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Sengupta, P., Jamroskovic, J. & Sabouri, N. (2023). A beginner's handbook to identify and characterize i-motif DNA. In: Hans Renata (Ed.), Methods in enzymology: . Elsevier
Åpne denne publikasjonen i ny fane eller vindu >>A beginner's handbook to identify and characterize i-motif DNA
2023 (engelsk)Inngår i: Methods in enzymology / [ed] Hans Renata, Elsevier, 2023Kapittel i bok, del av antologi (Fagfellevurdert)
Abstract [en]

Genomic DNA exhibits an innate ability to manifest diverse sequence-dependent secondary structures, serving crucial functions in gene regulation and cellular equilibrium. While extensive research has confirmed the formation of G-quadruplex structures by guanine-rich sequences in vitro and in cells, recent investigations have turned the quadruplex community's attention to the cytosine (C)-rich complementary strands that can adopt unique tetra-stranded conformation, termed as intercalated motif or i-motif. I-motifs are stabilized by hemi-protonated C:CH+ base pairs under acidic conditions. Initially, the in vivo occurrence of i-motifs was underestimated because their formation is favored at non-physiological pH. However, groundbreaking research utilizing the structure-specific iMab antibody and high-throughput sequencing have recently detected their conserved dispersion throughout the genome, challenging previous assumptions. Given the evolving nature of this research field, it becomes imperative to conduct independent in vitro experiments aimed at identifying potential i-motif formation in C-rich sequences and consolidating the findings to address the properties of i-motifs. This chapter serves as an introductory guide for the swift identification of novel i-motifs, where we present an experimental framework for investigating and characterizing i-motif sequences in vitro. In this chapter, we selected a synthetic oligonucleotide (C7T3) sequence and outlined appropriate methodologies for annealing the i-motif structure into suitable buffers. Then, we validated its formation by CD (Circular Dichroism) and NMR (Nuclear Magnetic Resonance) spectroscopy. Finally, we provided a thorough account of the step-by-step procedures to investigate the effect of i-motif formation on the stalling or retardation of DNA replication using high resolution primer extension assays.

sted, utgiver, år, opplag, sider
Elsevier, 2023
Serie
Methods in enzymology, ISSN 0076-6879 ; 693
Emneord
Circular dichroism, I-motifs, Nuclear magnetic resonance, Primer extension assay, Quadruplex DNA
HSV kategori
Identifikatorer
urn:nbn:se:umu:diva-218112 (URN)10.1016/bs.mie.2023.11.001 (DOI)2-s2.0-85178602961 (Scopus ID)
Forskningsfinansiär
Swedish Cancer Society, 22 2380 Pj 01HSwedish Research Council, VR-MH 2021-02468Knut and Alice Wallenberg Foundation, KAW 2021.0173The Kempe Foundations, SMK2058Wenner-Gren Foundations, o.UPD2020-0097Åke Wiberg Foundation, M20-0125
Tilgjengelig fra: 2023-12-19 Laget: 2023-12-19 Sist oppdatert: 2023-12-19
Deiana, M., Andrés Castán, J. M., Josse, P., Kahsay, A., Sánchez, D. P., Morice, K., . . . Sabouri, N. (2023). A new G-quadruplex-specific photosensitizer inducing genome instability in cancer cells by triggering oxidative DNA damage and impeding replication fork progression. Nucleic Acids Research, 51(12), 6264-6285
Åpne denne publikasjonen i ny fane eller vindu >>A new G-quadruplex-specific photosensitizer inducing genome instability in cancer cells by triggering oxidative DNA damage and impeding replication fork progression
Vise andre…
2023 (engelsk)Inngår i: Nucleic Acids Research, ISSN 0305-1048, E-ISSN 1362-4962, Vol. 51, nr 12, s. 6264-6285Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Photodynamic therapy (PDT) ideally relies on the administration, selective accumulation and photoactivation of a photosensitizer (PS) into diseased tissues. In this context, we report a new heavy-atom-free fluorescent G-quadruplex (G4) DNA-binding PS, named DBI. We reveal by fluorescence microscopy that DBI preferentially localizes in intraluminal vesicles (ILVs), precursors of exosomes, which are key components of cancer cell proliferation. Moreover, purified exosomal DNA was recognized by a G4-specific antibody, thus highlighting the presence of such G4-forming sequences in the vesicles. Despite the absence of fluorescence signal from DBI in nuclei, light-irradiated DBI-treated cells generated reactive oxygen species (ROS), triggering a 3-fold increase of nuclear G4 foci, slowing fork progression and elevated levels of both DNA base damage, 8-oxoguanine, and double-stranded DNA breaks. Consequently, DBI was found to exert significant phototoxic effects (at nanomolar scale) toward cancer cell lines and tumor organoids. Furthermore, in vivo testing reveals that photoactivation of DBI induces not only G4 formation and DNA damage but also apoptosis in zebrafish, specifically in the area where DBI had accumulated. Collectively, this approach shows significant promise for image-guided PDT.

sted, utgiver, år, opplag, sider
Oxford University Press, 2023
HSV kategori
Identifikatorer
urn:nbn:se:umu:diva-212227 (URN)10.1093/nar/gkad365 (DOI)000988008500001 ()37191066 (PubMedID)2-s2.0-85164253573 (Scopus ID)
Forskningsfinansiär
Swedish Cancer Society, 22 2380 PjSwedish Research Council, VR-MH 2021–02468Knut and Alice Wallenberg Foundation, KAW 2021-0173Swedish Cancer Society, 21 0302 PT 01 HWenner-Gren Foundations, UPD2020-0097Swedish Cancer Society, 20 0827 PjFCancerforskningsfonden i Norrland, LP 22-2312Cancerforskningsfonden i Norrland, LP20 1024 2257Cancerforskningsfonden i Norrland, LP 21–2298Swedish Research Council, 2017-01531Swedish Society of Medicine, SLS-890521Region Västerbotten, RV-930167Sjöberg FoundationKnut and Alice Wallenberg Foundation, KAW 2015.0114Marianne and Marcus Wallenberg Foundation, MMW 2020.0189Swedish Cancer Society, 20 1339 PjF
Tilgjengelig fra: 2023-07-21 Laget: 2023-07-21 Sist oppdatert: 2023-07-21bibliografisk kontrollert
Deiana, M., Chand, K., Chorell, E. & Sabouri, N. (2023). Parallel G-quadruplex DNA structures from nuclear and mitochondrial genomes trigger emission enhancement in a nonfluorescent nano-aggregated fluorine-boron-based dye. Journal of Physical Chemistry Letters, 14(7), 1862-1869
Åpne denne publikasjonen i ny fane eller vindu >>Parallel G-quadruplex DNA structures from nuclear and mitochondrial genomes trigger emission enhancement in a nonfluorescent nano-aggregated fluorine-boron-based dye
2023 (engelsk)Inngår i: Journal of Physical Chemistry Letters, ISSN 1948-7185, E-ISSN 1948-7185, Vol. 14, nr 7, s. 1862-1869Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Molecular self-assembly is a powerful tool for the development of functional nanostructures with adaptive optical properties. However, in aqueous solution, the hydrophobic effects in the monomeric units often afford supramolecular architectures with typical side-by-side π-stacking arrangement with compromised emissive properties. Here, we report on the role of parallel DNA guanine quadruplexes (G4s) as supramolecular disaggregating-capture systems capable of coordinating a zwitterionic fluorine-boron-based dye and promoting activation of its fluorescence signal. The dye's high binding affinity for parallel G4s compared to nonparallel topologies leads to a selective disassembly of the dye's supramolecular state upon contact with parallel G4s. This results in a strong and selective disaggregation-induced emission that signals the presence of parallel G4s observable by the naked eye and inside cells. The molecular recognition strategy reported here will be useful for a multitude of affinity-based applications with potential in sensing and imaging systems.

sted, utgiver, år, opplag, sider
American Chemical Society (ACS), 2023
HSV kategori
Identifikatorer
urn:nbn:se:umu:diva-205385 (URN)10.1021/acs.jpclett.2c03301 (DOI)000936590300001 ()36779779 (PubMedID)2-s2.0-85148521026 (Scopus ID)
Tilgjengelig fra: 2023-03-10 Laget: 2023-03-10 Sist oppdatert: 2023-09-05bibliografisk kontrollert
Jamroskovic, J., Deiana, M. & Sabouri, N. (2022). Probing the folding pathways of four-stranded intercalated cytosine-rich motifs at single base-pair resolution. Biochimie, 199, 81-91
Åpne denne publikasjonen i ny fane eller vindu >>Probing the folding pathways of four-stranded intercalated cytosine-rich motifs at single base-pair resolution
2022 (engelsk)Inngår i: Biochimie, ISSN 0300-9084, E-ISSN 1638-6183, Vol. 199, s. 81-91Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Cytosine-rich DNA can fold into four-stranded intercalated structures called i-motifs (iMs) under acidic conditions through the formation of hemi-protonated C:C+ base pairs. However, the folding and stability of iMs rely on many other factors that are not yet fully understood. Here, we combined biochemical and biophysical approaches to determine the factors influencing iM stability under a wide range of experimental conditions. By using high-resolution primer extension assays, circular dichroism, and absorption spectroscopies, we demonstrate that the stabilities of three different biologically relevant iMs are not dependent on molecular crowding agents. Instead, some of the crowding agents affected overall DNA synthesis. We also tested a range of small molecules to determine their effect on iM stabilization at physiological temperature and demonstrated that the G-quadruplex-specific molecule CX-5461 is also a promising candidate for selective iM stabilization. This work provides important insights into the requirements needed for different assays to accurately study iM stabilization, which will serve as important tools for understanding the contribution of iMs in cell regulation and their potential as therapeutic targets.

sted, utgiver, år, opplag, sider
Elsevier, 2022
Emneord
CX-5461, DNA replication, G-quadruplex DNA, High-resolution primer extension assay, I-motif DNA, pH
HSV kategori
Identifikatorer
urn:nbn:se:umu:diva-194635 (URN)10.1016/j.biochi.2022.04.007 (DOI)000800388200003 ()35452743 (PubMedID)2-s2.0-85129038456 (Scopus ID)
Forskningsfinansiär
Swedish Research Council, 2021-02468Swedish Cancer Society, 2019/126Åke Wiberg Foundation, M20-0125Swedish Cancer Society, 21 0302 PT 01 H
Tilgjengelig fra: 2022-05-12 Laget: 2022-05-12 Sist oppdatert: 2023-09-05bibliografisk kontrollert
Deiana, M., Josse, P., Dalinot, C., Osmolovskyi, A., Marqués, P. S., Castán, J. M., . . . Cabanetos, C. (2022). Site-selected thionated benzothioxanthene chromophores as heavy-atom-free small-molecule photosensitizers for photodynamic therapy. Communications Chemistry, 5, Article ID 142.
Åpne denne publikasjonen i ny fane eller vindu >>Site-selected thionated benzothioxanthene chromophores as heavy-atom-free small-molecule photosensitizers for photodynamic therapy
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2022 (engelsk)Inngår i: Communications Chemistry, E-ISSN 2399-3669, Vol. 5, artikkel-id 142Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Photodynamic therapy is a clinically approved anticancer modality that employs a light-activated agent (photosensitizer) to generate cytotoxic reactive oxygen species (ROS). There is therefore a growing interest for developing innovative photosensitizing agents with enhanced phototherapeutic performances. Herein, we report on a rational design synthetic procedure that converts the ultrabright benzothioxanthene imide (BTI) dye into three heavy-atom-free thionated compounds featuring close-to-unit singlet oxygen quantum yields. In contrast to the BTI, these thionated analogs display an almost fully quenched fluorescence emission, in agreement with the formation of highly populated triplet states. Indeed, the sequential thionation on the BTI scaffold induces torsion of its skeleton reducing the singlet-triplet energy gaps and enhancing the spin-orbit coupling. These potential PSs show potent cancer-cell ablation under light irradiation while remaining non-toxic under dark condition owing to a photo-cytotoxic mechanism that we believe simultaneously involves singlet oxygen and superoxide species, which could be both characterized in vitro. Our study demonstrates that this simple site-selected thionated platform is an effective strategy to convert conventional carbonyl-containing fluorophores into phototherapeutic agents for anticancer PDT.

sted, utgiver, år, opplag, sider
Springer Nature, 2022
HSV kategori
Identifikatorer
urn:nbn:se:umu:diva-200995 (URN)10.1038/s42004-022-00752-x (DOI)000876981400002 ()2-s2.0-85140992916 (Scopus ID)
Forskningsfinansiär
Swedish Cancer Society, 21 0302 PT 01 HSwedish Cancer Society, 2019/126Swedish Research Council, 2021-02468EU, Horizon 2020, 722651
Merknad

We acknowledge the Biochemical Imaging Center at Umeå University and the National Microscopy Infrastructure, NMI (VR-RFI 2019-00217) for providing assistance in microscopy.

Tilgjengelig fra: 2022-11-17 Laget: 2022-11-17 Sist oppdatert: 2022-11-17bibliografisk kontrollert
Deiana, M., Obi, I., Andréasson, M., Tamilselvi, S., Chand, K., Chorell, E. & Sabouri, N. (2021). A Minimalistic Coumarin Turn-On Probe for Selective Recognition of Parallel G-Quadruplex DNA Structures. ACS Chemical Biology, 16(8), 1365-1376
Åpne denne publikasjonen i ny fane eller vindu >>A Minimalistic Coumarin Turn-On Probe for Selective Recognition of Parallel G-Quadruplex DNA Structures
Vise andre…
2021 (engelsk)Inngår i: ACS Chemical Biology, ISSN 1554-8929, E-ISSN 1554-8937, Vol. 16, nr 8, s. 1365-1376Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

G-quadruplex (G4) DNA structures are widespread in the human genome and are implicated in biologically important processes such as telomere maintenance, gene regulation, and DNA replication. Guanine-rich sequences with potential to form G4 structures are prevalent in the promoter regions of oncogenes, and G4 sites are now considered as attractive targets for anticancer therapies. However, there are very few reports of small “druglike” optical G4 reporters that are easily accessible through one-step synthesis and that are capable of discriminating between different G4 topologies. Here, we present a small water-soluble light-up fluorescent probe that features a minimalistic amidinocoumarin-based molecular scaffold that selectively targets parallel G4 structures over antiparallel and non-G4 structures. We showed that this biocompatible ligand is able to selectively stabilize the G4 template resulting in slower DNA synthesis. By tracking individual DNA molecules, we demonstrated that the G4-stabilizing ligand perturbs DNA replication in cancer cells, resulting in decreased cell viability. Moreover, the fast-cellular entry of the probe enabled detection of nucleolar G4 structures in living cells. Finally, insights gained from the structure–activity relationships of the probe suggest the basis for the recognition of parallel G4s, opening up new avenues for the design of new biocompatible G4-specific small molecules for G4-driven theranostic applications.

sted, utgiver, år, opplag, sider
American Chemical Society (ACS), 2021
HSV kategori
Identifikatorer
urn:nbn:se:umu:diva-187118 (URN)10.1021/acschembio.1c00134 (DOI)000697396400009 ()34328300 (PubMedID)2-s2.0-85113337330 (Scopus ID)
Forskningsfinansiär
The Kempe Foundations, SMK-1632Knut and Alice Wallenberg Foundation, KAW2015-0189Swedish Cancer Society, CAN 2019/126Swedish Research Council, 2017-05235Swedish Research Council, 2018-02651
Tilgjengelig fra: 2021-09-01 Laget: 2021-09-01 Sist oppdatert: 2023-03-23bibliografisk kontrollert
Deiana, M., Mosser, M., Le Bahers, T., Dumont, E., Dudek, M., Denis-Quanquin, S., . . . Guy, L. (2021). Light-induced in situ chemical activation of a fluorescent probe for monitoring intracellular G-quadruplex structures. Nanoscale, 13(32), 13795-13808
Åpne denne publikasjonen i ny fane eller vindu >>Light-induced in situ chemical activation of a fluorescent probe for monitoring intracellular G-quadruplex structures
Vise andre…
2021 (engelsk)Inngår i: Nanoscale, ISSN 2040-3364, E-ISSN 2040-3372, Vol. 13, nr 32, s. 13795-13808Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Light-activated functional materials capable of remote control over duplex and G-quadruplex (G4) nucleic acids formation at the cellular level are still very rare. Herein, we report on the photoinduced macrocyclisation of a helicenoid quinoline derivative of binaphthol that selectively provides easy access to an unprecedented class of extended heteroaromatic structures with remarkable photophysical and DNA/RNA binding properties. Thus, while the native bisquinoline precursor shows no DNA binding activity, the new in situ photochemically generated probe features high association constants to DNA and RNA G4s. The latter inhibits DNA synthesis by selectively stabilizing G4 structures associated with oncogenic promoters and telomere repeat units. Finally, the light sensitive compound is capable of in cellulo photoconversion, localizes primarily in the G4-rich sites of cancer cells, competes with a well-known G4 binder and shows a clear nuclear co-localization with the quadruplex specific antibody BG4. This work provides a benchmark for the future design and development of a brand-new generation of light-activated target-selective G4-binders.

sted, utgiver, år, opplag, sider
Royal Society of Chemistry, 2021
HSV kategori
Identifikatorer
urn:nbn:se:umu:diva-187087 (URN)10.1039/d1nr02855c (DOI)000680884100001 ()34477654 (PubMedID)2-s2.0-85113310558 (Scopus ID)
Forskningsfinansiär
Swedish Research Council, 2018-02651Knut and Alice Wallenberg Foundation, KAW2015-0189Swedish Cancer Society, 2019/126
Merknad

Correction: Light-induced in situ chemicalactivation of a fluorescent probe for monitoringintracellular G-quadruplex structures. Nanoscale, 2023, 15, 388. DOI: 10.1039/d2nr90225g

Tilgjengelig fra: 2021-09-03 Laget: 2021-09-03 Sist oppdatert: 2022-12-29bibliografisk kontrollert
Yan, K.-P., Obi, I. & Sabouri, N. (2021). The RGG domain in the C-terminus of the DEAD box helicases Dbp2 and Ded1 is necessary for G-quadruplex destabilization. Nucleic Acids Research, 49(14), 8339-8354
Åpne denne publikasjonen i ny fane eller vindu >>The RGG domain in the C-terminus of the DEAD box helicases Dbp2 and Ded1 is necessary for G-quadruplex destabilization
2021 (engelsk)Inngår i: Nucleic Acids Research, ISSN 0305-1048, E-ISSN 1362-4962, Vol. 49, nr 14, s. 8339-8354Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The identification of G-quadruplex (G4) binding proteins and insights into their mechanism of action are important for understanding the regulatory functions of G4 structures. Here, we performed an unbiased affinity-purification assay coupled with mass spectrometry and identified 30 putative G4 binding proteins from the fission yeast Schizosaccharomyces pombe. Gene ontology analysis of the molecular functions enriched in this pull-down assay included mRNA binding, RNA helicase activity, and translation regulator activity. We focused this study on three of the identified proteins that possessed putative arginine-glycine-glycine (RGG) domains, namely the Stm1 homolog Oga1 and the DEAD box RNA helicases Dbp2 and Ded1. We found that Oga1, Dbp2, and Ded1 bound to both DNA and RNA G4s in vitro. Both Dbp2 and Ded1 bound to G4 structures through the RGG domain located in the C-terminal region of the helicases, and point mutations in this domain weakened the G4 binding properties of the helicases. Dbp2 and Ded1 destabilized less thermostable G4 RNA and DNA structures, and this ability was independent of ATP but dependent on the RGG domain. Our study provides the first evidence that the RGG motifs in DEAD box helicases are necessary for both G4 binding and G4 destabilization.

sted, utgiver, år, opplag, sider
Oxford University Press, 2021
HSV kategori
Identifikatorer
urn:nbn:se:umu:diva-187116 (URN)10.1093/nar/gkab620 (DOI)000692599800043 ()34302476 (PubMedID)2-s2.0-85114315622 (Scopus ID)
Forskningsfinansiär
Wenner-Gren FoundationsKnut and Alice Wallenberg Foundation
Tilgjengelig fra: 2021-09-01 Laget: 2021-09-01 Sist oppdatert: 2023-09-05bibliografisk kontrollert
Deiana, M., Chand, K., Jamroskovic, J., Obi, I., Chorell, E. & Sabouri, N. (2020). A Light‐up Logic Platform for Selective Recognition of Parallel G‐Quadruplex Structures via Disaggregation‐Induced Emission. Angewandte Chemie International Edition, 59(2), 896-902
Åpne denne publikasjonen i ny fane eller vindu >>A Light‐up Logic Platform for Selective Recognition of Parallel G‐Quadruplex Structures via Disaggregation‐Induced Emission
Vise andre…
2020 (engelsk)Inngår i: Angewandte Chemie International Edition, ISSN 1433-7851, E-ISSN 1521-3773, Vol. 59, nr 2, s. 896-902Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The design of turn‐on dyes with optical signals sensitive to the formation of supramolecular structures provides fascinating and underexplored opportunities for G‐quadruplex (G4) DNA detection and characterization. Here, we show a new switching mechanism that relies on the recognition‐driven disaggregation (on‐signal) of an ultrabright coumarin‐quinazoline conjugate. The synthesized probe selectively lights‐up parallel G4 DNA structures via the disassembly of its supramolecular state, demonstrating outputs that are easily integrable into a label free molecular logic system. Finally, our molecule preferentially stains the G4‐rich nucleoli of cancer cells.

