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As the development oftechnical aids for telework has progressed, work has become moreflexible in time and space. Among academics, the opportunity to telework has beenembraced by most, but it is unclear how it relates to their health and well-being. The aimof this study was to determine how frequency and amount of telework is associated withperceived health, stress, recuperation, work-life balance, and intrinsic work motivationamong teaching and research academics. An electronic questionnaire was sent to juniorlecturers, senior lecturers, and professors at Swedish public universities. It included theGeneral Health Questionnaire, Work Stress Questionnaire, items for assessing recupera-tion, the Basic Psychological Need Satisfaction at Work scale, and parts of CopenhagenPsychosocial Questionnaire, as well as questions about the frequency and amount oftelework performed. In total, 392 academics responded to the survey. Multivariateanalysis of variance showed significant differences between groups of academics withdifferent telework frequency (p < 0.05). Univariate analyses of variance showed thatratings of stress related to indistinct organization and conflicts were higher amongacademics that telework several times per week or more than among academics thattelework less than once per month. In regression analyses ofassociations between amountof telework (in hours per week) and the dependent variables, no significant effectswere found. Although it cannot be concluded whether stress is a cause or an effectof frequent telework, the findings warrant further attention to academics whotelework frequently.