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Melguizo-Sanchis, Dario
Publications (2 of 2) Show all publications
Malla, S., Bhattarai, D. P., Groza, P., Melguizo-Sanchis, D., Atanasoai, I., Martinez Gamero, C., . . . Aguilo, F. (2022). ZFP207 sustains pluripotency by coordinating OCT4 stability, alternative splicing and RNA export. EMBO Reports, 23(3), Article ID e53191.
Open this publication in new window or tab >>ZFP207 sustains pluripotency by coordinating OCT4 stability, alternative splicing and RNA export
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2022 (English)In: EMBO Reports, ISSN 1469-221X, E-ISSN 1469-3178, Vol. 23, no 3, article id e53191Article in journal (Refereed) Published
Abstract [en]

The pluripotent state is not solely governed by the action of the core transcription factors OCT4, SOX2, and NANOG, but also by a series of co-transcriptional and post-transcriptional events, including alternative splicing (AS) and the interaction of RNA-binding proteins (RBPs) with defined subpopulations of RNAs. Zinc Finger Protein 207 (ZFP207) is an essential transcription factor for mammalian embryonic development. Here, we employ multiple functional analyses to characterize its role in mouse embryonic stem cells (ESCs). We find that ZFP207 plays a pivotal role in ESC maintenance, and silencing of Zfp207 leads to severe neuroectodermal differentiation defects. In striking contrast to human ESCs, mouse ZFP207 does not transcriptionally regulate neuronal and stem cell-related genes but exerts its effects by controlling AS networks and by acting as an RBP. Our study expands the role of ZFP207 in maintaining ESC identity, and underscores the functional versatility of ZFP207 in regulating neural fate commitment.

Place, publisher, year, edition, pages
John Wiley & Sons, 2022
National Category
Cell and Molecular Biology
Identifiers
urn:nbn:se:umu:diva-191672 (URN)10.15252/embr.202153191 (DOI)000743102200001 ()35037361 (PubMedID)2-s2.0-85122763926 (Scopus ID)
Available from: 2022-01-21 Created: 2022-01-21 Last updated: 2024-04-08Bibliographically approved
Malla, S., Melguizo-Sanchis, D. & Aguilo, F. (2019). Steering pluripotency and differentiation with N6-methyladenosine RNA modification. Biochimica et Biophysica Acta. Gene Regulatory Mechanisms, 1862(3), 394-402
Open this publication in new window or tab >>Steering pluripotency and differentiation with N6-methyladenosine RNA modification
2019 (English)In: Biochimica et Biophysica Acta. Gene Regulatory Mechanisms, ISSN 1874-9399, E-ISSN 1876-4320, Vol. 1862, no 3, p. 394-402Article in journal (Refereed) Published
Abstract [en]

Chemical modifications of RNA provide a direct and rapid way to modulate the existing transcriptome, allowing the cells to adapt rapidly to the changing environment. Among these modifications, N6-methyladenosine (m6A) has recently emerged as a widely prevalent mark of messenger RNA in eukaryotes, linking external stimuli to an intricate network of transcriptional, post-transcriptional and translational processes. m6A modification modulates a broad spectrum of biochemical processes, including mRNA decay, translation and splicing. Both m6A modification and the enzymes that control m6A metabolism are essential for normal development. In this review, we summarized the most recent findings on the role of m6A modification in maintenance of the pluripotency of embryonic stem cells (ESCs), cell fate specification, the reprogramming of somatic cells into induced pluripotent stem cells (iPSCs), and differentiation of stem and progenitor cells.

Place, publisher, year, edition, pages
Elsevier, 2019
Keywords
Adipogenesis, Cellular differentiation, Embryonic stem cell, Epitranscriptomics, Hematopoietic stem cell, Induced pluripotent stem cell, METTL3, Myogenesis, N(6)-methyladenosine, Neurogenesis, RNA methylation, Spermatogenesis
National Category
Cell and Molecular Biology
Identifiers
urn:nbn:se:umu:diva-153977 (URN)10.1016/j.bbagrm.2018.10.013 (DOI)000462104500017 ()30412796 (PubMedID)2-s2.0-85056392321 (Scopus ID)
Funder
Knut and Alice Wallenberg FoundationVästerbotten County CouncilThe Kempe Foundations, JCK-1723.1Swedish Research Council, 2017-01636
Note

This article is part of a Special Issue entitled: mRNA modifications in gene expression control edited by Dr. Soller Matthias and Dr. Fray Rupert

Available from: 2018-12-10 Created: 2018-12-10 Last updated: 2023-03-24Bibliographically approved
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