sted, utgiver, år, opplag, sider
Wiley-VCH Verlagsgesellschaft, 2020
Emneord
aggregation, biosensor, DNA, G-quadruplex, logic gate
HSV kategori
Forskningsprogram
medicinsk biokemi
Identifikatorer
urn:nbn:se:umu:diva-164662 (URN)10.1002/anie.201912027 (DOI)000497789300001 ()31644837 (PubMedID)2-s2.0-85075533982 (Scopus ID)
Forskningsfinansiär
Knut and Alice Wallenberg Foundation, KAW2015-0189Swedish Research Council, VR-NT 2017-05235Swedish Research Council, VR-MH 2018-02651The Kempe Foundations, SMK-1632
Tilgjengelig fra: 2019-10-25 Laget: 2019-10-25 Sist oppdatert: 2023-09-05bibliografisk kontrollert
Deiana, M., Chand, K., Jamroskovic, J., Das, R. N., Obi, I., Chorell, E. & Sabouri, N. (2020). A Site-Specific Self-Assembled Light-up Rotor Probe for Selective Recognition and Stabilization of c-MYC G-Quadruplex DNA. Nanoscale, 12(24), 12950-12957
Åpne denne publikasjonen i ny fane eller vindu >>A Site-Specific Self-Assembled Light-up Rotor Probe for Selective Recognition and Stabilization of c-MYC G-Quadruplex DNA
Vise andre…
2020 (engelsk)Inngår i: Nanoscale, ISSN 2040-3364, E-ISSN 2040-3372, Vol. 12, nr 24, s. 12950-12957Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Direct and unambiguous evidence of the formation of G-quadruplexes (G4s) in human cells have shown their implication in several key biological events and has emphasized their role as important targets for small-molecule cancer therapeutics. Here, we report on the first example of a self-assembled multitasking molecular-rotor G4-binder able to discriminate between an extensive panel of G4 and non-G4 structures and to selectively light-up (up to 105-fold), bind (nanomolar range), and stabilize the c-MYC promoter G4 DNA. In particular, association with the c-MYC G4 triggers the disassembly of its supramolecular state (disaggregation-induced emission, DIE) and induces geometrical restrictions (motion-induced change in emission, MICE) leading to a significant enhancement of its emission yield. Moreover, this optical reporter is able to selectively stabilize the c-MYC G4 and inhibit DNA synthesis. Finally, by using confocal laser-scanning microscopy (CLSM) we show the ability of this compound to localize primarily in the subnuclear G4-rich compartments of cancer cells. This work provides a benchmark for the future design and development of a new generation of smart sequence-selective supramolecular G4-binders that combine outstanding sensing and stability properties, to be utilized in anti-cancer therapy.

sted, utgiver, år, opplag, sider
Royal Society of Chemistry, 2020
HSV kategori
Forskningsprogram
biokemi; organisk kemi; fysikalisk kemi; cellforskning
Identifikatorer
urn:nbn:se:umu:diva-171513 (URN)10.1039/D0NR03404E (DOI)000545599900025 ()2-s2.0-85087110627 (Scopus ID)
Forskningsfinansiär
Knut and Alice Wallenberg Foundation, KAW2015-0189Swedish Research Council, VR-NT 2017-05235Swedish Research Council, VR-MH 2018-02651The Kempe Foundations, SMK-1632
Tilgjengelig fra: 2020-06-03 Laget: 2020-06-03 Sist oppdatert: 2023-03-24bibliografisk kontrollert
Prosjekter
Det essentiella helikaset Pfh1s roll i arvsmassans integritet [2012-03087_VR]; Umeå universitetDen fyrsträngade DNA strukturens roll i hälsa och sjukdom [2018-02651_VR]; Umeå universitet
Organisasjoner
Identifikatorer
ORCID-id: ORCID iD iconorcid.org/0000-0002-4541-7702