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Ekström, Karin
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Publications (4 of 4) Show all publications
Ryder, E., Ashburner, M., Bautista-Llacer, R., Drummond, J., Webster, J., Johnson, G., . . . Russell, S. (2007). The DrosDel deletion collection: A Drosophila genomewide chromosomal deficiency resource. Genetics, 177(1), 615-629
Open this publication in new window or tab >>The DrosDel deletion collection: A Drosophila genomewide chromosomal deficiency resource
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2007 (English)In: Genetics, ISSN 0016-6731, E-ISSN 1943-2631, Vol. 177, no 1, p. 615-629Article in journal (Refereed) Published
Abstract [en]

We describe a second-generation deficiency kit for Drosophila melanogaster composed of molecularly mapped deletions on an isogenic background, covering 77% of the Release 5.1 genome. Using a previously reported collection of FRT-bearing P-element insertions, we have generated 655 new deletions and verified a set of 209 deletion-bearing fly stocks. In addition to deletions, we demonstrate how the P elements may also be used to generate a set of custom inversions and duplications, particularly useful for balancing difficult regions of the genome carrying haplo-insufficient loci. We describe a simple computational resource that facilitatesselection of appropriate elements for generating custom deletions. Finally, we provide a computational resource that facilitates selection of other mapped FRT-bearing elements that, when combined with the DrosDel collection, can theoretically generate over half a million precisely mapped deletions.

Place, publisher, year, edition, pages
Austin, Tex.: Genetics Society of America, 2007
Identifiers
urn:nbn:se:umu:diva-16815 (URN)10.1534/genetics.107.076216 (DOI)17720900 (PubMedID)2-s2.0-35048876714 (Scopus ID)
Available from: 2007-10-12 Created: 2007-10-12 Last updated: 2023-03-24Bibliographically approved
Ryder, E., Blows, F., Ashburner, M., Bautista-Llacer, R., Coulson, D., Drummond, J., . . . Russell, S. (2004). The DrosDel collection: a set of P-element insertions for generating custom chromosomal aberrations in Drosophila melanogaster.. Genetics, 167(2), 797-813
Open this publication in new window or tab >>The DrosDel collection: a set of P-element insertions for generating custom chromosomal aberrations in Drosophila melanogaster.
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2004 (English)In: Genetics, ISSN 0016-6731, Vol. 167, no 2, p. 797-813Article in journal (Refereed) Published
Abstract [en]

We describe a collection of P-element insertions that have considerable utility for generating custom chromosomal aberrations in Drosophila melanogaster. We have mobilized a pair of engineered P elements, p[RS3] and p[RS5], to collect 3243 lines unambiguously mapped to the Drosophila genome sequence. The collection contains, on average, an element every 35 kb. We demonstrate the utility of the collection for generating custom chromosomal deletions that have their end points mapped, with base-pair resolution, to the genome sequence. The collection was generated in an isogenic strain, thus affording a uniform background for screens where sensitivity to genetic background is high. The entire collection, along with a computational and genetic toolbox for designing and generating custom deletions, is publicly available. Using the collection it is theoretically possible to generate >12,000 deletions between 1 bp and 1 Mb in size by simple eye color selection. In addition, a further 37,000 deletions, selectable by molecular screening, may be generated. We are now using the collection to generate a second-generation deficiency kit that is precisely mapped to the genome sequence.

Keywords
Animals, Chromosome Aberrations, DNA Transposable Elements/*genetics, Drosophila melanogaster/*genetics, Genetic Techniques, Mutagenesis; Insertional/methods
Identifiers
urn:nbn:se:umu:diva-16827 (URN)15238529 (PubMedID)2-s2.0-2942743598 (Scopus ID)
Available from: 2007-10-12 Created: 2007-10-12 Last updated: 2023-03-24Bibliographically approved
Rasmuson-Lestander, Å. & Ekström, K. (1996). Genetic and molecular analysis of a set of unstable white mutants in Drosophila melanogaster.. Genetica, 98(2), 179-92
Open this publication in new window or tab >>Genetic and molecular analysis of a set of unstable white mutants in Drosophila melanogaster.
1996 (English)In: Genetica, ISSN 0016-6707, Vol. 98, no 2, p. 179-92Article in journal (Refereed) Published
Keywords
Animals, Blotting; Southern, Chromosome Mapping, DNA Probes, Drosophila melanogaster/*genetics, Female, Gene Deletion, Genes; Insect, Genomic Library, In Situ Hybridization, Male, Mutation, Phenotype, Restriction Mapping, X Chromosome
Identifiers
urn:nbn:se:umu:diva-17916 (URN)8976064 (PubMedID)
Available from: 2007-11-23 Created: 2007-11-23 Last updated: 2018-06-09Bibliographically approved
Jacobsson, L., Skottheim Honn, J., Ekström, K. & Rasmuson-Lestander, Å.Empty spiracles represses twin-of-eyeless in the Drosophila embryonic head.
Open this publication in new window or tab >>Empty spiracles represses twin-of-eyeless in the Drosophila embryonic head
(English)Manuscript (preprint) (Other academic)
Abstract [en]

The specification of the eye-antennal disc primordium in the Drosophila embryo requires the expression of two paralogous Pax6 genes: twin of eyeless (toy) and eyeless (ey). toy is considered to be the first eye specification gene expressed in the regulatory network that governs eye formation and the gene that, in turn, activates eyeless. What regulates toy expression is, however, still unclear. We show, by misexpression and mutant analysis, that the head-specific gene empty spiracles alters the expression pattern of Toy in the head region around the visual primordia.

Keywords
Drosophila, empty spiracles, eye development, Pax6, twin-of-eyeless
National Category
Natural Sciences
Research subject
Molecular Biology
Identifiers
urn:nbn:se:umu:diva-50725 (URN)
Available from: 2011-12-20 Created: 2011-12-19 Last updated: 2018-06-08Bibliographically approved

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