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Publications (10 of 95) Show all publications
Bellini, C. (2024). A synthetic auxin for cloning mature trees [Letter to the editor]. Nature Biotechnology
Open this publication in new window or tab >>A synthetic auxin for cloning mature trees
2024 (English)In: Nature Biotechnology, ISSN 1087-0156, E-ISSN 1546-1696Article in journal, Letter (Other academic) Epub ahead of print
Place, publisher, year, edition, pages
Springer Nature, 2024
National Category
Botany Plant Biotechnology
Identifiers
urn:nbn:se:umu:diva-220466 (URN)10.1038/s41587-024-02132-3 (DOI)38267758 (PubMedID)2-s2.0-85183434592 (Scopus ID)
Available from: 2024-02-05 Created: 2024-02-05 Last updated: 2024-02-05Bibliographically approved
Zeng, Y., Verstraeten, I., Trinh, H. K., Lardon, R., Schotte, S., Olatunji, D., . . . Geelen, D. (2023). Chemical induction of hypocotyl rooting reveals extensive conservation of auxin signalling controlling lateral and adventitious root formation. New Phytologist, 240(5), 1883-1899
Open this publication in new window or tab >>Chemical induction of hypocotyl rooting reveals extensive conservation of auxin signalling controlling lateral and adventitious root formation
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2023 (English)In: New Phytologist, ISSN 0028-646X, E-ISSN 1469-8137, Vol. 240, no 5, p. 1883-1899Article in journal (Refereed) Published
Abstract [en]

Upon exposure to light, etiolated Arabidopsis seedlings form adventitious roots (AR) along the hypocotyl. While processes underlying lateral root formation are studied intensively, comparatively little is known about the molecular processes involved in the initiation of hypocotyl AR. AR and LR formation were studied using a small molecule named Hypocotyl Specific Adventitious Root INducer (HYSPARIN) that strongly induces AR but not LR formation. HYSPARIN does not trigger rapid DR5-reporter activation, DII-Venus degradation or Ca2+ signalling. Transcriptome analysis, auxin signalling reporter lines and mutants show that HYSPARIN AR induction involves nuclear TIR1/AFB and plasma membrane TMK auxin signalling, as well as multiple downstream LR development genes (SHY2/IAA3, PUCHI, MAKR4 and GATA23). Comparison of the AR and LR induction transcriptome identified SAURs, AGC kinases and OFP transcription factors as specifically upregulated by HYSPARIN. Members of the SAUR19 subfamily, OFP4 and AGC2 suppress HYS-induced AR formation. While SAUR19 and OFP subfamily members also mildly modulate LR formation, AGC2 regulates only AR induction. Analysis of HYSPARIN-induced AR formation uncovers an evolutionary conservation of auxin signalling controlling LR and AR induction in Arabidopsis seedlings and identifies SAUR19, OFP4 and AGC2 kinase as novel regulators of AR formation.

Place, publisher, year, edition, pages
New Phytologist Foundation, 2023
Keywords
adventitious root, auxin signalling, root branching, root development, synthetic auxin
National Category
Botany
Identifiers
urn:nbn:se:umu:diva-215362 (URN)10.1111/nph.19292 (DOI)37787103 (PubMedID)2-s2.0-85173432491 (Scopus ID)
Available from: 2023-10-31 Created: 2023-10-31 Last updated: 2023-12-19Bibliographically approved
Kidwai, M., Mishra, P. & Bellini, C. (2023). Species-specific transcriptional reprogramming during adventitious root initiation. Trends in Plant Science, 28(2), 128-130
Open this publication in new window or tab >>Species-specific transcriptional reprogramming during adventitious root initiation
2023 (English)In: Trends in Plant Science, ISSN 1360-1385, E-ISSN 1878-4372, Vol. 28, no 2, p. 128-130Article in journal (Refereed) Published
Abstract [en]

Adventitious roots or shoot-borne roots transdifferentiate from cells close to vascular tissues after cell reprogramming, which is associated with increased transcriptional activity. Recently, Garg et al. provided a genome-wide landscape of transcriptional signatures during the early stages of adventitious root initiation in rice and showed that conserved transcription factors acquire species-specific function.

Place, publisher, year, edition, pages
Elsevier, 2023
Keywords
adventitious root, dicotyledons, epigenetic regulation, monocotyledons, transcription factors
National Category
Botany
Identifiers
urn:nbn:se:umu:diva-201342 (URN)10.1016/j.tplants.2022.11.003 (DOI)000961261300001 ()36396568 (PubMedID)2-s2.0-85141960884 (Scopus ID)
Available from: 2022-12-14 Created: 2022-12-14 Last updated: 2023-05-02Bibliographically approved
Aubry, E., Hoffmann, B., Vilaine, F., Gilard, F., Klemens, P. A., Guérard, F., . . . Le Hir, R. (2022). A vacuolar hexose transport is required for xylem development in the inflorescence stem. Plant Physiology, 188(2), 1229-1247
Open this publication in new window or tab >>A vacuolar hexose transport is required for xylem development in the inflorescence stem
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2022 (English)In: Plant Physiology, ISSN 0032-0889, E-ISSN 1532-2548, Vol. 188, no 2, p. 1229-1247Article in journal (Refereed) Published
Abstract [en]

In Angiosperms, the development of the vascular system is controlled by a complex network of transcription factors. However, how nutrient availability in the vascular cells affects their development remains to be addressed. At the cellular level, cytosolic sugar availability is regulated mainly by sugar exchanges at the tonoplast through active and/or facilitated transport. In Arabidopsis (Arabidopsis thaliana), among the genes encoding tonoplastic transporters, SUGAR WILL EVENTUALLY BE EXPORTED TRANSPORTER 16 (SWEET16) and SWEET17 expression has been previously detected in the vascular system. Here, using a reverse genetics approach, we propose that sugar exchanges at the tonoplast, regulated by SWEET16, are important for xylem cell division as revealed in particular by the decreased number of xylem cells in the swt16 mutant and the accumulation of SWEET16 at the procambium-xylem boundary. In addition, we demonstrate that transport of hexoses mediated by SWEET16 and/or SWEET17 is required to sustain the formation of the xylem secondary cell wall. This result is in line with a defect in the xylem cell wall composition as measured by Fourier-transformed infrared spectroscopy in the swt16swt17 double mutant and by upregulation of several genes involved in secondary cell wall synthesis. Our work therefore supports a model in which xylem development partially depends on the exchange of hexoses at the tonoplast of xylem-forming cells.

Place, publisher, year, edition, pages
Oxford University Press, 2022
National Category
Botany
Identifiers
urn:nbn:se:umu:diva-192644 (URN)10.1093/plphys/kiab551 (DOI)000761476800047 ()34865141 (PubMedID)2-s2.0-85124435567 (Scopus ID)
Available from: 2022-02-22 Created: 2022-02-22 Last updated: 2023-09-05Bibliographically approved
Cardoso, H., Peixe, A., Bellini, C., Porfírio, S. & Druege, U. (2022). Editorial: Advances on the Biological Mechanisms Involved in Adventitious Root Formation: From Signaling to Morphogenesis. Frontiers in Plant Science, 13, Article ID 867651.
Open this publication in new window or tab >>Editorial: Advances on the Biological Mechanisms Involved in Adventitious Root Formation: From Signaling to Morphogenesis
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2022 (English)In: Frontiers in Plant Science, E-ISSN 1664-462X, Vol. 13, article id 867651Article in journal, Editorial material (Other academic) Published
Place, publisher, year, edition, pages
Frontiers Media S.A., 2022
Keywords
cell reprogramming, hormone crosstalk, reactive oxygen species (ROS), rooting recalcitrance, transcription factors, vegetative propagation
National Category
Botany
Identifiers
urn:nbn:se:umu:diva-193685 (URN)10.3389/fpls.2022.867651 (DOI)000776236700001 ()35295629 (PubMedID)2-s2.0-85127069675 (Scopus ID)
Available from: 2022-05-03 Created: 2022-05-03 Last updated: 2024-01-17Bibliographically approved
Ranjan, A., Perrone, I., Alallaq, S., Singh, R., Rigal, A., Brunoni, F., . . . Bellini, C. (2022). Molecular basis of differential adventitious rooting competence in poplar genotypes. Journal of Experimental Botany, 73(12), 4046-4064
Open this publication in new window or tab >>Molecular basis of differential adventitious rooting competence in poplar genotypes
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2022 (English)In: Journal of Experimental Botany, ISSN 0022-0957, E-ISSN 1460-2431, Vol. 73, no 12, p. 4046-4064Article in journal (Refereed) Published
Abstract [en]

Recalcitrant adventitious root (AR) development is a major hurdle in propagating commercially important woody plants. Although significant progress has been made to identify genes involved in subsequent steps of AR development, the molecular basis of differences in apparent recalcitrance to form AR between easy-to-root and difficult-to-root genotypes remains unknown. To address this, we generated cambium tissue-specific transcriptomic data from stem cuttings of hybrid aspen, T89 (difficult-to-root) and hybrid poplar OP42 (easy-to-root), and used transgenic approaches to verify the role of several transcription factors in the control of adventitious rooting. Increased peroxidase activity was positively correlated with better rooting. We found differentially expressed genes encoding reactive oxygen species scavenging proteins to be enriched in OP42 compared with T89. A greater number of differentially expressed transcription factors in cambium cells of OP42 compared with T89 was revealed by a more intense transcriptional reprograming in the former. PtMYC2, a potential negative regulator, was less expressed in OP42 compared with T89. Using transgenic approaches, we demonstrated that PttARF17.1 and PttMYC2.1 negatively regulate adventitious rooting. Our results provide insights into the molecular basis of genotypic differences in AR and implicate differential expression of the master regulator MYC2 as a critical player in this process.

Place, publisher, year, edition, pages
Oxford University Press, 2022
Keywords
Adventitious roots, cambium, hybrid aspen, hybrid poplar, Populus spp, stem cuttings
National Category
Botany
Identifiers
urn:nbn:se:umu:diva-198225 (URN)10.1093/jxb/erac126 (DOI)000813477900001 ()35325111 (PubMedID)2-s2.0-85133797842 (Scopus ID)
Funder
Knut and Alice Wallenberg FoundationVinnovaSwedish Research CouncilSwedish Research Council FormasThe Kempe FoundationsCarl Tryggers foundation
Available from: 2022-07-21 Created: 2022-07-21 Last updated: 2024-07-02Bibliographically approved
Chardon, F., De Marco, F., Marmagne, A., Le Hir, R., Vilaine, F., Bellini, C. & Dinant, S. (2022). Natural variation in the long-distance transport of nutrients and photoassimilates in response to N availability. Journal of plant physiology (Print), 273, Article ID 153707.
Open this publication in new window or tab >>Natural variation in the long-distance transport of nutrients and photoassimilates in response to N availability
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2022 (English)In: Journal of plant physiology (Print), ISSN 0176-1617, E-ISSN 1618-1328, Vol. 273, article id 153707Article in journal (Refereed) Published
Abstract [en]

Phloem and xylem tissues are necessary for the allocation of nutrients and photoassimilates. However, how the long-distance transport of carbon (C) and nitrogen (N) is coordinated with the central metabolism is largely unknown. To better understand how the genetic and environmental factors influence C and N transport, we analysed the metabolite profiles of phloem exudates and xylem saps of five Arabidopsis thaliana accessions grown in low or non-limiting N supply. We observed that xylem saps were composed of 46 or 56% carbohydrates, 27 or 45% amino acids, and 5 or 13% organic acids in low or non-limiting N supply, respectively. In contrast, phloem exudates were composed of 76 or 86% carbohydrates, 7 or 18% amino acids, and 5 or 6% organic acids. Variation in N supply impacted amino acid, organic acid and sugar contents. When comparing low N and non-limiting N, the most striking differences were variations of glutamine, aspartate, and succinate abundance in the xylem saps and citrate and fumarate abundance in phloem exudates. In addition, we observed a substantial variation of metabolite content between genotypes, particularly under high N. The content of several organic acids, such as malate, citrate, fumarate, and succinate was affected by the genotype alone or by the interaction between genotype and N supply. This study confirmed that the response of the transport of nutrients in the phloem and the xylem to N availability is associated with the regulation of the central metabolism and could be an adaptive trait.

Place, publisher, year, edition, pages
Elsevier, 2022
Keywords
Allocation, Pipecolate, Raffinose, Succinate, Sucrose, Transport
National Category
Botany
Identifiers
urn:nbn:se:umu:diva-194887 (URN)10.1016/j.jplph.2022.153707 (DOI)2-s2.0-85129487323 (Scopus ID)
Available from: 2022-06-07 Created: 2022-06-07 Last updated: 2022-06-07Bibliographically approved
Bannoud, F. & Bellini, C. (2021). Adventitious Rooting in Populus Species: Update and Perspectives. Frontiers in Plant Science, 12, Article ID 668837.
Open this publication in new window or tab >>Adventitious Rooting in Populus Species: Update and Perspectives
2021 (English)In: Frontiers in Plant Science, E-ISSN 1664-462X, Vol. 12, article id 668837Article, review/survey (Refereed) Published
Abstract [en]

Populus spp. are among the most economically important species worldwide. These trees are used not only for wood and fiber production, but also in the rehabilitation of degraded lands. Since they are clonally propagated, the ability of stem cuttings to form adventitious roots is a critical point for plant establishment and survival in the field, and consequently for the forest industry. Adventitious rooting in different Populus clones has been an agronomic trait targeted in breeding programs for many years, and many factors have been identified that affect this quantitative trait. A huge variation in the rooting capacity has been observed among the species in the Populus genus, and the responses to some of the factors affecting this trait have been shown to be genotype-dependent. This review analyses similarities and differences between results obtained from studies examining the role of internal and external factors affecting rooting of Populus species cuttings. Since rooting is the most important requirement for stand establishment in clonally propagated species, understanding the physiological and genetic mechanisms that promote this trait is essential for successful commercial deployment.

Place, publisher, year, edition, pages
Frontiers Media S.A., 2021
Keywords
adventitious rooting, endogenous factors, environmental factors, Populus, vegetative propagation
National Category
Botany
Identifiers
urn:nbn:se:umu:diva-184458 (URN)10.3389/fpls.2021.668837 (DOI)000657466100001 ()2-s2.0-85107287124 (Scopus ID)
Available from: 2021-06-14 Created: 2021-06-14 Last updated: 2024-01-17Bibliographically approved
Dob, A., Lakehal, A., Novak, O. & Bellini, C. (2021). Jasmonate inhibits adventitious root initiation through repression of CKX1 and activation of RAP2.6L transcription factor in Arabidopsis. Journal of Experimental Botany, 72(20), 7107-7118
Open this publication in new window or tab >>Jasmonate inhibits adventitious root initiation through repression of CKX1 and activation of RAP2.6L transcription factor in Arabidopsis
2021 (English)In: Journal of Experimental Botany, ISSN 0022-0957, E-ISSN 1460-2431, Vol. 72, no 20, p. 7107-7118Article in journal (Refereed) Published
Abstract [en]

Adventitious rooting is a de novo organogenesis process that enables plants to propagate clonally and cope with environmental stresses. Adventitious root initiation (ARI) is controlled by interconnected transcriptional and hormonal networks, but there is little knowledge of the genetic and molecular programs orchestrating these networks. Thus, we have applied genome-wide transcriptome profiling to elucidate the transcriptional reprogramming events preceding ARI. These reprogramming events are associated with the down-regulation of cytokinin (CK) signaling and response genes, which could be triggers for ARI. Interestingly, we found that CK free base (iP, tZ, cZ, and DHZ) content declined during ARI, due to down-regulation of de novo CK biosynthesis and up-regulation of CK inactivation pathways. We also found that MYC2-dependent jasmonate (JA) signaling inhibits ARI by down-regulating the expression of the CYTOKININ OXIDASE/DEHYDROGENASE1 (CKX1) gene. We also demonstrated that JA and CK synergistically activate expression of the transcription factor RELATED to APETALA2.6 LIKE (RAP2.6L), and constitutive expression of this transcription factor strongly inhibits ARI. Collectively, our findings reveal that previously unknown genetic interactions between JA and CK play key roles in ARI.

