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Cpx-signalling facilitates Hms-dependent biofilm formation by Yersinia pseudotuberculosis
Umeå University, Faculty of Science and Technology, Department of Molecular Biology (Faculty of Science and Technology). Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR). (Matthew S. Francis)ORCID iD: 0000-0001-6963-0009
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR). Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS). (Sun Nyunt Wai)ORCID iD: 0000-0003-4793-4671
Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, USA. (David Erickson)ORCID iD: 0000-0001-6149-273X
Umeå University, Faculty of Science and Technology, Department of Molecular Biology (Faculty of Science and Technology). Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR). (Matthew Francis)ORCID iD: 0000-0001-6817-9535
2022 (English)In: npj Biofilms and Microbiomes, E-ISSN 2055-5008, Vol. 8, no 1, article id 13Article in journal (Refereed) Published
Abstract [en]

Bacteria often reside in sessile communities called biofilms, where they adhere to a variety of surfaces and exist as aggregates in aviscous polymeric matrix. Biofilms are resistant to antimicrobial treatments, and are a major contributor to the persistence and chronicity of many bacterial infections. Herein, we determined that the CpxA-CpxR two-component system influenced the ability of enteropathogenic Yersinia pseudotuberculosis to develop biofilms. Mutant bacteria that accumulated the active CpxR~P isoform failed to form biofilms on plastic or on the surface of the Caenorhabditis elegans nematode. A failure to form biofilms on the worm surface prompted their survival when grown on the lawns of Y. pseudotuberculosis. Exopolysaccharide production by the hms loci is the major driver of biofilms formed by Yersinia. We used a number of molecular genetic approaches to demonstrate that active CpxR~P binds directly to the promoter regulatory elements of the hms loci to activate the repressors of hms expression and to repress the activators of hms expression. Consequently, active Cpx-signalling culminated in a loss of exopolysaccharide production. Hence, the development of Y. pseudotuberculosis biofilms on multiple surfaces is controlled by the Cpx-signalling, and at least inpart this occurs through repressive effects on the Hms-dependent exopolysaccharide production.

Place, publisher, year, edition, pages
London: Nature Publishing Group, 2022. Vol. 8, no 1, article id 13
Keywords [en]
Biofilms, Microbial genetics, Two-component system, CpxAR, Yersinia pseudotuberculosis, Caenorhabditis elegans
National Category
Microbiology Microbiology in the medical area
Research subject
Microbiology; molecular medicine (genetics and pathology); Biochemistry; Genetics; Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-193376DOI: 10.1038/s41522-022-00281-4ISI: 000774860800001PubMedID: 35351893Scopus ID: 2-s2.0-85127231602OAI: oai:DiVA.org:umu-193376DiVA, id: diva2:1648118
Funder
Swedish Research Council, 2014-06652Available from: 2022-03-29 Created: 2022-03-29 Last updated: 2023-09-05Bibliographically approved

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Kumar Gahlot, DharmenderWai, Sun NyuntFrancis, Matthew S.

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Kumar Gahlot, DharmenderWai, Sun NyuntErickson, David L.Francis, Matthew S.
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Department of Molecular Biology (Faculty of Science and Technology)Umeå Centre for Microbial Research (UCMR)Department of Molecular Biology (Faculty of Medicine)Molecular Infection Medicine Sweden (MIMS)
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MicrobiologyMicrobiology in the medical area

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