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Wuolikainen, Anna
Publikasjoner (10 av 24) Visa alla publikasjoner
Behzadi, A., Pujol-Calderón, F., Tjust, A. E., Wuolikainen, A., Höglund, K., Forsberg, K., . . . Andersen, P. M. (2021). Neurofilaments can differentiate ALS subgroups and ALS from common diagnostic mimics. Scientific Reports, 11(1), Article ID 22128.
Åpne denne publikasjonen i ny fane eller vindu >>Neurofilaments can differentiate ALS subgroups and ALS from common diagnostic mimics
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2021 (engelsk)Inngår i: Scientific Reports, E-ISSN 2045-2322, Vol. 11, nr 1, artikkel-id 22128Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Delayed diagnosis and misdiagnosis are frequent in people with amyotrophic lateral sclerosis (ALS), the most common form of motor neuron disease (MND). Neurofilament light chain (NFL) and phosphorylated neurofilament heavy chain (pNFH) are elevated in ALS patients. We retrospectively quantified cerebrospinal fluid (CSF) NFL, CSF pNFH and plasma NFL in stored samples that were collected at the diagnostic work-up of ALS patients (n = 234), ALS mimics (n = 44) and controls (n = 9). We assessed the diagnostic performance, prognostication value and relationship to the site of onset and genotype. CSF NFL, CSF pNFH and plasma NFL levels were significantly increased in ALS patients compared to patients with neuropathies & myelopathies, patients with myopathies and controls. Furthermore, CSF pNFH and plasma NFL levels were significantly higher in ALS patients than in patients with other MNDs. Bulbar onset ALS patients had significantly higher plasma NFL levels than spinal onset ALS patients. ALS patients with C9orf72HRE mutations had significantly higher plasma NFL levels than patients with SOD1 mutations. Survival was negatively correlated with all three biomarkers. Receiver operating characteristics showed the highest area under the curve for CSF pNFH for differentiating ALS from ALS mimics and for plasma NFL for estimating ALS short and long survival. All three biomarkers have diagnostic value in differentiating ALS from clinically relevant ALS mimics. Plasma NFL levels can be used to differentiate between clinical and genetic ALS subgroups.

sted, utgiver, år, opplag, sider
Nature Publishing Group, 2021
HSV kategori
Identifikatorer
urn:nbn:se:umu:diva-189808 (URN)10.1038/s41598-021-01499-6 (DOI)000717747400105 ()2-s2.0-85118949353 (Scopus ID)
Tilgjengelig fra: 2021-11-22 Laget: 2021-11-22 Sist oppdatert: 2024-03-25bibliografisk kontrollert
Olesen, M. N., Wuolikainen, A., Nilsson, A. C., Wirenfeldt, M., Forsberg, K., Madsen, J. S., . . . Asgari, N. (2020). Inflammatory profiles relate to survival in subtypes of amyotrophic lateral sclerosis. Neurology: Neuroimmunology & Neuroinflammation, 7(3), Article ID e697.
Åpne denne publikasjonen i ny fane eller vindu >>Inflammatory profiles relate to survival in subtypes of amyotrophic lateral sclerosis
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2020 (engelsk)Inngår i: Neurology: Neuroimmunology & Neuroinflammation, E-ISSN 2332-7812, Vol. 7, nr 3, artikkel-id e697Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Objective: To investigate inflammatory cytokines in patients with motor neuron disease (MND) evaluating the putative contribution of amyotrophic lateral sclerosis (ALS)-causing gene variants.

Methods: This study is a retrospective case series with prospective follow-up (1994–2016) of 248 patients with MND, of whom 164 had ALS who were screened for mutations in the genes for SOD1 and C9orf72. Paired CSF and plasma were collected at the diagnostic evaluation before treatment. A panel of cytokines were measured blindly via digital ELISA on the Simoa platform.

Results: Time from disease onset to death was longer for patients with ALS-causing SOD1 mutations (mSOD1, n = 24) than those with C9orf72 hexanucleotide repeat expansion (C9orf72HRE) ALS (n = 19; q = 0.001) and other ALS (OALS) (n = 119; q = 0.0008). Patients with OALS had higher CSF tumor necrosis factor alpha (TNF-α) compared with those with C9orf72HRE ALS (q = 0.014). Patients with C9orf72HRE ALS had higher CSF interferon alpha compared with those with OALS and mSOD1 ALS (q = 0.042 and q = 0.042). In patients with ALS, the survival was negatively correlated with plasma interleukin (IL) 10 (hazard ratio [HR] 1.17, 95% CI 1.05–1.30). Plasma TNF-α, IL-10, and TNF-related apoptosis-inducing ligand (TRAIL) (HR 1.01 [1.00–1.02], 1.15 [1.02–1.30], and 1.01 [1.00–1.01], respectively) of patients with OALS, plasma IL-1β (HR 5.90 [1.27–27.5]) of patients with C9orf72HRE ALS, and CSF TRAIL (10.5 [1.12–98.6]) of patients with mSOD1 ALS all correlated negatively with survival.

Conclusions: Differences in survival times in ALS subtypes were correlated with cytokine levels, suggesting specific immune responses related to ALS genetic variants.

sted, utgiver, år, opplag, sider
Wolters Kluwer, 2020
HSV kategori
Identifikatorer
urn:nbn:se:umu:diva-173325 (URN)10.1212/NXI.0000000000000697 (DOI)000541150500019 ()32123048 (PubMedID)2-s2.0-85080935579 (Scopus ID)
Forskningsfinansiär
Swedish Research Council, 2012-0262Swedish Research Council, 2012-0305Swedish Research Council, 2013-0279Swedish Research Council, 2016-0303Swedish Research Council, 2012-3167Swedish Research Council, 2017-03100Knut and Alice Wallenberg Foundation, 2012.0091Knut and Alice Wallenberg Foundation, 2014.0305The Kempe FoundationsTorsten Söderbergs stiftelseRagnar Söderbergs stiftelseVästerbotten County Council, 56103-7002829
Tilgjengelig fra: 2020-07-02 Laget: 2020-07-02 Sist oppdatert: 2023-09-07bibliografisk kontrollert
Lakso, H.-Å., Wuolikainen, A., Sundkvist, A., Johansson, I. & Marklund, S. L. (2019). Long-term stability of the alcohol consumption biomarker phosphatidylethanol in erythrocytes at-80 degrees C. Clinical Mass Spectrometry, 11, 37-41
Åpne denne publikasjonen i ny fane eller vindu >>Long-term stability of the alcohol consumption biomarker phosphatidylethanol in erythrocytes at-80 degrees C
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2019 (engelsk)Inngår i: Clinical Mass Spectrometry, ISSN 2213-8005, E-ISSN 2376-9998, Vol. 11, s. 37-41Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Phosphatidylethanol (PEth) is a recently introduced biomarker with high specificity, high sensitivity, and response correlating with alcohol consumption. It has the potential to be a valuable biomarker in population studies on the health effects of alcohol, however its stability in long-term stored blood is not known. We used LCMS/MS to assess the stability of PEth-16:0/18:1 in blood samples (packed erythrocytes) that were stored between 1 and 19 years at -80 degrees C in a biobank from a large population survey. The participants answered a lifestyle questionnaire that included questions on alcohol consumption. For analysis, we selected blood samples from seven homogenous ethanol consumption cohorts collected at intervals from 1997 to 2015. Despite the narrow stated alcohol consumption range, 10-15 g/day, there were large differences in PEth values between individuals in the cohorts, from below the limit of detection of 0.005 mu mol/L to 1.40 mu mol/L. The median was 0.08 mu mol/L. Neither generalized linear modeling, nor principal component analysis revealed a statistically significant association between time of storage and PEth levels. The PEth results indicate that the participants had, on average, under-reported their alcohol consumption several-fold. The findings suggest that PEth in blood has a sufficient long-term stability for use as an alcohol biomarker in prospective case-control studies. Analysis of blood stored in biobanks could significantly improve the validity of assessments exploring the health effects of alcohol.

sted, utgiver, år, opplag, sider
Elsevier, 2019
Emneord
Biobank, Stability, Ethanol, Health effects, Population studies
HSV kategori
Identifikatorer
urn:nbn:se:umu:diva-165732 (URN)10.1016/j.clinms.2018.12.002 (DOI)000496420500006 ()2-s2.0-85059236780 (Scopus ID)
Forskningsfinansiär
Swedish Research CouncilVästerbotten County Council
Tilgjengelig fra: 2019-12-10 Laget: 2019-12-10 Sist oppdatert: 2023-03-24bibliografisk kontrollert
Zhu, S., Wuolikainen, A., Wu, J., Öhman, A., Wingsle, G., Moritz, T., . . . Trupp, M. (2019). Targeted Multiple Reaction Monitoring Analysis of CSF Identifies UCHL1 and GPNMB as Candidate Biomarkers for ALS. Journal of Molecular Neuroscience, 69(4), 643-657
Åpne denne publikasjonen i ny fane eller vindu >>Targeted Multiple Reaction Monitoring Analysis of CSF Identifies UCHL1 and GPNMB as Candidate Biomarkers for ALS
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2019 (engelsk)Inngår i: Journal of Molecular Neuroscience, ISSN 0895-8696, E-ISSN 1559-1166, Vol. 69, nr 4, s. 643-657Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The neurodegenerative diseases amyotrophic lateral sclerosis (ALS) and Parkinson's disease (PD) share some common molecular deficits including disruption of protein homeostasis leading to disease-specific protein aggregation. While insoluble protein aggregates are the defining pathological confirmation of diagnosis, patient stratification based on early molecular etiologies may identify distinct subgroups within a clinical diagnosis that would respond differently in therapeutic development programs. We are developing targeted multiple reaction monitoring (MRM) mass spectrometry methods to rigorously quantify CSF proteins from known disease genes involved in lysosomal, ubiquitin-proteasomal, and autophagy pathways. Analysis of CSF from 21 PD, 21 ALS, and 25 control patients, rigorously matched for gender, age, and age of sample, revealed significant changes in peptide levels between PD, ALS, and control. In patients with PD, levels of two peptides for chromogranin B (CHGB, secretogranin 1) were significantly reduced. In CSF of patients with ALS, levels of two peptides from ubiquitin carboxy-terminal hydrolase like protein 1 (UCHL1) and one peptide each for glycoprotein non-metastatic melanoma protein B (GPNMB) and cathepsin D (CTSD) were all increased. Analysis of patients with ALS separated into two groups based on length of survival after CSF sampling revealed that the increases in GPNMB and UCHL1 were specific for short-lived ALS patients. While analysis of additional cohorts is required to validate these candidate biomarkers, this study suggests methods for stratification of ALS patients for clinical trials and identifies targets for drug efficacy measurements during therapeutic development.

sted, utgiver, år, opplag, sider
Springer, 2019
Emneord
CSF biomarker, Proteomics, Parkinson's disease, ALS, Protein homeostasis
HSV kategori
Identifikatorer
urn:nbn:se:umu:diva-165738 (URN)10.1007/s12031-019-01411-y (DOI)000495982700002 ()31721001 (PubMedID)2-s2.0-85075078414 (Scopus ID)
Forskningsfinansiär
The Kempe FoundationsSwedish Research Council
Tilgjengelig fra: 2019-12-10 Laget: 2019-12-10 Sist oppdatert: 2023-03-24bibliografisk kontrollert
Nordin, A., Akimoto, C., Wuolikainen, A., Alstermark, H., Forsberg, K., Baumann, P., . . . Andersen, P. M. (2017). Sequence variations in C9orf72 downstream of the hexanucleotide repeat region and its effect on repeat-primed PCR interpretation: a large multinational screening study. Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration, 18(3-4), 256-264
Åpne denne publikasjonen i ny fane eller vindu >>Sequence variations in C9orf72 downstream of the hexanucleotide repeat region and its effect on repeat-primed PCR interpretation: a large multinational screening study
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2017 (engelsk)Inngår i: Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration, ISSN 2167-8421, E-ISSN 2167-9223, Vol. 18, nr 3-4, s. 256-264Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

A large GGGGCC-repeat expansion mutation (HREM) in C9orf72 is the most common known cause of ALS and FTD in European populations. Sequence variations immediately downstream of the HREM region have previously been observed and have been suggested to be one reason for difficulties in interpreting RP-PCR data. Our objective was to determine the properties of these sequence variations with regard to prevalence, the range of variation, and effect on disease prognosis. We screened a multi-national cohort (n = 6981) for the HREM and samples with deviant RP-PCR curves were identified. The deviant samples were subsequently sequenced to determine sequence alteration. Our results show that in the USA and European cohorts (n = 6508) 10.7% carried the HREM and 3% had a sequence variant, while no HREM or sequence variants were observed in the Japanese cohort (n = 473). Sequence variations were more common on HREM alleles; however, certain population specific variants were associated with a non-expanded allele. In conclusion, we identified 38 different sequence variants, most located within the first 50 bp downstream of the HREM region. Furthermore, the presence of an HREM was found to be coupled to a lower age of onset and a shorter disease survival, while sequence variation did not have any correlation with these parameters.

Emneord
ALS, C9orf72, FTD, RP-PCR interpretation, variants
HSV kategori
Identifikatorer
urn:nbn:se:umu:diva-134981 (URN)10.1080/21678421.2016.1262423 (DOI)000400792800012 ()27936955 (PubMedID)2-s2.0-85003794482 (Scopus ID)
Tilgjengelig fra: 2017-05-15 Laget: 2017-05-15 Sist oppdatert: 2023-03-23bibliografisk kontrollert
Ingre, C., Wuolikainen, A., Marklund, S. L., Birve, A., Press, R. & Andersen, P. M. (2016). A 50bp deletion in the SOD1 promoter lowers enzyme expression but is not associated with ALS in Sweden. Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration, 17(5-6), 452-457
Åpne denne publikasjonen i ny fane eller vindu >>A 50bp deletion in the SOD1 promoter lowers enzyme expression but is not associated with ALS in Sweden
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2016 (engelsk)Inngår i: Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration, ISSN 2167-8421, E-ISSN 2167-9223, Vol. 17, nr 5-6, s. 452-457Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Mutations in the superoxide dismutase (SOD1) gene have been linked to amyotrophic lateral sclerosis (ALS). A 50 base pair (bp) deletion of SOD1 has been suggested to reduce transcription and to be associated with later disease onset in ALS. This study was aimed to reveal if the 50bp deletion influenced SOD1 enzymatic activity, occurrence and phenotype of the disease in a Swedish ALS/control cohort. Blood samples from 512 Swedish ALS patients and 354 Swedish controls without coding SOD1 mutations were analysed for the 50bp deletion allele. The enzymatic activity of SOD1 in erythrocytes was analysed and genotype-phenotype correlations were assessed. Results demonstrated that the genotype frequencies of the 50bp deletion were all found to be in Hardy-Weinberg equilibrium. No significant differences were found for age of onset, disease duration or site of onset. SOD1 enzymatic activity showed a statistically significant decreasing trend in the control group, in which the allele was associated with a 5% reduction in SOD1 activity. The results suggest that the 50bp deletion has a moderate reducing effect on SOD1 synthesis. No modulating effects, however, were found on ALS onset, phenotype and survival in the Swedish population.

Emneord
ALS, SOD1, promoter, deletion, age of onset, disease duration
HSV kategori
Identifikatorer
urn:nbn:se:umu:diva-127984 (URN)10.3109/21678421.2016.1159223 (DOI)000381024500019 ()27002425 (PubMedID)2-s2.0-84961391622 (Scopus ID)
Tilgjengelig fra: 2016-12-05 Laget: 2016-11-21 Sist oppdatert: 2023-03-24bibliografisk kontrollert
Bergman, J., Dring, A., Wuolikainen, A., Gilthorpe, J., Bergenheim, T. & Svenningsson, A. (2016). Cytokine levels in interstitial brain fluid in progressive multiple sclerosis measured via intracerebral microdialysis. Paper presented at 32nd Congress of the European-Committee-for-Treatment-and-Research-in-Multiple-Sclerosis (ECTRIMS), SEP 14-17, 2016, London, ENGLAND. Multiple Sclerosis Journal, 22, 511-511
Åpne denne publikasjonen i ny fane eller vindu >>Cytokine levels in interstitial brain fluid in progressive multiple sclerosis measured via intracerebral microdialysis
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2016 (engelsk)Inngår i: Multiple Sclerosis Journal, ISSN 1352-4585, E-ISSN 1477-0970, Vol. 22, s. 511-511Artikkel i tidsskrift, Meeting abstract (Fagfellevurdert) Published
sted, utgiver, år, opplag, sider
SAGE PUBLICATIONS LTD, 2016
HSV kategori
Identifikatorer
urn:nbn:se:umu:diva-132859 (URN)000383267202206 ()
Konferanse
32nd Congress of the European-Committee-for-Treatment-and-Research-in-Multiple-Sclerosis (ECTRIMS), SEP 14-17, 2016, London, ENGLAND
Merknad

Supplement 3, Meeting Abstract P1005

Tilgjengelig fra: 2017-03-23 Laget: 2017-03-23 Sist oppdatert: 2022-09-15bibliografisk kontrollert
Wuolikainen, A., Jonsson, P., Ahnlund, M., Antti, H., Marklund, S. L., Moritz, T., . . . Trupp, M. (2016). Multi-platform mass spectrometry analysis of the CSF and plasma metabolomes of rigorously matched amyotrophic lateral sclerosis, Parkinson's disease and control subjects. Molecular Biosystems, 12(4), 1287-1298
Åpne denne publikasjonen i ny fane eller vindu >>Multi-platform mass spectrometry analysis of the CSF and plasma metabolomes of rigorously matched amyotrophic lateral sclerosis, Parkinson's disease and control subjects
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2016 (engelsk)Inngår i: Molecular Biosystems, ISSN 1742-206X, E-ISSN 1742-2051, Vol. 12, nr 4, s. 1287-1298Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Amyotrophic lateral sclerosis (ALS) and Parkinson's disease (PD) are protein-aggregation diseases that lack clear molecular etiologies. Biomarkers could aid in diagnosis, prognosis, planning of care, drug target identification and stratification of patients into clinical trials. We sought to characterize shared and unique metabolite perturbations between ALS and PD and matched controls selected from patients with other diagnoses, including differential diagnoses to ALS or PD that visited our clinic for a lumbar puncture. Cerebrospinal fluid (CSF) and plasma from rigorously age-, sex- and sampling-date matched patients were analyzed on multiple platforms using gas chromatography (GC) and liquid chromatography (LC)-mass spectrometry (MS). We applied constrained randomization of run orders and orthogonal partial least squares projection to latent structure-effect projections (OPLS-EP) to capitalize upon the study design. The combined platforms identified 144 CSF and 196 plasma metabolites with diverse molecular properties. Creatine was found to be increased and creatinine decreased in CSF of ALS patients compared to matched controls. Glucose was increased in CSF of ALS patients and alpha-hydroxybutyrate was increased in CSF and plasma of ALS patients compared to matched controls. Leucine, isoleucine and ketoleucine were increased in CSF of both ALS and PD. Together, these studies, in conjunction with earlier studies, suggest alterations in energy utilization pathways and have identified and further validated perturbed metabolites to be used in panels of biomarkers for the diagnosis of ALS and PD.

HSV kategori
Identifikatorer
urn:nbn:se:umu:diva-119312 (URN)10.1039/c5mb00711a (DOI)000372612600023 ()26883206 (PubMedID)2-s2.0-84962052942 (Scopus ID)
Tilgjengelig fra: 2016-04-17 Laget: 2016-04-15 Sist oppdatert: 2023-03-24bibliografisk kontrollert
Wu, J., Wuolikainen, A., Trupp, M., Jonsson, P., Marklund, S. L., Andersen, P. M., . . . Öhman, A. (2016). NMR analysis of the CSF and plasma metabolome of rigorously matched amyotrophic lateral sclerosis, Parkinson's disease and control subjects. Metabolomics, 12(6), Article ID 101.
Åpne denne publikasjonen i ny fane eller vindu >>NMR analysis of the CSF and plasma metabolome of rigorously matched amyotrophic lateral sclerosis, Parkinson's disease and control subjects
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2016 (engelsk)Inngår i: Metabolomics, ISSN 1573-3882, E-ISSN 1573-3890, Vol. 12, nr 6, artikkel-id 101Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Introduction: Amyotrophic lateral sclerosis (ALS) and Parkinson’s disease (PD) are two severe neurodegenerative disorders for which the disease mechanisms are poorly understood and reliable biomarkers are absent.

Objectives: To identify metabolite biomarkers for ALS and PD, and to gain insights into which metabolic pathways are involved in disease.

Methods: Nuclear magnetic resonance (NMR) metabolomics was utilized to characterize the metabolite profiles of cerebrospinal fluid (CSF) and plasma from individuals in three age, gender, and sampling-date matched groups, comprising 22 ALS, 22 PD and 28 control subjects.

Results: Multivariate analysis of NMR data generated robust discriminatory models for separation of ALS from control subjects. ALS patients showed increased concentrations of several metabolites in both CSF and plasma, these are alanine (CSF fold change = 1.22, p = 0.005), creatine (CSF-fc = 1.17, p = 0.001), glucose (CSF-fc = 1.11, p = 0.036), isoleucine (CSF-fc = 1.24, p = 0.002), and valine (CSF-fc = 1.17, p = 0.014). Additional metabolites in CSF (creatinine, dimethylamine and lactic acid) and plasma (acetic acid, glutamic acid, histidine, leucine, pyruvate and tyrosine) were also important for this discrimination. Similarly, panels of CSF-metabolites that discriminate PD from ALS and control subjects were identified.

Conclusions: The results for the ALS patients suggest an affected creatine/creatinine pathway and an altered branched chain amino acid (BCAA) metabolism, and suggest links to glucose and energy metabolism. Putative metabolic markers specific for ALS (e.g. creatinine and lactic acid) and PD (e.g. 3-hydroxyisovaleric acid and mannose) were identified, while several (e.g. creatine and BCAAs) were shared between ALS and PD, suggesting some overlap in metabolic alterations in these disorders.

Emneord
Amyotrophic lateral sclerosis (ALS), Parkinson's disease (PD), NMR metabolomics, Biomarker, rebrospinal fluid (CSF), Plasma
HSV kategori
Identifikatorer
urn:nbn:se:umu:diva-124194 (URN)10.1007/s11306-016-1041-6 (DOI)000378752900006 ()2-s2.0-84964981513 (Scopus ID)
Tilgjengelig fra: 2016-08-04 Laget: 2016-07-28 Sist oppdatert: 2023-03-24bibliografisk kontrollert
Jonsson, P., Wuolikainen, A., Thysell, E., Chorell, E., Stattin, P., Wikström, P. & Antti, H. (2015). Constrained randomization and multivariate effect projections improve information extraction and biomarker pattern discovery in metabolomics studies involving dependent samples. Metabolomics, 11(6), 1667-1678
Åpne denne publikasjonen i ny fane eller vindu >>Constrained randomization and multivariate effect projections improve information extraction and biomarker pattern discovery in metabolomics studies involving dependent samples
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2015 (engelsk)Inngår i: Metabolomics, ISSN 1573-3882, E-ISSN 1573-3890, Vol. 11, nr 6, s. 1667-1678Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Analytical drift is a major source of bias in mass spectrometry based metabolomics confounding interpretation and biomarker detection. So far, standard protocols for sample and data analysis have not been able to fully resolve this. We present a combined approach for minimizing the influence of analytical drift on multivariate comparisons of matched or dependent samples in mass spectrometry based metabolomics studies. The approach is building on a randomization procedure for sample run order, constrained to independent randomizations between and within dependent sample pairs (e.g. pre/post intervention). This is followed by a novel multivariate statistical analysis strategy allowing paired or dependent analyses of individual effects named OPLS-effect projections (OPLS-EP). We show, using simulated data that OPLS-EP gives improved interpretation over existing methods and that constrained randomization of sample run order in combination with an appropriate dependent statistical test increase the accuracy and sensitivity and decrease the false omission rate in biomarker detection. We verify these findings and prove the strength of the suggested approach in a clinical data set consisting of LC/MS data of blood plasma samples from patients before and after radical prostatectomy. Here OPLS-EP compared to traditional (independent) OPLS-discriminant analysis (OPLS-DA) on constrained randomized data gives a less complex model (3 versus 5 components) as well a higher predictive ability (Q2 = 0.80 versus Q2 = 0.55). We explain this by showing that paired statistical analysis detects 37 unique significant metabolites that were masked for the independent test due to bias, including analytical drift and inter-individual variation.

sted, utgiver, år, opplag, sider
Springer, 2015
Emneord
Metabolomics, Chemometrics, Dependent samples, Analytical drift, Run order design, Effect projections
HSV kategori
Identifikatorer
urn:nbn:se:umu:diva-111340 (URN)10.1007/s11306-015-0818-3 (DOI)000363040600017 ()2-s2.0-84944279233 (Scopus ID)
Tilgjengelig fra: 2015-11-13 Laget: 2015-11-13 Sist oppdatert: 2023-03-24bibliografisk kontrollert
Organisasjoner