Place, publisher, year, edition, pages
Oxford University Press, 2021
Keywords
Adventitious roots, Arabidopsis, CKX1, cytokinins, jasmonate, light, MYC2, RAP2.6L, vegetative propagation
National Category
Botany
Identifiers
urn:nbn:se:umu:diva-189938 (URN)10.1093/jxb/erab358 (DOI)000715558300024 ()34329421 (PubMedID)2-s2.0-85119363747 (Scopus ID)
Funder
Swedish Research Council, 2020–06430VinnovaKnut and Alice Wallenberg FoundationCarl Tryggers foundation The Kempe Foundations
Available from: 2021-11-29 Created: 2021-11-29 Last updated: 2021-11-29Bibliographically approved
Brunoni, F., Collani, S., Casanova-Saez, R., Simura, J., Karady, M., Schmid, M., . . . Bellini, C. (2020). Conifers exhibit a characteristic inactivation of auxin to maintain tissue homeostasis. New Phytologist, 226(6), 1753-1765
Open this publication in new window or tab >>Conifers exhibit a characteristic inactivation of auxin to maintain tissue homeostasis
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2020 (English)In: New Phytologist, ISSN 0028-646X, E-ISSN 1469-8137, Vol. 226, no 6, p. 1753-1765Article in journal (Refereed) Published
Abstract [en]

Dynamic regulation of the concentration of the natural auxin (IAA) is essential to coordinate most of the physiological and developmental processes and responses to environmental changes. Oxidation of IAA is a major pathway to control auxin concentrations in angiosperms and, along with IAA conjugation, to respond to perturbation of IAA homeostasis. However, these regulatory mechanisms remain poorly investigated in conifers. To reduce this knowledge gap, we investigated the different contributions of the IAA inactivation pathways in conifers. MS-based quantification of IAA metabolites under steady-state conditions and after perturbation was investigated to evaluate IAA homeostasis in conifers. Putative Picea abies GH3 genes (PaGH3) were identified based on a comprehensive phylogenetic analysis including angiosperms and basal land plants. Auxin-inducible PaGH3 genes were identified by expression analysis and their IAA-conjugating activity was explored. Compared to Arabidopsis, oxidative and conjugative pathways differentially contribute to reduce IAA concentrations in conifers. We demonstrated that the oxidation pathway plays a marginal role in controlling IAA homeostasis in spruce. By contrast, an excess of IAA rapidly activates GH3-mediated irreversible conjugation pathways. Taken together, these data indicate that a diversification of IAA inactivation mechanisms evolved specifically in conifers.

Place, publisher, year, edition, pages
John Wiley & Sons, 2020
Keywords
auxin conjugates, auxin homeostasis, conifers, GH3 genes, indole-3-acetic acid (IAA), Picea abies
National Category
Developmental Biology
Identifiers
urn:nbn:se:umu:diva-171948 (URN)10.1111/nph.16463 (DOI)000533328500021 ()32004385 (PubMedID)2-s2.0-85080875275 (Scopus ID)
Available from: 2020-06-18 Created: 2020-06-18 Last updated: 2023-03-24Bibliographically approved
Projects
Role of WBC and nodulin transmembrane proteins in phloem differentiation and functionning [2008-511_Formas]; Umeå UniversityUnravelling molecular mechanisms regulating adventitious root formation in Arabidospsis thaliana [2010-04359_VR]; Umeå UniversityControl of adventitious root initiation in Arabidopsis thaliana: toward a predictive model [2013-04083_VR]; Umeå UniversityImproving Norway spruce root system architecture for sustainable forestry [2015-923_Formas]; Umeå UniversityControl of adventitious root initiation in Arabidopsis thaliana: deciphering the increasing complexity of molecular cross-talks [2017-03841_VR]; Umeå University
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0003-2985-6649

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