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Insulin-Binding Peptide Probes Provide a Novel Strategy for Pancreatic β-Cell Imaging
Umeå University, Faculty of Medicine, Umeå Centre for Molecular Medicine (UCMM).
St Vincent's Institute of Medical Research, 9 Princes Street, Melbourne, Australia.
Charles Perkins Centre, Discipline of Physiology, School of Medical Sciences, University of Sydney, Johns Hopkins Dr, Sydney, Australia.
St Vincent's Institute of Medical Research, 9 Princes Street, Melbourne, Australia; Department of Medicine, The University of Melbourne, Parkville, Melbourne, Australia.
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2021 (English)In: Molecular Pharmaceutics, ISSN 1543-8384, E-ISSN 1543-8392, Vol. 18, no 12, p. 4428-4436Article in journal (Refereed) Published
Abstract [en]

Type 1 diabetes develops in childhood and adolescence, with peak incidence in the early teenage years. There is an urgent need for an accurate method to detect insulin-producing β-cells in patients that is not affected by alterations in β-cell function. As part of our research program to design specific probes to measure β-cell mass, we recently developed a novel insulin-binding peptide probe (IBPP) for the detection of β-cells in vivo. Here, we applied our innovative method to show specific labeling of this IBPP to human and mouse fixed β-cells in pancreatic islets. Importantly, we showed staining of human and mouse islets in culture without any negative functional or cell viability impact. Moreover, the IBPP-stained mouse islets after tail vein injection in vivo, albeit with batch differences in staining efficiency. In conclusion, we provide evidence showing that the IBPP can be used for future accurate detection of β-cell mass in a variety of preclinical models of diabetes.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2021. Vol. 18, no 12, p. 4428-4436
Keywords [en]
glucose-stimulated insulin secretion, insulin-binding peptide, pancreatic islets, type 1 diabetes, β-cell imaging
National Category
Endocrinology and Diabetes Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-189219DOI: 10.1021/acs.molpharmaceut.1c00616ISI: 000755047200018Scopus ID: 2-s2.0-85118183928OAI: oai:DiVA.org:umu-189219DiVA, id: diva2:1610274
Funder
EU, FP7, Seventh Framework Programme, 289932Swedish Research CouncilNovo NordiskAvailable from: 2021-11-10 Created: 2021-11-10 Last updated: 2023-09-05Bibliographically approved

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Eriksson, MariaAhlgren, Ulf

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