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Dysregulated microRNA expression in rheumatoid arthritis families-a comparison between rheumatoid arthritis patients, their first-degree relatives, and healthy controls
Umeå University, Faculty of Medicine, Department of Clinical Microbiology. (Kristina Lejon)ORCID iD: 0000-0002-2051-2973
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Rheumatology.
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Rheumatology.
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Rheumatology.ORCID iD: 0000-0001-8259-3863
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2021 (English)In: Clinical Rheumatology, ISSN 0770-3198, E-ISSN 1434-9949, Vol. 40, no 6, p. 2387-2394Article in journal (Refereed) Published
Abstract [en]

OBJECTIVE: Recent studies have demonstrated an altered expression of certain microRNAs in patients with rheumatoid arthritis (RA) as well as their first-degree relatives (FDRs) compared to healthy controls (HCs), suggesting a role of microRNA in the progression of the disease. To corroborate this, a set of well-characterized RA families originating from northern Sweden were analyzed for differential expression of a selected set of microRNAs.

METHOD: MicroRNA was isolated from frozen peripheral blood cells obtained from 21 different families and included 26 RA patients, 22 FDRs, and 21 HCs. Expression of the selected microRNAs miR-22-3p, miR-26b-5p, miR-34a-3p, miR-103a-3p, miR-142-3p, miR-146a-5p, miR-155, miR-346, and miR-451a was determined by a two-step quantitative real-time polymerase chain reaction (qRT-PCR). Statistical analysis including clinical variables was applied.

RESULTS: Out of the nine selected microRNAs that previously have been linked to RA, we confirmed four after adjusting for age and gender, i.e., miR-22-3p (p = 0.020), miR-26b-5p (p = 0.018), miR-142-3p (p = 0.005), and miR-155 (p = 0.033). Moreover, a significant trend with an intermediate microRNA expression in FDR was observed for the same four microRNAs. In addition, analysis of the effect of corticosteroid use showed modulation of miR-103a-3p expression.

CONCLUSIONS: We confirm that microRNAs seem to be involved in the development of RA, and that the expression pattern in FDR is partly overlapping with RA patients. The contribution of single microRNAs in relation to the complex network including all microRNAs and other molecules is still to be revealed. Key Points • Expression levels of miR-22-3p, miR-26b-5p, miR-142-3p, and miR-155 were significantly altered in RA patients compared to those in controls. • In first-degree relatives, a significant trend with an intermediate microRNA expression in FDR was observed for the same four microRNAs.

Place, publisher, year, edition, pages
Springer, 2021. Vol. 40, no 6, p. 2387-2394
Keywords [en]
Autoimmunity, MicroRNA family, Rheumatoid arthritis, Sweden
National Category
Rheumatology and Autoimmunity
Identifiers
URN: urn:nbn:se:umu:diva-176964DOI: 10.1007/s10067-020-05502-9ISI: 000591447600001PubMedID: 33210166Scopus ID: 2-s2.0-85096216498OAI: oai:DiVA.org:umu-176964DiVA, id: diva2:1502927
Funder
Swedish Research Council, 201802551King Gustaf V Jubilee FundSwedish Rheumatism AssociationAvailable from: 2020-11-23 Created: 2020-11-23 Last updated: 2023-03-24Bibliographically approved

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Renman, EmmaBrink, MikaelÄrlestig, LisbethRantapää-Dahlqvist, SolbrittLejon, Kristina

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