Umeå universitets logga

umu.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Droplet digital PCR applied to environmental DNA, a promising method to estimate fish population abundance from humic-rich aquatic ecosystems
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för ekologi, miljö och geovetenskap. Molecular Ecology Group, Department of Wildlife, Fish and Environmental Studies, SLU, Umeå, Sweden.ORCID-id: 0000-0001-9143-7061
Molecular Ecology Group, Department of Wildlife, Fish and Environmental Studies, SLU, Umeå, Sweden; Fisheries, Wildlife and Conservation Biology Program, Department of Forestry and Environmental Resources, North Carolina State University, NC, Raleigh, United States.
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för ekologi, miljö och geovetenskap.
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för ekologi, miljö och geovetenskap.
Visa övriga samt affilieringar
2021 (Engelska)Ingår i: Environmental DNA, E-ISSN 2637-4943, Vol. 3, nr 2, s. 343-352Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Measures of environmental DNA (eDNA) concentrations in water samples have the potential to be both a cost-efficient and a nondestructive method to estimate fish population abundance. However, the inherent temporal and spatial variability in abiotic and biotic conditions in aquatic systems have been suggested to be a major obstacle to determine relationships between fish eDNA concentrations and fish population abundance. Moreover, once water samples are collected, methodological biases are common, which introduces additional sources of variation to potential relationships between eDNA concentrations and fish population abundance. Here, we evaluate the performance of applying the droplet digital PCR (ddPCR) method to estimate fish population abundance in experimental enclosures. Using large-scale enclosure ecosystems that contain populations of nine-spined stickleback (Pungitius pungitius), we compared the concentrations of fish eDNA (COI mitochondrial region, 134 bp) obtained with the ddPCR method with high precision estimates of fish population abundance (i.e., number of individuals) and biomass. To evaluate the effects of contrasted concentrations of humic substances (potential PCR inhibitors) on the performance of ddPCR assays, we manipulated natural dissolved organic carbon (DOC) concentrations (range 4–11 mg/L) in the enclosures. Additionally, water temperature (+2°C) was manipulated in half of the enclosures. Results showed positive relationships between eDNA concentration and fish abundance and biomass estimates although unexplained variation remained. Still and importantly, fish eDNA estimates from high DOC enclosures were not lowered by potential inhibitory effects with our procedure. Finally, water temperature (although only 2°C difference) was neither detected as a significant factor influencing fish eDNA estimates. Altogether, our work highlights that ddPCR-based eDNA is a promising method for future quantification of fish population abundance in natural systems.

Ort, förlag, år, upplaga, sidor
John Wiley & Sons, 2021. Vol. 3, nr 2, s. 343-352
Nyckelord [en]
ddPCR, environmental DNA, fish population estimates, nine-spined sticklebacks, species-specific detection
Nationell ämneskategori
Evolutionsbiologi
Identifikatorer
URN: urn:nbn:se:umu:diva-183699DOI: 10.1002/edn3.115Scopus ID: 2-s2.0-85106016581OAI: oai:DiVA.org:umu-183699DiVA, id: diva2:1558566
Forskningsfinansiär
Forskningsrådet Formas, CTS16:84Forskningsrådet Formas, CTS18:812Knut och Alice Wallenbergs Stiftelse, 2016.0083Tillgänglig från: 2021-05-31 Skapad: 2021-05-31 Senast uppdaterad: 2024-02-13Bibliografiskt granskad

Open Access i DiVA

fulltext(979 kB)304 nedladdningar
Filinformation
Filnamn FULLTEXT01.pdfFilstorlek 979 kBChecksumma SHA-512
6420987bb2f27625f4c5ede43d4999aa5cb9c21e7f753985959666fa79d79d3115cb219141ad3dd339714e3bef1a266da2f621b4ac030b039bcd6fb1afeaa951
Typ fulltextMimetyp application/pdf

Övriga länkar

Förlagets fulltextScopus

Person

Capo, EricKoizumi, ShuntaroPuts, IsoldeOlajos, FredrikKarlsson, JanByström, Pär

Sök vidare i DiVA

Av författaren/redaktören
Capo, EricKoizumi, ShuntaroPuts, IsoldeOlajos, FredrikKarlsson, JanByström, Pär
Av organisationen
Institutionen för ekologi, miljö och geovetenskap
Evolutionsbiologi

Sök vidare utanför DiVA

GoogleGoogle Scholar
Totalt: 304 nedladdningar
Antalet nedladdningar är summan av nedladdningar för alla fulltexter. Det kan inkludera t.ex tidigare versioner som nu inte längre är tillgängliga.

doi
urn-nbn

Altmetricpoäng

doi
urn-nbn
Totalt: 409 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf