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Mei, Ya-Fang
Alternative names
Publications (10 of 32) Show all publications
Zhao, H., Wang, M., Muthelo, P., Löf, L., Sterky, F., Gallini, R., . . . Landegren, U. (2022). Detection of SARS-CoV-2 antibodies in serum and dried blood spot samples of vaccinated individuals using a sensitive homogeneous proximity extension assay. New Biotechnology, 72, 139-148
Open this publication in new window or tab >>Detection of SARS-CoV-2 antibodies in serum and dried blood spot samples of vaccinated individuals using a sensitive homogeneous proximity extension assay
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2022 (English)In: New Biotechnology, ISSN 1871-6784, E-ISSN 1876-4347, Vol. 72, p. 139-148Article in journal (Refereed) Published
Abstract [en]

A homogeneous PCR-based assay for sensitive and specific detection of antibodies in serum or dried blood spots (DBS) is presented and the method is used to monitor individuals infected with or vaccinated against SARS-CoV-2. Detection probes were prepared by conjugating the recombinant spike protein subunit 1 (S1), containing the receptor binding domain (RBD) of SARS-CoV-2, to each of a pair of specific oligonucleotides. The same was done for the nucleocapsid protein (NP). Upon incubation with serum or DBS samples, the bi- or multivalency of the antibodies (IgG, IgA or IgM) brings pairs of viral proteins with their conjugated oligonucleotides in proximity, allowing the antibodies to be detected by a modified proximity extension assay (PEA). Anti-S1 and anti-NP antibodies could be detected simultaneously from one incubation reaction. This Antibody PEA (AbPEA) test uses only 1 µl of neat or up to 100,000-fold diluted serum or one ø1.2 mm disc cut from a DBS. All 100 investigated sera and 21 DBS collected prior to the COVID-19 outbreak were negative, demonstrating a 100% specificity. The area under the curve, as evaluated by Receiver Operating Characteristic (ROC) analysis reached 0.998 (95%CI: 0.993–1) for samples taken from 11 days after symptoms onset. The kinetics of antibody responses were monitored after a first and second vaccination using serially collected DBS from 14 individuals. AbPEA offers highly specific and sensitive solution-phase antibody detection without requirement for secondary antibodies, no elution step when using DBS sample in a simple procedure that lends itself to multiplex survey of antibody responses.

Place, publisher, year, edition, pages
Elsevier, 2022
Keywords
Finger-prick dried blood spot, Homogenous serological assay, Multiplex immunoassay, PCR-based antibody detection, Proximity extension assay, SARS-CoV-2 antibody
National Category
Infectious Medicine
Identifiers
urn:nbn:se:umu:diva-201459 (URN)10.1016/j.nbt.2022.11.004 (DOI)000896515300004 ()36423830 (PubMedID)2-s2.0-85142674902 (Scopus ID)
Funder
Knut and Alice Wallenberg Foundation, 2020.0182Swedish Foundation for Strategic Research, SB16-0046Swedish Research Council, 2020-02258
Available from: 2022-12-06 Created: 2022-12-06 Last updated: 2023-09-05Bibliographically approved
Jiang, H. & Mei, Y.-F. (2021). [RETRACTED] Sars–cov–2 spike impairs dna damage repair and inhibits V(D)J recombination in vitro. Viruses, 13(10), Article ID 2056.
Open this publication in new window or tab >>[RETRACTED] Sars–cov–2 spike impairs dna damage repair and inhibits V(D)J recombination in vitro
2021 (English)In: Viruses, E-ISSN 1999-4915, Vol. 13, no 10, article id 2056Article in journal (Refereed) Published
Abstract [en]

Paper retracted.

Place, publisher, year, edition, pages
MDPI, 2021
Keywords
DNA damage repair, SARS–CoV–2, Spike, V(D)J recombination, Vaccine
National Category
Infectious Medicine Immunology in the medical area
Identifiers
urn:nbn:se:umu:diva-188903 (URN)10.3390/v13102056 (DOI)000714086600001 ()34696485 (PubMedID)2-s2.0-85117274155 (Scopus ID)
Note

Retraction: Jiang, Hui, and Ya-Fang Mei. "Retraction: Jiang, H.; Mei, Y.-F. SARS-CoV-2 Spike Impairs DNA Damage Repair and Inhibits V(D)J Recombination In Vitro. Viruses 2021, 13, 2056". Viruses 2022;14,5:1011. DOI: 10.3390/v14051011

Available from: 2021-10-26 Created: 2021-10-26 Last updated: 2024-01-17Bibliographically approved
Gokumakulapalle, M., Wang, L. & Mei, Y.-F. (2021). Susceptibility of Dog, Hamster, and Mouse Cells to the Replication-Competent Adenovirus 11p E1/E3 Green Fluorescence Protein Vector Has Implications for the Selection of Animal Vaccine Models. Frontiers in Microbiology, 12, Article ID 698999.
Open this publication in new window or tab >>Susceptibility of Dog, Hamster, and Mouse Cells to the Replication-Competent Adenovirus 11p E1/E3 Green Fluorescence Protein Vector Has Implications for the Selection of Animal Vaccine Models
2021 (English)In: Frontiers in Microbiology, E-ISSN 1664-302X, Vol. 12, article id 698999Article in journal (Refereed) Published
Abstract [en]

Human adenovirus (Ad)-vectored vaccines require viruses that can internalize into host cells and express the vaccine antigen. Evaluation of the expressed antigen in animal cells is a critical step in preclinical trials of viral vaccines. Due to the species specificity of Ads, it is difficult to find a suitable animal model. Thus, in this study, we compared the efficacy of Ad 11 prototype (Ad11p)-mediated green fluorescence protein (GFP) expression in cell lines of dog (MDCK), hamster (CHO), and mouse (McCoy and C127). Although these cell lines did not express the known primary cellular receptors for Ad11p virus infection (i.e., CD46), Ad11pE1GFP could infect and express GFP with various efficacies. For instance, it manifested relatively higher GFP expression in MDCK than in CHO, McCoy, and C127. However, infection leading to efficient viral release was not observed in any of the studied cell lines. The apparent differences were attributed to particularities of mouse and hamster cell lines, which might have led to the repression of viral DNA synthesis and to the low level of GFP expression mediated by Ad11pe3GFP. Moreover, our results revealed that undetectable hexon protein hampered the assembly of virus particles in CHO and MDCK cells. Ad11p differed from Ad5 in the ability for viral DNA synthesis when infecting CHO cells. Although a defective Ad has been successfully developed for SARS-CoV-2 vaccines in clinical applications, it has been difficult to generate one that can be used as an oral SARS-CoV-2 vaccine. Fortunately, our replication-competent Ad 11p vector might solve this problem. Regarding the use of Ad-vector candidates for vaccine purposes, this study demonstrates the selection of animal cell lines and determination of suitable virus doses in in vitro experiments.

Place, publisher, year, edition, pages
Frontiers Media S.A., 2021
Keywords
animal cells, cytotoxicity, DNA replication, E1/E3 vector, GFP expression, QPCR assay, RCAd11pGFP, susceptibility
National Category
Microbiology in the medical area Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
urn:nbn:se:umu:diva-189618 (URN)10.3389/fmicb.2021.698999 (DOI)000717596800001 ()2-s2.0-85118803323 (Scopus ID)
Available from: 2021-11-17 Created: 2021-11-17 Last updated: 2024-01-17Bibliographically approved
Wu, H. & Mei, Y.-F. (2019). An oncolytic adenovirus 11p vector expressing adenovirus death protein in the E1 region showed significant apoptosis and tumour-killing ability in metastatic prostate cells. Oncotarget, 10(20), 1957-1974
Open this publication in new window or tab >>An oncolytic adenovirus 11p vector expressing adenovirus death protein in the E1 region showed significant apoptosis and tumour-killing ability in metastatic prostate cells
2019 (English)In: Oncotarget, E-ISSN 1949-2553, Vol. 10, no 20, p. 1957-1974Article in journal (Refereed) Published
Abstract [en]

The usefulness for cancer therapy of replication-competent adenoviral vectors expressing therapeutic genes from the E3 region has been evaluated, but few reports have described replication-competent adenoviruses with insertions at the E1 region in the full viral genome. We investigated in different prostate cancer cells the oncolytic efficacy of the replication-competent adenovirus 11p vectors expressing adenovirus death (RCAd11pADP) and red fluorescence (RCAd11pRFP) proteins from the upstream E1 region. ADP/RFP gene expression was 2-3 logs higher in PC3 and DU145 cells than in LNCaP and RWPE-1 cells. E1A protein expression in PC3 and DU145 cells was notably increased after infection with the RCAd11pADP or RCAd11pRFP vector compared with the Ad11pwt virus. Toxicity assays revealed 2-5-fold greater oncolytic effects of RCAd11pADP compared to Ad11pwt. Although all three viruses suppressed subcutaneous PC3 tumour growth in nude mice, RCAd11pRFP had greater oncolytic effects than did the Ad11pwt virus, and RCAd11pADP exhibited significant anti-tumour effects via apoptosis in a xenograft model. Interestingly, the apoptosis triggered by RCAd11pADP was markedly enhanced in comparison to that by the vector expressing ADP from E3 region. Taken together, our findings suggest that RCAd11pADP can potentially be used for the treatment of prostate metastases in clinical settings.

Place, publisher, year, edition, pages
Impact Journals, LLC, 2019
Keywords
apoptosis, expressing adenovirus death protein, in vitro and in vivo, oncolytic adenovirus 11p vector, prostate tumour treatment
National Category
Microbiology in the medical area
Identifiers
urn:nbn:se:umu:diva-185609 (URN)10.18632/oncotarget.26754 (DOI)30956777 (PubMedID)2-s2.0-85062759875 (Scopus ID)
Funder
Swedish Cancer Society, 2011/872
Available from: 2021-07-01 Created: 2021-07-01 Last updated: 2024-01-17Bibliographically approved
Jing, S., Zhang, J., Cao, M., Liu, M., Yan, Y., Zhao, S., . . . Zhang, Q. (2019). Household Transmission of Human Adenovirus Type 55 in Case of Fatal Acute Respiratory Disease [Letter to the editor]. Emerging Infectious Diseases, 25(9), 1756-1758
Open this publication in new window or tab >>Household Transmission of Human Adenovirus Type 55 in Case of Fatal Acute Respiratory Disease
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2019 (English)In: Emerging Infectious Diseases, ISSN 1080-6040, E-ISSN 1080-6059, Vol. 25, no 9, p. 1756-1758Article in journal, Letter (Refereed) Published
Abstract [en]

We identified a case of fatal acute respiratory disease from household transmission of human adenovirus type 55 (HAdV-55) in Anhui Province, China. Computed tomography showed severe pneumonia. Comparative genomic analysis of HAdV-55 indicated the virus possibly originated in Shanxi Province, China. More attention should be paid to highly contagious HAdV-55.

National Category
Infectious Medicine
Identifiers
urn:nbn:se:umu:diva-163658 (URN)10.3201/eid2509.181937 (DOI)000482626000025 ()31441750 (PubMedID)2-s2.0-85071413556 (Scopus ID)
Available from: 2019-10-21 Created: 2019-10-21 Last updated: 2023-03-24Bibliographically approved
Keib, A., Mei, Y.-F., Cicin-Sain, L., Busch, D. H. & Dennehy, K. M. (2019). Measuring Antiviral Capacity of T Cell Responses to Adenovirus. Journal of Immunology, 202(2), 618-624
Open this publication in new window or tab >>Measuring Antiviral Capacity of T Cell Responses to Adenovirus
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2019 (English)In: Journal of Immunology, ISSN 0022-1767, E-ISSN 1550-6606, Vol. 202, no 2, p. 618-624Article in journal (Refereed) Published
Abstract [en]

Adenoviruses are a major cause of infectious mortality in children following allogeneic hematopoietic stem cell transplantation, with adoptive transfer of adenovirus-specific T cells being an effective therapeutic approach. We have previously shown that T cells specific for the peptide epitope LTDLGQNLLY were protective. In this study, we aimed to establish a viral dissemination assay to measure the antiviral capacity of T cells specific for this and other peptide epitopes in an infectious setting. We used replication-competent adenovirus 11 (Ad11pGFP) and adenovirus 5 containing adenovirus 35 fiber (Ad5F35GFP) viruses and T cells specific for HLA-A*01-restricted LTDLGQNLLY, HLA-B*07-restricted KPYSGTAYNAL, and HLA-A*02-restricted LLDQLIEEV peptide epitopes. T cells in PBMC from healthy donors were expanded with peptide and IL-2 or treated with IL-2 alone to serve as nonstimulated control cells, and then these expanded or nonstimulated CD8(+) cells were purified and cocultured with autologous monocytes infected with adenovirus at low multiplicity of infection. After 3 d, the number of infected GFP(+) monocytes and, hence, viral dissemination was quantified by flow cytometry. T cells expanded with LTDLGQNLLY peptide from multiple HLA-A*01(+) donors prevented adenovirus dissemination, and nonstimulated T cells did not prevent dissemination, thus, indicating that LTDLGQNLLY-specific T cells have high antiviral capacity. Similarly, expanded KPYSGTAYNAL- and LLDQLIEEV-specific T cells could prevent viral dissemination. However, the frequency of expanded T cells specific for these last two epitopes was variable between donors with consequent variable prevention of adenoviral dissemination. Taken together, we demonstrate that T cells specific for three peptide epitopes, from both structural and nonstructural proteins, can prevent adenoviral dissemination and provide a novel method to measure the antiviral capacity of adenovirus-specific T cell responses.

