Umeå University's logo

umu.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Nitric oxide mediated transcriptome profiling reveals activation of multiple regulatory pathways in Arabidopsis thaliana
Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu, South Kore.ORCID-id: 0000-0002-7072-451x
Vise andre og tillknytning
2016 (engelsk)Inngår i: Frontiers in Plant Science, E-ISSN 1664-462X, Vol. 7Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Imbalance between the accumulation and removal of nitric oxide and its derivatives is a challenge faced by all plants at the cellular level, and is especially important under stress conditions. Exposure of plants to various biotic and abiotic stresses causes rapid changes in cellular redox tone potentiated by the rise in reactive nitrogen species that serve as signaling molecules in mediating defensive responses. To understand mechanisms mediated by these signaling molecules, we performed a large-scale analysis of the Arabidopsis transcriptome induced by nitrosative stress. We generated an average of 84 and 91 million reads from three replicates each of control and 1 mM S-nitrosocysteine (CysNO)-infiltrated Arabidopsis leaf samples, respectively. After alignment, more than 95% of all reads successfully mapped to the reference and 32,535 genes and 55,682 transcripts were obtained. CysNO infiltration caused differential expression of 6436 genes (3448 up-regulated and 2988 down-regulated) and 6214 transcripts (3335 up-regulated and 2879 down-regulated) 6 h post-infiltration. These differentially expressed genes were found to be involved in key physiological processes, including plant defense against various biotic and abiotic stresses, hormone signaling, and other developmental processes. After quantile normalization of the FPKM values followed by student's T-test (P < 0.05) we identified 1165 DEGs (463 up-regulated and 702 down-regulated) with at least 2-folds change in expression after CysNO treatment. Expression patterns of selected genes involved in various biological pathways were verified using quantitative real-time PCR. This study provides comprehensive information about plant responses to nitrosative stress at transcript level and would prove helpful in understanding and incorporating mechanisms associated with nitrosative stress responses in plants.

sted, utgiver, år, opplag, sider
Frontiers Media S.A., 2016. Vol. 7
Emneord [en]
Arabidopsis thaliana transcriptomic analysis, nitrosative stress, RNA-Seq profiling, RNS signaling
HSV kategori
Identifikatorer
URN: urn:nbn:se:umu:diva-176691DOI: 10.3389/fpls.2016.00975ISI: 000378696400002PubMedID: 27446194OAI: oai:DiVA.org:umu-176691DiVA, id: diva2:1500960
Tilgjengelig fra: 2020-11-14 Laget: 2020-11-14 Sist oppdatert: 2024-01-17bibliografisk kontrollert

Open Access i DiVA

fulltext(4937 kB)105 nedlastinger
Filinformasjon
Fil FULLTEXT01.pdfFilstørrelse 4937 kBChecksum SHA-512
86dc3cf3d1c7b7514b5fac80dc0f4c799c21af7564d417ce98eb2dcab8a08482c5205b917b487088ec1083881f2fa925f76cfcd1387762f2ea1f7776486f7411
Type fulltextMimetype application/pdf

Andre lenker

Forlagets fulltekstPubMed

Person

Imran, Qari Muhammad

Søk i DiVA

Av forfatter/redaktør
Imran, Qari Muhammad
I samme tidsskrift
Frontiers in Plant Science

Søk utenfor DiVA

GoogleGoogle Scholar
Totalt: 105 nedlastinger
Antall nedlastinger er summen av alle nedlastinger av alle fulltekster. Det kan for eksempel være tidligere versjoner som er ikke lenger tilgjengelige

doi
pubmed
urn-nbn

Altmetric

doi
pubmed
urn-nbn
Totalt: 185 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf