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Project type/Form of grant
Title [sv]
Multimodal visualisering och proteomik för att studera flavivirus replikation och molekylära sjukdomsmekanismer
Title [en]
Multimodal imaging and proteomics to study flavivirus replication and molecular disease mechanisms
Abstract [en]
Although flaviviruses cause serious disease in millions of people worldwide, no antiviral treatment is available against them. Our aim is to provide a detailed understanding of molecular disease mechanisms of flaviviruses. We will identify and characterize the architecture and building blocks of virus replication and assembly, to pinpoint key target areas for antiviral drug design. We will use tick-borne encephalitis virus (TBEV) as a model virus. An imaging workflow will be used to allow precise localization of brain structures with active virus replication. These sites will be visualized in 3D at nanometre resolution using cryo-electron tomography. Furthermore, we will identify and characterize new host factors involved in TBEV replication and assembly, with a diverse set of virological model systems developed for TBEV. For detailed studies of the assembly mechanism and architecture of replication complexes we will use a combination of in vitro reconstitution and correlative EM. In this research environment, we combine the unique expertise of four different research groups with specific, complementary and state-of-the-art methodology. This research will take advantage of the technological forefront of ultrastructural analysis using cryo-electron tomography, to reveal in detail how the different components of the virus and cell work together to generate infectious virions. This knowledge will enable the identification of new cellular targets for antiviral therapy.
Principal InvestigatorÖverby Wernstedt, Anna
Coordinating organisation
Umeå University
Funder
Period
2018-12-01 - 2023-12-31
National Category
Cell and Molecular BiologyMicrobiology in the medical area
Identifiers
DiVA, id: project:1561Project, id: 2018-05851_VR

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