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Chen, Jialin
Publikationer (10 of 12) Visa alla publikationer
Chen, J., Backman, L. J., Zhang, W., Ling, C. & Danielson, P. (2020). Regulation of Keratocyte Phenotype and Cell Behavior by Substrate Stiffness. ACS Biomaterials Science & Engineering, 6(9), 5162-5171
Öppna denna publikation i ny flik eller fönster >>Regulation of Keratocyte Phenotype and Cell Behavior by Substrate Stiffness
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2020 (Engelska)Ingår i: ACS Biomaterials Science & Engineering, E-ISSN 2373-9878, Vol. 6, nr 9, s. 5162-5171Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Corneal tissue engineering is an alternative way to solve the problem of lack of corneal donor tissue in corneal transplantation. Keratocytes with a normal phenotype and function in tissue-engineered cornea would be critical for corneal regeneration. Although the role of extracellular/substrate material stiffness is well-known for the regulation of the cell phenotype and cell behavior in many different cell types, its effects in keratocyte culture have not yet been thoroughly studied. This project studied the effect of substrate stiffness on the keratocyte phenotype marker expression and typical cell behavior (cell adhesion, proliferation, and migration), and the possible mechanisms involved. Human primary keratocytes were cultured on tissue culture plastic (TCP, similar to 10(6) kPa) or on plates with the stiffness equivalent of physiological human corneal stroma (25 kPa) or vitreous body (1 kPa). The expression of keratocyte phenotype markers, cell adhesion, proliferation, and migration were compared. The results showed that the stiffness of the substrate material regulates the phenotype marker expression and cell behavior of cultured keratocytes. Physiological corneal stiffness (25 kPa) superiorly preserved the cell phenotype when compared to the TCP and 1 kPa group. Keratocytes had a larger cell area when cultured on 25 kPa plates as compared to on TCP. Treatment of cells with NSC 23766 (Rac1 inhibitor) mimicked the response in the cell phenotype and behavior seen in the transition from soft materials to stiff materials, including the cytoskeletal structure, expression of keratocyte phenotype markers, and cell behavior. In conclusion, this study shows that substrate stiffness regulates the cell phenotype marker expression and cell behavior of keratocytes by Rac1-mediated cytoskeletal reorganization. This knowledge contributes to the development of corneal tissue engineering.

Ort, förlag, år, upplaga, sidor
American Chemical Society (ACS), 2020
Nyckelord
keratocytes, stiffness, phenotype, cell behavior, cytoskeletal reorganization, Rac1
Nationell ämneskategori
Oftalmologi
Identifikatorer
urn:nbn:se:umu:diva-176078 (URN)10.1021/acsbiomaterials.0c00510 (DOI)000572822300037 ()2-s2.0-85092544653 (Scopus ID)
Tillgänglig från: 2020-10-23 Skapad: 2020-10-23 Senast uppdaterad: 2023-03-23Bibliografiskt granskad
Zhang, W., Chen, J., Qu, M., Backman, L. J., Zhang, A., Liu, H., . . . Danielson, P. (2020). Sustained Release of TPCA-1 from Silk Fibroin Hydrogels Preserves Keratocyte Phenotype and Promotes Corneal Regeneration by Inhibiting Interleukin-1β Signaling. Advanced Healthcare Materials, 9(17), Article ID 2000591.
Öppna denna publikation i ny flik eller fönster >>Sustained Release of TPCA-1 from Silk Fibroin Hydrogels Preserves Keratocyte Phenotype and Promotes Corneal Regeneration by Inhibiting Interleukin-1β Signaling
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2020 (Engelska)Ingår i: Advanced Healthcare Materials, ISSN 2192-2640, E-ISSN 2192-2659, Vol. 9, nr 17, artikel-id 2000591Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Corneal injury due to ocular trauma or infection is one of the most challenging vision impairing pathologies that exists. Many studies focus on the pro-inflammatory and pro-angiogenic effects of interleukin-1 beta(IL-1 beta) on corneal wound healing. However, the effect of IL-1 beta on keratocyte phenotype and corneal repair, as well as the underlying mechanisms, is not clear. This study reports, for the first time, that IL-1 beta induces phenotype changes of keratocytes in vitro, by significantly down-regulating the gene and protein expression levels of keratocyte markers (Keratocan, Lumican, Aldh3a1 and CD34). Furthermore, it is found that the NF-kappa B pathway is involved in the IL-1 beta-induced changes of keratocyte phenotype, and that the selective IKK beta inhibitor TPCA-1, which inhibits NF-kappa B, can preserve keratocyte phenotype under IL-1 beta simulated pathological conditions in vitro. By using a murine model of corneal injury, it is shown that sustained release of TPCA-1 from degradable silk fibroin hydrogels accelerates corneal wound healing, improves corneal transparency, enhances the expression of keratocyte markers, and supports the regeneration of well-organized epithelium and stroma. These findings provide insights not only into the pathophysiological mechanisms of corneal wound healing, but also into the potential development of new treatments for patients with corneal injuries.

Ort, förlag, år, upplaga, sidor
Wiley-VCH Verlagsgesellschaft, 2020
Nyckelord
corneal regeneration, interleukin-1 beta, keratocyte, NF-kappa B signaling, silk fibroin
Nationell ämneskategori
Oftalmologi
Identifikatorer
urn:nbn:se:umu:diva-174033 (URN)10.1002/adhm.202000591 (DOI)000554483600001 ()32743953 (PubMedID)2-s2.0-85088834694 (Scopus ID)
Tillgänglig från: 2020-08-14 Skapad: 2020-08-14 Senast uppdaterad: 2023-03-24Bibliografiskt granskad
Chen, J., Zhang, W., Backman, L. J., Kelk, P. & Danielson, P. (2018). Mechanical stress potentiates the differentiation of periodontal ligament stem cells into keratocytes. British Journal of Ophthalmology, 102(4), 562-569
Öppna denna publikation i ny flik eller fönster >>Mechanical stress potentiates the differentiation of periodontal ligament stem cells into keratocytes
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2018 (Engelska)Ingår i: British Journal of Ophthalmology, ISSN 0007-1161, E-ISSN 1468-2079, Vol. 102, nr 4, s. 562-569Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Aims To explore the role of corneal-shaped static mechanical strain on the differentiation of human periodontal ligament stem cells (PDLSCs) into keratocytes and the possible synergistic effects of mechanics and inducing medium. Methods PDLSCs were exposed to 3% static dome-shaped mechanical strain in a Flexcell Tension System for 3 days and 7 days. Keratocyte phenotype was determined by gene expression of keratocyte markers. Keratocyte differentiation (inducing) medium was introduced in the Flexcell system, either continuously or intermittently combined with mechanical stimulation. The synergistic effects of mechanics and inducing medium on keratocyte differentiation was evaluated by gene and protein expression of keratocyte markers. Finally, a multilamellar cell sheet was assembled by seeding PDLSCs on a collagen membrane and inducing keratocyte differentiation. The transparency of the cell sheet was assessed, and typical markers of native human corneal stroma were evaluated by immunofluorescence staining. Results Dome-shaped mechanical stimulation promoted PDLSCs to differentiate into keratocytes, as shown by the upregulation of ALDH3A1, CD34, LUM, COL I and COL V. The expression of integrins were also upregulated after mechanical stimulation, including integrin alpha 1, alpha 2, beta 1 and non-muscle myosin II B. A synergistic effect of mechanics and inducing medium was found on keratocyte differentiation. The cell sheets were assembled under the treatment of mechanics and inducing medium simultaneously. The cell sheets were transparent, multilamellar and expressed typical markers of corneal stroma. Conclusion Dome-shaped mechanical stimulation promotes differentiation of PDLSCs into keratocytes and has synergistic effects with inducing medium. Multilamellar cell sheets that resemble native human corneal stroma show potential for future clinical applications.

Ort, förlag, år, upplaga, sidor
BMJ Publishing Group Ltd, 2018
Nyckelord
PDLSCs, corneal stroma, mechanics, inducing medium, differentiation, cell-sheet
Nationell ämneskategori
Oftalmologi
Identifikatorer
urn:nbn:se:umu:diva-147334 (URN)10.1136/bjophthalmol-2017-311150 (DOI)000429732500026 ()29306866 (PubMedID)2-s2.0-85044936731 (Scopus ID)
Tillgänglig från: 2018-05-17 Skapad: 2018-05-17 Senast uppdaterad: 2023-03-24Bibliografiskt granskad
El-Habta, R., Chen, J., Pingel, J. & Backman, L. J. (2018). Tendinosis-like changes in denervated rat Achilles tendon. BMC Musculoskeletal Disorders, 19, Article ID 426.
Öppna denna publikation i ny flik eller fönster >>Tendinosis-like changes in denervated rat Achilles tendon
2018 (Engelska)Ingår i: BMC Musculoskeletal Disorders, E-ISSN 1471-2474, Vol. 19, artikel-id 426Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background: Tendon disorders are common and lead to significant disability and pain. Our knowledge of the ‘tennis elbow’, the ‘jumpers knee’, and Achilles tendinosis has increased over the years, but changes in denervated tendons is yet to be described in detail. The aim of the present study was to investigate the morphological and biochemical changes in tendon tissue following two weeks of denervation using a unilateral sciatic nerve transection model in rat Achilles tendons.

Methods: Tendons were compared with respect to cell number, nuclear roundness, and fiber structure. The non-denervated contralateral tendon served as a control. Also, the expression of neuromodulators such as substance P and its preferred receptor neurokinin-1 receptor, NK-1R, was evaluated using real-time qRT-PCR.

Results: Our results showed that denervated tendons expressed morphological changes such as hypercellularity; disfigured cells; disorganization of the collagen network; increased production of type III collagen; and increased expression of NK-1R.

Conclusion: Taken together these data provide new insights into the histopathology of denervated tendons showing that denervation causes somewhat similar changes in the Achilles tendon as does tendinosis in rats.

Ort, förlag, år, upplaga, sidor
BMC, 2018
Nyckelord
Collagen, Denervation, Rat, Substance P, Tendinosis
Nationell ämneskategori
Ortopedi
Identifikatorer
urn:nbn:se:umu:diva-154940 (URN)10.1186/s12891-018-2353-7 (DOI)000451699800006 ()30497469 (PubMedID)2-s2.0-85057541287 (Scopus ID)
Tillgänglig från: 2019-01-08 Skapad: 2019-01-08 Senast uppdaterad: 2024-01-17Bibliografiskt granskad
Chen, J., Lan, J., Liu, D., Backman, L. J., Zhang, W., Zhou, Q. & Danielson, P. (2017). Ascorbic Acid Promotes the Stemness of Corneal Epithelial Stem/Progenitor Cells and Accelerates Epithelial Wound Healing in the Cornea. Stem Cells Translational Medicine, 6(5), 1356-1365
Öppna denna publikation i ny flik eller fönster >>Ascorbic Acid Promotes the Stemness of Corneal Epithelial Stem/Progenitor Cells and Accelerates Epithelial Wound Healing in the Cornea
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2017 (Engelska)Ingår i: Stem Cells Translational Medicine, ISSN 2157-6564, E-ISSN 2157-6580, Vol. 6, nr 5, s. 1356-1365Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

High concentration of ascorbic acid (vitamin C) has been found in corneal epithelium of various species. However, the specific functions and mechanisms of ascorbic acid in the repair of corneal epithelium are not clear. In this study, it was found that ascorbic acid accelerates corneal epithelial wound healing in vivo in mouse. In addition, ascorbic acid enhanced the stemness of cultured mouse corneal epithelial stem/progenitor cells (TKE2) in vitro, as shown by elevated clone formation ability and increased expression of stemness markers (especially p63 and SOX2). The contribution of ascorbic acid on the stemness enhancement was not dependent on the promotion of Akt phosphorylation, as concluded by using Akt inhibitor, nor was the stemness found to be dependent on the regulation of oxidative stress, as seen by the use of two other antioxidants (GMEE and NAC). However, ascorbic acid was found to promote extracellular matrix (ECM) production, and by using two collagen synthesis inhibitors (AzC and CIS), the increased expression of p63 and SOX2 by ascorbic acid was decreased by around 50%, showing that the increased stemness by ascorbic acid can be attributed to its regulation of ECM components. Moreover, the expression of p63 and SOX2 was elevated when TKE2 cells were cultured on collagen I coated plates, a situation that mimics the in vivo situation as collagen I is the main component in the corneal stroma. This study shows direct therapeutic benefits of ascorbic acid on corneal epithelial wound healing and provides new insights into the mechanisms involved.

Ort, förlag, år, upplaga, sidor
WILEY, 2017
Nyckelord
Stem/progenitor cell, Colony formation, Proliferation, Microenvironment, Stem cell- croenvironment interactions, Tissue regeneration
Nationell ämneskategori
Cell- och molekylärbiologi
Identifikatorer
urn:nbn:se:umu:diva-136201 (URN)10.1002/sctm.16-0441 (DOI)000400596200009 ()28276172 (PubMedID)2-s2.0-85019404553 (Scopus ID)
Tillgänglig från: 2017-07-03 Skapad: 2017-07-03 Senast uppdaterad: 2023-03-24Bibliografiskt granskad
Chen, J., Zhang, E., Zhang, W., Liu, Z., Lu, P., Zhu, T., . . . Ouyang, H. (2017). Fos Promotes Early Stage Teno-Lineage Differentiation of Tendon Stem/Progenitor Cells in Tendon. Stem Cells Translational Medicine, 6(11), 2009-2019
Öppna denna publikation i ny flik eller fönster >>Fos Promotes Early Stage Teno-Lineage Differentiation of Tendon Stem/Progenitor Cells in Tendon
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2017 (Engelska)Ingår i: Stem Cells Translational Medicine, ISSN 2157-6564, E-ISSN 2157-6580, Vol. 6, nr 11, s. 2009-2019Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Stem cells have been widely used in tendon tissue engineering. The lack of refined and controlled differentiation strategy hampers the tendon repair and regeneration. This study aimed to find new effective differentiation factors for stepwise tenogenic differentiation. By microarray screening, the transcript factor Fos was found to be expressed in significantly higher amounts in postnatal Achilles tendon tissue derived from 1 day as compared with 7-days-old rats. It was further confirmed that expression of Fos decreased with time in postnatal rat Achilles tendon, which was accompanied with the decreased expression of multiply tendon markers. The expression of Fos also declined during regular in vitro cell culture, which corresponded to the loss of tendon phenotype. In a cell-sheet and a three-dimensional cell culture model, the expression of Fos was upregulated as compared with in regular cell culture, together with the recovery of tendon phenotype. In addition, significant higher expression of tendon markers was found in Fos-overexpressed tendon stem/progenitor cells (TSPCs), and Fos knock-down gave opposite results. In situ rat tendon repair experiments found more normal tendon-like tissue formed and higher tendon markers expression at 4 weeks postimplantation of Fos-overexpressed TSPCs derived nonscaffold engineering tendon (cell-sheet), as compared with the control group. This study identifies Fos as a new marker and functional driver in the early stage teno-lineage differentiation of tendon, which paves the way for effective stepwise tendon differentiation and future tendon regeneration.

Ort, förlag, år, upplaga, sidor
John Wiley & Sons, 2017
Nationell ämneskategori
Cell- och molekylärbiologi
Identifikatorer
urn:nbn:se:umu:diva-143601 (URN)10.1002/sctm.15-0146 (DOI)000416214500007 ()29024580 (PubMedID)2-s2.0-85032499537 (Scopus ID)
Tillgänglig från: 2018-01-04 Skapad: 2018-01-04 Senast uppdaterad: 2023-03-23Bibliografiskt granskad
Spang, C., Backman, L. J., Le Roux, S., Chen, J. & Danielson, P. (2017). Glutamate signaling through the NMDA receptor reduces the expression of scleraxis in plantaris tendon derived cells. BMC Musculoskeletal Disorders, 18, Article ID 218.
Öppna denna publikation i ny flik eller fönster >>Glutamate signaling through the NMDA receptor reduces the expression of scleraxis in plantaris tendon derived cells
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2017 (Engelska)Ingår i: BMC Musculoskeletal Disorders, E-ISSN 1471-2474, Vol. 18, artikel-id 218Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background: A body of evidence demonstrating changes to the glutaminergic system in tendinopathy has recently emerged. This hypothesis was further tested by studying the effects of glutamate on the tenocyte phenotype, and the impact of loading and exposure to glucocorticoids on the glutamate signaling machinery.

Methods: Plantaris tendon tissue and cultured plantaris tendon derived cells were immunohisto-/cytochemically stained for glutamate, N-Methyl-D-Aspartate receptor 1 (NMDAR1) and vesicular glutamate transporter 2 (VGluT2). Primary cells were exposed to glutamate or receptor agonist NMDA. Cell death/viability was measured via LDH/MTS assays, and Western blot for cleaved caspase 3 (c-caspase 3) and cleaved poly (ADP-ribose) polymerase (c-PARP). Scleraxis mRNA (Scx)/protein(SCX) were analyzed by qPCR and Western blot, respectively. A FlexCell system was used to apply cyclic strain. The effect of glucocorticoids was studies by adding dexamethasone (Dex). The mRNA of the glutamate synthesizing enzymes Got1 and Gls, and NMDAR1 protein were measured. Levels of free glutamate were determined by a colorimetric assay.

