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Springer Engdahl, CeciliaORCID iD iconorcid.org/0000-0002-7264-0125
Alternative names
Publications (10 of 14) Show all publications
Springer Engdahl, C., Caragata, E. P., Tavadia, M. & Dimopoulos, G. (2023). Chromobacterium biopesticide exposure does not select for resistance in Aedes mosquitoes. mBio, 14(2), Article ID e00480-23.
Open this publication in new window or tab >>Chromobacterium biopesticide exposure does not select for resistance in Aedes mosquitoes
2023 (English)In: mBio, ISSN 2161-2129, E-ISSN 2150-7511, Vol. 14, no 2, article id e00480-23Article in journal (Refereed) Published
Abstract [en]

Developing effective tools to control mosquito populations is essential for reducing the incidence of diseases like malaria and dengue. Biopesticides of microbial origin are a rich, underexplored source of mosquitocidal compounds. We previously developed a biopesticide from the bacterium Chromobacterium sp. Panama that rapidly kills vector mosquito larvae, including Aedes aegypti and Anopheles gambiae. Here, we demonstrate that two independent Ae. aegypti colonies exposed to a sublethal dose of that biopesticide over consecutive generations persistently exhibited high mortality and developmental delays, indicating that resistance did not develop during the study period. Critically, the descendants of biopesticide-exposed mosquitoes experienced decreased longevity and did not display increased susceptibility to dengue virus or decreased susceptibility to common chemical insecticides. Through RNA sequencing, we observed no link between biopesticide exposure and the increased activity of xenobiotic metabolism and detoxification genes typically associated with insecticide resistance. These findings indicate that the Chromobacterium biopesticide is an exciting, emerging mosquito control tool.

IMPORTANCE: Vector control is an essential part of mitigating diseases caused by pathogens that mosquitoes spread. Modern vector control is highly reliant on using synthetic insecticides to eliminate mosquito populations before they can cause disease. However, many of these populations have become resistant to commonly used insecticides. There is a strong need to explore alternative vector control strategies that aim to mitigate disease burden. Biopesticides, insecticides of biological origin, can have unique mosquitocidal activities, meaning they can effectively kill mosquitoes that are already resistant to other insecticides. We previously developed a highly effective mosquito biopesticide from the bacterium Chromobacterium sp. Csp_P. Here, we investigate whether exposure to a sublethal dose of this Csp_P biopesticide over 9 to 10 generations causes resistance to arise in Aedes aegypti mosquitoes. We find no evidence of resistance at the physiological or molecular levels, confirming that the Csp_P biopesticide is a highly promising new tool for controlling mosquito populations.

Keywords
mosquito, Aedes aegypti, dengue, biopesticide, Chromobacterium Csp_P, selection, insecticide resistance, RNA-Seq, fitness
National Category
Public Health, Global Health, Social Medicine and Epidemiology Microbiology
Identifiers
urn:nbn:se:umu:diva-208412 (URN)10.1128/mbio.00480-23 (DOI)000963207300001 ()37017525 (PubMedID)2-s2.0-85153898605 (Scopus ID)
Funder
Swedish Research Council, 2018-00334
Available from: 2023-05-23 Created: 2023-05-23 Last updated: 2023-05-24Bibliographically approved
Mutsaers, M., Engdahl, C., Wilkman, L., Ahlm, C., Evander, M. & Lwande, O. W. (2023). Vector competence of Anopheles stephensi for O'nyong-nyong virus: a risk for global virus spread. Parasites & Vectors, 16(1), Article ID 133.
Open this publication in new window or tab >>Vector competence of Anopheles stephensi for O'nyong-nyong virus: a risk for global virus spread
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2023 (English)In: Parasites & Vectors, E-ISSN 1756-3305, Vol. 16, no 1, article id 133Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: O'nyong-nyong virus (ONNV) is a mosquito-borne alphavirus causing sporadic outbreaks of febrile illness with rash and polyarthralgia. Up to now, ONNV has been restricted to Africa and only two competent vectors have been found, Anopheles gambiae and An. funestus, which are also known malaria vectors. With globalization and invasive mosquito species migrating to ONNV endemic areas, there is a possible risk of introduction of the virus to other countries and continents. Anopheles stephensi, is closely related to An. gambiae and one of the invasive mosquito species of Asian origin that is now present in the Horn of Africa and spreading further east. We hypothesize that An. stephensi, a known primary urban malaria vector, may also serve as a new possible vector for ONNV.

METHODS: One-week-old female adult An. stephensi were exposed to ONNV-infected blood, and the vector competence for ONNV, i.e. infection rates (IRs), dissemination rates (DRs), transmission rates (TRs), dissemination efficiency (DEs) and transmission efficiency (TEs), were evaluated. Infection (IRs), dissemination efficiency (DEs) and transmission efficiency (TEs) were determined. Detection of ONNV RNA was analysed by RT-qPCR in the thorax and abdomen, head, wings, legs and saliva of the infected mosquitoes at four different time points, day 7, 14, 21 and 28 after blood meal. Infectious virus in saliva was assessed by infection of Vero B4 cells.

RESULTS: The mean mortality across all sampling times was 27.3% (95 confidence interval [CI] 14.7-44.2%). The mean rate of infection across all sampling periods was 89.5% (95% CI 70.6-95.9). The mean dissemination rate across sampling intervals was 43.4% (95% CI 24.3-64.2%). The mean TR and TE across all mosquito sampling time intervals were 65.3 (95% CI 28.6-93.5) and 74.6 (95% CI 52.1-89.4). The IR was 100%, 79.3%, 78.6% and 100% respectively at 7, 14, 21 and 28 dpi. The DR was the highest at 7 dpi with 76.0%, followed by 28 dpi at 57.1%, 21 dpi at 27.3% and 14 dpi at the lowest DR of 13.04%. DE was 76%, 13.8%, 25%, 57.1% and TR was 79%, 50%, 57.1% and 75% at 7, 14, 21 and 28 dpi respectively. The TE was the highest at 28 dpi, with a proportion of 85.7%. For 7, 14 and 21 dpi the transmission efficiency was 72.0%, 65.5% and 75.0% respectively.

CONCLUSION: Anopheles stephensi is a competent vector for ONNV and being an invasive species spreading to different parts of the world will likely spread the virus to other regions.

Place, publisher, year, edition, pages
BioMed Central (BMC), 2023
Keywords
Alphavirus, Anopheles stephensi, Arthritis, O’nyong-nyong virus, Vector competence
National Category
Microbiology
Identifiers
urn:nbn:se:umu:diva-206949 (URN)10.1186/s13071-023-05725-0 (DOI)000974116700002 ()37069603 (PubMedID)2-s2.0-85152669362 (Scopus ID)
Available from: 2023-04-27 Created: 2023-04-27 Last updated: 2024-01-17Bibliographically approved
S. Engdahl, C., Tikhe, C. V. & Dimopoulos, G. (2022). Discovery of novel natural products for mosquito control. Parasites & Vectors, 15(1), Article ID 481.
Open this publication in new window or tab >>Discovery of novel natural products for mosquito control
2022 (English)In: Parasites & Vectors, E-ISSN 1756-3305, Vol. 15, no 1, article id 481Article in journal (Refereed) Published
Abstract [en]

Vector control plays a key role in reducing the public health burden of mosquito-borne diseases. Today’s vector control strategies largely rely on synthetic insecticides that can have a negative environmental impact when applied outdoors and often become inefficient because of the mosquitoes’ ability to develop resistance. An alternative and promising approach to circumvent these challenges involves the implementation of insecticides derived from nature (biopesticides) for vector control. Biopesticides can constitute naturally occurring organisms or substances derived from them that have lifespan-shortening effects on disease vectors such as mosquitoes. Here we present the discovery and evaluation of natural product-based biological control agents that can potentially be developed into biopesticides for mosquito control. We screened a natural product collection comprising 390 compounds and initially identified 26 molecules with potential ability to kill the larval stages of the yellow fever mosquito Aedes aegypti, which is responsible for transmitting viruses such as dengue, Zika, chikungunya and yellow fever. Natural products identified as hits in the screen were further evaluated for their suitability for biopesticide development. We show that a selection of the natural product top hits, bactobolin, maytansine and ossamycin, also killed the larval stages of the malaria-transmitting mosquito Anopheles gambiae as well as the adult form of both species. We have further explored the usefulness of crude extracts and preparations from two of the best candidates’ sources (organisms of origin) for mosquitocidal activity, that is extracts from the two bacteria Burkholderia thailandensis and Streptomyces hygroscopicus var. ossamyceticus.

Place, publisher, year, edition, pages
BioMed Central (BMC), 2022
Keywords
Vector control, Biopesticide, Natural products, Mosquito-borne diseases, Aedes aegypti, Anopheles gambiae
National Category
Public Health, Global Health, Social Medicine and Epidemiology Biochemistry and Molecular Biology Microbiology
Identifiers
urn:nbn:se:umu:diva-202106 (URN)10.1186/s13071-022-05594-z (DOI)000901777900001 ()36539851 (PubMedID)2-s2.0-85144306743 (Scopus ID)
Funder
Swedish Research Council, 2018-00334NIH (National Institutes of Health), R21AI146398
Available from: 2023-01-02 Created: 2023-01-02 Last updated: 2024-01-17Bibliographically approved
Accoti, A., Engdahl, C. & Dimopoulos, G. (2021). Discovery of Novel Entomopathogenic Fungi for Mosquito-Borne Disease Control. Frontiers in Fungal Biology, 2, Article ID 637234.
Open this publication in new window or tab >>Discovery of Novel Entomopathogenic Fungi for Mosquito-Borne Disease Control
2021 (English)In: Frontiers in Fungal Biology, E-ISSN 2673-6128, Vol. 2, article id 637234Article in journal (Refereed) Published
Abstract [en]

The increased application of chemical control programs has led to the emergence and spread of insecticide resistance in mosquitoes. Novel environmentally safe control strategies are currently needed for the control of disease vectors. The use of entomopathogenic fungi could be a suitable alternative to chemical insecticides. Currently, Beauveria spp. and Metarhizium spp. are the most widely used entomopathogenic fungi for mosquito control, but increasing the arsenal with additional fungi is necessary to mitigate the emergence of resistance. Entomopathogenic fungi are distributed in a wide range of habitats. We have performed a comprehensive screen for candidate mosquitocidal fungi from diverse outdoor environments in Maryland and Puerto Rico. An initial screening of 22 fungi involving exposure of adult Anopheles gambiae to 2-weeks-old fungal cultures identified five potent pathogenic fungi, one of which is unidentified and the remaining four belonging to the three genera Galactomyces sp., Isaria sp. and Mucor sp. These fungi were then screened against Aedes aegypti, revealing Isaria sp. as a potent mosquito killer. The entomopathogenic effects were confirmed through spore-dipping assays. We also probed further into the killing mechanisms of these fungi and investigated whether the mosquitocidal activities were the result of potential toxic fungus-produced metabolites. Preliminary assays involving the exposure of mosquitoes to sterile filtered fungal liquid cultures showed that Galactomyces sp., Isaria sp. and the unidentified isolate 1 were the strongest producers of factors showing lethality against An. gambiae. We have identified five fungi that was pathogenic for An. gambiae and one for Ae. aegypti, among these fungi, four of them (two strains of Galactomyces sp., Mucor sp., and the unidentified isolate 1) have never previously been described as lethal to insects. Further characterization of these entomopathogenic fungi and their metabolites needs to be done to confirm their potential use in biologic control against mosquitoes.

Place, publisher, year, edition, pages
Frontiers Media S.A., 2021
National Category
Public Health, Global Health, Social Medicine and Epidemiology
Identifiers
urn:nbn:se:umu:diva-189208 (URN)10.3389/ffunb.2021.637234 (DOI)
Funder
Swedish Research Council, 2018-00334
Available from: 2021-11-09 Created: 2021-11-09 Last updated: 2021-11-11Bibliographically approved
Knutsson, S., Engdahl, C., Kumari, R., Forsgren, N., Lindgren, C., Kindahl, T., . . . Linusson, A. (2018). Noncovalent Inhibitors of Mosquito Acetylcholinesterase 1 with Resistance-Breaking Potency. Journal of Medicinal Chemistry, 61(23), 10545-10557
Open this publication in new window or tab >>Noncovalent Inhibitors of Mosquito Acetylcholinesterase 1 with Resistance-Breaking Potency
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2018 (English)In: Journal of Medicinal Chemistry, ISSN 0022-2623, E-ISSN 1520-4804, Vol. 61, no 23, p. 10545-10557Article in journal (Refereed) Published
Abstract [en]

