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Title [sv]
Tarmslemhinnans immunologi
Title [en]
Immune defense of the intestinal mucosa
Abstract [sv]
Objective: To understand the mechanisms for how gut adaptive and innate immunity in concert manage to effectuate protection and simultaneously keep immune tolerance to commensals and food. T cells execute regulatory and effector functions in the adaptive immunity while epithelial cells are essential in innate immunity at the epithelial lining where the microbiota and food antigens are encountered. Study areas: - Immunological consequences of bacteria?epithelial cell interactions - Crosstalk between intraepithelial T cells and epithelial cells in immune development and protection and influences thereon from gut microbiota Project design: Functional state of T cells and epithelial cells is analyzed by monitoring expression of genes identified as important in protection or tolerance by comparison of the two cell types purified from small intestinal biopsies with inflamed mucosa due to that the individual was intolerant to dietary gluten, i.e. had celiac disease (CD), and biopsies with non-inflamed mucosa. We isolate and characterize bacteria that are overrepresented in the gut microbiota of CD patients. Gene expression is monitored in small intestine of CD patients and controls, in T84 tight monolayers and in an organoid in vitro model of gut epithelium after challenge with CD-associated bacteria with and without addition of T cell derived effector molecules. Methods: quantitative RT-PCR, two-color immunohistochemistry/morphometry, multicolor FACS, cellsorting, pyrosequensing.
Principal InvestigatorHammarström, Marie-Louise
Coordinating organisation
Umeå University
Funder
Period
2014-01-01 － 2016-12-31
National Category
ImmunologyMicrobiologyImmunology in the medical area
Identifiers
DiVA, id: project:1328Project, id: 2013-04522_VR

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