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    Background: Prostate cancer is the most common cancer in Sweden. Due its highly variable behavior, multifocal nature, and insufficient diagnostic methods, prostate cancer is difficult to diagnose and prognosticate. Some patients have an aggressive lethal disease, but the majority of prostate cancer patients have slow-growing, non-lethal disease with long expected survival without treatment. Current diagnostic methods―serum levels of prostate-specific antigen (PSA) and histological grading of biopsied prostate tissue―often do not give the information required to be able to safely differentiate indolent tumors from potentially lethal ones. Many prostate cancers are difficult to detect by imaging, so tissue biopsy cannot be safely guided towards the tumor, and particularly not towards the most aggressive forms. To overcome this problem, multiple needle biopsies are taken from the organ, but biopsies are small and they sample less than 1% of the whole prostate. In this thesis, we explore the non-malignant prostate tissue adjacent to tumors, which is always sampled in biopsies, and we study adaptive changes in this tissue, which may provide new diagnostic and prognostic markers for prostate cancer. We have therefore proposed that this type of tissue should be termed TINT (Tumor Instructed/indicating Normal Tissue).

     Methods: In our studies, we used orthotopic rat prostate cancer models with tumors of different aggressiveness. We also used clinical materials from patients diagnosed with prostate cancer at transurethral resection (1975‒1990); the majority of these men were followed with watchful waiting. Analyses were performed with whole-genome expression array, quantitative real-time PCR, immunohistochemistry, and western blotting.

     Results: Using the animal model, we found that the presence of a tumor induces changes in gene expression in the surrounding tumor-bearing organ (TINT). The gene signature of TINT was linked to processes such as extracellular matrix organization, immune responses, and inflammation. We also showed that some of these adaptive TINT changes appear to be related to the aggressiveness and metastatic potential of the growing tumor, such as increases in macrophages, in mast cells, in vascular densities, and in vascular cell-proliferation. Some of these findings were confirmed by our observations in patient samples. We found that high staining of the extracellular matrix component hyaluronan in the stroma of the non-malignant prostate tissue was prognostic for short cancer-specific survival. We also found that an elevated proportion of C/EBP-beta positive epithelial cells in non-malignant (TINT) prostate tissue was associated with a good prognosis.

     Conclusions: Using animal experiments and patient samples, we showed that the presence of prostate cancer induces changes in the tumor-bearing organ, alterations associated with tumor aggressiveness, and that grading of these changes in TINT can be used to predict outcome in prostate cancer patients. 

  • 250.
    Adamo, Hanibal Hani
    et al.
    Umeå University, Faculty of Medicine, Department of Medical Biosciences, Pathology.
    Bergström, Sofia Halin
    Umeå University, Faculty of Medicine, Department of Medical Biosciences, Pathology.
    Bergh, Anders
    Umeå University, Faculty of Medicine, Department of Medical Biosciences, Pathology.
    Characterization of a Gene Expression Signature in Normal Rat Prostate Tissue Induced by the Presence of a Tumor Elsewhere in the Organ2015In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 10, no 6, article id e0130076Article in journal (Refereed)
    Abstract [en]

    Implantation of rat prostate cancer cells into the normal rat prostate results in tumor-stimulating changes in the tumor-bearing organ, for example growth of the vasculature, an altered extracellular matrix, and influx of inflammatory cells. To investigate this response further, we compared prostate morphology and the gene expression profile of tumor-bearing normal rat prostate tissue (termed tumor-instructed/indicating normal tissue (TINT)) with that of prostate tissue from controls. Dunning rat AT-1 prostate cancer cells were injected into rat prostate and tumors were established after 10 days. As controls we used intact animals, animals injected with heat-killed AT-1 cells or cell culture medium. None of the controls showed morphological TINT-changes. A rat Illumina whole-genome expression array was used to analyze gene expression in AT-1 tumors, TINT, and in medium injected prostate tissue. We identified 423 upregulated genes and 38 downregulated genes (p<0.05, >= 2-fold change) in TINT relative to controls. Quantitative RT-PCR analysis verified key TINT-changes, and they were not detected in controls. Expression of some genes was changed in a manner similar to that in the tumor, whereas other changes were exclusive to TINT. Ontological analysis using GeneGo software showed that the TINT gene expression profile was coupled to processes such as inflammation, immune response, and wounding. Many of the genes whose expression is altered in TINT have well-established roles in tumor biology, and the present findings indicate that they may also function by adapting the surrounding tumor-bearing organ to the needs of the tumor. Even though a minor tumor cell contamination in TINT samples cannot be ruled out, our data suggest that there are tumor-induced changes in gene expression in the normal tumor-bearing organ which can probably not be explained by tumor cell contamination. It is important to validate these changes further, as they could hypothetically serve as novel diagnostic and prognostic markers of prostate cancer.

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