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Neutron reflectometry and NMR spectroscopy of full-length Bcl-2 protein reveal its membrane localization and conformation
Umeå University, Faculty of Science and Technology, Department of Chemistry.ORCID iD: 0000-0002-5636-2567
Umeå University, Faculty of Science and Technology, Department of Chemistry.ORCID iD: 0000-0002-4480-1219
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2021 (English)In: Communications Biology, E-ISSN 2399-3642, Vol. 4, no 1, article id 507Article in journal (Refereed) Published
Abstract [en]

B-cell lymphoma 2 (Bcl-2) proteins are the main regulators of mitochondrial apoptosis. Anti-apoptotic Bcl-2 proteins possess a hydrophobic tail-anchor enabling them to translocate to their target membrane and to shift into an active conformation where they inhibit pro-apoptotic Bcl-2 proteins to ensure cell survival. To address the unknown molecular basis of their cell-protecting functionality, we used intact human Bcl-2 protein natively residing at the mitochondrial outer membrane and applied neutron reflectometry and NMR spectroscopy. Here we show that the active full-length protein is entirely buried into its target membrane except for the regulatory flexible loop domain (FLD), which stretches into the aqueous exterior. The membrane location of Bcl-2 and its conformational state seems to be important for its cell-protecting activity, often infamously upregulated in cancers. Most likely, this situation enables the Bcl-2 protein to sequester pro-apoptotic Bcl-2 proteins at the membrane level while sensing cytosolic regulative signals via its FLD region.

Place, publisher, year, edition, pages
Springer Nature, 2021. Vol. 4, no 1, article id 507
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-182710DOI: 10.1038/s42003-021-02032-1ISI: 000647062000003Scopus ID: 2-s2.0-85105112344OAI: oai:DiVA.org:umu-182710DiVA, id: diva2:1549088
Funder
Swedish Research CouncilSwedish Cancer SocietyThe Kempe FoundationsKnut and Alice Wallenberg FoundationAvailable from: 2021-05-04 Created: 2021-05-04 Last updated: 2023-09-05Bibliographically approved

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Ul Mushtaq, AmeeqÅdén, JörgenDingeldein, Artur P. G.Sparrman, TobiasGröbner, Gerhard

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