Umeå University's logo

umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Overexpression of vesicle-associated membrane protein PttVAP27-17 as a tool to improve biomass production and the overall saccharification yields in Populus trees
Umeå University, Faculty of Science and Technology, Department of Chemistry.ORCID iD: 0000-0003-2798-6298
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).ORCID iD: 0000-0003-2673-9113
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC). Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Sweden.ORCID iD: 0000-0001-7049-6978
Umeå University, Faculty of Science and Technology, Department of Chemistry.ORCID iD: 0000-0001-8445-0559
Show others and affiliations
2021 (English)In: Biotechnology for Biofuels, ISSN 1754-6834, E-ISSN 1754-6834, Vol. 14, no 1, article id 43Article in journal (Refereed) Published
Abstract [en]

Background: Bioconversion of wood into bioproducts and biofuels is hindered by the recalcitrance of woody raw material to bioprocesses such as enzymatic saccharification. Targeted modification of the chemical composition of the feedstock can improve saccharification but this gain is often abrogated by concomitant reduction in tree growth.

Results: In this study, we report on transgenic hybrid aspen (Populus tremula × tremuloides) lines that showed potential to increase biomass production both in the greenhouse and after 5 years of growth in the field. The transgenic lines carried an overexpression construct for Populus tremula × tremuloides vesicle-associated membrane protein (VAMP)-associated protein PttVAP27-17 that was selected from a gene-mining program for novel regulators of wood formation. Analytical-scale enzymatic saccharification without any pretreatment revealed for all greenhouse-grown transgenic lines, compared to the wild type, a 20–44% increase in the glucose yield per dry weight after enzymatic saccharification, even though it was statistically significant only for one line. The glucose yield after enzymatic saccharification with a prior hydrothermal pretreatment step with sulfuric acid was not increased in the greenhouse-grown transgenic trees on a dry-weight basis, but increased by 26–50% when calculated on a whole biomass basis in comparison to the wild-type control. Tendencies to increased glucose yields by up to 24% were present on a whole tree biomass basis after acidic pretreatment and enzymatic saccharification also in the transgenic trees grown for 5 years on the field when compared to the wild-type control.

Conclusions: The results demonstrate the usefulness of gene-mining programs to identify novel genes with the potential to improve biofuel production in tree biotechnology programs. Furthermore, multi-omic analyses, including transcriptomic, proteomic and metabolomic analyses, performed here provide a toolbox for future studies on the function of VAP27 proteins in plants.

Place, publisher, year, edition, pages
BioMed Central, 2021. Vol. 14, no 1, article id 43
Keywords [en]
Bioprocessing, Growth, Metabolomics, Populus, Proteomics, Transcriptomics, VAMP, VAMP-associated protein, VAP27, Vesicle-associated membrane protein
National Category
Plant Biotechnology
Identifiers
URN: urn:nbn:se:umu:diva-180984DOI: 10.1186/s13068-021-01895-0ISI: 000620931600002Scopus ID: 2-s2.0-85100873005OAI: oai:DiVA.org:umu-180984DiVA, id: diva2:1534200
Available from: 2021-03-05 Created: 2021-03-05 Last updated: 2023-11-06Bibliographically approved

Open Access in DiVA

fulltext(1716 kB)169 downloads
File information
File name FULLTEXT01.pdfFile size 1716 kBChecksum SHA-512
8c9efb19090b31561adb7b18ffae5835b52450ff47ccc25af8de8139332cfa4324e45efb468a788aad0f2dc3b1ea45aab8c174a62b7fbd84d1a25aeb45212677
Type fulltextMimetype application/pdf

Other links

Publisher's full textScopus

Authority records

Gandla, Madhavi LathaMähler, NiklasEscamez, SachaSkotare, TomasBygdell, JoakimHvidsten, Torgeir R.Trygg, JohanTuominen, HanneleJönsson, Leif J.

Search in DiVA

By author/editor
Gandla, Madhavi LathaMähler, NiklasEscamez, SachaSkotare, TomasBygdell, JoakimHvidsten, Torgeir R.Trygg, JohanTuominen, HanneleJönsson, Leif J.
By organisation
Department of ChemistryDepartment of Plant PhysiologyUmeå Plant Science Centre (UPSC)
In the same journal
Biotechnology for Biofuels
Plant Biotechnology

Search outside of DiVA

GoogleGoogle Scholar
Total: 169 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 389 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf