Umeå University's logo

umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Microstructured collagen films for 3D corneal stroma modelling
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB). (Chondrogenic and osteogenic differentiation group)ORCID iD: 0000-0003-2911-2981
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB).ORCID iD: 0000-0002-1617-334X
Umeå University, Faculty of Medicine, Department of Clinical Microbiology. Umeå University, Faculty of Medicine, Wallenberg Centre for Molecular Medicine at Umeå University (WCMM).ORCID iD: 0000-0003-0634-7091
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB). Umeå University, Faculty of Medicine, Department of Community Medicine and Rehabilitation.ORCID iD: 0000-0002-6091-3982
Show others and affiliations
2022 (English)In: Connective Tissue Research, ISSN 0300-8207, E-ISSN 1607-8438, Vol. 63, no 5, p. 443-452Article in journal (Refereed) Published
Abstract [en]

Purpose/aim: Corneal injury is a major cause of impaired vision around the globe. The fine structure of the corneal stroma plays a pivotal role in the phenotype and behavior of the embedded cells during homeostasis and healing after trauma or infection. In order to study healing processes in the cornea, it is important to create culture systems that functionally mimic the natural environment.

Materials and methods: Collagen solution was vitrified on top of a grated film to achieve thin collagen films with parallel microgrooves. Keratocytes (corneal stromal cells) were cultured on the films either as a single layer or as stacked layers of films and cells. SEM and F-actin staining were used to analyze the pattern transference onto the collagen and the cell orientation on the films. Cell viability was analyzed with MTS and live/dead staining. Keratocytes, fibroblasts, and myofibroblasts were cultured to study the pattern’s effect on phenotype.

Results: A microstructured collagen film-based culture system that guides keratocytes (stromal cells) to their native, layerwise perpendicular orientation in 3D and that can support fibroblasts and myofibroblasts was created. The films are thin and transparent enough to observe cells at least three layers deep. The cells maintain viability in 2D and 3D cultures and the films can support fibroblast and myofibroblast phenotypes.

Conclusions: The films provide an easily reproducible stroma model that maintains high cell viability and improves the preservation of the keratocyte phenotype in keratocytes that are differentiated to fibroblasts.

Place, publisher, year, edition, pages
Taylor & Francis Group, 2022. Vol. 63, no 5, p. 443-452
Keywords [en]
collagen, cornea, keratocyte, stroma, Vitrigel
National Category
Ophthalmology
Identifiers
URN: urn:nbn:se:umu:diva-190877DOI: 10.1080/03008207.2021.2007901ISI: 000729669400001PubMedID: 34894951Scopus ID: 2-s2.0-85121425675OAI: oai:DiVA.org:umu-190877DiVA, id: diva2:1623499
Funder
Stiftelsen Kronprinsessan Margaretas arbetsnämnd för synskadade, 2013/10Swedish Society of Medicine, 504541Swedish Research Council, 2017-01138Region Västerbotten, 549761Available from: 2021-12-29 Created: 2021-12-29 Last updated: 2024-07-02Bibliographically approved

Open Access in DiVA

fulltext(5124 kB)147 downloads
File information
File name FULLTEXT02.pdfFile size 5124 kBChecksum SHA-512
b90eacf01e29a43ba90bff71b84f45de8dcae7fca2cdf593d42c3cd96dee8782db0d34699b34e1575a85fac5343bb4cf5a84ec02101f5b22a93edc1026851cf8
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMedScopus

Authority records

Prittinen, JuhaZhou, XinBano, FouziaBackman, Ludvig J.Danielson, Patrik

Search in DiVA

By author/editor
Prittinen, JuhaZhou, XinBano, FouziaBackman, Ludvig J.Danielson, Patrik
By organisation
Department of Integrative Medical Biology (IMB)Department of Clinical MicrobiologyWallenberg Centre for Molecular Medicine at Umeå University (WCMM)Department of Community Medicine and RehabilitationOphthalmology
In the same journal
Connective Tissue Research
Ophthalmology

Search outside of DiVA

GoogleGoogle Scholar
Total: 188 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 354 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf