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DamID transcriptional profiling identifies the Snail/Scratch transcription factor Kahuli as an Alk target in the Drosophila visceral mesoderm
Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, University of Gothenburg, SE-405 30 Gothenburg, Sweden.ORCID-id: 0000-0002-6084-7962
Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, University of Gothenburg, SE-405 30 Gothenburg, Sweden.ORCID-id: 0000-0001-6107-2487
Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, University of Gothenburg, SE-405 30 Gothenburg, Sweden.ORCID-id: 0000-0001-9543-3113
Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, University of Gothenburg, SE-405 30 Gothenburg, Sweden.
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2021 (Engelska)Ingår i: Development, ISSN 0950-1991, E-ISSN 1477-9129, Vol. 148, nr 23, artikel-id dev199465Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Development of the Drosophila visceral muscle depends on Anaplastic Lymphoma Kinase (Alk) receptor tyrosine kinase (RTK) signaling, which specifies founder cells (FCs) in the circular visceral mesoderm (VM). Although Alk activation by its ligand Jelly Belly (Jeb) is well characterized, few target molecules have been identified. Here, we used targeted DamID (TaDa) to identify Alk targets in embryos overexpressing Jeb versus embryos with abrogated Alk activity, revealing differentially expressed genes, including the Snail/Scratch family transcription factor Kahuli (Kah). We confirmed Kah mRNA and protein expression in the VM, and identified midgut constriction defects in Kah mutants similar to those of pointed (pnt). ChIP and RNA-Seq data analysis defined a Kah target-binding site similar to that of Snail, and identified a set of common target genes putatively regulated by Kah and Pnt during midgut constriction. Taken together, we report a rich dataset of Alk-responsive loci in the embryonic VM and functionally characterize the role of Kah in the regulation of embryonic midgut morphogenesis.

Ort, förlag, år, upplaga, sidor
The Company of Biologists , 2021. Vol. 148, nr 23, artikel-id dev199465
Nyckelord [en]
Single cell, ChIP, Jelly belly, Signaling, TaDa, Midgut constriction, ETS, Pointed
Nationell ämneskategori
Genetik Biokemi och molekylärbiologi Cell- och molekylärbiologi
Identifikatorer
URN: urn:nbn:se:umu:diva-190470DOI: 10.1242/dev.199465ISI: 000731753800002PubMedID: 34905617Scopus ID: 2-s2.0-85122474160OAI: oai:DiVA.org:umu-190470DiVA, id: diva2:1620664
Forskningsfinansiär
Cancerfonden, RHP CAN18/0729Cancerfonden, BA 200270PCancerfonden, JLA CAN17/342Cancerfonden, EL CAN15/541Barncancerfonden, RHP 2019-0078Vetenskapsrådet, RHP 2019-03914Vetenskapsrådet, MB 2019-01708Vetenskapsrådet, JLA 2016-03306Vetenskapsrådet, EL 14-3596Knut och Alice Wallenbergs Stiftelse, KAW 2015.0144Tillgänglig från: 2021-12-16 Skapad: 2021-12-16 Senast uppdaterad: 2023-03-24Bibliografiskt granskad

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Lindehell, HenrikLarsson, Jan

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Mendoza-Garcia, PatriciaBasu, SwarajSukumar, Sanjay KumarLindehell, HenrikLarsson, JanBemark, MatsPalmer, Ruth H.
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Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet)
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GenetikBiokemi och molekylärbiologiCell- och molekylärbiologi

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