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Mesoscopic Optical Imaging of the Pancreas: Revisiting Pancreatic Anatomy and Pathophysiology
Umeå University, Faculty of Medicine, Umeå Centre for Molecular Medicine (UCMM).
Umeå University, Faculty of Medicine, Umeå Centre for Molecular Medicine (UCMM).ORCID iD: 0000-0002-0712-8256
Umeå University, Faculty of Medicine, Umeå Centre for Molecular Medicine (UCMM).ORCID iD: 0000-0003-3445-7829
Umeå University, Faculty of Medicine, Umeå Centre for Molecular Medicine (UCMM).ORCID iD: 0000-0001-5923-8572
2021 (English)In: Frontiers in Endocrinology, E-ISSN 1664-2392, Vol. 12, article id 633063Article, review/survey (Refereed) Published
Abstract [en]

The exocrine-endocrine multipart organization of the pancreas makes it an exceedingly challenging organ to analyze, quantitatively and spatially. Both in rodents and humans, estimates of the pancreatic cellular composition, including beta-cell mass, has been largely relying on the extrapolation of 2D stereological data originating from limited sample volumes. Alternatively, they have been obtained by low resolution non-invasive imaging techniques providing little detail regarding the anatomical organization of the pancreas and its cellular and/or molecular make up. In this mini-review, the state of the art and the future potential of currently existing and emerging high-resolution optical imaging techniques working in the mm-cm range with μm resolution, here referred to as mesoscopic imaging approaches, will be discussed regarding their contribution toward a better understanding of pancreatic anatomy both in normal conditions and in the diabetic setting. In particular, optical projection tomography (OPT) and light sheet fluorescence microscopy (LSFM) imaging of the pancreas and their associated tissue processing and computational analysis protocols will be discussed in the light of their current capabilities and future potential to obtain more detailed 3D-spatial, quantitative, and molecular information of the pancreas.

Place, publisher, year, edition, pages
Frontiers Media S.A., 2021. Vol. 12, article id 633063
Keywords [en]
diabetes, light sheet fluorescence microscopy, mesoscopic imaging, optical projection tomography, pancreas
National Category
Endocrinology and Diabetes
Identifiers
URN: urn:nbn:se:umu:diva-182014DOI: 10.3389/fendo.2021.633063ISI: 000629989000001Scopus ID: 2-s2.0-85102893401OAI: oai:DiVA.org:umu-182014DiVA, id: diva2:1542008
Available from: 2021-04-06 Created: 2021-04-06 Last updated: 2024-01-17Bibliographically approved

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Alanentalo, TomasHahn, MaxWillekens, Stefanie M. A.Ahlgren, Ulf

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