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Publikationer (10 of 14) Visa alla publikationer
Tjust, A. E., Hellman, U., Giannopoulos, A., Winsnes, A., Strigård, K. & Gunnarsson, U. (2024). Evaluation of extracellular matrix remodeling in full-thickness skin grafts in mice. Journal of Histochemistry and Cytochemistry, 72(2), 79-94
Öppna denna publikation i ny flik eller fönster >>Evaluation of extracellular matrix remodeling in full-thickness skin grafts in mice
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2024 (Engelska)Ingår i: Journal of Histochemistry and Cytochemistry, ISSN 0022-1554, E-ISSN 1551-5044, Vol. 72, nr 2, s. 79-94Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Abdominal hernia is a protruding weakness in the abdominal wall. It affects abdominal strength and life quality and can lead to complications due to intestinal entrapment. Autologous full-thickness skin graft (FTSG) has recently become an alternative material for reinforcement in the surgical repair of large abdominal hernias instead of synthetic mesh. FTSG eventually integrates with the abdominal wall, but the long-term fate of the graft itself is not fully understood. This has implications as to how these grafts should be optimally used and handled intraoperatively. This study investigates the remodeling of FTSG in either the onlay or the intraperitoneal position 8 weeks after FTSG transplantation in an experimental mouse model. There was a significant presence of fibroblasts, indicated by vimentin and S100A4 staining, but there were significant variations among animals as to how much of the graft had been remodeled into dense connective tissue. This correlated significantly with the proportion of vimentin-positive cells in the dense connective tissue. We also found that collagen hybridizing peptide staining intensity, a marker of active remodeling, was significantly associated with the proportion of S100A4-positive cells in the dense connective tissue of the FTSG.

Ort, förlag, år, upplaga, sidor
Sage Publications, 2024
Nyckelord
collagen, extracellular matrix, fibroblasts, hernia, hyaluronan, mouse model, S100A4, vimentin
Nationell ämneskategori
Kirurgi
Identifikatorer
urn:nbn:se:umu:diva-221051 (URN)10.1369/00221554231225995 (DOI)001147995400001 ()38264898 (PubMedID)2-s2.0-85184511984 (Scopus ID)
Forskningsfinansiär
Vetenskapsrådet, 2021-00972Region Västerbotten, RV-927121Region Västerbotten, RV-965797
Tillgänglig från: 2024-02-21 Skapad: 2024-02-21 Senast uppdaterad: 2024-02-21Bibliografiskt granskad
Behzadi, A., Tjust, A. E., Liu, J.-X., Andersen, P. M., Brännström, T. & Pedrosa Domellöf, F. (2023). Myofiber type shift in extraocular muscles in amyotrophic lateral sclerosis. Investigative Ophthalmology and Visual Science, 64(5), Article ID 15.
Öppna denna publikation i ny flik eller fönster >>Myofiber type shift in extraocular muscles in amyotrophic lateral sclerosis
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2023 (Engelska)Ingår i: Investigative Ophthalmology and Visual Science, ISSN 0146-0404, E-ISSN 1552-5783, Vol. 64, nr 5, artikel-id 15Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Purpose: To investigate changes in myofiber composition in the global layer (GL) and orbital layer (OL) of extraocular muscles (EOMs) from terminal amyotrophic lateral sclerosis (ALS) donors.

Methods: Medial recti muscles collected postmortem from spinal-onset ALS, bulbar-onset ALS, and healthy control donors were processed for immunofluorescence with antibodies against myosin heavy chain (MyHC) IIa, MyHCI, MyHCeom, laminin, neurofilaments, synaptophysin, acetylcholine receptor γ-subunit, and α-bungarotoxin.

Results: The proportion of myofibers containing MyHCIIa was significantly smaller and MyHCeom was significantly larger in the GL of spinal-onset ALS and bulbar-onset ALS donors compared to control donors. Changes in the GL were more prominent in the bulbar-onset ALS donors, with a significantly larger proportion of myofibers containing MyHCeom being present compared to spinal-onset ALS donors. There were no significant differences in the myofiber composition in the OL. In the spinal-onset ALS donors, the proportions of myofibers containing MyHCIIa in the GL and MyHCeom in the OL were significantly correlated with the disease duration. Neurofilament and synaptophysin were present at motor endplates of myofibers containing MyHCeom in ALS donors.

Conclusions: The EOMs of terminal ALS donors displayed changes in the fast-type myofiber composition in the GL, with a more pronounced alteration in bulbar-onset ALS donors. Our results align with the worse prognosis and subclinical changes in eye movement function previously observed in bulbar-onset ALS patients and suggest that the myofibers in the OL might be more resistant to the pathological process in ALS.

Ort, förlag, år, upplaga, sidor
Association for Research in Vision and Ophthalmology (ARVO), 2023
Nationell ämneskategori
Oftalmologi
Identifikatorer
urn:nbn:se:umu:diva-210285 (URN)10.1167/iovs.64.5.15 (DOI)37200039 (PubMedID)2-s2.0-85159762499 (Scopus ID)
Tillgänglig från: 2023-06-21 Skapad: 2023-06-21 Senast uppdaterad: 2024-03-26Bibliografiskt granskad
Behzadi, A., Pujol-Calderón, F., Tjust, A. E., Wuolikainen, A., Höglund, K., Forsberg, K., . . . Andersen, P. M. (2021). Neurofilaments can differentiate ALS subgroups and ALS from common diagnostic mimics. Scientific Reports, 11(1), Article ID 22128.
Öppna denna publikation i ny flik eller fönster >>Neurofilaments can differentiate ALS subgroups and ALS from common diagnostic mimics
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2021 (Engelska)Ingår i: Scientific Reports, E-ISSN 2045-2322, Vol. 11, nr 1, artikel-id 22128Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Delayed diagnosis and misdiagnosis are frequent in people with amyotrophic lateral sclerosis (ALS), the most common form of motor neuron disease (MND). Neurofilament light chain (NFL) and phosphorylated neurofilament heavy chain (pNFH) are elevated in ALS patients. We retrospectively quantified cerebrospinal fluid (CSF) NFL, CSF pNFH and plasma NFL in stored samples that were collected at the diagnostic work-up of ALS patients (n = 234), ALS mimics (n = 44) and controls (n = 9). We assessed the diagnostic performance, prognostication value and relationship to the site of onset and genotype. CSF NFL, CSF pNFH and plasma NFL levels were significantly increased in ALS patients compared to patients with neuropathies & myelopathies, patients with myopathies and controls. Furthermore, CSF pNFH and plasma NFL levels were significantly higher in ALS patients than in patients with other MNDs. Bulbar onset ALS patients had significantly higher plasma NFL levels than spinal onset ALS patients. ALS patients with C9orf72HRE mutations had significantly higher plasma NFL levels than patients with SOD1 mutations. Survival was negatively correlated with all three biomarkers. Receiver operating characteristics showed the highest area under the curve for CSF pNFH for differentiating ALS from ALS mimics and for plasma NFL for estimating ALS short and long survival. All three biomarkers have diagnostic value in differentiating ALS from clinically relevant ALS mimics. Plasma NFL levels can be used to differentiate between clinical and genetic ALS subgroups.

Ort, förlag, år, upplaga, sidor
Nature Publishing Group, 2021
Nationell ämneskategori
Neurologi
Identifikatorer
urn:nbn:se:umu:diva-189808 (URN)10.1038/s41598-021-01499-6 (DOI)000717747400105 ()2-s2.0-85118949353 (Scopus ID)
Tillgänglig från: 2021-11-22 Skapad: 2021-11-22 Senast uppdaterad: 2024-03-25Bibliografiskt granskad
Forsberg, K., Tjust, A. E., Zetterström, P., Marklund, S. L. & Andersen, P. M. (2020). ALS kan vara en prionsjukdom: Inklusioner av felvecklat SOD1-protein tycks finnas hos patienter med alla typer av ALS. Läkartidningen, 117, Article ID FYT4.
Öppna denna publikation i ny flik eller fönster >>ALS kan vara en prionsjukdom: Inklusioner av felvecklat SOD1-protein tycks finnas hos patienter med alla typer av ALS
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2020 (Svenska)Ingår i: Läkartidningen, ISSN 0023-7205, E-ISSN 1652-7518, Vol. 117, artikel-id FYT4Artikel i tidskrift (Refereegranskat) Published
Ort, förlag, år, upplaga, sidor
Läkartidningen Förlag AB, 2020
Nationell ämneskategori
Neurologi
Forskningsämne
neurologi
Identifikatorer
urn:nbn:se:umu:diva-179759 (URN)32154903 (PubMedID)2-s2.0-85081929668 (Scopus ID)
Anmärkning

Lakartidningen.se 2020-03-10

Tillgänglig från: 2021-02-09 Skapad: 2021-02-09 Senast uppdaterad: 2023-06-26Bibliografiskt granskad
Nilipour, Y., Nafissi, S., Tjust, A. E., Ravenscroft, G., Hossein Nejad Nedai, H., Taylor, R. L., . . . Tajsharghi, H. (2018). Ryanodine receptor type 3 (RYR3) as a novel gene associated with a myopathy with nemaline bodies. European Journal of Neurology, 25(6), 841-847
Öppna denna publikation i ny flik eller fönster >>Ryanodine receptor type 3 (RYR3) as a novel gene associated with a myopathy with nemaline bodies
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2018 (Engelska)Ingår i: European Journal of Neurology, ISSN 1351-5101, E-ISSN 1468-1331, Vol. 25, nr 6, s. 841-847Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

BACKGROUND AND PURPOSE: Nemaline myopathy (NEM) has been associated with mutations in 12 genes to date. However, for some patients diagnosed with NEM, definitive mutations are not identified in the known genes, suggesting that there are other genes involved. This study describes compound heterozygosity for rare variants in ryanodine receptor type 3 (RYR3) gene in one such patient.

METHODS AND RESULTS: Clinical examination of the patient at 22 years of age revealed a long narrow face, high arched palate and bilateral facial weakness. She had proximal weakness in all four limbs, mild scapular winging but no scoliosis. Muscle biopsy revealed wide variation in fibre size with type 1 fibre predominance and atrophy. Abundant nemaline bodies were located in perinuclear and subsarcolemmal areas, and within the cytoplasm. No likely pathogenic mutations in known NEM genes were identified. Copy number variation in known NEM genes was excluded by NEM-targeted comparative genomic hybridization array. Next-generation sequencing revealed compound heterozygous missense variants in the RYR3 gene. RYR3 transcripts are expressed in human fetal and adult skeletal muscle as well as in human brain and cauda equina samples. Immunofluorescence of human skeletal muscle revealed a 'single-row' appearance of RYR3, interspersed between the 'double rows' of ryanodine receptor type 1 (RYR1) at each A-I junction.

CONCLUSION: The results suggest that variants in RYR3 may cause a recessive muscle disease with pathological features including nemaline bodies. We characterize the expression pattern of RYR3 in human skeletal muscle and brain, and the subcellular localization of RYR1 and RYR3 in human skeletal muscle.

Nyckelord
RYR3, intracellular Ca2+ channels, nemaline myopathy, ryanodine receptors
Nationell ämneskategori
Endokrinologi och diabetes
Identifikatorer
urn:nbn:se:umu:diva-146323 (URN)10.1111/ene.13607 (DOI)000431989200012 ()29498452 (PubMedID)2-s2.0-85044433098 (Scopus ID)
Tillgänglig från: 2018-04-05 Skapad: 2018-04-05 Senast uppdaterad: 2023-06-26Bibliografiskt granskad
Tjust, A. (2017). Extraocular Muscles in Amyotrophic Lateral Sclerosis. (Doctoral dissertation). Umeå: Umeå universitet
Öppna denna publikation i ny flik eller fönster >>Extraocular Muscles in Amyotrophic Lateral Sclerosis
2017 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Amyotrophic lateral sclerosis (ALS) is an incurable neurodegenerative disease of motor neurons characterized by muscle paralysis and death within 3-5 years of onset. However, due to unknown mechanisms, the extraocular muscles (EOMs) remain remarkably unaffected. The EOMs are highly specialized muscles that differ from other muscles in many respects, including innervation and satellite cells (SCs). Understanding whether these factors play a role in the relative sparing of EOMs in ALS could provide useful clues on how to slow down the progression of ALS in other muscles.

The EOMs and limb muscles from terminal ALS patients and age-matched controls as well as the commonly used SOD1G93A ALS mouse model were studied with immunofluorescence. Antibodies against neurofilament and synaptophysin were used to identify nerves and neuromuscular junctions (NMJs); against Pax7, NCAM, MyoD, myogenin, Ki-67, dystrophin and laminin, to identify SCs and their progeny in EOMs and limb muscles. The proportion and fiber size of myofibers containing myosin heavy chain (MyHC) slow tonic and MyHC slow twitch were also determined in human EOMs.

The abundance of SCs differed extensively along the length of control human EOMs, being twice as abundant in the anterior portion. Pax7-positive cells were also detected in non-traditional SC positions. EOMs from terminal ALS patients showed similar numbers of resting and activated SCs as the controls. In limb muscles of ALS patients, the number of resting and activated SCs ranged from low (similar to normal aged, sedentary individuals) to high numbers, especially in muscles with long duration of disease and varied between the upper and lower limbs. The EOMs maintained a high degree of innervation compared to hindlimb muscles of symptomatic SOD1G93A mice. MyHC slow tonic fibers were less abundant in ALS patients than in controls. The change seemed more pronounced in bulbar onset patients, and in this group of subjects only, there was a strong association between decline in MyHC slow tonic fibers and age of death. Notably, the decline in MyHC slow tonic fibers was unrelated to disease duration.

Our data suggested that SCs play a minor role in the progression of ALS in general and in the sparing of the EOMs in particular. The generally preserved innervation in the EOMs of G93A mice may reflect distinct intrinsic properties relevant for sparing of the oculomotor system.  Even though the EOMs are relatively spared in ALS, MyHC slow tonic myofibers were selectively affected and this may reflect differences in innervation, as these fibers are multiply innervated.

Abstract [sv]

Amyotrofisk lateralskleros (ALS) är en obotlig neurodegenerativ sjukdom som främst påverkar kroppens viljestyrda motoriska nervceller. ALS leder till förlamning, muskelförtvining och slutligen döden genom andningssvikt, vanligen inom tre till fem år efter sjukdomsdebuten. Av okända anledningar så bibehålls ögonmusklernas funktion mycket bättre vid ALS i jämförelse med andra muskler och är hos merparten av patienter i stort sett opåverkade. Ögonmusklerna är mycket specialiserade muskler som skiljer sig från andra muskler i kroppen på flera sätt, bland annat genom deras unika nervförsörjning och genom de satellitceller – muskelspecifika stamceller, som finns i dem. En ökad förståelse för hur dessa faktorer inverkar på ögonmusklernas motståndskraft vid ALS skulle kunna ge värdefulla ledtrådar till hur man skulle kunna sakta ned sjukdomens fortskridande i andra muskler vid ALS.

Ögonmuskler och extremitetsmuskler från avlidna ALS-patienter och åldersmatchade friska kontroller, tillsammans med transgena möss med den sjukdomsalstrande mutationen SOD1G93A, studerades genom immunfluorescens och efterföljande mikroskopering. Antikroppar mot molekylerna Pax7, NCAM, MyoD, myogenin, Ki-67, laminin och dystrofin användes för att identifiera satellitceller och deras dotterceller i ögonmuskler och extremitetsmuskler. Antikroppar mot neurofilament och synaptofysin användes för att identifiera nerver och neuromuskulära synapser hos transgena SOD1-möss. Antikroppar mot toniska (tonic) och ryckande (twitch) muskelmyosinkedjor användes för att bestämma proportionen av och storleken på dessa typer av muskelfibrer i ögonmuskler från avlidna ALS-patienter och friska kontroller.

Mängden satellitceller varierade mellan de främre och de mer bakre delarna i friska, humana ögonmuskler och var dubbelt så många i den främre delen av muskeln jämfört med den mellersta och bakre delen av muskeln. Celler som uttryckte satellitcellsmarkören Pax7 hittades även i icke-traditionella satellitcellspositioner i ögonmusklerna. Mängden satellitceller i ögonmusklerna från ALS-patienter var samma som hos friska kontroller. I extremitetsmusklerna hos ALS-patienter varierade mängden satellitceller mellan låga nivåer (liknande de hos friska åldrade, inaktiva individer) till höga nivåer, särskilt i muskler där sjukdomen fortskridit under lång tid. Dessutom varierade mängden satellitceller mellan övre och nedre extremiteter.

Hos symptomatiska SOD1G93A-möss hade ögonmusklerna en mycket välbevarad innervation jämfört med bakbensmusklerna, där många neuromuskulära synapser saknade kontakt mellan nerven och motorändplattan.

Proportionen muskelfibrer med toniska muskelmyosinkedjor var lägre hos ALS-patienter jämfört med friska kontroller. Denna minskning var tydligare hos patienter där sjukdomssymtomen hade debuterat i tugg- och ansiktsmuskulaturen – så kallad bulbär ALS. Dessutom fanns det i den här gruppen, men ingen annan studerad grupp, en stark korrelation mellan nedgången i toniska fibrer och patientens ålder. Värt att notera är att minskningen av toniska muskelfibrer saknade korrelation med hur länge patienten hade varit sjuk i ALS.

Den generellt välbevarade innervationen i ögonmusklerna hos SOD1G93A-möss kan spegla distinkta inneboende egenskaper hos ögonmusklerna som är av vikt för bevarandet av ögonrörligheten vid ALS.

Gällande satellitceller så antyder våra data att satellitceller och deras regenerativa kapacitet spelar en försumbar roll vid ALS i allmänhet och vid ögonmusklernas bevarande i synnerhet.

Slutligen, även om ögonmuskler generellt är välbevarade vid ALS så är toniska muskelfibrer märkbart påverkade och detta kan spegla skillnader mellan olika nervcellsgruppers känslighet vid ALS.

Ort, förlag, år, upplaga, sidor
Umeå: Umeå universitet, 2017. s. 56
Serie
Umeå University medical dissertations, ISSN 0346-6612 ; 1854
Nyckelord
ALS, EOM, Satellite cells, innervation, neuromuscular disease, neurodegenerative disease
Nationell ämneskategori
Annan medicinsk grundvetenskap
Forskningsämne
anatomi
Identifikatorer
urn:nbn:se:umu:diva-129638 (URN)978-91-7601-618-3 (ISBN)
Disputation
2017-01-27, N320, Vån 3, Naturvetarhuset, Umeå universitet, Umeå, 09:00 (Engelska)
Opponent
Handledare
Forskningsfinansiär
Vetenskapsrådet, 2015-02438
Tillgänglig från: 2017-01-09 Skapad: 2017-01-06 Senast uppdaterad: 2018-06-09Bibliografiskt granskad
Tjust, A. E., Danielsson, A., Andersen, P. M., Brännstrom, T. & Pedrosa-Domellöf, F. (2017). Impact of Amyotrophic Lateral Sclerosis on Slow Tonic Myofiber Composition in Human Extraocular Muscles. Investigative Ophthalmology and Visual Science, 58(9), 3708-3715
Öppna denna publikation i ny flik eller fönster >>Impact of Amyotrophic Lateral Sclerosis on Slow Tonic Myofiber Composition in Human Extraocular Muscles
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2017 (Engelska)Ingår i: Investigative Ophthalmology and Visual Science, ISSN 0146-0404, E-ISSN 1552-5783, Vol. 58, nr 9, s. 3708-3715Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

PURPOSE. To analyze the proportion and cross-sectional area of myofibers containing myosin heavy chain slow-twitch (MyHCI) and myosin heavy chain slow tonic (MyHCsto) in extraocular muscles of autopsied amyotrophic lateral sclerosis (ALS) patients with either spinal or bulbar site of disease onset. METHODS. Whole-muscle cross sections from the middle portion of the medial rectus were labeled with antibodies against MyHCI or MyHCsto and laminin. Myofibers labeled with the MyHC antibodies (MyHCI+sto) and the total number of myofibers were quantified in the orbital and global layer of 6 control individuals and 18 ALS patients. The cross-sectional area of myofibers labeled for either MyHC was quantified in 130 to 472 fibers/individual in the orbital and in 180 to 573 fibers/individual in the global layer of each specimen. RESULTS. The proportion of MyHCI+sto myofibers was significantly smaller in the orbital and global layer of ALS compared to control individuals. MyHCI+sto myofibers were significantly smaller in the global layer than in the orbital layer of ALS, whereas they were of similar size in control subjects. The decreased proportion of MyHCI+sto fibers correlated significantly with the age of death, but not disease duration, in patients who had the bulbar-onset variant of ALS but not in patients with spinal variant. CONCLUSIONS. ALS, regardless of site of onset, involves a loss of myofibers containing MyHCI+sto. Only in bulbar-onset cases did aging seem to play a role in the pathophysiological processes underlying the loss of MyHCI+sto fibers.

Ort, förlag, år, upplaga, sidor
ASSOC RESEARCH VISION OPHTHALMOLOGY INC, 2017
Nyckelord
amyotrophic lateral sclerosis, extraocular muscles, MyH14, slow tonic, muscle fibers
Nationell ämneskategori
Oftalmologi
Identifikatorer
urn:nbn:se:umu:diva-140482 (URN)10.1167/iovs.17-22098 (DOI)000410931200051 ()28738414 (PubMedID)2-s2.0-85026313703 (Scopus ID)
Tillgänglig från: 2017-10-20 Skapad: 2017-10-20 Senast uppdaterad: 2023-06-26Bibliografiskt granskad
Tjust, A. E., Danielsson, A., Andersen, P. M. & Pedrosa-Domellöf, F. (2016). Loss of myofibers containing MyHC Slow tonic in extraocular muscles of terminal ALS patients with bulbar onset. Paper presented at Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO), MAY 01-05, 2016, Seattle, WA. Investigative Ophthalmology and Visual Science, 57(12)
Öppna denna publikation i ny flik eller fönster >>Loss of myofibers containing MyHC Slow tonic in extraocular muscles of terminal ALS patients with bulbar onset
2016 (Engelska)Ingår i: Investigative Ophthalmology and Visual Science, ISSN 0146-0404, E-ISSN 1552-5783, Vol. 57, nr 12Artikel i tidskrift, Meeting abstract (Refereegranskat) Published
Ort, förlag, år, upplaga, sidor
ASSOC RESEARCH VISION OPHTHALMOLOGY INC, 2016
Nationell ämneskategori
Oftalmologi
Identifikatorer
urn:nbn:se:umu:diva-132855 (URN)000394210604177 ()
Konferens
Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO), MAY 01-05, 2016, Seattle, WA
Tillgänglig från: 2017-03-23 Skapad: 2017-03-23 Senast uppdaterad: 2023-06-26Bibliografiskt granskad
Lindström, M., Tjust, A. E. & Domellöf, F. P. (2015). Pax7-Positive Cells/Satellite Cells in Human Extraocular Muscles. Investigative Ophthalmology and Visual Science, 56(10), 6132-6143
Öppna denna publikation i ny flik eller fönster >>Pax7-Positive Cells/Satellite Cells in Human Extraocular Muscles
2015 (Engelska)Ingår i: Investigative Ophthalmology and Visual Science, ISSN 0146-0404, E-ISSN 1552-5783, Vol. 56, nr 10, s. 6132-6143Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

PURPOSE. We quantified and investigated the distribution of Pax7-positive cells/satellite cells (SCs) in the human extraocular muscles (EOMs). METHODS. An immunofluorescence multiple-marker method simultaneously combining two SC markers (Pax7, NCAM), detection of the basement membrane (laminin) and cell nuclei (4',6-diamidino-2-phenylindole [DAPI]), was used on the anterior, middle, and posterior portions of EOMs from five healthy donors. Pax7-positive cell and SC content, myonuclear content, myofiber cross-sectional area, and myonuclear domain were analyzed in single cross-sections. Between 3915 and 13,536 myofibers per muscle cross-section and myofibers from the entire EOM cross-section were analyzed for quantification of Pax7-positive cells per myofiber (Pax7/F).

RESULTS. The number of Pax7/F in the human EOMs varies along the length of the muscle with twice as high Pax7/F in the anterior part of the EOMs, but within the range of what has been previously reported for normal adult limb muscles. Furthermore, there are Pax7-positive cells in positions other than the classical SC position and the myonuclear domain size of adult EOMs is noticeably smaller than that previously reported for other adult skeletal muscles.

CONCLUSIONS. Previous data on differences in Pax7-positive cell/SC abundance between EOMs and limb muscles must be reconsidered and the characteristics of different Pax7-positive cell populations further investigated. Higher numbers of Pax7-positive cells in the anterior portion of the EOMs may have a bearing for strabismus surgery involving sectioning of the muscle fibers.

Nyckelord
satellite cell, myoprogenitor cell, Pax7
Nationell ämneskategori
Mikrobiologi inom det medicinska området
Identifikatorer
urn:nbn:se:umu:diva-116774 (URN)10.1167/iovs.15-16544 (DOI)000368426300057 ()26393672 (PubMedID)2-s2.0-84942155464 (Scopus ID)
Forskningsfinansiär
Vetenskapsrådet, K2012-63x-20399-06-3
Tillgänglig från: 2016-02-15 Skapad: 2016-02-11 Senast uppdaterad: 2023-06-26Bibliografiskt granskad
Tjust, A. E., Brännstrom, T. & Pedrosa Domellöf, F. (2012). Unaffected motor endplate occupancy in eye muscles of ALS G93A mouse model. Frontiers in bioscience (Scholar edition), 4, 1547-1555
Öppna denna publikation i ny flik eller fönster >>Unaffected motor endplate occupancy in eye muscles of ALS G93A mouse model
2012 (Engelska)Ingår i: Frontiers in bioscience (Scholar edition), ISSN 1945-0524, Vol. 4, s. 1547-1555Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Amyotrophic lateral sclerosis (ALS) is a progressive, lethal neurodegenerative disorder characterised by selective loss of motor neurons with accompanying muscle paralysis and respiratory failure. Despite progressive paralysis in trunk and extremity muscles, disturbed eye motility is not a hallmark of ALS. Extraocular muscles (EOMs) of terminal ALS patients show far less morphological signs of disease than their limb muscles. One of the earliest signs of the disease in the transgenic G93A SOD1 mouse model of ALS is loss of motor neuron contact at the neuromuscular junctions (NMJ) in limb muscles. We used immunohistochemistry to identify NMJs and evaluate innervation in EOMs and limb muscles of G93A mice. In G93A limb muscles, loss of axonal contact was seen in 6-82 percent of the NMJs. On the contrary, the degree of endplate occupancy in the EOMs did not differ between transgenic mice and wild-type controls. We propose that EOM-specific properties make these muscles more resistant to the underlying pathophysiological process of ALS and that the EOMs are a useful model to advance our understanding of ALS.

Ort, förlag, år, upplaga, sidor
Frontiers in Bioscience, 2012
Nyckelord
Amyotropic lateral sclerosis, Neuromuscular junction, Extraocular muscle, SOD1, G93A, Denervation, Synaptophysin
Nationell ämneskategori
Medicinska och farmaceutiska grundvetenskaper
Identifikatorer
urn:nbn:se:umu:diva-73222 (URN)22652891 (PubMedID)2-s2.0-84869471236 (Scopus ID)
Tillgänglig från: 2013-06-19 Skapad: 2013-06-19 Senast uppdaterad: 2023-06-26Bibliografiskt granskad
Organisationer
Identifikatorer
ORCID-id: ORCID iD iconorcid.org/0000-0003-2369-4319

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