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Combined Transcriptomic and Protein Array Cytokine Profiling of Human Stem Cells from Dental Apical Papilla Modulated by Oral Bacteria
Umeå University, Faculty of Medicine, Department of Odontology. Department of Microbiology, Virology and Biotechnology, Mechnikov National University, Odesa, Ukraine.
Department of Microbiology, Virology and Biotechnology, Mechnikov National University, Odesa, Ukraine.
Umeå University, Faculty of Medicine, Department of Odontology.ORCID iD: 0000-0002-3536-4467
Umeå University, Faculty of Medicine, Department of Medical Biosciences, Pathology.
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2022 (English)In: International Journal of Molecular Sciences, ISSN 1661-6596, E-ISSN 1422-0067, Vol. 23, no 9, article id 5098Article in journal (Refereed) Published
Abstract [en]

Stem cells from the apical papilla (SCAP) are a promising resource for use in regenerative endodontic treatment (RET) that may be adversely affected by oral bacteria, which in turn can exert an effect on the success of RET. Our work aims to study the cytokine profile of SCAP upon exposure to oral bacteria and their supernatants—Fusobacterium nucleatum and Enterococcus faecalis—as well as to establish their effect on the osteogenic and immunogenic potentials of SCAP. Further, we target the presence of key proteins of the Wnt/β-Catenin, TGF-β, and NF-κB signaling pathways, which play a crucial role in adult osteogenic differentiation of mesenchymal stem cells, using the Western blot (WB) technique. The membrane-based sandwich immunoassay and transcriptomic analysis showed that, under the influence of F. nucleatum (both bacteria and supernatant), the production of pro-inflammatory cytokines IL-6, IL-8, and MCP-1 occurred, which was also confirmed at the mRNA level. Conversely, E. faecalis reduced the secretion of the aforementioned cytokines at both mRNA and protein levels. WB analysis showed that SCAP co-cultivation with E. faecalis led to a decrease in the level of the key proteins of the Wnt/β-Catenin and NF-κB signaling pathways: β-Catenin (p = 0.0068 *), LRP-5 (p = 0.0059 **), and LRP-6 (p = 0.0329 *), as well as NF-kB (p = 0.0034 **) and TRAF6 (p = 0.0285 *). These results suggest that oral bacteria can up-and downregulate the immune and inflammatory responses of SCAP, as well as influence the osteogenic potential of SCAP, which may negatively regulate the success of RET.

Place, publisher, year, edition, pages
MDPI, 2022. Vol. 23, no 9, article id 5098
Keywords [en]
cytokine secretion, endodontics, Fusobacterium nucleatum, IL-6, IL-8, immune response, osteogenic potential, regenerative endodontic treatment (RET), stem cells from the apical papilla (SCAP), transcriptome analysis
National Category
Dentistry Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-194879DOI: 10.3390/ijms23095098ISI: 000799319900001PubMedID: 35563488Scopus ID: 2-s2.0-85129389469OAI: oai:DiVA.org:umu-194879DiVA, id: diva2:1666791
Funder
Swedish Research Council, 2018-05973Knut and Alice Wallenberg Foundation, 7003503Region Västerbotten, 7004361The Kempe Foundations, SMK-1966Region Västerbotten, 7003459Region Västerbotten, 7003589Available from: 2022-06-09 Created: 2022-06-09 Last updated: 2023-05-23Bibliographically approved

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Zymovets, ValeriiaRakhimova, OlenaAripaka, KarthikKelk, PeymanLandström, MaréneRomani Vestman, Nelly

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Zymovets, ValeriiaRakhimova, OlenaAripaka, KarthikKelk, PeymanLandström, MaréneRomani Vestman, Nelly
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Department of OdontologyPathologyAnatomyWallenberg Centre for Molecular Medicine at Umeå University (WCMM)
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