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Contribution of major FLM isoforms to temperature-dependent flowering in Arabidopsis thaliana
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysiologisk botanik. Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Umeå Plant Science Centre (UPSC). Max Planck Institute for Developmental Biology, Department of Molecular Biology, Spemannstr. 35, 72076 Tübingen, Germany.ORCID-id: 0000-0002-0068-2967
2017 (engelsk)Inngår i: Journal of Experimental Botany, ISSN 0022-0957, E-ISSN 1460-2431, Vol. 68, nr 18, s. 5117-5127Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

FLOWERING LOCUS M (FLM), a component of the thermosensory flowering time pathway in Arabidopsis thaliana, is regulated by temperature-dependent alternative splicing (AS). The main splicing variant, FLM-beta, is a well-documented floral repressor that is down-regulated in response to increasing ambient growth temperature. Two hypotheses have been formulated to explain how flowering time is modulated by AS of FLM. In the first model a second splice variant, FLM-delta, acts as a dominant negative isoform that competes with FLM-beta at elevated ambient temperatures, thereby indirectly promoting flowering. Alternatively, it has been suggested that the induction of flowering at elevated temperatures is caused only by reduced FLM-beta expression. To better understand the role of the two FLM splice forms, we employed CRISPR/Cas9 technology to specifically delete the exons that characterize each splice variant. Lines that produced repressive FLM-beta but were incapable of producing FLM-delta were late flowering. In contrast, FLM-beta knockout lines that still produced FLM-delta flowered early, but not earlier than the flm-3 loss of function mutant, as would be expected if FLM-delta had a dominant-negative effect on flowering. Our data support the role of FLM-beta as a flower repressor and provide evidence that a contribution of FLM-delta to the regulation of flowering time in wild-type A. thaliana seems unlikely.

sted, utgiver, år, opplag, sider
Oxford University Press, 2017. Vol. 68, nr 18, s. 5117-5127
Emneord [en]
Arabidopsis thaliana, CRISPR/Cas9, FLOWERING LOCUS M (FLM), flowering time, splice isoforms, temperature-dependent alternative splicing
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Identifikatorer
URN: urn:nbn:se:umu:diva-142004DOI: 10.1093/jxb/erx328ISI: 000414371700011PubMedID: 29036339Scopus ID: 2-s2.0-85034604580OAI: oai:DiVA.org:umu-142004DiVA, id: diva2:1158935
Tilgjengelig fra: 2017-11-21 Laget: 2017-11-21 Sist oppdatert: 2024-07-02bibliografisk kontrollert

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