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Kumari, Kanchan
Publications (7 of 7) Show all publications
Kar, A., Kumari, K., Mishra, S. K. & Subudhi, U. (2022). Self-assembled DNA nanostructure containing oncogenic miRNA-mediated cell proliferation by downregulation of FOXO1 expression. BMC Cancer, 22(1), Article ID 1332.
Open this publication in new window or tab >>Self-assembled DNA nanostructure containing oncogenic miRNA-mediated cell proliferation by downregulation of FOXO1 expression
2022 (English)In: BMC Cancer, ISSN 1471-2407, E-ISSN 1471-2407, Vol. 22, no 1, article id 1332Article in journal (Refereed) Published
Abstract [en]

FOXO1 transcription factor not only limits the cell cycle progression but also promotes cell death as a tumor suppressor protein. Though the expression of FOXO1 is largely examined in breast cancer, the regulation of FOXO1 by miRNA is yet to be explored. In the current study, self-assembled branched DNA (bDNA) nanostructures containing oncogenic miRNAs were designed and transfected to the MCF7 cell line to decipher the FOXO1 expression. bDNA containing oncogenic miRNAs 27a, 96, and 182 synergistically downregulate the expression of FOXO1 in MCF7 cells. The down-regulation is evident both in mRNA and protein levels suggesting that bDNA having miRNA sequences can selectively bind to mRNA and inhibit translation. Secondly, the downstream gene expression of p21 and p27 was also significantly downregulated in presence of miR-bDNA nanostructures. The cell proliferation activity was progressively increased in presence of miR-bDNA nanostructures which confirms the reduced tumor suppression activity of FOXO1 and the downstream gene expression. This finding can be explored to design novel bDNA structures which can downregulate the tumor suppressor proteins in normal cells and induce cell proliferation activity to identify early-phase markers of cancer.

Place, publisher, year, edition, pages
BioMed Central (BMC), 2022
Keywords
Breast cancer, DNA nanostructure, FOXO1, MCF7 cell line, miRNAs, P21, P27
National Category
Cancer and Oncology Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:umu:diva-202070 (URN)10.1186/s12885-022-10423-8 (DOI)000901744400006 ()36539739 (PubMedID)2-s2.0-85144301110 (Scopus ID)
Available from: 2023-01-03 Created: 2023-01-03 Last updated: 2023-09-05Bibliographically approved
Samal, R. R., Kumari, K., Sahoo, Y., Mishra, S. K. & Subudhi, U. (2021). Interaction of artemisinin protects the activity of antioxidant enzyme catalase: a biophysical study. International Journal of Biological Macromolecules, 172, 418-428
Open this publication in new window or tab >>Interaction of artemisinin protects the activity of antioxidant enzyme catalase: a biophysical study
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2021 (English)In: International Journal of Biological Macromolecules, ISSN 0141-8130, E-ISSN 1879-0003, Vol. 172, p. 418-428Article in journal (Refereed) Published
Abstract [en]

The major antioxidant enzyme catalase is downregulated and the enzyme activity is compromised in various disease conditions such as malarial and cancer. Hence, the restoration and protection of catalase is a promising therapeutic strategy in disease management. In the present study, for the first time we have demonstrated the protective role of well-known anti-malarial drug Artemisinin (ART) on the time and temperature-induced degradation of bovine liver catalase (BLC) activity. The findings at different time intervals and at higher temperature showed the protective role of ART on BLC activity. Molecular docking studies suggested specific binding of ART on BLC through heme group interface which was further supported by cyclic voltammetry and dynamic light scattering study. The stabilization of BLC in presence of ART was mediated through forming a BLC-ART complex with reduced and shifted electrochemical peak and increased hydrodynamic diameter. ART substantially prevents the temperature-induced reduction in α-helical content with simultaneous increment in other secondary structures like antiparallel, parallel, β-turn and random coils. Nevertheless, the protective role of ART was accepted from the enhanced thermal stability and increased Tm value of BLC in presence of ART at higher temperatures. Our results uncover the mechanism of interaction between ART with BLC and suggest the protective role of ART towards spatiotemporal alteration of BLC by preventing the structural and molecular change in BLC. Thus, the findings advocate ART as a potential therapeutic drug for diseases associated with reduced catalase activity.

Place, publisher, year, edition, pages
Elsevier, 2021
Keywords
Artemisinin, Biophysical study, Bovine liver catalase, Circular dichroism, Enzyme activity, Protein conformation, Thermal melting
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:umu:diva-180539 (URN)10.1016/j.ijbiomac.2021.01.072 (DOI)000619184100042 ()2-s2.0-85100211752 (Scopus ID)
Available from: 2021-02-22 Created: 2021-02-22 Last updated: 2023-09-05Bibliographically approved
Kumari, K., Groza, P. & Aguilo, F. (2021). Regulatory roles of RNA modifications in breast cancer. NAR Cancer, 3(3), Article ID zcab036.
Open this publication in new window or tab >>Regulatory roles of RNA modifications in breast cancer
2021 (English)In: NAR Cancer, E-ISSN 2632-8674, Vol. 3, no 3, article id zcab036Article, review/survey (Refereed) Published
Abstract [en]

Collectively referred to as the epitranscriptome, RNA modifications play important roles in gene expression control regulating relevant cellular processes. In the last few decades, growing numbers of RNA modifications have been identified not only in abundant ribosomal (rRNA) and transfer RNA (tRNA) but also in messenger RNA (mRNA). In addition, many writers, erasers and readers that dynamically regulate the chemical marks have also been characterized. Correct deposition of RNA modifications is prerequisite for cellular homeostasis, and its alteration results in aberrant transcriptional programs that dictate human disease, including breast cancer, the most frequent female malignancy, and the leading cause of cancer-related death in women. In this review, we emphasize the major RNA modifications that are present in tRNA, rRNA and mRNA. We have categorized breast cancer-associated chemical marks and summarize their contribution to breast tumorigenesis. In addition, we describe less abundant tRNA modifications with related pathways implicated in breast cancer. Finally, we discuss current limitations and perspectives on epitranscriptomics for use in therapeutic strategies against breast and other cancers.

Place, publisher, year, edition, pages
Oxford University Press, 2021
National Category
Cancer and Oncology Cell and Molecular Biology
Identifiers
urn:nbn:se:umu:diva-202959 (URN)10.1093/narcan/zcab036 (DOI)000925411800012 ()34541538 (PubMedID)2-s2.0-85126281516 (Scopus ID)
Available from: 2023-01-14 Created: 2023-01-14 Last updated: 2023-09-05Bibliographically approved
Kumari, K., Chainy, G. B. N. & Subudhi, U. (2020). Prospective role of thyroid disorders in monitoring COVID-19 pandemic. Heliyon, 6(12), Article ID e05712.
Open this publication in new window or tab >>Prospective role of thyroid disorders in monitoring COVID-19 pandemic
2020 (English)In: Heliyon, E-ISSN 2405-8440, Vol. 6, no 12, article id e05712Article, review/survey (Refereed) Published
Abstract [en]

COVID-19 pandemic has affected more than 200 countries and 1.3 million individuals have deceased within eleven months. Intense research on COVID-19 occurrence and prevalence enable us to understand that comorbidities play a crucial role in spread and severity of SARS-CoV-2 infection. Chronic kidney disease, diabetes,respiratory diseases and hypertension are among the various morbidities that are prevalent in symptomatic COVID-19 patients. However, the effect of altered thyroid-driven disorders cannot be ignored. Since thyroid hormone critically coordinate and regulate the major metabolism and biochemical pathways, this review is on the potential role of prevailing thyroid disorders in SARS-CoV-2 infection. Direct link of thyroid hormone with several disorders such as diabetes, vitamin D deficiency, obesity, kidney and liver disorders etc. suggests that the prevailing thyroid conditions may affect SARS-CoV-2 infection. Further, we discuss the oxidative stress-induced aging is associated with the degree of SARS-CoV-2 infection. Importantly, ACE2 protein which facilitates the host-cell entry of SARS-CoV-2 using the spike protein, are highly expressed in individuals with abnormal level of thyroid hormone. Altogether, we report that the malfunction of thyroid hormone synthesis may aggravate SARS-CoV-2 infection and thus monitoring the thyroid hormone may help in understanding the pathogenesis of COVID-19.

Place, publisher, year, edition, pages
Elsevier, 2020
Keywords
Thyroid, COVID-19, Hypothyroid, Hyperthyroid, Oxidative stress, Aging, Biochemistry, Endocrinology, Toxicology, Epidemiology, SARS-CoV-2
National Category
Infectious Medicine
Identifiers
urn:nbn:se:umu:diva-179590 (URN)10.1016/j.heliyon.2020.e05712 (DOI)000605645000026 ()33344794 (PubMedID)2-s2.0-85097769814 (Scopus ID)
Available from: 2021-02-04 Created: 2021-02-04 Last updated: 2023-03-23Bibliographically approved
Groza, P., Kumari, K., Destefanis, E., Williams, C., Marchand, V., Motorin, Y., . . . Aguilo, F. Fibrillarin regulates oncogenic protein pools and ribosome protein composition in triple-negative breast cancer.
Open this publication in new window or tab >>Fibrillarin regulates oncogenic protein pools and ribosome protein composition in triple-negative breast cancer
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(English)Manuscript (preprint) (Other academic)
National Category
Cell and Molecular Biology
Identifiers
urn:nbn:se:umu:diva-223012 (URN)
Available from: 2024-04-08 Created: 2024-04-08 Last updated: 2024-04-08
Malla, S., Kumari, K., Martinez Gamero, C., Achour, C., Mermelekas, G., Coege, A., . . . Aguilo, F.LSD1 interacts with CHD7 to regulate the chromatin landscape in mouse embryonic stem cells.
Open this publication in new window or tab >>LSD1 interacts with CHD7 to regulate the chromatin landscape in mouse embryonic stem cells
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(English)Manuscript (preprint) (Other academic)
Abstract [en]

 

 

 

National Category
Cell and Molecular Biology
Research subject
Molecular Biology
Identifiers
urn:nbn:se:umu:diva-206717 (URN)
Available from: 2023-04-14 Created: 2023-04-14 Last updated: 2023-04-14
Malla, S., Kumari, K., Martinez Gamero, C., García-Prieto, c. A., Álvarez-Errico3, D., Stransky, S., . . . Aguilo, F.The catalytic-independent function of LSD1 modulates the epigenetic landscape of mouse embryonic stem cells.
Open this publication in new window or tab >>The catalytic-independent function of LSD1 modulates the epigenetic landscape of mouse embryonic stem cells
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(English)Manuscript (preprint) (Other academic)
Keywords
LSD1, DNA methylation, DNMT1, UHRF1 and USP7
National Category
Cell and Molecular Biology
Research subject
Molecular Biology
Identifiers
urn:nbn:se:umu:diva-206715 (URN)
Available from: 2023-04-14 Created: 2023-04-14 Last updated: 2023-04-14
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