Umeå universitets logga

umu.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
The DrosDel collection: a set of P-element insertions for generating custom chromosomal aberrations in Drosophila melanogaster.
Visa övriga samt affilieringar
2004 (Engelska)Ingår i: Genetics, ISSN 0016-6731, Vol. 167, nr 2, s. 797-813Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

We describe a collection of P-element insertions that have considerable utility for generating custom chromosomal aberrations in Drosophila melanogaster. We have mobilized a pair of engineered P elements, p[RS3] and p[RS5], to collect 3243 lines unambiguously mapped to the Drosophila genome sequence. The collection contains, on average, an element every 35 kb. We demonstrate the utility of the collection for generating custom chromosomal deletions that have their end points mapped, with base-pair resolution, to the genome sequence. The collection was generated in an isogenic strain, thus affording a uniform background for screens where sensitivity to genetic background is high. The entire collection, along with a computational and genetic toolbox for designing and generating custom deletions, is publicly available. Using the collection it is theoretically possible to generate >12,000 deletions between 1 bp and 1 Mb in size by simple eye color selection. In addition, a further 37,000 deletions, selectable by molecular screening, may be generated. We are now using the collection to generate a second-generation deficiency kit that is precisely mapped to the genome sequence.

Ort, förlag, år, upplaga, sidor
2004. Vol. 167, nr 2, s. 797-813
Nyckelord [en]
Animals, Chromosome Aberrations, DNA Transposable Elements/*genetics, Drosophila melanogaster/*genetics, Genetic Techniques, Mutagenesis; Insertional/methods
Identifikatorer
URN: urn:nbn:se:umu:diva-16827PubMedID: 15238529Scopus ID: 2-s2.0-2942743598OAI: oai:DiVA.org:umu-16827DiVA, id: diva2:156500
Tillgänglig från: 2007-10-12 Skapad: 2007-10-12 Senast uppdaterad: 2023-03-24Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

PubMedScopushttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=15238529&dopt=Citation

Person

Rasmuson-Lestander, ÅsaEkström, Karin

Sök vidare i DiVA

Av författaren/redaktören
Rasmuson-Lestander, ÅsaEkström, Karin
Av organisationen
Molekylärbiologi (Teknisk-naturvetenskaplig fakultet)

Sök vidare utanför DiVA

GoogleGoogle Scholar

pubmed
urn-nbn

Altmetricpoäng

pubmed
urn-nbn
Totalt: 431 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf