Umeå University's logo

umu.sePublications
Change search
Link to record
Permanent link

Direct link
Publications (10 of 18) Show all publications
Donev, E. N., Derba-Maceluch, M., Yassin, Z., Gandla, M. L., Pramod, S., Heinonen, E., . . . Mellerowicz, E. J. (2023). Field testing of transgenic aspen from large greenhouse screening identifies unexpected winners. Plant Biotechnology Journal, 21(5), 1005-1021
Open this publication in new window or tab >>Field testing of transgenic aspen from large greenhouse screening identifies unexpected winners
Show others...
2023 (English)In: Plant Biotechnology Journal, ISSN 1467-7644, E-ISSN 1467-7652, Vol. 21, no 5, p. 1005-1021Article in journal (Refereed) Published
Abstract [en]

Trees constitute promising renewable feedstocks for biorefinery using biochemical conversion, but their recalcitrance restricts their attractiveness for the industry. To obtain trees with reduced recalcitrance, large-scale genetic engineering experiments were performed in hybrid aspen blindly targeting genes expressed during wood formation and 32 lines representing seven constructs were selected for characterization in the field. Here we report phenotypes of five-year old trees considering 49 traits related to growth and wood properties. The best performing construct considering growth and glucose yield in saccharification with acid pretreatment had suppressed expression of the gene encoding an uncharacterized 2-oxoglutarate-dependent dioxygenase (2OGD). It showed minor changes in wood chemistry but increased nanoporosity and glucose conversion. Suppressed levels of SUCROSE SYNTHASE, (SuSy), CINNAMATE 4-HYDROXYLASE (C4H) and increased levels of GTPase activating protein for ADP-ribosylation factor ZAC led to significant growth reductions and anatomical abnormalities. However, C4H and SuSy constructs greatly improved glucose yields in saccharification without and with pretreatment, respectively. Traits associated with high glucose yields were different for saccharification with and without pretreatment. While carbohydrates, phenolics and tension wood contents positively impacted the yields without pretreatment and growth, lignin content and S/G ratio were negative factors, the yields with pretreatment positively correlated with S lignin and negatively with carbohydrate contents. The genotypes with high glucose yields had increased nanoporosity and mGlcA/Xyl ratio, and some had shorter polymers extractable with subcritical water compared to wild-type. The pilot-scale industrial-like pretreatment of best-performing 2OGD construct confirmed its superior sugar yields, supporting our strategy.

Place, publisher, year, edition, pages
John Wiley & Sons, 2023
Keywords
enzymatic saccharification, field trial, secondary cell wall, SilviScan, subcritical water extraction, transgenic Populus
National Category
Plant Biotechnology
Identifiers
urn:nbn:se:umu:diva-204767 (URN)10.1111/pbi.14012 (DOI)000925931700001 ()36668687 (PubMedID)2-s2.0-85147264638 (Scopus ID)
Funder
Knut and Alice Wallenberg FoundationVinnovaSwedish Foundation for Strategic Research, RBP14-0011Bio4EnergySwedish University of Agricultural Sciences
Available from: 2023-02-20 Created: 2023-02-20 Last updated: 2023-11-06Bibliographically approved
Urbancsok, J., Donev, E. N., Sivan, P., van Zalen, E., Barbut, F. R., Derba-Maceluch, M., . . . Mellerowicz, E. J. (2023). Flexure wood formation via growth reprogramming in hybrid aspen involves jasmonates and polyamines and transcriptional changes resembling tension wood development. New Phytologist, 240(6), 2312-2334
Open this publication in new window or tab >>Flexure wood formation via growth reprogramming in hybrid aspen involves jasmonates and polyamines and transcriptional changes resembling tension wood development
Show others...
2023 (English)In: New Phytologist, ISSN 0028-646X, E-ISSN 1469-8137, Vol. 240, no 6, p. 2312-2334Article in journal (Refereed) Published
Abstract [en]

Stem bending in trees induces flexure wood but its properties and development are poorly understood. Here, we investigated the effects of low-intensity multidirectional stem flexing on growth and wood properties of hybrid aspen, and on its transcriptomic and hormonal responses.

Glasshouse-grown trees were either kept stationary or subjected to several daily shakes for 5 wk, after which the transcriptomes and hormones were analyzed in the cambial region and developing wood tissues, and the wood properties were analyzed by physical, chemical and microscopy techniques.

Shaking increased primary and secondary growth and altered wood differentiation by stimulating gelatinous-fiber formation, reducing secondary wall thickness, changing matrix polysaccharides and increasing cellulose, G- and H-lignin contents, cell wall porosity and saccharification yields. Wood-forming tissues exhibited elevated jasmonate, polyamine, ethylene and brassinosteroids and reduced abscisic acid and gibberellin signaling. Transcriptional responses resembled those during tension wood formation but not opposite wood formation and revealed several thigmomorphogenesis-related genes as well as novel gene networks including FLA and XTH genes encoding plasma membrane-bound proteins.

Low-intensity stem flexing stimulates growth and induces wood having improved biorefinery properties through molecular and hormonal pathways similar to thigmomorphogenesis in herbaceous plants and largely overlapping with the tension wood program of hardwoods.

Place, publisher, year, edition, pages
John Wiley & Sons, 2023
Keywords
flexure wood, jasmonic acid signaling, mechanostimulation, polyamines, Populus tremula × tremuloides, saccharification, thigmomorphogenesis, wood development
National Category
Botany Wood Science
Identifiers
urn:nbn:se:umu:diva-215853 (URN)10.1111/nph.19307 (DOI)001085559700001 ()37857351 (PubMedID)2-s2.0-85174386995 (Scopus ID)
Funder
Knut and Alice Wallenberg FoundationSwedish Research CouncilBio4Energy
Available from: 2023-11-06 Created: 2023-11-06 Last updated: 2024-07-02Bibliographically approved
Winestrand, S., Järnström, L. & Jönsson, L. J. (2021). Fractionated lignosulfonates for laccase-catalyzed oxygen-scavenging films and coatings. Molecules, 26(20), Article ID 6322.
Open this publication in new window or tab >>Fractionated lignosulfonates for laccase-catalyzed oxygen-scavenging films and coatings
2021 (English)In: Molecules, ISSN 1431-5157, E-ISSN 1420-3049, Vol. 26, no 20, article id 6322Article in journal (Refereed) Published
Abstract [en]

Lignin derivatives have potential as antioxidants in advanced packaging materials through their ability to scavenge oxygen in reactions catalyzed by phenol-oxidizing enzymes, such as laccase. The effects of size fractionation of lignosulfonates on laccase-catalyzed reactions were investigated in experiments with aqueous solutions, films, and coated paperboard. Four industrial lignosulfonate preparations were compared: Feed (unfractionated), Prod (5–60 kDa enriched), Conc (≥60 kDa enriched), and Perm (≤60 kDa enriched). Extraction of lignosulfonates from films showed that the enzymic reaction increased the average molecular weight from <10,000 to up to 66,000. The enzymatic reaction resulted in an increase in the water contact angle of the films from the range 25–49◦ to 56–81◦. The four preparations showed relatively small differences with regard to their ability to scavenge oxygen in aqueous solution and in experiments with coated paperboards in sealed chambers. Coatings with lignosulfonates where the contents of low-molecular weight material had been reduced (i.e., Prod and Conc) showed improved water resistance after the enzymic reaction. Thus, in both aqueous and solid media, fractionation of lignosulfonates had little effect on oxygen scavenging, but fractionation was beneficial for other reasons, such as improved cross-linking resulting in higher molecular weight and superior water resistance.

Place, publisher, year, edition, pages
MDPI, 2021
Keywords
Active packaging, Coating, Laccase, Lignin, Lignosulfonates, Oxygen scavenging, Paperboard
National Category
Polymer Chemistry
Identifiers
urn:nbn:se:umu:diva-188953 (URN)10.3390/molecules26206322 (DOI)000794102200001 ()2-s2.0-85117489972 (Scopus ID)
Funder
The Kempe Foundations
Available from: 2021-10-29 Created: 2021-10-29 Last updated: 2023-11-06Bibliographically approved
Pramod, S., Gandla, M. L., Derba-Maceluch, M., Jönsson, L. J., Mellerowicz, E. J. & Winestrand, S. (2021). Saccharification Potential of Transgenic Greenhouse- and Field-Grown Aspen Engineered for Reduced Xylan Acetylation. Frontiers in Plant Science, 12, Article ID 704960.
Open this publication in new window or tab >>Saccharification Potential of Transgenic Greenhouse- and Field-Grown Aspen Engineered for Reduced Xylan Acetylation
Show others...
2021 (English)In: Frontiers in Plant Science, E-ISSN 1664-462X, Vol. 12, article id 704960Article in journal (Refereed) Published
Abstract [en]

High acetylation of xylan in hardwoods decreases their value as biorefinery feedstocks. To counter this problem, we have constitutively suppressed RWA genes encoding acetyl-CoA transporters using the 35S promoter, or constitutively and wood-specifically (using the WP promoter) expressed fungal acetyl xylan esterases of families CE1 (AnAXE1) and CE5 (HjAXE), to reduce acetylation in hybrid aspen. All these transformations improved the saccharification of wood from greenhouse-grown trees. Here, we describe the chemical properties and saccharification potential of the resulting lines grown in a five-year field trial, and one type of them (WP:AnAXE1) in greenhouse conditions. Chemically, the lignocellulose of the field- and greenhouse-field-grown plants slightly differed, but the reductions in acetylation and saccharification improvement of engineered trees were largely maintained in the field. The main novel phenotypic observation in the field was higher lignification in lines with the WP promoter than those with the 35S promoter. Following growth in the field, saccharification glucose yields were higher from most transformed lines than from wild-type (WT) plants with no pretreatment, but there was no improvement in saccharification with acid pretreatment. Thus, acid pretreatment removes most recalcitrance caused by acetylation. We found a complex relationship between acetylation and glucose yields in saccharification without pretreatment, suggesting that other variables, for example, the acetylation pattern, affect recalcitrance. Bigger gains in glucose yields were observed in lines with the 35S promoter than in those with the WP promoter, possibly due to their lower lignin content. However, better lignocellulose saccharification of these lines was offset by a growth penalty and their glucose yield per tree was lower. In a comparison of the best lines with each construct, WP:AnAXE1 provided the highest glucose yield per tree from saccharification, with and without pretreatment, WP:HjAXE yields were similar to those of WT plants, and yields of lines with other constructs were lower. These results show that lignocellulose properties of field-grown trees can be improved by reducing cell wall acetylation using various approaches, but some affect productivity in the field. Thus, better understanding of molecular and physiological consequences of deacetylation is needed to obtain quantitatively better results.

Place, publisher, year, edition, pages
Frontiers Media S.A., 2021
Keywords
cell wall acetylation, field trial, genetic modification trees, lignocellulose, Populus tremula × tremuloides, saccharification, T89, wood
National Category
Plant Biotechnology
Identifiers
urn:nbn:se:umu:diva-187996 (URN)10.3389/fpls.2021.704960 (DOI)000697585200001 ()34557213 (PubMedID)2-s2.0-85115423455 (Scopus ID)
Funder
Swedish Foundation for Strategic Research , RBP14-0011
Available from: 2021-09-30 Created: 2021-09-30 Last updated: 2024-07-02Bibliographically approved
Wang, Z., Winestrand, S., Gillgren, T. & Jönsson, L. J. (2018). Chemical and structural factors influencing enzymatic saccharification of wood from aspen, birch and spruce. Biomass and Bioenergy, 109, 125-134
Open this publication in new window or tab >>Chemical and structural factors influencing enzymatic saccharification of wood from aspen, birch and spruce
2018 (English)In: Biomass and Bioenergy, ISSN 0961-9534, E-ISSN 1873-2909, Vol. 109, p. 125-134Article in journal (Refereed) Published
Abstract [en]

The susceptibility of untreated and sulfuric-acid-pretreated aspen, birch, and spruce to analytical enzymatic saccharification was studied in relation to their chemical composition and physical-structural features. The analytical data collected covered the mass fractions of lignin, carbohydrates, and extractives, the release of acetic acid, formic acid, and uronic acids by acid and alkaline hydrolysis, crystallinity and crystallite size, syringyl: guaiacyl (S:G) ratio of lignin, cellulose accessibility, FTIR spectra, images from SEM and fluorescence microscopy, and susceptibility to enzymatic saccharification using enzyme mixtures with and without supplementary xylanase.In the absence of pretreatment the mass fraction yield of Glc on the original dry wood in the analytical enzymatic saccharification increased in the order birch (16 g kg−1) < spruce (35 g kg−1) < aspen (150 g kg−1). After acid pretreatment, the order changed to spruce (170 g kg−1) < aspen (290 g kg−1), birch (290 g kg−1). The relatively high recalcitrance of untreated birch was not possible to relate to mass fraction of lignin, S:G ratio, cellulose crystallinity, or mass fraction of acetyl, but rather to structural features, such as a more compact surface structure with high density and low cellulose accessibility. The relatively high sugar yields from both untreated and pretreated aspen suggest that aspen wood is well suited as feedstock for production of liquid biofuels and green chemicals in forest-based biorefineries.

Place, publisher, year, edition, pages
Elsevier, 2018
Keywords
Hardwood, Softwood, Chemical composition, Dilute-acid pretreatment, Enzymatic saccharification, Physical structure
National Category
Paper, Pulp and Fiber Technology
Identifiers
urn:nbn:se:umu:diva-143889 (URN)10.1016/j.biombioe.2017.12.020 (DOI)000424939200015 ()2-s2.0-85039997506 (Scopus ID)
Projects
Bio4Energy
Funder
Bio4Energy
Available from: 2018-01-12 Created: 2018-01-12 Last updated: 2024-07-02Bibliographically approved
Ratke, C., Terebieniec, B. K., Winestrand, S., Derba-Maceluch, M., Grahn, T., Schiffthaler, B., . . . Mellerowicz, E. J. (2018). Downregulating aspen xylan biosynthetic GT43 genes in developing wood stimulates growth via reprograming of the transcriptome. New Phytologist, 219(1), 230-245
Open this publication in new window or tab >>Downregulating aspen xylan biosynthetic GT43 genes in developing wood stimulates growth via reprograming of the transcriptome
Show others...
2018 (English)In: New Phytologist, ISSN 0028-646X, Vol. 219, no 1, p. 230-245Article in journal (Refereed) Published
Abstract [en]

Xylan is one of the main compounds determining wood properties in hardwood species. The xylan backbone is thought to be synthesized by a synthase complex comprising two members of the GT43 family. We downregulated all GT43 genes in hybrid aspen (Populus tremulaxtremuloides) to understand their involvement in xylan biosynthesis.

All three clades of the GT43 family were targeted for downregulation using RNA interference individually or in different combinations, either constitutively or specifically in developing wood.

Simultaneous downregulation in developing wood of the B (IRX9) and C (IRX14) clades resulted in reduced xylan Xyl content relative to reducing end sequence, supporting their role in xylan backbone biosynthesis. This was accompanied by a higher lignocellulose saccharification efficiency. Unexpectedly, GT43 suppression in developing wood led to an overall growth stimulation, xylem cell wall thinning and a shift in cellulose orientation. Transcriptome profiling of these transgenic lines indicated that cell cycling was stimulated and secondary wall biosynthesis was repressed. We suggest that the reduced xylan elongation is sensed by the cell wall integrity surveying mechanism in developing wood.

Our results show that wood-specific suppression of xylan-biosynthetic GT43 genes activates signaling responses, leading to increased growth and improved lignocellulose saccharification.

Keywords
cellulose microfibril angle, GT43, Populus, saccharification, secondary wall, wood development, xylan biosynthesis
National Category
Plant Biotechnology
Identifiers
urn:nbn:se:umu:diva-150384 (URN)10.1111/nph.15160 (DOI)000434153200026 ()29708593 (PubMedID)2-s2.0-85046148362 (Scopus ID)
Projects
Bio4Energy
Funder
Bio4Energy
Available from: 2018-08-06 Created: 2018-08-06 Last updated: 2024-07-02Bibliographically approved
Wang, Z., Gräsvik, J., Jönsson, L. J. & Winestrand, S. (2017). Comparison of [HSO4](-), [Cl](-) and [MeCO2](-) as anions in pretreatment of aspen and spruce with imidazolium-based ionic liquids. BMC Biotechnology, 17, Article ID 82.
Open this publication in new window or tab >>Comparison of [HSO4](-), [Cl](-) and [MeCO2](-) as anions in pretreatment of aspen and spruce with imidazolium-based ionic liquids
2017 (English)In: BMC Biotechnology, E-ISSN 1472-6750, Vol. 17, article id 82Article in journal (Refereed) Published
Abstract [en]

Background: Ionic liquids (ILs) draw attention as green solvents for pretreatment of lignocellulose before enzymatic saccharification. Imidazolium-based ILs with different anionic constituents ([HSO4], [Cl], [MeCO2]) were compared with regard to pretreatment of wood from aspen and spruce. The objective was to elucidate how the choice of anionic constituent affected the suitability of using the IL for pretreatment of hardwood, such as aspen, and softwood, such as spruce. The investigation covered a thorough analysis of the mass balance of the IL pretreatments, the effects of pretreatment on the cell wall structure as assessed by fluorescence microscopy, and the effects of pretreatment on the susceptibility to enzymatic saccharification. Torrefied aspen and spruce were included in the comparison for assessing how shifting contents of hemicelluloses and Klason lignin affected the susceptibility of the wood to IL pretreatment and enzymatic saccharification.

Results: The glucose yield after IL pretreatment increased in the order [Cl] < [HSO4] < [MeCO2] for aspen, but in the order [HSO4] < [Cl] < [MeCO2] for spruce. For both aspen and spruce, removal of hemicelluloses and lignin increased in the order [Cl] < [MeCO2] < [HSO4]. Fluorescence microscopy indicated increasingly disordered cell wall structure following the order [HSO4] < [Cl] < [MeCO2]. Torrefaction of aspen converted xylan to pseudo-lignin and changed the glucose yield order to [HSO4] < [Cl] < [MeCO2].

Conclusions: The acidity of [HSO4] caused extensive hydrolysis of xylan, which facilitated pretreatment of xylan-rich hardwood. Apart from that, the degree of removal of hemicelluloses and lignin did not correspond well with the improvement of the enzymatic saccharification. Taken together, the saccharification results were found to mainly reflect (i) the different capacities of the ILs to disorder the cell wall structure, (ii) the recalcitrance caused by high xylan content, and (iii) the capacity of the [HSO4]-based IL to hydrolyze xylan.

Place, publisher, year, edition, pages
BioMed Central (BMC), 2017
Keywords
Hardwood, Softwood, Pretreatment, Ionic liquid, Enzymatic saccharification, Torrefaction, Xylan, ason lignin, Pseudo-lignin
National Category
Biochemistry Molecular Biology
Identifiers
urn:nbn:se:umu:diva-142457 (URN)10.1186/s12896-017-0403-0 (DOI)000415149600001 ()29141617 (PubMedID)2-s2.0-85034584954 (Scopus ID)
Projects
Bio4Energy
Funder
Bio4Energy
Available from: 2017-12-04 Created: 2017-12-04 Last updated: 2025-02-20Bibliographically approved
Normark, M., Pommer, L., Gräsvik, J., Hedenström, M., Gorzsas, A., Winestrand, S. & Jönsson, L. J. (2016). Biochemical Conversion of Torrefied Norway Spruce After Pretreatment with Acid or Ionic Liquid. Bioenergy Research, 9(1), 355-368
Open this publication in new window or tab >>Biochemical Conversion of Torrefied Norway Spruce After Pretreatment with Acid or Ionic Liquid
Show others...
2016 (English)In: Bioenergy Research, ISSN 1939-1234, E-ISSN 1939-1242, Vol. 9, no 1, p. 355-368Article in journal (Refereed) Published
Abstract [en]

The chemical effects of torrefaction and the possibility to combine torrefaction with biochemical conversion were explored in experiments with five preparations of wood of Norway spruce that had been torrefied using different degrees of severity. Compositional analysis and analyses using solid-state CP/MAS C-13 NMR, Fourier-transform infrared (FTIR) spectroscopy, and Py-GC/MS showed small gradual changes, such as decreased hemicellulosic content and increased Klason lignin value, for torrefaction conditions in the range from 260 A degrees C and 8 min up to 310 A degrees C and 8 min. The most severe torrefaction conditions (310 A degrees C, 25 min) resulted in substantial loss of glucan and further increase of the Klason lignin value, which was attributed to conversion of carbohydrate to pseudo-lignin. Even mild torrefaction conditions led to decreased susceptibility to enzymatic hydrolysis of cellulose, a state which was not changed by pretreatment with sulfuric acid. Pretreatment with the ionic liquid (IL) 1-butyl-3-methylimidazolium acetate overcame the additional recalcitrance caused by torrefaction, and the glucose yields after 72 h of enzymatic hydrolysis of wood torrefied at 260 A degrees C for 8 min and at 285 A degrees C for 16.5 min were as high as that of IL-pretreated non-torrefied spruce wood. Compared to IL-pretreated non-torrefied reference wood, the glucose production rates after 2 h of enzymatic hydrolysis of IL-pretreated wood torrefied at 260 A degrees C for 8 min and at 285 A degrees C for 16.5 min were 63 and 40 % higher, respectively. The findings offer increased understanding of the effects of torrefaction and indicate that mild torrefaction is compatible with biochemical conversion after pretreatment with alternative solvents that disrupt pseudo-lignin-containing lignocellulose.

Keywords
Torrefaction, Wood, Acid pretreatment, Ionic liquid, Enzymatic hydrolysis
National Category
Other Chemistry Topics
Identifiers
urn:nbn:se:umu:diva-118386 (URN)10.1007/s12155-015-9698-7 (DOI)000370816300032 ()2-s2.0-84959173363 (Scopus ID)
Projects
Bio4Energy
Funder
Bio4Energy
Available from: 2016-04-22 Created: 2016-03-18 Last updated: 2024-07-02Bibliographically approved
Normark, M., Winestrand, S., Lestander, T. A. & Jönsson, L. J. (2014). Analysis, pretreatment and enzymatic saccharification of different fractions of Scots pine. BMC Biotechnology, 14, Article ID 20.
Open this publication in new window or tab >>Analysis, pretreatment and enzymatic saccharification of different fractions of Scots pine
2014 (English)In: BMC Biotechnology, E-ISSN 1472-6750, Vol. 14, article id 20Article in journal (Refereed) Published
Abstract [en]

Background: Forestry residues consisting of softwood are a major lignocellulosic resource for production of liquid biofuels. Scots pine, a commercially important forest tree, was fractionated into seven fractions of chips: juvenile heartwood, mature heartwood, juvenile sapwood, mature sapwood, bark, top parts, and knotwood. The different fractions were characterized analytically with regard to chemical composition and susceptibility to dilute-acid pretreatment and enzymatic saccharification. Results: All fractions were characterized by a high glucan content (38-43%) and a high content of other carbohydrates (11-14% mannan, 2-4% galactan) that generate easily convertible hexose sugars, and by a low content of inorganic material (0.2-0.9% ash). The lignin content was relatively uniform (27-32%) and the syringyl-guaiacyl ratio of the different fractions were within the range 0.021-0.025. The knotwood had a high content of extractives (9%) compared to the other fractions. The effects of pretreatment and enzymatic saccharification were relatively similar, but without pretreatment the bark fraction was considerably more susceptible to enzymatic saccharification. Conclusions: Since sawn timber is a main product from softwood species such as Scots pine, it is an important issue whether different parts of the tree are equally suitable for bioconversion processes. The investigation shows that bioconversion of Scots pine is facilitated by that most of the different fractions exhibit relatively similar properties with regard to chemical composition and susceptibility to techniques used for bioconversion of woody biomass.

Place, publisher, year, edition, pages
BioMed Central (BMC), 2014
Keywords
Scots pine, Chemical composition, Dilute-acid pretreatment, Enzymatic saccharification
National Category
Microbiology Agricultural Biotechnology
Identifiers
urn:nbn:se:umu:diva-89235 (URN)10.1186/1472-6750-14-20 (DOI)000334881900001 ()2-s2.0-84898867964 (Scopus ID)
Available from: 2014-05-26 Created: 2014-05-26 Last updated: 2024-07-02Bibliographically approved
Johansson, K., Gillgren, T., Winestrand, S., Jarnstrom, L. & Jönsson, L. (2014). Comparison of lignin derivatives as substrates for laccase-catalyzed scavenging of oxygen in coatings and films. Journal of Biological Engineering, 8(1)
Open this publication in new window or tab >>Comparison of lignin derivatives as substrates for laccase-catalyzed scavenging of oxygen in coatings and films
Show others...
2014 (English)In: Journal of Biological Engineering, E-ISSN 1754-1611, Vol. 8, no 1Article in journal (Refereed) Published
Abstract [en]

Background:

Lignin derivatives are phenylpropanoid biopolymers derived from pulping and biorefinery processes. The possibility to utilize lignin derivatives from different types of processes in advanced enzyme-catalyzed oxygen-scavenging systems intended for active packaging was explored. Laccase-catalyzed oxidation of alkali lignin (LA), hydrolytic lignin (LH), organosolv lignin (LO), and lignosulfonates (LS) was compared using oxygen-scavenging coatings and films in liquid and gas phase systems.

Results:

When coatings containing lignin derivatives and laccase were immersed in a buffered aqueous solution, the oxygen-scavenging capability increased in the order LO < LH < LA < LS. Experiments with coatings containing laccase and LO, LH or LA incubated in oxygen-containing gas in air-tight chambers and at a relative humidity (RH) of 100% showed that paperboard coated with LO and laccase reduced the oxygen content from 1.0% to 0.4% during a four-day period, which was far better than the results obtained with LA or LH. LO-containing coatings incubated at 92% RH also displayed activity, with a decrease in oxygen from 1.0% to 0.7% during a four-day period. The oxygen scavenging was not related to the content of free phenolic hydroxyl groups, which increased in the order LO < LS < LH < LA. LO and LS were selected for further studies and films containing starch, clay, glycerol, laccase and LO or LS were characterized using gel permeation chromatograpy, dynamic mechanical analysis, and wet stability.

Conclusions:

The investigation shows that different lignin derivatives exhibit widely different properties as a part of active coatings and films. Results indicate that LS and LO were most suitable for the application studied and differences between them were attributed to a higher degree of laccase-catalyzed cross-linking of LS than of LO. Inclusion in active-packaging systems offers a new way to utilize some types of lignin derivatives from biorefining processes.

Keywords
Lignin derivatives, Laccase, Coating, Film, Oxygen scavenger
National Category
Biological Sciences
Identifiers
urn:nbn:se:umu:diva-90798 (URN)10.1186/1754-1611-8-1 (DOI)000335792000001 ()2-s2.0-84891670496 (Scopus ID)
Available from: 2014-10-09 Created: 2014-07-01 Last updated: 2024-07-02Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0001-8784-9696

Search in DiVA

Show all publications