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Publications (10 of 52) Show all publications
Pitsili, E., Rodriguez-Trevino, R., Ruiz-Solani, N., Demir, F., Kastanaki, E., Dambire, C., . . . Coll, N. S. (2023). A phloem-localized Arabidopsis metacaspase (AtMC3) improves drought tolerance. New Phytologist, 239, 1281-1299
Open this publication in new window or tab >>A phloem-localized Arabidopsis metacaspase (AtMC3) improves drought tolerance
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2023 (English)In: New Phytologist, ISSN 0028-646X, E-ISSN 1469-8137, Vol. 239, p. 1281-1299Article in journal (Refereed) Published
Abstract [en]

Increasing drought phenomena pose a serious threat to agricultural productivity. Although plants have multiple ways to respond to the complexity of drought stress, the underlying mechanisms of stress sensing and signaling remain unclear. The role of the vasculature, in particular the phloem, in facilitating inter-organ communication is critical and poorly understood. Combining genetic, proteomic and physiological approaches, we investigated the role of AtMC3, a phloem-specific member of the metacaspase family, in osmotic stress responses in Arabidopsis thaliana. Analyses of the proteome in plants with altered AtMC3 levels revealed differential abundance of proteins related to osmotic stress pointing into a role of the protein in water-stress-related responses. Overexpression of AtMC3 conferred drought tolerance by enhancing the differentiation of specific vascular tissues and maintaining higher levels of vascular-mediated transportation, while plants lacking the protein showed an impaired response to drought and inability to respond effectively to the hormone abscisic acid. Overall, our data highlight the importance of AtMC3 and vascular plasticity in fine-tuning early drought responses at the whole plant level without affecting growth or yield.

Place, publisher, year, edition, pages
John Wiley & Sons, 2023
Keywords
abscisic acid, Arabidopsis thaliana, drought, hypoxia, metacaspases, osmotic stress, phloem
National Category
Botany
Identifiers
urn:nbn:se:umu:diva-210685 (URN)10.1111/nph.19022 (DOI)001010896700001 ()37320971 (PubMedID)2-s2.0-85161826198 (Scopus ID)
Available from: 2023-06-26 Created: 2023-06-26 Last updated: 2023-09-22Bibliographically approved
Escamez, S., Robinson, K. M., Luomaranta, M., Gandla, M. L., Mähler, N., Yassin, Z., . . . Tuominen, H. (2023). Genetic markers and tree properties predicting wood biorefining potential in aspen (Populus tremula) bioenergy feedstock. Biotechnology for Biofuels and Bioproducts, 16(1), Article ID 65.
Open this publication in new window or tab >>Genetic markers and tree properties predicting wood biorefining potential in aspen (Populus tremula) bioenergy feedstock
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2023 (English)In: Biotechnology for Biofuels and Bioproducts, E-ISSN 2731-3654, Vol. 16, no 1, article id 65Article in journal (Refereed) Published
Abstract [en]

Background: Wood represents the majority of the biomass on land and constitutes a renewable source of biofuels and other bioproducts. However, wood is recalcitrant to bioconversion, raising a need for feedstock improvement in production of, for instance, biofuels. We investigated the properties of wood that affect bioconversion, as well as the underlying genetics, to help identify superior tree feedstocks for biorefining.

Results: We recorded 65 wood-related and growth traits in a population of 113 natural aspen genotypes from Sweden (https://doi.org/10.5061/dryad.gtht76hrd). These traits included three growth and field performance traits, 20 traits for wood chemical composition, 17 traits for wood anatomy and structure, and 25 wood saccharification traits as indicators of bioconversion potential. Glucose release after saccharification with acidic pretreatment correlated positively with tree stem height and diameter and the carbohydrate content of the wood, and negatively with the content of lignin and the hemicellulose sugar units. Most of these traits displayed extensive natural variation within the aspen population and high broad-sense heritability, supporting their potential in genetic improvement of feedstocks towards improved bioconversion. Finally, a genome-wide association study (GWAS) revealed 13 genetic loci for saccharification yield (on a whole-tree-biomass basis), with six of them intersecting with associations for either height or stem diameter of the trees.

Conclusions: The simple growth traits of stem height and diameter were identified as good predictors of wood saccharification yield in aspen trees. GWAS elucidated the underlying genetics, revealing putative genetic markers for bioconversion of bioenergy tree feedstocks.

Place, publisher, year, edition, pages
BioMed Central (BMC), 2023
Keywords
Bioenergy, Biomass, Biorefining, Feedstock recalcitrance, Forest feedstocks, Saccharification
National Category
Forest Science
Identifiers
urn:nbn:se:umu:diva-206938 (URN)10.1186/s13068-023-02315-1 (DOI)000967835900001 ()2-s2.0-85152632077 (Scopus ID)
Funder
Swedish Research Council Formas, 942-2015-84Swedish Research Council Formas, 2018-01381Knut and Alice Wallenberg Foundation, 2016.0341Knut and Alice Wallenberg Foundation, 2016.0352Vinnova, 2016-00504Bio4Energy
Available from: 2023-04-27 Created: 2023-04-27 Last updated: 2023-11-06Bibliographically approved
Gandla, M. L., Mähler, N., Escamez, S., Skotare, T., Obudulu, O., Möller, L., . . . Jönsson, L. J. (2021). Overexpression of vesicle-associated membrane protein PttVAP27-17 as a tool to improve biomass production and the overall saccharification yields in Populus trees. Biotechnology for Biofuels, 14(1), Article ID 43.
Open this publication in new window or tab >>Overexpression of vesicle-associated membrane protein PttVAP27-17 as a tool to improve biomass production and the overall saccharification yields in Populus trees
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2021 (English)In: Biotechnology for Biofuels, ISSN 1754-6834, E-ISSN 1754-6834, Vol. 14, no 1, article id 43Article in journal (Refereed) Published
Abstract [en]

Background: Bioconversion of wood into bioproducts and biofuels is hindered by the recalcitrance of woody raw material to bioprocesses such as enzymatic saccharification. Targeted modification of the chemical composition of the feedstock can improve saccharification but this gain is often abrogated by concomitant reduction in tree growth.

Results: In this study, we report on transgenic hybrid aspen (Populus tremula × tremuloides) lines that showed potential to increase biomass production both in the greenhouse and after 5 years of growth in the field. The transgenic lines carried an overexpression construct for Populus tremula × tremuloides vesicle-associated membrane protein (VAMP)-associated protein PttVAP27-17 that was selected from a gene-mining program for novel regulators of wood formation. Analytical-scale enzymatic saccharification without any pretreatment revealed for all greenhouse-grown transgenic lines, compared to the wild type, a 20–44% increase in the glucose yield per dry weight after enzymatic saccharification, even though it was statistically significant only for one line. The glucose yield after enzymatic saccharification with a prior hydrothermal pretreatment step with sulfuric acid was not increased in the greenhouse-grown transgenic trees on a dry-weight basis, but increased by 26–50% when calculated on a whole biomass basis in comparison to the wild-type control. Tendencies to increased glucose yields by up to 24% were present on a whole tree biomass basis after acidic pretreatment and enzymatic saccharification also in the transgenic trees grown for 5 years on the field when compared to the wild-type control.

Conclusions: The results demonstrate the usefulness of gene-mining programs to identify novel genes with the potential to improve biofuel production in tree biotechnology programs. Furthermore, multi-omic analyses, including transcriptomic, proteomic and metabolomic analyses, performed here provide a toolbox for future studies on the function of VAP27 proteins in plants.

Place, publisher, year, edition, pages
BioMed Central, 2021
Keywords
Bioprocessing, Growth, Metabolomics, Populus, Proteomics, Transcriptomics, VAMP, VAMP-associated protein, VAP27, Vesicle-associated membrane protein
National Category
Plant Biotechnology
Identifiers
urn:nbn:se:umu:diva-180984 (URN)10.1186/s13068-021-01895-0 (DOI)000620931600002 ()2-s2.0-85100873005 (Scopus ID)
Available from: 2021-03-05 Created: 2021-03-05 Last updated: 2023-11-06Bibliographically approved
Milhinhos, A., Bollhöner, B., Blazquez, M. A., Novak, O., Miguel, C. M. & Tuominen, H. (2020). ACAULIS5 Is Required for Cytokinin Accumulation and Function During Secondary Growth of Populus Trees. Frontiers in Plant Science, 11, Article ID 601858.
Open this publication in new window or tab >>ACAULIS5 Is Required for Cytokinin Accumulation and Function During Secondary Growth of Populus Trees
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2020 (English)In: Frontiers in Plant Science, E-ISSN 1664-462X, Vol. 11, article id 601858Article in journal (Refereed) Published
Abstract [en]

In the primary root and young hypocotyl of Arabidopsis, ACAULIS5 promotes translation of SUPPRESSOR OF ACAULIS51 (SAC51) and thereby inhibits cytokinin biosynthesis and vascular cell division. In this study, the relationships between ACAULIS5, SAC51 and cytokinin biosynthesis were investigated during secondary growth of Populus stems. Overexpression of ACAULIS5 from the constitutive 35S promoter in hybrid aspen (Populus tremula x Populus tremuloides) trees suppressed the expression level of ACAULIS5, which resulted in low levels of the physiologically active cytokinin bases as well as their direct riboside precursors in the transgenic lines. Low ACAULIS5 expression and low cytokinin levels of the transgenic trees coincided with low cambial activity of the stem. ACAULIS5 therefore, contrary to its function in young seedlings in Arabidopsis, stimulates cytokinin accumulation and cambial activity during secondary growth of the stem. This function is not derived from maturing secondary xylem tissues as transgenic suppression of ACAULIS5 levels in these tissues did not influence secondary growth. Interestingly, evidence was obtained for increased activity of the anticlinal division of the cambial initials under conditions of low ACAULIS5 expression and low cytokinin accumulation. We propose that ACAULIS5 integrates auxin and cytokinin signaling to promote extensive secondary growth of tree stems.

Place, publisher, year, edition, pages
Frontiers Media S.A., 2020
Keywords
ACAULIS5, cytokinin, POPACAULIS5, polyamine, Populus tremula × Populus tremuloides, thermospermine, wood development, xylem
National Category
Botany
Identifiers
urn:nbn:se:umu:diva-177731 (URN)10.3389/fpls.2020.601858 (DOI)000593932900001 ()33304375 (PubMedID)2-s2.0-85096938631 (Scopus ID)
Available from: 2020-12-17 Created: 2020-12-17 Last updated: 2024-01-17Bibliographically approved
Escamez, S., André, D., Sztojka, B., Bollhöner, B., Hall, H., Berthet, B., . . . Tuominen, H. (2020). Cell Death in Cells Overlying Lateral Root Primordia Facilitates Organ Growth in Arabidopsis. Current Biology, 30(3), 455-464
Open this publication in new window or tab >>Cell Death in Cells Overlying Lateral Root Primordia Facilitates Organ Growth in Arabidopsis
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2020 (English)In: Current Biology, ISSN 0960-9822, E-ISSN 1879-0445, Vol. 30, no 3, p. 455-464Article in journal (Refereed) Published
Abstract [en]

Plant organ growth is widely accepted to be determined by cell division and cell expansion, but, unlike that in animals, the contribution of cell elimination has rarely been recognized. We investigated this paradigm during Arabidopsis lateral root formation, when the lateral root primordia (LRP) must traverse three overlying cell layers within the parent root. A subset of LRP-overlying cells displayed the induction of marker genes for cell types undergoing developmental cell death, and their cell death was detected by electron, confocal, and light sheet microscopy techniques. LRP growth was delayed in cell-deathdeficient mutants lacking the positive cell death regulator ORESARA1/ANAC092 (ORE1). LRP growth was restored in ore1-2 knockout plants by genetically inducing cell elimination in cells overlying the LRP or by physically killing LRP-overlying cells by ablation with optical tweezers. Our results support that, in addition to previously discovered mechanisms, cell elimination contributes to regulating lateral root emergence.

Place, publisher, year, edition, pages
Elsevier, 2020
National Category
Developmental Biology Other Physics Topics
Identifiers
urn:nbn:se:umu:diva-168335 (URN)10.1016/j.cub.2019.11.078 (DOI)000511287900024 ()31956028 (PubMedID)2-s2.0-85078465553 (Scopus ID)
Projects
Bio4Energy
Funder
Bio4Energy
Available from: 2020-02-24 Created: 2020-02-24 Last updated: 2023-03-24Bibliographically approved
Lakehal, A., Dob, A., Rahneshan, Z., Novak, O., Escamez, S., Alallaq, S., . . . Bellini, C. (2020). ETHYLENE RESPONSE FACTOR 115 integrates jasmonate and cytokinin signaling machineries to repress adventitious rooting in Arabidopsis. New Phytologist, 228, 1611-1626
Open this publication in new window or tab >>ETHYLENE RESPONSE FACTOR 115 integrates jasmonate and cytokinin signaling machineries to repress adventitious rooting in Arabidopsis
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2020 (English)In: New Phytologist, ISSN 0028-646X, E-ISSN 1469-8137, Vol. 228, p. 1611-1626Article in journal (Refereed) Published
Abstract [en]

Adventitious root initiation (ARI) is ade novoorganogenesis program and a key adaptive trait in plants. Several hormones regulate ARI but the underlying genetic architecture that integrates the hormonal crosstalk governing this process remains largely elusive. In this study, we use genetics, genome editing, transcriptomics, hormone profiling and cell biological approaches to demonstrate a crucial role played by the APETALA2/ETHYLENE RESPONSE FACTOR 115 transcription factor. We demonstrate that ERF115 functions as a repressor of ARI by activating the cytokinin (CK) signaling machinery. We also demonstrate thatERF115is transcriptionally activated by jasmonate (JA), an oxylipin-derived phytohormone, which represses ARI in NINJA-dependent and independent manners. Our data indicate that NINJA-dependent JA signaling in pericycle cells blocks early events of ARI. Altogether, our results reveal a previously unreported molecular network involving cooperative crosstalk between JA and CK machineries that represses ARI.

Place, publisher, year, edition, pages
Wiley-Blackwell Publishing Inc., 2020
Keywords
adventitious rooting, AP2, ERF transcription factors, cytokinins, de novo organogenesis, jasmonate
National Category
Botany
Identifiers
urn:nbn:se:umu:diva-174347 (URN)10.1111/nph.16794 (DOI)000556034400001 ()32634250 (PubMedID)2-s2.0-85089083157 (Scopus ID)
Available from: 2020-08-20 Created: 2020-08-20 Last updated: 2023-03-23Bibliographically approved
Zhang, B., Sztojka, B., Escamez, S., Vanholme, R., Hedenström, M., Wang, Y., . . . Tuominen, H. (2020). PIRIN2 suppresses S-type lignin accumulation in a noncell-autonomous manner in Arabidopsis xylem elements. New Phytologist, 225(5), 1923-1935
Open this publication in new window or tab >>PIRIN2 suppresses S-type lignin accumulation in a noncell-autonomous manner in Arabidopsis xylem elements
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2020 (English)In: New Phytologist, ISSN 0028-646X, E-ISSN 1469-8137, Vol. 225, no 5, p. 1923-1935Article in journal (Refereed) Published
Abstract [en]
  • PIRIN (PRN) genes encode cupin domain‐containing proteins that function as transcriptional co‐regulators in humans but that are poorly described in plants. A previous study in xylogenic cell cultures of Zinnia elegans suggested a role for a PRN protein in lignification. This study aimed to identify the function of Arabidopsis (Arabidopsis thaliana) PRN proteins in lignification of xylem tissues.
  • Chemical composition of the secondary cell walls was analysed in Arabidopsis stems and/or hypocotyls by pyrolysis–gas chromatography/mass spectrometry, 2D‐nuclear magnetic resonance and phenolic profiling. Secondary cell walls of individual xylem elements were chemotyped by Fourier transform infrared and Raman microspectroscopy.
  • Arabidopsis PRN2 suppressed accumulation of S‐type lignin in Arabidopsis stems and hypocotyls. PRN2 promoter activity and PRN2:GFP fusion protein were localised specifically in cells next to the vessel elements, suggesting a role for PRN2 in noncell‐autonomous lignification of xylem vessels. Accordingly, PRN2 modulated lignin chemistry in the secondary cell walls of the neighbouring vessel elements.
  • These results indicate that PRN2 suppresses S‐type lignin accumulation in the neighbourhood of xylem vessels to bestow G‐type enriched lignin composition on the secondary cell walls of the vessel elements. Gene expression analyses suggested that PRN2 function is mediated by regulation of the expression of the lignin‐biosynthetic genes.
Place, publisher, year, edition, pages
New Phytologist Trust, 2020
Keywords
Arabidopsis, lignification, noncell-autonomy, PIRIN, xylem vessel element
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:umu:diva-165740 (URN)10.1111/nph.16271 (DOI)000495547800001 ()31625609 (PubMedID)2-s2.0-85074993523 (Scopus ID)
Funder
Swedish Research Council, 232-2011-1312Swedish Research Council Formas, 232-2011-1312Swedish Foundation for Strategic Research , RBP14-0011Vinnova, 201600504Knut and Alice Wallenberg Foundation, 20160341Bio4Energy
Available from: 2019-12-10 Created: 2019-12-10 Last updated: 2023-03-24Bibliographically approved
Zhang, B., Sztojka, B., Seyfferth, C., Escamez, S., Miskolczi, P., Chantreau, M., . . . Tuominen, H. (2020). The chromatin-modifying protein HUB2 is involved in the regulation of lignin composition in xylem vessels. Journal of Experimental Botany, 71(18), 5484-5494
Open this publication in new window or tab >>The chromatin-modifying protein HUB2 is involved in the regulation of lignin composition in xylem vessels
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2020 (English)In: Journal of Experimental Botany, ISSN 0022-0957, E-ISSN 1460-2431, Vol. 71, no 18, p. 5484-5494Article in journal (Refereed) Published
Abstract [en]

PIRIN2 (PRN2) was earlier reported to suppress syringyl (S)-type lignin accumulation of xylem vessels of Arabidopsis thaliana. In the present study, we report yeast two-hybrid results supporting the interaction of PRN2 with HISTONE MONOUBIQUITINATION2 (HUB2) in Arabidopsis. HUB2 has been previously implicated in several plant developmental processes, but not in lignification. Interaction between PRN2 and HUB2 was verified by β-galactosidase enzymatic and co-immunoprecipitation assays. HUB2 promoted the deposition of S-type lignin in the secondary cell walls of both stem and hypocotyl tissues, as analysed by pyrolysis-GC/MS. Chemical fingerprinting of individual xylem vessel cell walls by Raman and Fourier transform infrared microspectroscopy supported the function of HUB2 in lignin deposition. These results, together with a genetic analysis of the hub2 prn2 double mutant, support the antagonistic function of PRN2 and HUB2 in deposition of S-type lignin. Transcriptome analyses indicated the opposite regulation of the S-type lignin biosynthetic gene FERULATE-5-HYDROXYLASE1 by PRN2 and HUB2 as the underlying mechanism. PRN2 and HUB2 promoter activities co-localized in cells neighbouring the xylem vessel elements, suggesting that the S-type lignin-promoting function of HUB2 is antagonized by PRN2 for the benefit of the guaiacyl (G)-type lignin enrichment of the neighbouring xylem vessel elements.

Place, publisher, year, edition, pages
Oxford University Press, 2020
Keywords
Arabidopsis, cell wall chemistry, HUB2, lignin, PIRIN2, syringyl-type lignin, xylem vessels
National Category
Botany
Identifiers
urn:nbn:se:umu:diva-176306 (URN)10.1093/jxb/eraa264 (DOI)000577073500017 ()32479638 (PubMedID)2-s2.0-85096472238 (Scopus ID)
Available from: 2020-11-05 Created: 2020-11-05 Last updated: 2023-03-24Bibliographically approved
Lakehal, A., Dob, A., Rahneshan, Z., Novak, O., Escamez, S., Strnad, M., . . . Bellini, C. (2019). A Jasmonate-mediated molecular network provides cell-reprogramming decisions for organogenesis in Arabidopsis.
Open this publication in new window or tab >>A Jasmonate-mediated molecular network provides cell-reprogramming decisions for organogenesis in Arabidopsis
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2019 (English)Manuscript (preprint) (Other academic)
National Category
Developmental Biology
Identifiers
urn:nbn:se:umu:diva-164951 (URN)
Available from: 2019-11-05 Created: 2019-11-05 Last updated: 2019-11-06
Wessels, B., Seyfferth, C., Escamez, S., Vain, T., Antos, K., Vahala, J., . . . Tuominen, H. (2019). An AP2/ERF transcription factor ERF139 coordinates xylem cell expansion and secondary cell wall deposition. New Phytologist, 224(4), 1585-1599
Open this publication in new window or tab >>An AP2/ERF transcription factor ERF139 coordinates xylem cell expansion and secondary cell wall deposition
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2019 (English)In: New Phytologist, ISSN 0028-646X, E-ISSN 1469-8137, Vol. 224, no 4, p. 1585-1599Article in journal (Refereed) Published
Abstract [en]

Differentiation of xylem elements involves cell expansion, secondary cell wall (SCW) deposition and programmed cell death. Transitions between these phases require strict spatiotemporal control.

The function of Populus ERF139 (Potri.013G101100) in xylem differentiation was characterized in transgenic overexpression and dominant repressor lines of ERF139 in hybrid aspen (Populus tremula × tremuloides). Xylem properties, SCW chemistry and downstream targets were analyzed in both types of transgenic trees using microscopy techniques, Fourier transform‐infrared spectroscopy, pyrolysis‐GC/MS, wet chemistry methods and RNA sequencing.

Opposite phenotypes were observed in the secondary xylem vessel sizes and SCW chemistry in the two different types of transgenic trees, supporting the function of ERF139 in suppressing the radial expansion of vessel elements and stimulating accumulation of guaiacyl‐type lignin and possibly also xylan. Comparative transcriptomics identified genes related to SCW biosynthesis (LAC5, LBD15, MYB86) and salt and drought stress‐responsive genes (ANAC002, ABA1) as potential direct targets of ERF139.

The phenotypes of the transgenic trees and the stem expression profiles of ERF139potential target genes support the role of ERF139 as a transcriptional regulator of xylem cell expansion and SCW formation, possibly in response to osmotic changes of the cells.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2019
Keywords
cell expansion, ethylene response factor (ERF), hybrid aspen, lignin, Populus, secondary growth, xylem development
National Category
Botany
Identifiers
urn:nbn:se:umu:diva-161696 (URN)10.1111/nph.15960 (DOI)000474107100001 ()31125440 (PubMedID)2-s2.0-85068612698 (Scopus ID)
Projects
Bio4Energy
Funder
Swedish Research Council Formas, 213-2011-1148Swedish Research Council Formas, 239-2011-1915The Kempe Foundations, SMK-1649The Kempe Foundations, SMK-1533Swedish Foundation for Strategic Research , RBP14-0011Knut and Alice Wallenberg Foundation, 2016-0341Bio4Energy
Available from: 2019-08-06 Created: 2019-08-06 Last updated: 2020-06-17Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0002-4949-3702

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