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Comprehensive Analyses of Nitric Oxide-Induced Plant Stem Cell-Related Genes in Arabidopsis thaliana
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2019 (Engelska)Ingår i: Genes, ISSN 2073-4425, E-ISSN 2073-4425, Vol. 10, nr 3, artikel-id 190Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Plant stem cells are pluripotent cells that have diverse applications in regenerative biology and medicine. However, their roles in plant growth and disease resistance are often overlooked. Using high-throughput RNA-seq data, we identified approximately 20 stem cell-related differentially expressed genes (DEGs) that were responsive to the nitric oxide (NO) donor S-nitrosocysteine (CySNO) after six hours of infiltration. Among these DEGs, the highest number of positive correlations (R ≥ 0.8) was observed for CLAVATA3/EMBRYO SURROUNDING REGION-RELATED (CLE) 12. Gene ontology (GO) terms for molecular function showed DEGs associated with signal transduction and receptor activity. A promoter study of these DEGs showed the presence of cis-acting elements that are involved in growth as well as the regulation of abiotic and biotic stress. Phylogenetic analysis of the Arabidopsis stem cell-related genes and their common orthologs in rice, soybean, poplar, and tomato suggested that most soybean stem cell-related genes were grouped with the Arabidopsis CLE type of stem cell genes, while the rice stem cell-related genes were grouped with the Arabidopsis receptor-like proteins. The functional genomic-based characterization of the role of stem cell DEGs showed that under control conditions, the clv1 mutant showed a similar phenotype to that of the wild-type (WT) plants; however, under CySNO-mediated nitrosative stress, clv1 showed increased shoot and root length compared to WT. Furthermore, the inoculation of clv1 with virulent Pst DC3000 showed a resistant phenotype with fewer pathogens growing at early time points. The qRT-PCR validation and correlation with the RNA-seq data showed a Pearson correlation coefficient of >0.8, indicating the significantly high reliability of the RNA-seq analysis.

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MDPI, 2019. Vol. 10, nr 3, artikel-id 190
Nyckelord [en]
Arabidopsis thaliana, stem cells, basal defense, promoter analysis, gene ontology
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Växtbioteknologi
Identifikatorer
URN: urn:nbn:se:umu:diva-176709DOI: 10.3390/genes10030190ISI: 000464471300008OAI: oai:DiVA.org:umu-176709DiVA, id: diva2:1500971
Tillgänglig från: 2020-11-14 Skapad: 2020-11-14 Senast uppdaterad: 2020-11-18Bibliografiskt granskad

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Imran, Qari Muhammad

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Totalt: 131 träffar
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