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Hedberg, Maria E.
Alternative names
Publications (10 of 20) Show all publications
Johansson, A. & Hedberg, M. E. (2024). Fermented rye bran as biocide replacement in paper production. In: Marzena Smol (Ed.), 4th international conference strategies toward green deal implementation water, raw materials & energy: Abstract book. Paper presented at 4th International Conference Strategies toward Green Deal Implementation Water, Raw Materials & Energy, Online conference, 14-15 December, 2023. (pp. 78-78). Cracow: Publishing House, Mineral and Energy Economy Research Institute, Polish Academy of Sciences
Open this publication in new window or tab >>Fermented rye bran as biocide replacement in paper production
2024 (English)In: 4th international conference strategies toward green deal implementation water, raw materials & energy: Abstract book / [ed] Marzena Smol, Cracow: Publishing House, Mineral and Energy Economy Research Institute, Polish Academy of Sciences , 2024, p. 78-78Conference paper, Oral presentation with published abstract (Refereed)
Abstract [en]

Microbial contamination causes environmental and costly problems in paper production. Today, chemical-based biocides are used to control these problems. We have developed a biocide substitute that consists of fermented rest products from the cereal industry. Rye bran is a suitable nutrient for growth of a selected strain of Lactiplantibacillus plantarum. Previous studies have shown that fermented rye bran contains bioactive metabolites that powerfully limit virtually all bacterial growth. The cereals also contain fibres with potential to be a supplement in the raw material (pulp) of paper production. Our intention with this study was to investigate the potential of fermented rye bran as an antibacterial raw material in paper production. We will specifically determine the antibacterial effect of fermented rye bran on bacterial strains isolated from pulp and process water at a paper mill. In addition, to analyse the effect of the single metabolites discovered in the fermented rye bran. The results showed that fermented rye bran inhibited growth of the majority of the bacterial strains isolated from the paper mill. The pure metabolites showed also antibacterial properties, but less potent in comparison to the crude fermented rye bran product. The Lactobacillus strain used in the present study was resistant to all the tested metabolites. In conclusion, fermented rye bran shows properties indicating potential to be used as a bioactive raw material, limiting the need to add antimicrobial chemicals in paper production

Place, publisher, year, edition, pages
Cracow: Publishing House, Mineral and Energy Economy Research Institute, Polish Academy of Sciences, 2024
Keywords
Paper production, Lactoplantibacillus plantarum, fermentation, rye bran, antimicrobials
National Category
Biological Sciences Food Science Environmental Sciences
Research subject
environmental change
Identifiers
urn:nbn:se:umu:diva-221714 (URN)978-83-67606-27-1 (ISBN)
Conference
4th International Conference Strategies toward Green Deal Implementation Water, Raw Materials & Energy, Online conference, 14-15 December, 2023.
Available from: 2024-03-03 Created: 2024-03-03 Last updated: 2024-03-04Bibliographically approved
Johansson, A. & Hedberg, M. E. (2024). Industrial rest materials as biocide replacement in paper production. In: Research workshop on bacillus in clinical and agricultural environments: . Paper presented at Research Workshop on Bacillus in Clinical and Agricultural Environments, Umeå, Sweden, February 29 February - March 1, 2024 (pp. 15-15). German Multidisciplinary Publishing Center
Open this publication in new window or tab >>Industrial rest materials as biocide replacement in paper production
2024 (English)In: Research workshop on bacillus in clinical and agricultural environments, German Multidisciplinary Publishing Center , 2024, p. 15-15Conference paper, Oral presentation with published abstract (Refereed)
Abstract [en]

Microbial contamination causes environmental and costly problems in paper production (gas formation in stored pulp, and biofilm/slime) and waste water systems (biofilm formation causing corrosion of the pipes). Today, chemical-based biocides are used to control these problems. We have developed a biocide substitute consisting of industrial rest products; rye bran or spruce bark. Rye bran is a suitable nutrient for growth of a selected strain of Lactiplantibacillus plantarum. Previous studies have shown that fermented rye bran, as well as spruce bark extracts, contains bioactive metabolites with the power to limit virtually all bacterial growth. These products also contain fibres with potential to be a supplement as raw material in the pulp.

Our intention with this study was to investigate the potential of these rest materials as an antibacterial raw material in paper production. We will specifically determine the antibacterial effect on strains isolated from pulp, process water from the paper mill, and waste water pipes. In addition, likewise analyse the antimicrobial activity of single metabolites discovered in the crude extracts. The results so far, show that the products efficiently inhibit growth of the bacterial strains isolated from waste water and the paper mill. The pure metabolites showed also antibacterial properties, but were less potent as compared to the crude products. The Lactiplantiobacillus strain, used to ferment the rye bran in the present study, was resistant to all the tested products.

In conclusion, fermented rye bran and spruce bark extracts show properties indicating a potential to be used as a bioactive raw material, limiting the need of adding synthetic biocides in paper production and waste water systems.

Place, publisher, year, edition, pages
German Multidisciplinary Publishing Center, 2024
Series
GMPC thesis & opinions platform, E-ISSN 2747-5735 ; 2024:4(1)
Keywords
Paper production, Lactiplantibacillus plantarum, fermentation, rye bran, spruce bark extracts, biocide replacement
National Category
Agricultural Biotechnology
Research subject
biological chemistry
Identifiers
urn:nbn:se:umu:diva-221715 (URN)10.51585/gtop.2024.1.0036 (DOI)
Conference
Research Workshop on Bacillus in Clinical and Agricultural Environments, Umeå, Sweden, February 29 February - March 1, 2024
Available from: 2024-03-03 Created: 2024-03-03 Last updated: 2024-03-26Bibliographically approved
Koistinen, V. M., Hedberg, M., Shi, L., Johansson, A., Savolainen, O., Lehtonen, M., . . . Landberg, R. (2022). Metabolite pattern derived from Lactiplantibacillus plantarum: fermented rye foods and in vitro gut fermentation synergistically inhibits bacterial growth. Molecular Nutrition & Food Research, 66(21), Article ID 2101096.
Open this publication in new window or tab >>Metabolite pattern derived from Lactiplantibacillus plantarum: fermented rye foods and in vitro gut fermentation synergistically inhibits bacterial growth
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2022 (English)In: Molecular Nutrition & Food Research, ISSN 1613-4125, E-ISSN 1613-4133, Vol. 66, no 21, article id 2101096Article in journal (Refereed) Published
Abstract [en]

Scope: Fermentation improves many food characteristics using microbes, such as lactic acid bacteria (LAB). Recent studies suggest fermentation may also enhance the health properties, but mechanistic evidence is lacking. We aimed to identify a metabolite pattern reproducibly produced during sourdough and in vitro colonic fermentation of various whole-grain rye products and how it affects the growth of bacterial species of potential importance to health and disease.

Methods and results: We used Lactiplantibacillus plantarum DSMZ 13890 strain, previously shown to favour rye as its substrate. Using LC-MS metabolomics, we found seven microbial metabolites commonly produced during the fermentations, including dihydroferulic acid, dihydrocaffeic acid, and five amino acid metabolites, and stronger inhibition was achieved when exposing the bacteria to a mixture of the metabolites in vitro compared to individual compound exposures.

Conclusion: Our study suggests that metabolites produced by LAB may synergistically modulate the local microbial ecology, such as in the gut. This could provide new hypotheses on how fermented foods influence human health via diet–microbiota interactions.

Place, publisher, year, edition, pages
John Wiley & Sons, 2022
Keywords
Probiotics, Nutrition, Rye bran, fermentation, lactobacilli, metabolites, microbiota, rye
National Category
Nutrition and Dietetics Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Research subject
Nutrition
Identifiers
urn:nbn:se:umu:diva-198585 (URN)10.1002/mnfr.202101096 (DOI)000842344400001 ()35960594 (PubMedID)2-s2.0-85136471943 (Scopus ID)
Funder
Swedish Energy Agency, P244-220-1VinnovaAcademy of Finland, 321716Academy of Finland, 334814Swedish Research CouncilEU, Horizon 2020, 754412
Available from: 2022-08-12 Created: 2022-08-12 Last updated: 2022-12-30Bibliographically approved
Moore, E. R. .., Salvà‐Serra, F., Jaén‐Luchoro, D., Hammarström, M.-L., Hammarström, S. & Hedberg, M. E. (2021). Lachnoanaerobaculum. In: Bergey's Manual of Systematics of Archaea and Bacteria (BMSAB): . John Wiley & Sons
Open this publication in new window or tab >>Lachnoanaerobaculum
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2021 (English)In: Bergey's Manual of Systematics of Archaea and Bacteria (BMSAB), John Wiley & Sons, 2021Chapter in book (Refereed)
Abstract [en]

The genus Lachnoanaerobaculum comprises obligately anaerobic, Gram-stain-positive chemoorganotrophic, saccharolytic, and nonproteolytic bacilli. Cells are spore forming, rod shaped, and filamentous, 5 to greater than 20 μm in length, some cells with curving and swelling. All species of the genus grow with glucose as the sole carbon source. All species produce H2S, NH3, butyric acid, acetic acid, and lactic acid as metabolic end products. The predominant cellular fatty acids are C14:0, C16:0, and C18:1 ω7c DMA. The G + C contents of genomic DNA of the species are 35.0–37.8 mol%. Phylogenetic relationships of the species of Lachnoanaerobaculum, based on comparative 16S rRNA gene sequence analyses, indicate that they cluster within the phylum Firmicutes, within the family Lachnospiraceae, and exhibit a clear delineation to the other genera of the family, with a relatively close relationship to Johnsonella species. The first described strain of Lachnoanaerobaculum umeaense, the type species of the genus, was isolated from the jejunal mucosa of a child with coeliac disease. Strains of the species of Lachnoanaerobaculum are typically found in intestinal microbiota and oral microbiota of the human microbiome, isolated from the human gut, saliva, blood, amniotic fluid, and, predominantly, from the oral cavity.

Place, publisher, year, edition, pages
John Wiley & Sons, 2021
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:umu:diva-192279 (URN)10.1002/9781118960608.gbm02007 (DOI)9781118960608 (ISBN)
Available from: 2022-02-07 Created: 2022-02-07 Last updated: 2022-02-07Bibliographically approved
Pietz, G., De, R., Hedberg, M., Sjöberg, V., Sandström, O., Hernell, O., . . . Hammarström, M.-L. (2017). Immunopathology of childhood celiac disease: Key role of intestinal epithelial cells. PLOS ONE, 12(9), Article ID e0185025.
Open this publication in new window or tab >>Immunopathology of childhood celiac disease: Key role of intestinal epithelial cells
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2017 (English)In: PLOS ONE, E-ISSN 1932-6203, Vol. 12, no 9, article id e0185025Article in journal (Refereed) Published
Abstract [en]

BACKGROUND & AIMS: Celiac disease is a chronic inflammatory disease of the small intestine mucosa due to permanent intolerance to dietary gluten. The aim was to elucidate the role of small intestinal epithelial cells in the immunopathology of celiac disease in particular the influence of celiac disease-associated bacteria.

METHODS: Duodenal biopsies were collected from children with active celiac disease, treated celiac disease, and clinical controls. Intestinal epithelial cells were purified and analyzed for gene expression changes at the mRNA and protein levels. Two in vitro models for human intestinal epithelium, small intestinal enteroids and polarized tight monolayers, were utilized to assess how interferon-γ, interleukin-17A, celiac disease-associated bacteria and gluten influence intestinal epithelial cells.

RESULTS: More than 25 defense-related genes, including IRF1, SPINK4, ITLN1, OAS2, CIITA, HLA-DMB, HLA-DOB, PSMB9, TAP1, BTN3A1, and CX3CL1, were significantly upregulated in intestinal epithelial cells at active celiac disease. Of these genes, 70% were upregulated by interferon-γ via the IRF1 pathway. Most interestingly, IRF1 was also upregulated by celiac disease-associated bacteria. The NLRP6/8 inflammasome yielding CASP1 and biologically active interleukin-18, which induces interferon-γ in intraepithelial lymphocytes, was expressed in intestinal epithelial cells.

CONCLUSION: A key factor in the epithelial reaction in celiac disease appears to be over-expression of IRF1 that could be inherent and/or due to presence of undesirable microbes that act directly on IRF1. Dual activation of IRF1 and IRF1-regulated genes, both directly and via the interleukin-18 dependent inflammasome would drastically enhance the inflammatory response and lead to the pathological situation seen in active celiac disease.

National Category
Immunology
Identifiers
urn:nbn:se:umu:diva-139860 (URN)10.1371/journal.pone.0185025 (DOI)000411339900076 ()28934294 (PubMedID)2-s2.0-85029739563 (Scopus ID)
Available from: 2017-09-25 Created: 2017-09-25 Last updated: 2023-03-24Bibliographically approved
Soki, J., Hedberg, M., Patrick, S., Balint, B., Herczeg, R., Nagy, I., . . . Urban, E. (2016). Emergence and evolution of an international cluster of MDR Bacteroides fragilis isolates. Journal of Antimicrobial Chemotherapy, 71(9), 2441-2448
Open this publication in new window or tab >>Emergence and evolution of an international cluster of MDR Bacteroides fragilis isolates
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2016 (English)In: Journal of Antimicrobial Chemotherapy, ISSN 0305-7453, E-ISSN 1460-2091, Vol. 71, no 9, p. 2441-2448Article in journal (Refereed) Published
Abstract [en]

The aim of this study was to examine the antibiotic resistance profiles, antibiotic resistance mechanisms and possible 'clonal' nature of some MDR Bacteroides fragilis strains that simultaneously harboured cfiA, nimB, IS1186 and IS4351. Antibiotic susceptibilities were determined by Etests and antibiotic resistance genes and different genetic elements were detected by applying PCR methods. The environments of the cfiA and nimB genes were also determined by sequencing. The transferability of the cfiA, nimB and tet(Q) genes was tested by conjugation. The genetic relatedness of the test strains was tested by ERIC-PCR or PFGE. The complete genome sequences of two strains (B. fragilis BF8 and O:21) were determined by next-generation sequencing. Most of the seven B. fragilis strains tested displayed multidrug resistance phenotypes; five strains were resistant to at least five types of antibiotics. Besides the common genetic constitution, ERIC-PCR implied high genetic relatedness. Similarities in some of the antibiotic resistance mechanisms [carbapenems (cfiA) and metronidazole (nimB)] also confirmed their common origin, but some other resistance mechanisms {MLSB [erm(F)] and tetracycline [tet(Q)]} and PFGE typing revealed differences. In B. fragilis BF8 and O:21, erm(F) and tet(X) genes were found with IS4351 borders, thus constituting Tn4351. All the strains were tet(Q) positive and transferred this gene in conjugation experiments, but not the cfiA and nimB genes. An international cluster of MDR B. fragilis strains has been identified and characterized. This 'clone' may have emerged early in the evolution of division II B. fragilis strains, which was suggested by the low-complexity ERIC profiles and differences in the PFGE patterns.

National Category
Microbiology in the medical area
Identifiers
urn:nbn:se:umu:diva-127971 (URN)10.1093/jac/dkw175 (DOI)000383911600011 ()27246231 (PubMedID)2-s2.0-85011270550 (Scopus ID)
Available from: 2016-12-13 Created: 2016-11-21 Last updated: 2023-03-23Bibliographically approved
Sjöberg, V., Sandström, O., Hedberg, M., Hammarström, S., Hernell, O. & Hammarström, M.-L. (2013). Intestinal T-cell responses in celiac disease: impact of celiac disease associated bacteria. PLOS ONE, 8(1), e53414
Open this publication in new window or tab >>Intestinal T-cell responses in celiac disease: impact of celiac disease associated bacteria
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2013 (English)In: PLOS ONE, E-ISSN 1932-6203, Vol. 8, no 1, p. e53414-Article in journal (Refereed) Published
Abstract [en]

A hallmark of active celiac disease (CD), an inflammatory small-bowel enteropathy caused by permanent intolerance to gluten, is cytokine production by intestinal T lymphocytes. Prerequisites for contracting CD are that the individual carries the MHC class II alleles HLA-DQ2 and/or HLA-DQ8 and is exposed to gluten in the diet. Dysbiosis in the resident microbiota has been suggested to be another risk factor for CD. In fact, rod shaped bacteria adhering to the small intestinal mucosa were frequently seen in patients with CD during the "Swedish CD epidemic" and bacterial candidates could later be isolated from patients born during the epidemic suggesting long-lasting changes in the gut microbiota. Interleukin-17A (IL-17A) plays a role in both inflammation and anti-bacterial responses. In active CD IL-17A was produced by both CD8(+) T cells (Tc17) and CD4(+) T cells (Th17), with intraepithelial Tc17 cells being the dominant producers. Gluten peptides as well as CD associated bacteria induced IL-17A responses in ex vivo challenged biopsies from patients with inactive CD. The IL-17A response was suppressed in patients born during the epidemic when a mixture of CD associated bacteria was added to gluten, while the reverse was the case in patients born after the epidemic. Under these conditions Th17 cells were the dominant producers. Thus Tc17 and Th17 responses to gluten and bacteria seem to pave the way for the chronic disease with interferon-γ-production by intraepithelial Tc1 cells and lamina propria Th1 cells. The CD associated bacteria and the dysbiosis they might cause in the resident microbiota may be a risk factor for CD either by directly influencing the immune responses in the mucosa or by enhancing inflammatory responses to gluten.

Place, publisher, year, edition, pages
PLoS, Public Library of Science, 2013
National Category
Pediatrics Immunology in the medical area
Identifiers
urn:nbn:se:umu:diva-64525 (URN)10.1371/journal.pone.0053414 (DOI)000313551500069 ()23326425 (PubMedID)2-s2.0-84872227488 (Scopus ID)
Available from: 2013-01-31 Created: 2013-01-31 Last updated: 2023-03-24Bibliographically approved
Hedberg, M. E., Israelsson, A., Moore, E. R. B., Svensson-Stadler, L., Wai, S. N., Pietz, G., . . . Hammarstrom, S. (2013). Prevotella jejuni sp nov., isolated from the small intestine of a child with coeliac disease. International Journal of Systematic and Evolutionary Microbiology, 63(11), 4218-4223
Open this publication in new window or tab >>Prevotella jejuni sp nov., isolated from the small intestine of a child with coeliac disease
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2013 (English)In: International Journal of Systematic and Evolutionary Microbiology, ISSN 1466-5026, E-ISSN 1466-5034, Vol. 63, no 11, p. 4218-4223Article in journal (Refereed) Published
Abstract [en]

Five obligately anaerobic, Gram-stain-negative, saccharolytic and proteolytic, non-spore-forming bacilli (strains CD3 :27, CD3 :28(T), CD3 :33, CD3 :32 and CD3 :34) are described. All five strains were isolated from the small intestine of a female child with coeliac disease. Cells of the five strains were short rods or coccoid cells with longer filamentous forms seen sporadically. The organisms produced acetic acid and succinic acid as major metabolic end products. Phylogenetic analysis based on comparative 16S rRNA gene sequence analysis revealed close relationships between CD3 : 27, CD3 :28(T) and CD3 :33, between CD3 :32 and Prevotella histicola CCUG 55407(T), and between CD3 :34 and Prevotella melaninogenica CCUG 4944B(T). Strains CD3 : 27, CD3 :28(T) and CD3 :33 were clearly different from all recognized species within the genus Prevotella and related most closely to but distinct from P. melaninogenica. Based on 16S rRNA, RNA polymerase) beta-subunit (rpoB) and 60 kDa chaperonin protein subunit (cpn60) gene sequencing, and phenotypic, chemical and biochemical properties, strains CD3 :27, CD3 :28(T) and CD3 :33 are considered to represent a novel species within the genus Prevotella, for which the name Prevotella jejuni sp. nov. is proposed. Strain CD3 : 28(T) (=CCUG 60371(T)=DSM 26989(T)) is the type strain of the proposed novel species. All five strains were able to form homologous aggregates, in which tube-like structures were connecting individual bacteria cells. The five strains were able to bind to human intestinal carcinoma cell lines at 37 degrees C.

National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
urn:nbn:se:umu:diva-84790 (URN)10.1099/ijs.0.052647-0 (DOI)000328666600045 ()2-s2.0-84887049666 (Scopus ID)
Available from: 2014-01-28 Created: 2014-01-20 Last updated: 2023-03-24Bibliographically approved
Hedberg, M. E., Moore, E. R., Svensson-Stadler, L., Hörstedt, P., Baranov, V., Hernell, O., . . . Hammarström, M.-L. (2012). Lachnoanaerobaculum a new genus in Lachnospiraceae; characterization of Lachnoanaerobaculum umeaense gen. nov., sp. nov., isolated from human small intestine, Lachnoanaerobaculum orale gen. nov., sp. nov., isolated from saliva and reclassification of Eubacterium saburreum (Prevot) Holdeman and Moore 1970 as Lachnoanaerobaculum saburreum comb. nov.. International Journal of Systematic and Evolutionary Microbiology, 62(11), 2685-2690
Open this publication in new window or tab >>Lachnoanaerobaculum a new genus in Lachnospiraceae; characterization of Lachnoanaerobaculum umeaense gen. nov., sp. nov., isolated from human small intestine, Lachnoanaerobaculum orale gen. nov., sp. nov., isolated from saliva and reclassification of Eubacterium saburreum (Prevot) Holdeman and Moore 1970 as Lachnoanaerobaculum saburreum comb. nov.
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2012 (English)In: International Journal of Systematic and Evolutionary Microbiology, ISSN 1466-5026, E-ISSN 1466-5034, Vol. 62, no 11, p. 2685-2690Article in journal (Refereed) Published
Abstract [en]

Two new obligately anaerobic Gram-positive, saccharolytic and non-proteolytic spore-forming bacilli (strain CD3:22 and N1) are described. Strain CD3:22 was isolated from a biopsy of the small intestine of a child with celiac disease and strain N1 from the saliva of a healthy young man. The cells of both strains were observed to be filamentous with lengths of approximately 5 to >20 µm, some of them curving and with swellings. The novel organisms produced H2S, NH3, butyric acid and acetic acid as major metabolic end products. Phylogenetic analyses, based on comparative 16S rRNA gene sequencing, revealed close relationships (98 % sequence similarity) between the two isolates, as well as the type strain of Eubacterium saburreum CCUG 28089T and four other Lachnospiraceae bacterium/E. saburreum-like organisms. This group of bacteria were clearly different from any of the 19 known genera in the family Lachnospiraceae. While Eubacterium spp. are reported to be non-spore-forming, reanalysis of E. saburreum CCUG 28089T confirmed that the bacterium, indeed, is able to form spores. Based on 16S rRNA gene sequencing, phenotypic and biochemical properties, CD3:22 (CCUG 58757T) and N1 (CCUG 60305T) represent new species of a new and distinct genus, named Lachnoanaerobaculum, in the family Lachnospiraceae [within the order Clostridiales, class Clostridia, phylum Firmicutes]. Strain CD3:22 is the type strain of the type species, Lachnoanaerobaculum umeaense gen. nov., sp. nov., of the proposed new genus. Strain N1 is the type strain of the species, Lachnoanaerobaculum orale gen. nov., sp. nov. Moreover, E. saburreum CCUG 28089T is reclassified as Lachnoanaerobaculum saburreum comb. nov.

National Category
Microbiology
Identifiers
urn:nbn:se:umu:diva-51168 (URN)10.1099/ijs.0.033613-0 (DOI)22228654 (PubMedID)2-s2.0-84868311393 (Scopus ID)
Funder
EU, FP7, Seventh Framework Programme, 222720Swedish Research Council
Available from: 2012-01-11 Created: 2012-01-11 Last updated: 2023-03-24Bibliographically approved
Hedberg, M., Hammarström, M.-L., Hernell, O., Baranov, V., Wai, S. N., Moore, E. & Hammarström, S. (2010). Clostridiales bacterium CD3:22-an anaerobic spore-forming bacterium isolated from small intestine in a celiac disease patient.
Open this publication in new window or tab >>Clostridiales bacterium CD3:22-an anaerobic spore-forming bacterium isolated from small intestine in a celiac disease patient
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2010 (English)Report (Other academic)
National Category
Clinical Medicine
Identifiers
urn:nbn:se:umu:diva-35386 (URN)
Note
Kompletteras 2012-09Available from: 2010-08-16 Created: 2010-08-16 Last updated: 2018-06-08Bibliographically approved
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