Results: Immunoreactions for glutamate, VGluT2, and NMDAR1 were found in tenocytes and peritendinous cells in tissue sections and in cultured cells. Cell death was induced by high concentrations of glutamate but not by NMDA. Scleraxis mRNA/protein was down-regulated in response to NMDA/glutamate stimulation. Cyclic strain increased, and Dex decreased, Gls and Got1 mRNA expression. Free glutamate levels were lower after Dex exposure.

Conclusions: In conclusion, NMDA receptor stimulation leads to a reduction of scleraxis expression that may be involved in a change of phenotype in tendon cells. Glutamate synthesis is increased in tendon cells in response to strain and decreased by glucocorticoid stimulation. This implies that locally produced glutamate could be involved in the tissue changes observed in tendinopathy.

Nyckelord
Glutamate, NMDAR1, Plantaris tendon, Tendinopathy, Scleraxis
Nationell ämneskategori
Cell- och molekylärbiologi
Identifikatorer
urn:nbn:se:umu:diva-136330 (URN)10.1186/s12891-017-1575-4 (DOI)000402332800006 ()28545490 (PubMedID)2-s2.0-85019852306 (Scopus ID)
Tillgänglig från: 2017-06-21 Skapad: 2017-06-21 Senast uppdaterad: 2024-01-17Bibliografiskt granskad
Chen, J., Zhang, W., Kelk, P., Backman, L. J. & Danielson, P. (2017). Substance P and patterned silk biomaterial stimulate periodontal ligament stem cells to form corneal stroma in a bioengineered three-dimensional model. Stem Cell Research & Therapy, 8, Article ID 260.
Öppna denna publikation i ny flik eller fönster >>Substance P and patterned silk biomaterial stimulate periodontal ligament stem cells to form corneal stroma in a bioengineered three-dimensional model
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2017 (Engelska)Ingår i: Stem Cell Research & Therapy, E-ISSN 1757-6512, Vol. 8, artikel-id 260Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background: We aimed to generate a bioengineered multi-lamellar human corneal stroma tissue in vitro by differentiating periodontal ligament stem cells (PDLSCs) towards keratocytes on an aligned silk membrane.

Methods: Human PDLSCs were isolated and identified. The neuropeptide substance P (SP) was added in keratocyte differentiation medium (KDM) to evaluate its effect on keratocyte differentiation of PDLSCs. PDLSCs were then seeded on patterned silk membrane and cultured with KDM and SP. Cell alignment was evaluated and the expression of extracellular matrix (ECM) components of corneal stroma was detected. Finally, multi-lamellar tissue was constructed in vitro by PDLSCs seeded on patterned silk membranes, which were stacked orthogonally and stimulated by KDM supplemented with SP for 18 days. Sections were prepared and subsequently stained with hematoxylin and eosin or antibodies for immunofluorescence observation of human corneal stroma-related proteins.

Results: SP promoted the expression of corneal stroma-related collagens (collagen types I, III, V, and VI) during the differentiation induced by KDM. Patterned silk membrane guided cell alignment of PDLSCs, and important ECM components of the corneal stroma were shown to be deposited by the cells. The constructed multi-lamellar tissue was found to support cells growing between every two layers and expressing the main type of collagens (collagen types I and V) and proteoglycans (lumican and keratocan) of normal human corneal stroma.

Conclusions: Multi-lamellar human corneal stroma-like tissue can be constructed successfully in vitro by PDLSCs seeded on orthogonally aligned, multi-layered silk membranes with SP supplementation, which shows potential for future corneal tissue engineering.

Ort, förlag, år, upplaga, sidor
BIOMED CENTRAL LTD, 2017
Nyckelord
PDLSCs, Corneal stroma, Substance P, Aligned silk membrane, Differentiation
Nationell ämneskategori
Cell- och molekylärbiologi
Identifikatorer
urn:nbn:se:umu:diva-142239 (URN)10.1186/s13287-017-0715-y (DOI)000415012400001 ()2-s2.0-85034051983 (Scopus ID)
Tillgänglig från: 2017-12-11 Skapad: 2017-12-11 Senast uppdaterad: 2023-03-24Bibliografiskt granskad
Zhang, W., Chen, J., Backman, L. J., Malm, A. D. & Danielson, P. (2017). Surface Topography and Mechanical Strain Promote Keratocyte Phenotype and Extracellular Matrix Formation in a Biomimetic 3D Corneal Model. Advanced Healthcare Materials, 6(5), Article ID 1601238.
Öppna denna publikation i ny flik eller fönster >>Surface Topography and Mechanical Strain Promote Keratocyte Phenotype and Extracellular Matrix Formation in a Biomimetic 3D Corneal Model
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2017 (Engelska)Ingår i: Advanced Healthcare Materials, ISSN 2192-2640, E-ISSN 2192-2659, Vol. 6, nr 5, artikel-id 1601238Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The optimal functionality of the native corneal stroma is mainly dependent on the well-ordered arrangement of extracellular matrix (ECM) and the pressurized structure. In order to develop an in vitro corneal model, it is crucial to mimic the in vivo microenvironment of the cornea. In this study, the influence of surface topography and mechanical strain on keratocyte phenotype and ECM formation within a biomimetic 3D corneal model is studied. By modifying the surface topography of materials, it is found that patterned silk fibroin film with 600 grooves mm(-1) optimally supports cell alignment and ECM arrangement. Furthermore, treatment with 3% dome-shaped mechanical strain, which resembles the shape and mechanics of native cornea, significantly enhances the expression of keratocyte markers as compared to flat-shaped strain. Accordingly, a biomimetic 3D corneal model, in the form of a collagen-modified, silk fibroin-patterned construct subjected to 3% dome-shaped strain, is created. Compared to traditional 2D cultures, it supports a significantly higher expression of keratocyte and ECM markers, and in conclusion better maintains keratocyte phenotype, alignment, and fusiform cell shape. Therefore, the novel biomimetic 3D corneal model developed in this study serves as a useful in vitro 3D culture model to improve current 2D cultures for corneal studies.

Ort, förlag, år, upplaga, sidor
Wiley-VCH Verlagsgesellschaft, 2017
Nationell ämneskategori
Medicin och hälsovetenskap
Identifikatorer
urn:nbn:se:umu:diva-134745 (URN)10.1002/adhm.201601238 (DOI)000399716300010 ()2-s2.0-85008471524 (Scopus ID)
Tillgänglig från: 2017-05-11 Skapad: 2017-05-11 Senast uppdaterad: 2023-03-24Bibliografiskt granskad
Chen, J., Zhang, W., Liu, Z., Zhu, T., Shen, W., Ran, J., . . . Ouyang, H. (2016). Characterization and comparison of post-natal rat Achilles tendon-derived stem cells at different development stages. Scientific Reports, 6, Article ID 22946.
Öppna denna publikation i ny flik eller fönster >>Characterization and comparison of post-natal rat Achilles tendon-derived stem cells at different development stages
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2016 (Engelska)Ingår i: Scientific Reports, E-ISSN 2045-2322, Vol. 6, artikel-id 22946Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Tendon stem/progenitor cells (TSPCs) are a potential cell source for tendon tissue engineering. The striking morphological and structural changes of tendon tissue during development indicate the complexity of TSPCs at different stages. This study aims to characterize and compare post-natal rat Achilles tendon tissue and TSPCs at different stages of development. The tendon tissue showed distinct differences during development: the tissue structure became denser and more regular, the nuclei became spindle-shaped and the cell number decreased with time. TSPCs derived from 7 day Achilles tendon tissue showed the highest self-renewal ability, cell proliferation, and differentiation potential towards mesenchymal lineage, compared to TSPCs derived from 1 day and 56 day tissue. Microarray data showed up-regulation of several groups of genes in TSPCs derived from 7 day Achilles tendon tissue, which may account for the unique cell characteristics during this specific stage of development. Our results indicate that TSPCs derived from 7 day Achilles tendon tissue is a superior cell source as compared to TSPCs derived from 1 day and 56 day tissue, demonstrating the importance of choosing a suitable stem cell source for effective tendon tissue engineering and regeneration.

Nationell ämneskategori
Cell- och molekylärbiologi
Identifikatorer
urn:nbn:se:umu:diva-118964 (URN)10.1038/srep22946 (DOI)000371876900003 ()2-s2.0-84961189229 (Scopus ID)
Tillgänglig från: 2016-04-20 Skapad: 2016-04-07 Senast uppdaterad: 2023-03-23Bibliografiskt granskad
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