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Fluorescent Molecularly Imprinted Polymer Layers against Sialic Acid on Silica-Coated Polystyrene Cores — Assessment of the Binding Behavior to Cancer Cells
Department of Biomedical Sciences, Faculty of Health and Society, Malmö University, Malmö, Sweden.
Chemical and Optical Sensing Division, Bundesanstalt für Materialforschung und-prüfung (BAM), Richard-Willstätter Straße 11, Berlin, Germany.
Department of Biomedical Sciences, Faculty of Health and Society, Malmö University, Malmö, Sweden; Biofilms-Research Center for Biointerfaces, Malmö University, Malmö, Sweden.
Institute of Biomedicine, University of Turku, Turku, Finland; FICAN West Cancer Centre, Turku University Hospital, Turku, Finland.
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2022 (English)In: Cancers, ISSN 2072-6694, Vol. 14, no 8, article id 1875Article in journal (Refereed) Published
Abstract [en]

Sialic acid (SA) is a monosaccharide usually linked to the terminus of glycan chains on the cell surface. It plays a crucial role in many biological processes, and hypersialylation is a common feature in cancer. Lectins are widely used to analyze the cell surface expression of SA. However, these protein molecules are usually expensive and easily denatured, which calls for the development of alternative glycan-specific receptors and cell imaging technologies. In this study, SA-imprinted fluorescent core-shell molecularly imprinted polymer particles (SA-MIPs) were employed to recognize SA on the cell surface of cancer cell lines. The SA-MIPs improved suspensibility and scattering properties compared with previously used core-shell SA-MIPs. Although SA-imprinting was performed using SA without preference for the α2,3-and α2,6-SA forms, we screened the cancer cell lines analyzed using the lectins Maackia Amurensis Lectin I (MAL I, α2,3-SA) and Sambucus Nigra Lectin (SNA, α2,6-SA). Our results show that the selected cancer cell lines in this study presented a varied binding behavior with the SA-MIPs. The binding pattern of the lectins was also demonstrated. Moreover, two different pentavalent SA conjugates were used to inhibit the binding of the SA-MIPs to breast, skin, and lung cancer cell lines, demonstrating the specificity of the SA-MIPs in both flow cytometry and confocal fluorescence microscopy. We concluded that the synthesized SA-MIPs might be a powerful future tool in the diagnostic analysis of various cancer cells.

Place, publisher, year, edition, pages
MDPI, 2022. Vol. 14, no 8, article id 1875
Keywords [en]
cancer, imprinting, molecularly imprinted polymers, SA conjugates, sialic acid
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-194273DOI: 10.3390/cancers14081875ISI: 000786858400001Scopus ID: 2-s2.0-85127781436OAI: oai:DiVA.org:umu-194273DiVA, id: diva2:1654958
Available from: 2022-04-29 Created: 2022-04-29 Last updated: 2023-09-05Bibliographically approved

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Wang, TianyanPersson, Jenny L.Johansson, EmilCaraballo, RemiElofsson, Mikael

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