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An atlas of the Norway spruce needle seasonal transcriptome
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).ORCID iD: 0000-0003-3858-4606
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).ORCID iD: 0000-0001-7979-8876
Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC). Swedish University of Agricultural Sciences (SLU), Umeå, Sweden.ORCID iD: 0000-0002-3053-0796
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
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2021 (English)In: The Plant Journal, ISSN 0960-7412, E-ISSN 1365-313X, Vol. 108, no 6, p. 1815-1829Article in journal (Refereed) Published
Abstract [en]

Boreal conifers possess a tremendous ability to survive and remain evergreen during harsh winter conditions and resume growth during summer. This is enabled by coordinated regulation of major cellular functions at the level of gene expression, metabolism, and physiology. Here we present a comprehensive characterization of the annual changes in the global transcriptome of Norway spruce (Picea abies) needles as a resource to understand needle development and acclimation processes throughout the year. In young, growing needles (May 15 until June 30), cell walls, organelles, etc., were formed, and this developmental program heavily influenced the transcriptome, explained by over-represented Gene Ontology (GO) categories. Later changes in gene expression were smaller but four phases were recognized: summer (July–August), autumn (September–October), winter (November–February), and spring (March–April), where over-represented GO categories demonstrated how the needles acclimated to the various seasons. Changes in the seasonal global transcriptome profile were accompanied by differential expression of members of the major transcription factor families. We present a tentative model of how cellular activities are regulated over the year in needles of Norway spruce, which demonstrates the value of mining this dataset, accessible in ConGenIE together with advanced visualization tools.

Place, publisher, year, edition, pages
John Wiley & Sons, 2021. Vol. 108, no 6, p. 1815-1829
Keywords [en]
conifers, Norway spruce, resource, seasonal adaptation, transcriptomics
National Category
Botany
Identifiers
URN: urn:nbn:se:umu:diva-188962DOI: 10.1111/tpj.15530ISI: 000709512700001PubMedID: 34624161Scopus ID: 2-s2.0-85117463119OAI: oai:DiVA.org:umu-188962DiVA, id: diva2:1606843
Funder
EU, Horizon 2020, 675006VinnovaSwedish Research Council FormasKnut and Alice Wallenberg FoundationSwedish Research Council, 2018‐05973The Kempe FoundationsAvailable from: 2021-10-28 Created: 2021-10-28 Last updated: 2023-03-24Bibliographically approved
In thesis
1. How could Christmas trees remain evergreen?: photosynthetic acclimation of Scots pine and Norway spruce needles during winter
Open this publication in new window or tab >>How could Christmas trees remain evergreen?: photosynthetic acclimation of Scots pine and Norway spruce needles during winter
2022 (English)Doctoral thesis, comprehensive summary (Other academic)
Alternative title[sv]
Hur kan julgranen vara grön? : fotosyntesapparatens anpassning till vinterförhållanden hos tall och gran
Abstract [en]

Plants and other green organisms harvest sunlight by green chlorophyll pigments and covertit to chemical energy (sugars) and oxygen in a process called photosynthesis providing the foundation for life on Earth. Although it is unanimously believed that oceanic phytoplanktons are the main contributors to the global photosynthesis, the contribution of coniferous boreal forests distributed across vast regions of the northern hemisphere cannot be undermined. Hence boreal forests account signifificantly for social, economical and environmental sustainability. Not only do conifers thrive in the tundra regions with extreme climate, but they also maintain their needles green over the boreal winter. A question remains; what makes them so resilient? In this respect, we aimed to understand the remarkable winter adaptation strategies in two dominant boreal coniferous species,i.e., Pinus sylvestris and Picea abies. First, we mapped the transcriptional landscape in Norway spruce (Picea abies) needles over the annual cycle. Transcriptional changes in the nascent needles reflflected a sequence of developmental processes and active vegetative growth during early summer and summer. Later after maturation, transcriptome reflflected activated defense against biotic factors and acclimationin response to abiotic environmental cues such as freezing temperatures during winter. Secondly, by monitoring the photosynthetic performance of Scot pine needles, we found that the trees face extreme stress during the early spring (Feb-Mar) when sub-zero temperatures are accompanied by high solar radiation. At this time, drastic changes occur in the thylakoid membranes of the chloroplast that allows the mixing of photosystem I and photosystem II that typically remain laterally segregated. This triggers direct energy transfer from PSII to PSI and thus protects PSII from damage. Furthermore, we found that this loss of lateral segregation may be a consequence of triple phosphorylationof Lhcb1 (Light harvesting complex1 of photosystem II). The structural changes in thylakoid membranes also lead to changes inthe thylakoid macro domain organisationand pigment protein composition. Furthermore, we discovered that while PSII is protected by direct energy transfer, the protection of PSI is provided through photoreduction of oxygen by flavodiiron proteins, which in turn allows P700 to stay in an oxidised state necessary for direct energy transfer. These coordinated cascades of changes concomitantly protect both PSI and PSII to maintain the needles green over the winter.

Place, publisher, year, edition, pages
Umeå: Umeå University, 2022. p. 65
Keywords
Scots Pine, Norway spruce, Photosynthesis, Winter adaptation
National Category
Biochemistry and Molecular Biology Bioinformatics and Systems Biology Botany Biophysics Evolutionary Biology
Research subject
biological chemistry; biology; Molecular Biology; Physiological Botany
Identifiers
urn:nbn:se:umu:diva-194032 (URN)978-91-7855-788-2 (ISBN)978-91-7855-787-5 (ISBN)
Public defence
2022-05-20, Lilla hörsalen, KBC huset, Umeå, 09:30 (English)
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Supervisors
Note

On page 12, List of papers, manuscripts (in preparation): Athor names hidden due to copyright - see printed version. 

Available from: 2022-04-29 Created: 2022-04-22 Last updated: 2022-05-06Bibliographically approved

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Bag, PushanLihavainen, JennaDelhomme, NicolasRiquelme, ThomasRobinson, Kathryn M.Jansson, Stefan

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Bag, PushanLihavainen, JennaDelhomme, NicolasRiquelme, ThomasRobinson, Kathryn M.Jansson, Stefan
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