Umeå University's logo

umu.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
3D optical molecular imaging of the rodent pancreas by OPT and LSFM
Umeå universitet, Medicinska fakulteten, Umeå centrum för molekylär medicin (UCMM).ORCID-id: 0000-0002-0712-8256
Umeå universitet, Medicinska fakulteten, Umeå centrum för molekylär medicin (UCMM).ORCID-id: 0000-0001-5923-8572
2023 (engelsk)Inngår i: Type-1 diabetes: methods and protocols / [ed] Anna Moore; Ping Wang, New York: Humana Press, 2023, , s. 19s. 1-19Kapittel i bok, del av antologi (Fagfellevurdert)
Abstract [en]

The rodent pancreas is the prevalent model system for preclinical diabetes research. However, due to the compound endocrine–exocrine organization of the gland, with the endocrine islets of Langerhans scattered by the thousands throughout the much greater exocrine parenchyma, stereological assessments of endocrine cell mass, commonly insulin-producing ß-cells, are exceedingly challenging. In recent years, optical mesoscopic imaging techniques such as optical projection tomography (OPT) and light sheet fluorescence microscopy (LSFM) have seen dramatic developments, enabling 3D visualization of fluorescently labeled cells in mm- to cm-sized tissues with μm resolution. Here we present a protocol for 3D visualization and “absolute” quantitative assessments of, for example, islet mass throughout the volume of rodent pancreata with maintained spatial context.

sted, utgiver, år, opplag, sider
New York: Humana Press, 2023. , s. 19s. 1-19
Serie
Methods in Molecular Biology, ISSN 1064-3745, E-ISSN 1940-6029 ; 2592
Emneord [en]
Diabetes, Insulin, Islets of Langerhans, Light sheet fluorescence microscopy, Optical clearing, Optical projection tomography, Pancreas, Rodents, Whole mount immunohistochemistry
HSV kategori
Identifikatorer
URN: urn:nbn:se:umu:diva-202060DOI: 10.1007/978-1-0716-2807-2_1PubMedID: 36507982Scopus ID: 2-s2.0-85144475952ISBN: 978-1-0716-2806-5 (tryckt)ISBN: 978-1-0716-2809-6 (tryckt)ISBN: 978-1-0716-2807-2 (digital)OAI: oai:DiVA.org:umu-202060DiVA, id: diva2:1723620
Tilgjengelig fra: 2023-01-03 Laget: 2023-01-03 Sist oppdatert: 2023-03-23bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fulltekstPubMedScopus

Person

Hahn, MaxAhlgren, Ulf

Søk i DiVA

Av forfatter/redaktør
Hahn, MaxAhlgren, Ulf
Av organisasjonen

Søk utenfor DiVA

GoogleGoogle Scholar

doi
pubmed
isbn
urn-nbn

Altmetric

doi
pubmed
isbn
urn-nbn
Totalt: 249 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf