Umeå University's logo

umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Analysis of transcription factors among differentially expressed genes induced by drought stress in Populus davidiana
Show others and affiliations
2017 (English)In: 3 Biotech, E-ISSN 2190-5738, Vol. 7, article id 209Article in journal (Refereed) Published
Abstract [en]

Populus davidiana is native to the Korean Peninsula and is one of the most dominant and abundantly growing forest trees in eastern Asia. Compared to other Populus species such as P. trichocarpa, P. euphratica, and P. tremula, relatively little is known about P. davidiana. Here, we performed transcriptomic analysis of P. davidiana under drought stress induced by 10% polyethylene glycol. A total of 12,403 and 12,414 differentially expressed genes (DEGs) were successfully annotated with the P. trichocarpa reference genome after 6 and 12 h of treatment, respectively. Of these, a total of 404 genes (238 up-regulated and 166 down-regulated) after 6 h and 359 genes (187 up-regulated and 172 down-regulated) after 12 h of treatment were identified as transcription factors. Transcription factors known to be key genes for drought stress response, such as AP2-EREB, WRKY, C2H2, and NAC, were identified. This results suggesting that early induction of these genes affected initiation of transcriptional regulation in response to drought stress. Quantitative real-time PCR results of selected genes showed highly significant (R = 0.93) correlation with RNA-Seq data. Interestingly, the expression pattern of some transcription factors was P. davidiana specific. The sequence of P. davidiana ortholog of P. trichocarpa gene POPTR_0018s10230, which plays an important role in plant response to drought, was further analyzed as our RNA-Seq results showed highly significant changes in the expression of this gene following the stress treatment. Sequence of the gene was compared to P. trichocarpa gene sequence using cloning-based sequencing. Additionally, we generated a predicted 3D protein structure for the gene product. Results indicated that the amino acid sequence of P. davidiana-specific POPTR_0018s10230 is different at six different positions compared to P. trichocarpa, resulting in a significantly different structure of the protein. Identifying the transcription factors expressed in P. davidiana under drought stress will not only offer clues for understanding the underlying mechanisms involved in drought stress physiology but also serve as a basis for future molecular studies on this species.

Place, publisher, year, edition, pages
Springer, 2017. Vol. 7, article id 209
Keywords [en]
Drought stress, Transcriptome, Poplar, Transcription factors
National Category
Plant Biotechnology
Identifiers
URN: urn:nbn:se:umu:diva-176695DOI: 10.1007/s13205-017-0858-7ISI: 000404680500015PubMedID: 28667649OAI: oai:DiVA.org:umu-176695DiVA, id: diva2:1500953
Available from: 2020-11-14 Created: 2020-11-14 Last updated: 2023-11-14Bibliographically approved

Open Access in DiVA

fulltext(2404 kB)125 downloads
File information
File name FULLTEXT01.pdfFile size 2404 kBChecksum SHA-512
e0006e369d208f3e9069778cd05dee057f1c2b9ee1339c29f4c38088386d7fd9082cacd3671b7b2055ba66e83a0e96f76fc4ca0898efff9a7f2adc66e01742ad
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records

Imran, Qari Muhammad

Search in DiVA

By author/editor
Imran, Qari Muhammad
In the same journal
3 Biotech
Plant Biotechnology

Search outside of DiVA

GoogleGoogle Scholar
Total: 125 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 227 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf