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Hall, Hardy
Publications (2 of 2) Show all publications
Pitsili, E., Rodriguez-Trevino, R., Ruiz-Solani, N., Demir, F., Kastanaki, E., Dambire, C., . . . Coll, N. S. (2023). A phloem-localized Arabidopsis metacaspase (AtMC3) improves drought tolerance. New Phytologist, 239, 1281-1299
Open this publication in new window or tab >>A phloem-localized Arabidopsis metacaspase (AtMC3) improves drought tolerance
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2023 (English)In: New Phytologist, ISSN 0028-646X, E-ISSN 1469-8137, Vol. 239, p. 1281-1299Article in journal (Refereed) Published
Abstract [en]

Increasing drought phenomena pose a serious threat to agricultural productivity. Although plants have multiple ways to respond to the complexity of drought stress, the underlying mechanisms of stress sensing and signaling remain unclear. The role of the vasculature, in particular the phloem, in facilitating inter-organ communication is critical and poorly understood. Combining genetic, proteomic and physiological approaches, we investigated the role of AtMC3, a phloem-specific member of the metacaspase family, in osmotic stress responses in Arabidopsis thaliana. Analyses of the proteome in plants with altered AtMC3 levels revealed differential abundance of proteins related to osmotic stress pointing into a role of the protein in water-stress-related responses. Overexpression of AtMC3 conferred drought tolerance by enhancing the differentiation of specific vascular tissues and maintaining higher levels of vascular-mediated transportation, while plants lacking the protein showed an impaired response to drought and inability to respond effectively to the hormone abscisic acid. Overall, our data highlight the importance of AtMC3 and vascular plasticity in fine-tuning early drought responses at the whole plant level without affecting growth or yield.

Place, publisher, year, edition, pages
John Wiley & Sons, 2023
Keywords
abscisic acid, Arabidopsis thaliana, drought, hypoxia, metacaspases, osmotic stress, phloem
National Category
Botany
Identifiers
urn:nbn:se:umu:diva-210685 (URN)10.1111/nph.19022 (DOI)001010896700001 ()37320971 (PubMedID)2-s2.0-85161826198 (Scopus ID)
Available from: 2023-06-26 Created: 2023-06-26 Last updated: 2023-09-22Bibliographically approved
Escamez, S., André, D., Sztojka, B., Bollhöner, B., Hall, H., Berthet, B., . . . Tuominen, H. (2020). Cell Death in Cells Overlying Lateral Root Primordia Facilitates Organ Growth in Arabidopsis. Current Biology, 30(3), 455-464
Open this publication in new window or tab >>Cell Death in Cells Overlying Lateral Root Primordia Facilitates Organ Growth in Arabidopsis
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2020 (English)In: Current Biology, ISSN 0960-9822, E-ISSN 1879-0445, Vol. 30, no 3, p. 455-464Article in journal (Refereed) Published
Abstract [en]

Plant organ growth is widely accepted to be determined by cell division and cell expansion, but, unlike that in animals, the contribution of cell elimination has rarely been recognized. We investigated this paradigm during Arabidopsis lateral root formation, when the lateral root primordia (LRP) must traverse three overlying cell layers within the parent root. A subset of LRP-overlying cells displayed the induction of marker genes for cell types undergoing developmental cell death, and their cell death was detected by electron, confocal, and light sheet microscopy techniques. LRP growth was delayed in cell-deathdeficient mutants lacking the positive cell death regulator ORESARA1/ANAC092 (ORE1). LRP growth was restored in ore1-2 knockout plants by genetically inducing cell elimination in cells overlying the LRP or by physically killing LRP-overlying cells by ablation with optical tweezers. Our results support that, in addition to previously discovered mechanisms, cell elimination contributes to regulating lateral root emergence.

Place, publisher, year, edition, pages
Elsevier, 2020
National Category
Developmental Biology Other Physics Topics
Identifiers
urn:nbn:se:umu:diva-168335 (URN)10.1016/j.cub.2019.11.078 (DOI)000511287900024 ()31956028 (PubMedID)2-s2.0-85078465553 (Scopus ID)
Projects
Bio4Energy
Funder
Bio4Energy
Available from: 2020-02-24 Created: 2020-02-24 Last updated: 2023-03-24Bibliographically approved
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