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3D optical molecular imaging of the rodent pancreas by OPT and LSFM
Umeå University, Faculty of Medicine, Umeå Centre for Molecular Medicine (UCMM).ORCID iD: 0000-0002-0712-8256
Umeå University, Faculty of Medicine, Umeå Centre for Molecular Medicine (UCMM).ORCID iD: 0000-0001-5923-8572
2023 (English)In: Type-1 diabetes: methods and protocols / [ed] Anna Moore; Ping Wang, New York: Humana Press, 2023, , p. 19p. 1-19Chapter in book (Refereed)
Abstract [en]

The rodent pancreas is the prevalent model system for preclinical diabetes research. However, due to the compound endocrine–exocrine organization of the gland, with the endocrine islets of Langerhans scattered by the thousands throughout the much greater exocrine parenchyma, stereological assessments of endocrine cell mass, commonly insulin-producing ß-cells, are exceedingly challenging. In recent years, optical mesoscopic imaging techniques such as optical projection tomography (OPT) and light sheet fluorescence microscopy (LSFM) have seen dramatic developments, enabling 3D visualization of fluorescently labeled cells in mm- to cm-sized tissues with μm resolution. Here we present a protocol for 3D visualization and “absolute” quantitative assessments of, for example, islet mass throughout the volume of rodent pancreata with maintained spatial context.

Place, publisher, year, edition, pages
New York: Humana Press, 2023. , p. 19p. 1-19
Series
Methods in Molecular Biology, ISSN 1064-3745, E-ISSN 1940-6029 ; 2592
Keywords [en]
Diabetes, Insulin, Islets of Langerhans, Light sheet fluorescence microscopy, Optical clearing, Optical projection tomography, Pancreas, Rodents, Whole mount immunohistochemistry
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-202060DOI: 10.1007/978-1-0716-2807-2_1PubMedID: 36507982Scopus ID: 2-s2.0-85144475952ISBN: 978-1-0716-2806-5 (print)ISBN: 978-1-0716-2809-6 (print)ISBN: 978-1-0716-2807-2 (electronic)OAI: oai:DiVA.org:umu-202060DiVA, id: diva2:1723620
Available from: 2023-01-03 Created: 2023-01-03 Last updated: 2023-03-23Bibliographically approved

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Hahn, MaxAhlgren, Ulf

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Total: 249 hits
CiteExportLink to record
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