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Induction of Epithelial-mesenchymal Transition in MDCK II Cells
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB).ORCID iD: 0000-0002-9103-3419
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB).
2021 (English)In: Bio-protocol, E-ISSN 2331-8325, Vol. 11, no 3, article id e3903Article in journal (Refereed) Published
Abstract [en]

Epithelial-mesenchymal transition (EMT) is a reversible process of epithelial cell transdifferentiation into a mesenchymal cell, that enables initiation of cell migration. EMT plays an important role in embryonic development, tissue repair and cancer metastasis. Better understanding of cellular and molecular events during EMT will not only provide novel insights on how mammalian organism develops and how epithelial tissues regenerate, but also can identify novel therapeutic targets for cancer therapy. Here we aim to provide a detailed protocol on how to induce EMT in Madin-Darby Canine Kidney (MDCK) II epithelial cell line and perform immunofluorescent staining on EMT-induced cells.

Place, publisher, year, edition, pages
Bio-protocol , 2021. Vol. 11, no 3, article id e3903
Keywords [en]
Epithelial-mesenchymal transition, MDCK cells, Hepatocyte growth factor, Epithelial plasticity, EMT, Mesenchymal cell differentiation from epithelial cell
National Category
Cancer and Oncology Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-187355DOI: 10.21769/BioProtoc.3903ISI: 000616641600003PubMedID: 33732790Scopus ID: 2-s2.0-85116018142OAI: oai:DiVA.org:umu-187355DiVA, id: diva2:1592475
Funder
Swedish Cancer Society, CAN2017/735Available from: 2021-09-09 Created: 2021-09-09 Last updated: 2023-03-24Bibliographically approved

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Pastula, AgnieszkaLundmark, Richard

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CiteExportLink to record
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