Umeå University's logo

umu.sePublications
Change search
Link to record
Permanent link

Direct link
Publications (2 of 2) Show all publications
Renman, E., Ekici, R., Sundström, M. & Lejon, K. (2022). HSC70 is a novel binding partner involved in the capture of immunoglobulins on B cells in the NOD mouse. Autoimmunity, 55(8), 520-528
Open this publication in new window or tab >>HSC70 is a novel binding partner involved in the capture of immunoglobulins on B cells in the NOD mouse
2022 (English)In: Autoimmunity, ISSN 0891-6934, E-ISSN 1607-842X, Vol. 55, no 8, p. 520-528Article in journal (Refereed) Published
Abstract [en]

B cells have been shown to be essential for Type 1 diabetes development in the non-obese diabetic mouse, where their contribution as antigen presenting cells has been emphasised. Other important functions for B cells include surface capture of immunoglobulins and transportation of immune complexes, with subsequent endocytosis, antigen processing and antigen presentation. We have previously demonstrated that NOD B cells capture IgM and IgG immune complexes through an unknown surface molecule. In this study, we revealed the presumptive immunoglobulin-binding molecule to be HSC70. Moreover, we detected increased levels of HSC70 on NOD B cells. HSC70 has been shown to play a role in antigen processing and presentation as well as being important in several autoimmune diseases, including rheumatoid arthritis and systemic lupus erythematosus. Due to its protein stabilising properties, increased HSC70 could contribute to enhanced self-antigen collection and presentation and thereby contribute to the development of Type 1 diabetes.

Place, publisher, year, edition, pages
Taylor & Francis, 2022
Keywords
B lymphocyte, HSC70, immune complex, NOD mouse, Type 1 diabetes
National Category
Immunology in the medical area
Research subject
Immunology
Identifiers
urn:nbn:se:umu:diva-199900 (URN)10.1080/08916934.2022.2117307 (DOI)000855330200001 ()36120986 (PubMedID)2-s2.0-85138421853 (Scopus ID)
Funder
DiabetesfondenSwedish Child Diabetes Foundation
Available from: 2022-10-03 Created: 2022-10-03 Last updated: 2022-11-29Bibliographically approved
Renman, E., Brink, M., Ärlestig, L., Rantapää-Dahlqvist, S. & Lejon, K. (2021). Dysregulated microRNA expression in rheumatoid arthritis families-a comparison between rheumatoid arthritis patients, their first-degree relatives, and healthy controls. Clinical Rheumatology, 40(6), 2387-2394
Open this publication in new window or tab >>Dysregulated microRNA expression in rheumatoid arthritis families-a comparison between rheumatoid arthritis patients, their first-degree relatives, and healthy controls
Show others...
2021 (English)In: Clinical Rheumatology, ISSN 0770-3198, E-ISSN 1434-9949, Vol. 40, no 6, p. 2387-2394Article in journal (Refereed) Published
Abstract [en]

OBJECTIVE: Recent studies have demonstrated an altered expression of certain microRNAs in patients with rheumatoid arthritis (RA) as well as their first-degree relatives (FDRs) compared to healthy controls (HCs), suggesting a role of microRNA in the progression of the disease. To corroborate this, a set of well-characterized RA families originating from northern Sweden were analyzed for differential expression of a selected set of microRNAs.

METHOD: MicroRNA was isolated from frozen peripheral blood cells obtained from 21 different families and included 26 RA patients, 22 FDRs, and 21 HCs. Expression of the selected microRNAs miR-22-3p, miR-26b-5p, miR-34a-3p, miR-103a-3p, miR-142-3p, miR-146a-5p, miR-155, miR-346, and miR-451a was determined by a two-step quantitative real-time polymerase chain reaction (qRT-PCR). Statistical analysis including clinical variables was applied.

RESULTS: Out of the nine selected microRNAs that previously have been linked to RA, we confirmed four after adjusting for age and gender, i.e., miR-22-3p (p = 0.020), miR-26b-5p (p = 0.018), miR-142-3p (p = 0.005), and miR-155 (p = 0.033). Moreover, a significant trend with an intermediate microRNA expression in FDR was observed for the same four microRNAs. In addition, analysis of the effect of corticosteroid use showed modulation of miR-103a-3p expression.

CONCLUSIONS: We confirm that microRNAs seem to be involved in the development of RA, and that the expression pattern in FDR is partly overlapping with RA patients. The contribution of single microRNAs in relation to the complex network including all microRNAs and other molecules is still to be revealed. Key Points • Expression levels of miR-22-3p, miR-26b-5p, miR-142-3p, and miR-155 were significantly altered in RA patients compared to those in controls. • In first-degree relatives, a significant trend with an intermediate microRNA expression in FDR was observed for the same four microRNAs.

Place, publisher, year, edition, pages
Springer, 2021
Keywords
Autoimmunity, MicroRNA family, Rheumatoid arthritis, Sweden
National Category
Rheumatology and Autoimmunity
Identifiers
urn:nbn:se:umu:diva-176964 (URN)10.1007/s10067-020-05502-9 (DOI)000591447600001 ()33210166 (PubMedID)2-s2.0-85096216498 (Scopus ID)
Funder
Swedish Research Council, 201802551King Gustaf V Jubilee FundSwedish Rheumatism Association
Available from: 2020-11-23 Created: 2020-11-23 Last updated: 2023-03-24Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0002-2051-2973

Search in DiVA

Show all publications