Umeå University's logo

umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Cryo-XPS spectra from bacterial reference strain Pseudomonas fluorescens DSM50090
Umeå University, Faculty of Science and Technology, Department of Chemistry.ORCID iD: 0000-0003-2646-8501
Umeå University, Faculty of Science and Technology, Department of Chemistry.ORCID iD: 0000-0002-4766-2672
2022 (English)In: Surface Science Spectra, ISSN 1055-5269, E-ISSN 1520-8575, Vol. 29, no 1, article id 014010Article in journal (Refereed) Published
Abstract [en]

Cryogenic x-ray photoelectron spectroscopy was used to analyze the cell envelope of intact and hydrated Gram-negative bacteria of the species Pseudomonas fluorescens. We used a reference strain, DSM50090, from the German microbial culture collection, which we previously have suggested would function well as a reference strain for future XPS analyses of Gram-negative bacteria. Bacteria were grown on nutrient agar plates at room temperature, collected with a cultivation loop, and washed using phosphate buffered saline. An aliquot of the cell pellet was fast-frozen on the sample holder in the sample introduction chamber to a temperature of 103 K and kept frozen throughout the measurement. Survey spectra and high-resolution spectra of Na 1s, O 1s, N 1s, C 1s, Cl 2p, S 2p, and P 2p are reported. The spectra obtained from the analyzed cells represent a combined signal from O, N, C, and S atoms in proteins, lipids, and polysaccharides at the cell surface. Furthermore, signal from P, Na, K, and Cl atoms was present both originating from processes in the cell envelope and remnants from the wash buffer.

Place, publisher, year, edition, pages
AVS Science and Technology Society , 2022. Vol. 29, no 1, article id 014010
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:umu:diva-193343DOI: 10.1116/6.0001575ISI: 000780202400001Scopus ID: 2-s2.0-85127307826OAI: oai:DiVA.org:umu-193343DiVA, id: diva2:1647534
Available from: 2022-03-28 Created: 2022-03-28 Last updated: 2023-03-24Bibliographically approved

Open Access in DiVA

fulltext(2329 kB)89 downloads
File information
File name FULLTEXT02.pdfFile size 2329 kBChecksum SHA-512
81216a8e6c43655e60617ab54296bdf840d7bc4f416fd13d1d2847523121bf8f55d6690ec47c4d6814be53d2a53ca6a3be03d051ea01662ee1f4ab849d332324
Type fulltextMimetype application/pdf

Other links

Publisher's full textScopus

Authority records

Ramstedt, MadeleineShchukarev, Andrey

Search in DiVA

By author/editor
Ramstedt, MadeleineShchukarev, Andrey
By organisation
Department of Chemistry
In the same journal
Surface Science Spectra
Analytical Chemistry

Search outside of DiVA

GoogleGoogle Scholar
Total: 238 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 340 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf