Umeå University's logo

umu.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Transcriptome wide identification and characterization of NO-responsive WRKY transcription factors in Arabidopsis thaliana L.
Laboratory of Plant Functional Genomics School of Applied Biosciences, Kyungpook National University, Daegu, Republic of Korea.ORCID-id: 0000-0002-7072-451x
Vise andre og tillknytning
2018 (engelsk)Inngår i: Environmental and Experimental Botany, ISSN 0098-8472, E-ISSN 1873-7307, Vol. 148, s. 128-143Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

WRKY transcription factors are important plant-specific regulatory genes characterized by one or two conserved WRKY domain(s) usually followed by a zinc-finger motif. In this study using Arabidopsis thaliana, the RNA-Seq based transcriptomic analysis showed differential expression of 33 genes encoding WRKY TFs in response to the nitric oxide (NO) donor S-Nitrosocysteine (CySNO). Interestingly, 93.9% of these TFs were up-regulated with at least 2-fold change, suggesting their putative involvement in NO mediated gene regulation. GO- analysis of all the 33 transcriptomic elements showed their putative involvement in biological processes such as abiotic stress tolerance and defense against fungal pathogens (89.39 fold enrichment). Analysis of the NO-responsive AtWRKY TFs promoter region revealed the presence of the cis-acting elements such as ABRE, EIRE, ERE, and MBS involved in osmotic stress response, maximal elicitor-mediated activation, and drought-stress regulation. The analysis of NO-responsive AtWRKY TF motifs and their comparison with rice, soybean, and tomato orthologs suggested that members of the WRKY family belonging to the same group shared similar motifs and phylogenetic tree suggested that these TFs were highly conserved. Validation of transcriptomic data through quantitative real time-PCR showed a high correlation coefficient (0.85) indicating the high reliability and similarity of both types of analysis. Comparison of the NO-responsive and non-responsive WRKYs showed the presence of tyrosine (T) and cysteine (C) residues at a distance of 7 residues from the WRKYGQK motif which may serve as potential targets for modification by NO via tyrosine nitration and S-nitrosylation. We also validated the response of WRKYs through in vivo analysis using atwrky62 loss of function mutant and the results indicated a negative role of AtWRKY62 in plant growth. Furthermore, atwrky62 showed significantly less SNO contents compared to wild type plants indicating putative role of AtWRKY62 in NO metabolism.

sted, utgiver, år, opplag, sider
Elsevier, 2018. Vol. 148, s. 128-143
Emneord [en]
Arabidopsis thaliana, Gene ontology, Nitrosative stress, Promoter analysis, qRT-PCR, RNA-Seq, WRKY
HSV kategori
Identifikatorer
URN: urn:nbn:se:umu:diva-176700DOI: 10.1016/j.envexpbot.2018.01.010ISI: 000426224700012OAI: oai:DiVA.org:umu-176700DiVA, id: diva2:1500951
Tilgjengelig fra: 2020-11-14 Laget: 2020-11-14 Sist oppdatert: 2020-11-16bibliografisk kontrollert

Open Access i DiVA

fulltext(2938 kB)182 nedlastinger
Filinformasjon
Fil FULLTEXT01.pdfFilstørrelse 2938 kBChecksum SHA-512
9abbb043a2954d1250693ba7381365bacd0db3985ed165d4b1c731b5191e5259b20256c5399814f24c44258a540fe915a1c26c5fe65c7f303b95195c9a4bc18a
Type fulltextMimetype application/pdf

Andre lenker

Forlagets fulltekst

Person

Imran, Qari Muhammad

Søk i DiVA

Av forfatter/redaktør
Imran, Qari Muhammad
I samme tidsskrift
Environmental and Experimental Botany

Søk utenfor DiVA

GoogleGoogle Scholar
Totalt: 182 nedlastinger
Antall nedlastinger er summen av alle nedlastinger av alle fulltekster. Det kan for eksempel være tidligere versjoner som er ikke lenger tilgjengelige

doi
urn-nbn

Altmetric

doi
urn-nbn
Totalt: 239 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf