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Laumonnerie, Christophe
Publications (3 of 3) Show all publications
Wurmser, M., Muppavarapu, M., Tait, C. M., Laumonnerie, C., Gonzalez-Castrillon, L. M. & Wilson, S. I. (2021). Robo2 Receptor Gates the Anatomical Divergence of Neurons Derived From a Common Precursor Origin. Frontiers in Cell and Developmental Biology, 9, Article ID 668175.
Open this publication in new window or tab >>Robo2 Receptor Gates the Anatomical Divergence of Neurons Derived From a Common Precursor Origin
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2021 (English)In: Frontiers in Cell and Developmental Biology, E-ISSN 2296-634X, Vol. 9, article id 668175Article in journal (Refereed) Published
Abstract [en]

Sensory information relayed to the brain is dependent on complex, yet precise spatial organization of neurons. This anatomical complexity is generated during development from a surprisingly small number of neural stem cell domains. This raises the question of how neurons derived from a common precursor domain respond uniquely to their environment to elaborate correct spatial organization and connectivity. We addressed this question by exploiting genetically labeled mouse embryonic dorsal interneuron 1 (dI1) neurons that are derived from a common precursor domain and give rise to spinal projection neurons with distinct organization of cell bodies with axons projecting either commissurally (dI1c) or ipsilaterally (dI1i). In this study, we examined how the guidance receptor, Robo2, which is a canonical Robo receptor, influenced dI1 guidance during embryonic development. Robo2 was enriched in embryonic dI1i neurons, and loss of Robo2 resulted in misguidance of dI1i axons, whereas dI1c axons remained unperturbed within the mantle zone and ventral commissure. Further, Robo2 profoundly influenced dI1 cell body migration, a feature that was partly dependent on Slit2 signaling. These data suggest that dI1 neurons are dependent on Robo2 for their organization. This work integrated with the field support of a model whereby canonical Robo2 vs. non-canonical Robo3 receptor expression facilitates projection neurons derived from a common precursor domain to read out the tissue environment uniquely giving rise to correct anatomical organization.

Place, publisher, year, edition, pages
Frontiers Media S.A., 2021
Keywords
migration, axon guidance, robo receptors, neural development, commissural neuron, ipsilateral neuron, neural organization, sensory neuron
National Category
Neurosciences
Identifiers
urn:nbn:se:umu:diva-187303 (URN)10.3389/fcell.2021.668175 (DOI)000670393700001 ()34249921 (PubMedID)2-s2.0-85115904413 (Scopus ID)
Funder
Swedish Research Council, 2015-05289The Kempe FoundationsCarl Tryggers foundation
Available from: 2021-09-08 Created: 2021-09-08 Last updated: 2023-11-20Bibliographically approved
Laumonnerie, C., Tong, Y. G., Alstermark, H. & Wilson, S. I. (2015). Commissural axonal corridors instruct neuronal migration in the mouse spinal cord. Nature Communications, 6, Article ID 7028.
Open this publication in new window or tab >>Commissural axonal corridors instruct neuronal migration in the mouse spinal cord
2015 (English)In: Nature Communications, E-ISSN 2041-1723, Vol. 6, article id 7028Article in journal (Refereed) Published
Abstract [en]

Unravelling how neurons are guided during vertebrate embryonic development has wide implications for understanding the assembly of the nervous system. During embryogenesis, migration of neuronal cell bodies and axons occurs simultaneously, but to what degree they influence each other's development remains obscure. We show here that within the mouse embryonic spinal cord, commissural axons bisect, delimit or preconfigure ventral interneuron cell body position. Furthermore, genetic disruption of commissural axons results in abnormal ventral interneuron cell body positioning. These data suggest that commissural axonal fascicles instruct cell body position by acting either as border landmarks (axon-restricted migration), which to our knowledge has not been previously addressed, or acting as cellular guides. This study in the developing spinal cord highlights an important function for the interaction of cell bodies and axons, and provides a conceptual proof of principle that is likely to have overarching implications for the development of neuronal architecture.

National Category
Neurosciences
Identifiers
urn:nbn:se:umu:diva-106511 (URN)10.1038/ncomms8028 (DOI)000355530100002 ()25960414 (PubMedID)2-s2.0-84929190837 (Scopus ID)
Available from: 2015-07-15 Created: 2015-07-14 Last updated: 2023-03-28Bibliographically approved
Laumonnerie, C., Da Silva, R. V., Kania, A. & Wilson, S. (2014). Netrin 1 and Dcc signalling are required for confinement of central axons within the central nervous system. Development, 141(3), 594-603
Open this publication in new window or tab >>Netrin 1 and Dcc signalling are required for confinement of central axons within the central nervous system
2014 (English)In: Development, ISSN 0950-1991, E-ISSN 1477-9129, Vol. 141, no 3, p. 594-603Article in journal (Refereed) Published
Abstract [en]

The establishment of anatomically stereotyped axonal projections is fundamental to neuronal function. While most neurons project their axons within the central nervous system (CNS), only axons of centrally born motoneurons and peripherally born sensory neurons link the CNS and peripheral nervous system (PNS) together by navigating through specialized CNS/PNS transition zones. Such selective restriction is of importance because inappropriate CNS axonal exit could lead to loss of correct connectivity and also to gain of erroneous functions. However, to date, surprisingly little is known about the molecular-genetic mechanisms that regulate how central axons are confined within the CNS during development. Here, we show that netrin 1/Dcc/Unc5 chemotropism contributes to axonal confinement within the CNS. In both Ntn1 and Dcc mutant mouse embryos, some spinal interneuronal axons exit the CNS by traversing the CNS/PNS transition zones normally reserved for motor and sensory axons. We provide evidence that netrin 1 signalling preserves CNS/PNS axonal integrity in three ways: (1) netrin 1/Dcc ventral attraction diverts axons away from potential exit points; (2) a Dcc/Unc5c-dependent netrin 1 chemoinhibitory barrier in the dorsolateral spinal cord prevents interneurons from being close to the dorsal CNS/PNS transition zone; and (3) a netrin 1/Dcc-dependent, Unc5c-independent mechanism that actively prevents exit from the CNS. Together, these findings provide insights into the molecular mechanisms that maintain CNS/PNS integrity and, to the best of our knowledge, present the first evidence that chemotropic signalling regulates interneuronal CNS axonal confinement in vertebrates.

Keywords
Netrin 1, Dcc, Spinal cord, CNS exit, Unc5, Axonal confinement, Mouse
National Category
General Practice
Identifiers
urn:nbn:se:umu:diva-86827 (URN)10.1242/dev.099606 (DOI)000330573500011 ()2-s2.0-84892696823 (Scopus ID)
Available from: 2014-03-17 Created: 2014-03-11 Last updated: 2023-03-24Bibliographically approved
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