Amperometric biosensor for formic acid in airShow others and affiliations
2000 (English)In: Sensors and actuators. B, Chemical, ISSN 0925-4005, E-ISSN 1873-3077, Vol. 70, no 1–3, p. 182-187Article in journal (Refereed) Published
Abstract [en]
The possibility of developing a simple, inexpensive and specific personal passive “real-time” air sampler incorporating a biosensor for formic acid was investigated. The sensor is based on the enzymatic reaction between formic acid and formate dehydrogenase (FDH) with nicotinamide adenine dinucleotide (NAD+) as a co-factor and Meldola's blue as mediator. An effective way to immobilise the enzyme, co-factor and Meldola's blue on screen-printed, disposable, electrodes was found to be in a mixture of glycerol and phosphate buffer covered with a gas-permeable membrane. Steady-state current was reached after 4–15 min and the limit of detection was calculated to be below 1 mg/m3. However, the response decreased by 50% after storage at −15°C for 1 day.
Place, publisher, year, edition, pages
Elsevier, 2000. Vol. 70, no 1–3, p. 182-187
Keywords [en]
Biosensor, Air monitoring, Formic acid, Gas-permeable membrane, Glycerol
National Category
Other Environmental Biotechnology
Identifiers
URN: urn:nbn:se:umu:diva-149303DOI: 10.1016/S0925-4005(00)00566-9ISI: 000090126600026Scopus ID: 2-s2.0-0034324745OAI: oai:DiVA.org:umu-149303DiVA, id: diva2:1220769
2011-02-042018-06-192023-03-23Bibliographically approved