Place, publisher, year, edition, pages
American Association of Immunologists, 2019
National Category
Immunology in the medical area
Identifiers
urn:nbn:se:umu:diva-155759 (URN)10.4049/jimmunol.1801003 (DOI)000455041800032 ()30530481 (PubMedID)2-s2.0-85059929900 (Scopus ID)
Available from: 2019-01-28 Created: 2019-01-28 Last updated: 2023-03-23Bibliographically approved
Niittykoski, M., zu Fraunberg, M. v., Martikainen, M., Rauramaa, T., Immonen, A., Koponen, S., . . . Hinkkanen, A. (2017). Immunohistochemical Characterization and Sensitivity to Human Adenovirus Serotypes 3, 5, and 11p of New Cell Lines Derived from Human Diffuse Grade II to IV Gliomas. Translational Oncology, 10(5), 772-779
Open this publication in new window or tab >>Immunohistochemical Characterization and Sensitivity to Human Adenovirus Serotypes 3, 5, and 11p of New Cell Lines Derived from Human Diffuse Grade II to IV Gliomas
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2017 (English)In: Translational Oncology, ISSN 1944-7124, E-ISSN 1936-5233, Vol. 10, no 5, p. 772-779Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Oncolytic adenoviruses show promise in targeting gliomas because they do not replicate in normal brain cells. However, clinical responses occur only in a subset of patients. One explanation could be the heterogenic expression level of virus receptors. Another contributing factor could be variable activity of tumor antiviral defenses in different glioma subtypes. METHODS: We established a collection of primary low-passage cell lines from different glioma subtypes (3 glioblastomas, 3 oligoastrocytomas, and 2 oligodendrogliomas) and assessed them for receptor expression and sensitivity to human adenovirus (HAd) serotypes 3, 5, and 11p. To gauge the impact of antiviral defenses, we also compared the infectivity of the oncolytic adenoviruses in interferon (IFN)-pretreated cells with IFN-sensitive Semliki Forest virus (SFV). RESULTS: Immunostaining revealed generally low expression of HAd5 receptor CAR in both primary tumors and derived cell lines. HAd11p receptor CD46 levels were maintained at moderate levels in both primary tumor samples and derived cell lines. HAd3 receptor DSG-2 was reduced in the cell lines compared to the tumors. Yet, at equal multiplicities of infection, the oncolytic potency of HAd5 in vitro in tumor-derived cells was comparable to HAd11p, whereas HAd3 lysed fewer cells than either of the other two HAd serotypes in 72 hours. IFN blocked replication of SFV, while HAds were rather unaffected. CONCLUSIONS: Adenovirus receptor levels on glioma-derived cell lines did not correlate with infection efficacy and may not be a relevant indicator of clinical oncolytic potency. Adenovirus receptor analysis should be preferentially performed on biopsies obtained perioperatively.

Place, publisher, year, edition, pages
ELSEVIER SCIENCE INC, 2017
National Category
Neurology
Identifiers
urn:nbn:se:umu:diva-140454 (URN)10.1016/j.tranon.2017.07.002 (DOI)000411278400009 ()28797937 (PubMedID)2-s2.0-85029855503 (Scopus ID)
Available from: 2017-10-26 Created: 2017-10-26 Last updated: 2023-03-23Bibliographically approved
Wu, H. D. & Mei, Y.-F. (2017). Replication-competent adenovirus 11p vector armed with ADP gene at E1 region significantly improved tumour-killing effect on metastatic prostate cells in vitro and in vivo. Paper presented at European-Society-of-Gene-and-Cell-Therapy (ESCGT) Congress, 2017, Berlin, GERMANY. Human Gene Therapy, 28(12), A29-A29
Open this publication in new window or tab >>Replication-competent adenovirus 11p vector armed with ADP gene at E1 region significantly improved tumour-killing effect on metastatic prostate cells in vitro and in vivo
2017 (English)In: Human Gene Therapy, ISSN 1043-0342, E-ISSN 1557-7422, Vol. 28, no 12, p. A29-A29Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
Mary Ann Liebert, 2017
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
urn:nbn:se:umu:diva-143941 (URN)000418410700083 ()
Conference
European-Society-of-Gene-and-Cell-Therapy (ESCGT) Congress, 2017, Berlin, GERMANY
Note

Meeting Abstract: P042

Available from: 2018-01-15 Created: 2018-01-15 Last updated: 2018-06-09Bibliographically approved
Mei, Y.-F., Wu, H., Hultenby, K. & Silver, J. (2016). Complete replication-competent adenovirus 11p vectors with E1 or E3 insertions show improved heat stability. Virology, 497, 198-210
Open this publication in new window or tab >>Complete replication-competent adenovirus 11p vectors with E1 or E3 insertions show improved heat stability
2016 (English)In: Virology, ISSN 0042-6822, E-ISSN 1096-0341, Vol. 497, p. 198-210Article in journal (Refereed) Published
Abstract [en]

Conventional adenovirus vectors harboring E1 or E3 deletions followed by the insertion of an exogenous gene show considerably reduced virion stability. Here, we report strategies to generate complete replication-competent Ad11p(RCAd11p) vectors that overcome the above disadvantage. A GFP cassette was successfully introduced either upstream of E1A or in the E3A region. The resulting vectors showed high expression levels of the hexon and E1genes and also strongly induced the cytopathic effect in targeted cells. When harboring oversized genomes, the RCAd11pE1 and RCAd11pE3 vectors showed significantly improved heat stability in comparison to Ad11pwt; of the three, RCAd11pE3 was the most tolerant to heat treatment. Electron microscopy showed that RCAd11pE3, RCAd11pE1, Ad11pwt, and Ad11pE1 Delmanifested dominant, moderate, minimum, or no full virus particles after heat treatment at 47°C for 5 h. Our results demonstrated that both genome size and the insertion site in the viral genome affect virion stability.

Place, publisher, year, edition, pages
Elsevier, 2016
Keywords
Ad11p vectors, Kinetics, Infection, Morphology, Improved stability
National Category
Immunology in the medical area
Identifiers
urn:nbn:se:umu:diva-126737 (URN)10.1016/j.virol.2016.07.026 (DOI)000383922200020 ()27494367 (PubMedID)2-s2.0-84980351615 (Scopus ID)
Available from: 2016-10-20 Created: 2016-10-13 Last updated: 2023-03-24Bibliographically approved
Gokumakulapalle, M. & Mei, Y.-F. (2016). Replication-competent human adenovirus 11p vectors can propagate in Vero cells. Virology, 495, 42-51
Open this publication in new window or tab >>Replication-competent human adenovirus 11p vectors can propagate in Vero cells
2016 (English)In: Virology, ISSN 0042-6822, E-ISSN 1096-0341, Vol. 495, p. 42-51Article in journal (Refereed) Published
Abstract [en]

The use of continuous cell lines derived from the African green monkey kidney (AGMK) has led to major advances in virus vaccine development. However, to date, these cells have not been used to facilitate the creation of human adenoviruses because most human adenoviruses undergo abortive infections in them. Here, we report the susceptibility of AGMK-derived cells to adenovirus lip (Ad11p) infection. First, we showed that CD46 molecules, which act as receptors for Ad11p, are expressed in AGMK cells. We then monitored Ad11p replication by measuring GFP expression as an indicator of viral transcription. We found that AGMK-derived cells were as capable as carcinoma cells at propagating full-length replication competent Ad11p (RCAd11p) DNA. Of the AGMK cell lines tested, Vero cells had the greatest capacity for adenovirus production. Thus, AGMK cells can be used to evaluate RCAd11p-mediated gene delivery, and Vero cells can be used for the production of RCAd11pGFP vectors at relatively high yields.

Keywords
Propagate, RCAd11pGFP vectors, Vero cells, LSERT C, 1984, VIRUS RESEARCH, V1, P365, FER C, 1990, JOURNAL OF VIROLOGY, V64, P3661
National Category
Immunology
Identifiers
urn:nbn:se:umu:diva-124213 (URN)10.1016/j.virol.2016.04.029 (DOI)000378659900005 ()27176913 (PubMedID)2-s2.0-84965136471 (Scopus ID)
Available from: 2016-08-01 Created: 2016-07-28 Last updated: 2023-03-24Bibliographically approved
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