Resistance development in insects significantly threatens the important benefits obtained by insecticide usage in vector control of disease-transmitting insects. Discovery of new chemical entities with insecticidal activity is highly desired in order to develop new insecticide candidates. Here, we present the design, synthesis, and biological evaluation of phenoxyacetamide-based inhibitors of the essential enzyme acetylcholinesterase 1 (AChE1). AChE1 is a validated insecticide target to control mosquito vectors of, e.g., malaria, dengue, and Zika virus infections. The inhibitors combine a mosquito versus human AChE selectivity with a high potency also for the resistance-conferring mutation G122S; two properties that have proven challenging to combine in a single compound. Structure activity relationship analyses and molecular dynamics simulations of inhibitor protein complexes have provided insights that elucidate the molecular basis for these properties. We also show that the inhibitors demonstrate in vivo insecticidal activity on disease-transmitting mosquitoes. Our findings support the concept of noncovalent, selective, and resistance-breaking inhibitors of AChE1 as a promising approach for future insecticide development.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2018
National Category
Medicinal Chemistry
Identifiers
urn:nbn:se:umu:diva-154812 (URN)10.1021/acs.jmedchem.8b01060 (DOI)000453488200014 ()30339371 (PubMedID)2-s2.0-85058504373 (Scopus ID)
Funder
Swedish Research Council, 2014-4218Swedish Research Council, 2014-2636
Available from: 2019-01-07 Created: 2019-01-07 Last updated: 2023-03-23Bibliographically approved
Knutsson, S., Kindahl, T., Engdahl, C., Nikjoo, D., Forsgren, N., Kitur, S., . . . Linusson, A. (2017). N-Aryl-N'-ethyleneaminothioureas effectively inhibit acetylcholinesterase 1 from disease-transmitting mosquitoes. European Journal of Medicinal Chemistry, 134, 415-427
Open this publication in new window or tab >>N-Aryl-N'-ethyleneaminothioureas effectively inhibit acetylcholinesterase 1 from disease-transmitting mosquitoes
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2017 (English)In: European Journal of Medicinal Chemistry, ISSN 0223-5234, E-ISSN 1768-3254, Vol. 134, p. 415-427Article in journal (Refereed) Published
Abstract [en]

Vector control of disease-transmitting mosquitoes by insecticides has a central role in reducing the number of parasitic- and viral infection cases. The currently used insecticides are efficient, but safety concerns and the development of insecticide-resistant mosquito strains warrant the search for alternative compound classes for vector control. Here, we have designed and synthesized thiourea-based compounds as non-covalent inhibitors of acetylcholinesterase 1 (AChE1) from the mosquitoes Anopheles gambiae (An. gambiae) and Aedes aegypti (Ae. aegypti), as well as a naturally occurring resistant-conferring mutant. The N-aryl-N'-ethyleneaminothioureas proved to be inhibitors of AChE1; the most efficient one showed submicromolar potency. Importantly, the inhibitors exhibited selectivity over the human AChE (hAChE), which is desirable for new insecticides. The structure-activity relationship (SAR) analysis of the thioureas revealed that small changes in the chemical structure had a large effect on inhibition capacity. The thioureas showed to have different SAR when inhibiting AChE1 and hAChE, respectively, enabling an investigation of structure-selectivity relationships. Furthermore, insecticidal activity was demonstrated using adult and larvae An. gambiae and Ae. aegypti mosquitoes.

Keywords
Acetylcholinesterase 1, Aedes aegypti, Anopheles gambiae, Insecticides, Thiourea, Vector control
National Category
Organic Chemistry
Identifiers
urn:nbn:se:umu:diva-134612 (URN)10.1016/j.ejmech.2017.03.050 (DOI)000401677500035 ()28433681 (PubMedID)2-s2.0-85017625955 (Scopus ID)
Available from: 2017-05-09 Created: 2017-05-09 Last updated: 2023-03-24Bibliographically approved
Engdahl, C. (2017). Selective inhibition of acetylcholinesterase 1 from disease-transmitting mosquitoes: design and development of new insecticides for vector control. (Doctoral dissertation). Umeå: Umeå University
Open this publication in new window or tab >>Selective inhibition of acetylcholinesterase 1 from disease-transmitting mosquitoes: design and development of new insecticides for vector control
2017 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Acetylcholinesterase (AChE) is an essential enzyme with an evolutionary conserved function: to terminate nerve signaling by rapid hydrolysis of the neurotransmitter acetylcholine. AChE is an important target for insecticides. Vector control by the use of insecticide-based interventions is today the main strategy for controlling mosquito-borne diseases that affect millions of people each year. However, the efficiency of many insecticides is challenged by resistant mosquito populations, lack of selectivity and off-target toxicity of currently used compounds. New selective and resistance-breaking insecticides are needed for an efficient vector control also in the future. In the work presented in this thesis, we have combined structural biology, biochemistry and medicinal chemistry to characterize mosquito AChEs and to develop selective and resistance-breaking inhibitors of this essential enzyme from two disease-transmitting mosquitoes.We have identified small but important structural and functional differences between AChE from mosquitoes and AChE from vertebrates. The significance of these differences was emphasized by a high throughput screening campaign, which made it evident that the evolutionary distant AChEs display significant differences in their molecular recognition. These findings were exploited in the design of new inhibitors. Rationally designed and developed thiourea- and phenoxyacetamide-based non-covalent inhibitors displayed high potency on both wild type and insecticide insensitive AChE from mosquitoes. The best inhibitors showed over 100-fold stronger inhibition of mosquito than human AChE, and proved insecticide potential as they killed both adult and larvae mosquitoes.We show that mosquito and human AChE have different molecular recognition and that non-covalent selective inhibition of AChE from mosquitoes is possible. We also demonstrate that inhibitors can combine selectivity with sub-micromolar potency for insecticide resistant AChE.

Place, publisher, year, edition, pages
Umeå: Umeå University, 2017. p. 67
Keywords
acetylcholinesterase, non-covalent inhibitor, vector control, insecticide, mosquito, vector-borne disease, high throughput screening, rational design
National Category
Biochemistry and Molecular Biology
Research subject
Biochemistry; computational linguistics
Identifiers
urn:nbn:se:umu:diva-134625 (URN)978-91-7601-723-4 (ISBN)
Public defence
2017-06-02, KB.E3.03 (stora hörsalen), KBC-huset, Umeå, 09:00 (English)
Opponent
Supervisors
Available from: 2017-05-12 Created: 2017-05-10 Last updated: 2018-06-09Bibliographically approved
Engdahl, C., Knutsson, S., Ekström, F. & Linusson, A. (2016). Discovery of selective inhibitors targeting acetylcholinesterase 1 from disease-transmitting mosquitoes. Journal of Medicinal Chemistry, 59(20), 9409-9421
Open this publication in new window or tab >>Discovery of selective inhibitors targeting acetylcholinesterase 1 from disease-transmitting mosquitoes
2016 (English)In: Journal of Medicinal Chemistry, ISSN 0022-2623, E-ISSN 1520-4804, Vol. 59, no 20, p. 9409-9421Article in journal (Other academic) Published
Abstract [en]

Vector control of disease-transmitting mosquitoes is increasingly important due to the re-emergence and spread of infections such as malaria and dengue. We have conducted a high throughput screen (HTS) of 17,500 compounds for inhibition of the essential AChE1 enzymes from the mosquitoes Anopheles gambiae and Aedes aegypti. In a differential HTS analysis including the human AChE, several structurally diverse, potent, and selective noncovalent AChE1 inhibitors were discovered. For example, a phenoxyacetamide-based inhibitor was identified with a 100-fold selectivity for the mosquito over the human enzyme. The compound also inhibited a resistance conferring mutant of AChE1. Structure-selectivity relationships could be proposed based on the enzymes' 3D structures; the hits' selectivity profiles appear to be linked to differences in two loops that affect the structure of the entire active site. Noncovalent inhibitors of AChE1, such as the ones presented here, provide valuable starting points toward insecticides and are complementary to existing and new covalent inhibitors.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2016
National Category
Organic Chemistry
Identifiers
urn:nbn:se:umu:diva-119925 (URN)10.1021/acs.jmedchem.6b00967 (DOI)000386641300010 ()2-s2.0-84994018423 (Scopus ID)
Note

Originally included in thesis in manuscript form.

Available from: 2016-05-03 Created: 2016-05-02 Last updated: 2023-03-23Bibliographically approved
Engdahl, C., Knutsson, S., Fredriksson, S.-Å., Linusson, A., Bucht, G. & Ekström, F. (2015). Acetylcholinesterases from the Disease Vectors Aedes aegypti and Anopheles gambiae: Functional Characterization and Comparisons with Vertebrate Orthologues. PLOS ONE, 10(10), Article ID e0138598.
Open this publication in new window or tab >>Acetylcholinesterases from the Disease Vectors Aedes aegypti and Anopheles gambiae: Functional Characterization and Comparisons with Vertebrate Orthologues
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2015 (English)In: PLOS ONE, E-ISSN 1932-6203, Vol. 10, no 10, article id e0138598Article in journal (Refereed) Published
Abstract [en]

