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Isaksson, Andreas
Publications (7 of 7) Show all publications
Yu, J.-G., Isaksson, A., Rova, A., Tegner, Y., Eriksson, A. & Malm, C. B. (2020). Potential effects of long-term abuse of anabolic androgen steroids on human skeletal muscle. Journal of Sports Medicine and Physical Fitness, 60(7), 1040-1048
Open this publication in new window or tab >>Potential effects of long-term abuse of anabolic androgen steroids on human skeletal muscle
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2020 (English)In: Journal of Sports Medicine and Physical Fitness, ISSN 0022-4707, E-ISSN 1827-1928, Vol. 60, no 7, p. 1040-1048Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: We have previously evaluated muscle functions and morphology in power athletes of long term (5 to15 years) abuse of anabolic androgen steroids (AAS; Doped) and in clean power athletes (Clean), and observed significant improvements in both muscle morphology and muscle functions in Doped. To our knowledge, the effects of long term AAS abuse on human muscle protein profile have never been studied.

METHODS: The study examined further the muscle biopsies using a two-dimensional difference gel electrophoresis (2D DIGE) for proteomic screening and protein expression. Cellular localization/distribution of specific proteins identified by proteomic analysis was examined using immunohistochemistry (IHC).

RESULTS: Different protein profiles were observed between Doped and Clean, and a valid orthogonal projection of latent structure discriminant analysis model was built (N.=16, x=5, R-2=0.88/Q(2)=0.84, P=0.0005), which separated Doped from Clean. Liquid chromatography followed by tandem spectrometry identified 14 protein spots (representing nine different proteins) of significant difference in relative quantity (P<0.05), of which nine spots were down-regulated in Doped compared with Clean. IHC revealed no significant alteration in cellular localization in phosphoglucomutase-1 and heat shock protein beta-1, but indeed in two reference proteins desmin and F-actin in Doped.

CONCLUSIONS: Long term abuse of AAS in combination with training is potentially associated with alterations in skeletal muscle protein profile and protein expression, and structural proteins rather than non-structural proteins are preferentially affected in cellular localization/distribution.

Place, publisher, year, edition, pages
Edizioni Minerva Medica, 2020
Keywords
Doping in sports, Testosterone congeners, Skeletal muscle, Proteomics, Gene expression
National Category
Sport and Fitness Sciences
Identifiers
urn:nbn:se:umu:diva-173634 (URN)10.23736/S0022-4707.20.10443-2 (DOI)000545319500013 ()32343079 (PubMedID)2-s2.0-85087433642 (Scopus ID)
Available from: 2020-07-21 Created: 2020-07-21 Last updated: 2023-03-23Bibliographically approved
Otten, J., Stomby, A., Waling, M., Isaksson, A., Söderström, I., Ryberg, M., . . . Olsson, T. (2018). A heterogeneous response of liver and skeletal muscle fat to the combination of a Paleolithic diet and exercise in obese individuals with type 2 diabetes: a randomised controlled trial. Diabetologia, 61(7), 1548-1559
Open this publication in new window or tab >>A heterogeneous response of liver and skeletal muscle fat to the combination of a Paleolithic diet and exercise in obese individuals with type 2 diabetes: a randomised controlled trial
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2018 (English)In: Diabetologia, ISSN 0012-186X, E-ISSN 1432-0428, Vol. 61, no 7, p. 1548-1559Article in journal (Refereed) Published
Abstract [en]

Aims/hypothesis: The aim of the study was to investigate ectopic fat deposition and insulin sensitivity, in a parallel single-blinded randomised controlled trial, comparing Paleolithic diet alone with the combination of Paleolithic diet and exercise in individuals with type 2 diabetes. Methods: Thirty-two individuals with type 2 diabetes with BMI 25-40 kg/m(2) and 30-70 years of age followed a Paleolithic diet ad libitum for 12 weeks. In addition, study participants were randomised by computer program to either supervised combined exercise training (PD-EX group) or standard care exercise recommendations (PD group). Staff performing examinations and assessing outcomes were blinded to group assignment. Thirteen participants were analysed in each group: hepatic and peripheral insulin sensitivity were measured using the hyperinsulinaemic-euglycaemic clamp technique combined with [6,6-H-2(2)]glucose infusion, and liver fat was assessed by proton magnetic resonance spectroscopy; both analyses were secondary endpoints. Intramyocellular lipid (IMCL) content was measured by magnetic resonance spectroscopy as a secondary analysis. All examinations were performed at Umca University Hospital, Umca, Sweden. Results: Both study groups showed a median body weight loss of 7 kg. Fat mass decreased by 5.7 kg in the PD group and by 6.5 kg in the PD-EX group. Maximum oxygen uptake increased in the PD-EX group only. Liver fat showed a consistent reduction (74% decrease) in the PD group, while the response in the PD-EX group was heterogeneous (p < 0.05 for the difference between groups). IMCL content of the soleus muscle decreased by 40% in the PD group and by 22% in the PD-EX group (p < 0.05 for the difference between groups). Both groups improved their peripheral and adipose tissue insulin sensitivity, but not their hepatic insulin sensitivity. Plasma fetuin-A decreased by 11% in the PD group (p < 0.05) and remained unchanged in the PD-EX group. Liver fat changes during the intervention were correlated with changes in fetuin-A (r(S) = 0.63, p < 0.01). Participants did not report any important adverse events caused by the intervention. Conclusions/interpretation: A Paleolithic diet reduced liver fat and IMCL content, while there was a tissue-specific heterogeneous response to added exercise training.

Place, publisher, year, edition, pages
Springer, 2018
Keywords
Exercise, Hyperinsulinaemic-euglycaemic clamp, Insulin sensitivity, Intramyocellular fat, Liver fat, Nutrition, Obesity, Paleolithic diet, Proton magnetic resonance spectroscopy, Weight loss
National Category
Endocrinology and Diabetes
Identifiers
urn:nbn:se:umu:diva-150764 (URN)10.1007/s00125-018-4618-y (DOI)000434250500007 ()29696296 (PubMedID)2-s2.0-85046029986 (Scopus ID)
Available from: 2018-08-27 Created: 2018-08-27 Last updated: 2024-07-02Bibliographically approved
Otten, J., Stomby, A., Waling, M., Isaksson, A., Tellström, A., Lundin-Olsson, L., . . . Olsson, T. (2017). Benefits of a Paleolithic diet with and without supervised exercise on fat mass, insulin sensitivity, and glycemic control: a randomized controlled trial in individuals with type 2 diabetes. Diabetes/Metabolism Research Reviews, 33(1), Article ID e2828.
Open this publication in new window or tab >>Benefits of a Paleolithic diet with and without supervised exercise on fat mass, insulin sensitivity, and glycemic control: a randomized controlled trial in individuals with type 2 diabetes
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2017 (English)In: Diabetes/Metabolism Research Reviews, ISSN 1520-7552, E-ISSN 1520-7560, Vol. 33, no 1, article id e2828Article in journal (Refereed) Published
Abstract [en]

Background

Means to reduce future risk for cardiovascular disease in subjects with type 2 diabetes are urgently needed.

Methods

Thirty-two patients with type 2 diabetes (age 59 ± 8 years) followed a Paleolithic diet for 12 weeks. Participants were randomized to either standard care exercise recommendations (PD) or 1-h supervised exercise sessions (aerobic exercise and resistance training) three times per week (PD-EX).

Results

For the within group analyses, fat mass decreased by 5.7 kg (IQR: −6.6, −4.1; p < 0.001) in the PD group and by 6.7 kg (−8.2, −5.3; p < 0.001) in the PD-EX group. Insulin sensitivity (HOMA-IR) improved by 45% in the PD (p < 0.001) and PD-EX (p < 0.001) groups. HbA1c decreased by 0.9% (−1.2, −0.6; p < 0.001) in the PD group and 1.1% (−1.7, −0.7; p < 0.01) in the PD-EX group. Leptin decreased by 62% (p < 0.001) in the PD group and 42% (p < 0.001) in the PD-EX group. Maximum oxygen uptake increased by 0.2 L/min (0.0, 0.3) in the PD-EX group, and remained unchanged in the PD group (p < 0.01 for the difference between intervention groups). Male participants decreased lean mass by 2.6 kg (−3.6, −1.3) in the PD group and by 1.2 kg (−1.3, 1.0) in the PD-EX group (p < 0.05 for the difference between intervention groups).

Conclusions

A Paleolithic diet improves fat mass and metabolic balance including insulin sensitivity, glycemic control, and leptin in subjects with type 2 diabetes. Supervised exercise training may not enhance the effects on these outcomes, but preserves lean mass in men and increases cardiovascular fitness.

Keywords
type 2 diabetes, Paleolithic diet, diet intervention, exercise, glycosyl-ated haemoglobin A, insulin sensitivity, leptin
National Category
Endocrinology and Diabetes
Identifiers
urn:nbn:se:umu:diva-126188 (URN)10.1002/dmrr.2828 (DOI)000397102800010 ()2-s2.0-84976866496 (Scopus ID)
Available from: 2016-10-03 Created: 2016-10-03 Last updated: 2024-07-02Bibliographically approved
Otten, J., Stomby, A., Waling, M., Isaksson, A., Söderström, I., Ryberg, M., . . . Olsson, T. (2017). Exercise training reverses the effect of a Paleolithic diet on liver fat and intramyocellular lipid content in patients with type 2 diabetes. In: : . Paper presented at Cell Symposia Exercise Metabolism, 21-23 May, 2017, Gothenburg, Sweden.
Open this publication in new window or tab >>Exercise training reverses the effect of a Paleolithic diet on liver fat and intramyocellular lipid content in patients with type 2 diabetes
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2017 (English)Conference paper, Poster (with or without abstract) (Refereed)
National Category
Endocrinology and Diabetes
Identifiers
urn:nbn:se:umu:diva-135408 (URN)
Conference
Cell Symposia Exercise Metabolism, 21-23 May, 2017, Gothenburg, Sweden
Available from: 2017-05-26 Created: 2017-05-26 Last updated: 2024-07-02Bibliographically approved
Otten, J., Stomby, A., Waling, M., Isaksson, A., Söderström, I., Ryberg, M., . . . Olsson, T. (2017). Exercise training reverses the effect of a Paleolithic diet on liver fat and intramyocellular lipid content in patients with type 2 diabetes. In: : . Paper presented at Endokrindagarna, 1-3 februari 2017, Uppsala, Sweden.
Open this publication in new window or tab >>Exercise training reverses the effect of a Paleolithic diet on liver fat and intramyocellular lipid content in patients with type 2 diabetes
Show others...
2017 (English)Conference paper, Poster (with or without abstract) (Refereed)
National Category
Endocrinology and Diabetes
Identifiers
urn:nbn:se:umu:diva-135409 (URN)
Conference
Endokrindagarna, 1-3 februari 2017, Uppsala, Sweden
Available from: 2017-05-26 Created: 2017-05-26 Last updated: 2024-07-02Bibliographically approved
Malm, C. & Isaksson, A. (2017). Idrott: en viktig faktor för fysisk och psykisk hälsa. In: JohanFaskunger, Paul Sjöblom (Ed.), Idrottens samhällsnytta: en vetenskaplig översikt av idrottsrörelsens mervärden för individ och samhälle (pp. 21-39). Stockholm: Riksidrottsförbundet
Open this publication in new window or tab >>Idrott: en viktig faktor för fysisk och psykisk hälsa
2017 (Swedish)In: Idrottens samhällsnytta: en vetenskaplig översikt av idrottsrörelsens mervärden för individ och samhälle / [ed] JohanFaskunger, Paul Sjöblom, Stockholm: Riksidrottsförbundet , 2017, , p. 229p. 21-39Chapter in book (Other academic)
Place, publisher, year, edition, pages
Stockholm: Riksidrottsförbundet, 2017. p. 229
Series
FoU-rapport ; 2017:1
National Category
Sport and Fitness Sciences
Identifiers
urn:nbn:se:umu:diva-153080 (URN)978-91-87385-19-3 (ISBN)
Available from: 2018-11-06 Created: 2018-11-06 Last updated: 2024-07-02Bibliographically approved
Otten, J., Stomby, A., Waling, M., Isaksson, A., Tellström, A., Lundin-Olsson, L., . . . Olsson, T. (2016). Effects of a Paleolithic diet with and without supervised exercise on fat mass, insulin sensitivity, and glycemic control: a randomized controlled trial in individuals with type 2 diabetes. In: : . Paper presented at Endokrindagarna, Stockholm, Sweden.
Open this publication in new window or tab >>Effects of a Paleolithic diet with and without supervised exercise on fat mass, insulin sensitivity, and glycemic control: a randomized controlled trial in individuals with type 2 diabetes
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2016 (Swedish)Conference paper, Poster (with or without abstract) (Refereed)
National Category
Endocrinology and Diabetes
Identifiers
urn:nbn:se:umu:diva-135410 (URN)
Conference
Endokrindagarna, Stockholm, Sweden
Available from: 2017-05-26 Created: 2017-05-26 Last updated: 2024-07-02Bibliographically approved
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