Umeå University's logo

umu.sePublications
EnglishSvenskaNorsk
Change search
ExportLink to record
Permanent link

Direct link
BETA

Project

Project type/Form of grant
Project grant
Title [sv]
Bakteriella virulens mekanismer hos den opportunistiska patogenen Acinetobacter baumannii och den mångsidiga patogenen Escherichia coli.
Title [en]
Bacterial virulence mechanisms of the opportunistic pathogen Acinetobacter baumannii and the versatile pathogen Escherichia coli.
Abstract [en]
Bacterial virulence mechanisms of the opportunistic pathogen Acinetobacter baumannii and the versatile pathogen Escherichia coli.The purpose is to increase our understanding of the properties and mechanisms by which two types of pathogenic bacteria, the emerging nosocomial pathogen Acinetobacter baumannii and the very versatile extraintestinal pathogenic variants of Escherichia coli (ExPEC), express their virulence-associated properties. We are in particular aiming at specific molecular mechanisms or components that can be targeted as a novel ?Achilles´ heel? of the bacteria. The specific aims are:->To characterize new clinical isolates of A. baumannii with documented disease phenotype.->To establish new genetic recombination technologies allowing for genetic manipulation of the A. baumannii clinical isolates.->To establish relevant in vitro biofilm models and tests for virulence properties in model systems for Gram-negative pathogens.->To characterize biophysical and biomechanical surface properties of A. baumannii clinical isolates.->To investigate the molecular basis for the persistence phenotype of A. baumannii isolates under desiccating and starvation conditions.->To investigate the molecular basis of a novel c-di-GMP phosphodiesterase protein family in regulation of adhesion and biofilm formation.->To design and establish assays for screening of small molecules inhibiting expression of virulence or persistence properties of A. baumannii isolates and pathogenic E. coli.->To screen for small molecules that target A. baumannii infectivity and/or persistence in relevant in vitro model infection systems. The project will be carried out by a research group at Umeå University including one laboratory technician, two postdocs and one PhD student in addition to the project leader. It is planned for a duration of four years and will include several research methods: microbiological, biochemical, biophysical and ultrastructural approaches.There is a need for new approaches to tackle the problem with the emerging and persistent nosocomial pathogens like A. baumannii and we suggest that our multidisciplinary combination of approaches will bring forward new knowledge that can be used for development of new anti-microbial strategies.
Principal InvestigatorUhlin, Bernt Eric
Coordinating organisation
Umeå University
Funder
Period
2016-01-01 － 2019-12-31
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
DiVA, id: project:1484Project, id: 2015-03007_VR
v. 2.46.0
|
WCAG
|
Umeå University Library
|
Register in DiVA
|
Support
|
Search DiVA Portal