Mosquitoes of the Anopheles (An.) and Aedes (Ae.) genus are principal vectors of human diseases including malaria, dengue and yellow fever. Insecticide-based vector control is an established and important way of preventing transmission of such infections. Currently used insecticides can efficiently control mosquito populations, but there are growing concerns about emerging resistance, off-target toxicity and their ability to alter ecosystems. A potential target for the development of insecticides with reduced off-target toxicity is the cholinergic enzyme acetylcholinesterase (AChE). Herein, we report cloning, baculoviral expression and functional characterization of the wild-type AChE genes (ace-1) from An. gambiae and Ae. aegypti, including a naturally occurring insecticide-resistant (G119S) mutant of An. gambiae. Using enzymatic digestion and liquid chromatography-tandem mass spectrometry we found that the secreted proteins were post-translationally modified. The Michaelis-Menten constants and turnover numbers of the mosquito enzymes were lower than those of the orthologous AChEs from Mus musculus and Homo sapiens. We also found that the G119S substitution reduced the turnover rate of substrates and the potency of selected covalent inhibitors. Furthermore, non-covalent inhibitors were less sensitive to the G119S substitution and differentiate the mosquito enzymes from corresponding vertebrate enzymes. Our findings indicate that it may be possible to develop selective non-covalent inhibitors that effectively target both the wild-type and insecticide resistant mutants of mosquito AChE.

National Category
Other Chemistry Topics
Identifiers
urn:nbn:se:umu:diva-110996 (URN)10.1371/journal.pone.0138598 (DOI)000362511000010 ()26447952 (PubMedID)2-s2.0-84949033885 (Scopus ID)
Funder
Swedish Research Council
Available from: 2015-11-17 Created: 2015-11-02 Last updated: 2023-03-24Bibliographically approved
Bergqvist, J., Forsman, O., Larsson, P., Näslund, J., Lilja, T., Engdahl, C., . . . Bucht, G. (2015). Detection and Isolation of Sindbis Virus from Mosquitoes Captured During an Outbreak in Sweden, 2013. Vector Borne and Zoonotic Diseases, 15(2), 133-140
Open this publication in new window or tab >>Detection and Isolation of Sindbis Virus from Mosquitoes Captured During an Outbreak in Sweden, 2013
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2015 (English)In: Vector Borne and Zoonotic Diseases, ISSN 1530-3667, E-ISSN 1557-7759, Vol. 15, no 2, p. 133-140Article in journal (Refereed) Published
Abstract [en]

Mosquito-borne alphaviruses have the potential to cause large outbreaks throughout the world. Here we investigated the causative agent of an unexpected Sindbis virus (SINV) outbreak during August-September, 2013, in a previously nonendemic region of Sweden. Mosquitoes were collected using carbon dioxide-baited CDC traps at locations close to human cases. The mosquitoes were initially screened as large pools by SINV-specific quantitative RT-PCR, and the SINV-positive mosquitoes were species determined by single-nucleotide polymorphism (SNP) analysis, followed by sequencing the barcoding region of the cytochrome oxidase I gene. The proportion of the collected mosquitoes was determined by a metabarcoding strategy. By using novel strategies for PCR screening and genetic typing, a new SINV strain, Lovanger, was isolated from a pool of 1600 mosquitoes composed of Culex, Culiseta, and Aedes mosquitoes as determined by metabarcoding. The SINV-positive mosquito Culiseta morsitans was identified by SNP analysis and sequencing. After whole-genome sequencing and phylogenetic analysis, the SINV Lovanger isolate was shown to be most closely similar to recent Finnish SINV isolates. In conclusion, within a few weeks, we were able to detect and isolate a novel SINV strain and identify the mosquito vector during a sudden SINV outbreak.

Keywords
Barcoding, Mosquito, Isolation, Virus, Sindbis
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:umu:diva-100963 (URN)10.1089/vbz.2014.1717 (DOI)000349682500007 ()25700044 (PubMedID)2-s2.0-84923396181 (Scopus ID)
Available from: 2015-03-18 Created: 2015-03-16 Last updated: 2024-07-02Bibliographically approved
Projects
Identify and explore bacteria-produced mosquitocidal molecules [2018-00334_VR]; Umeå University
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0002-7264-0125

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