Umeå University's logo

umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
The RPN12a proteasome subunit is essential for the multiple hormonal homeostasis controlling the progression of leaf senescence
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).ORCID iD: 0000-0001-8313-3535
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).ORCID iD: 0000-0003-3858-4606
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).ORCID iD: 0000-0003-1093-3317
Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, Umeå, Sweden.
Show others and affiliations
2022 (English)In: Communications Biology, E-ISSN 2399-3642, Vol. 5, no 1, article id 1043Article in journal (Refereed) Published
Abstract [en]

The 26S proteasome is a conserved multi-subunit machinery in eukaryotes. It selectively degrades ubiquitinated proteins, which in turn provides an efficient molecular mechanism to regulate numerous cellular functions and developmental processes. Here, we studied a new loss-of-function allele of RPN12a, a plant ortholog of the yeast and human structural component of the 19S proteasome RPN12. Combining a set of biochemical and molecular approaches, we confirmed that a rpn12a knock-out had exacerbated 20S and impaired 26S activities. The altered proteasomal activity led to a pleiotropic phenotype affecting both the vegetative growth and reproductive phase of the plant, including a striking repression of leaf senescence associate cell-death. Further investigation demonstrated that RPN12a is involved in the regulation of several conjugates associated with the auxin, cytokinin, ethylene and jasmonic acid homeostasis. Such enhanced aptitude of plant cells for survival in rpn12a contrasts with reports on animals, where 26S proteasome mutants generally show an accelerated cell death phenotype.

Place, publisher, year, edition, pages
Nature Publishing Group, 2022. Vol. 5, no 1, article id 1043
National Category
Botany Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-200406DOI: 10.1038/s42003-022-03998-2ISI: 000862402500001PubMedID: 36180574Scopus ID: 2-s2.0-85139221413OAI: oai:DiVA.org:umu-200406DiVA, id: diva2:1705227
Funder
The Kempe FoundationsCarl Tryggers foundation , CTS2018-193Swedish Foundation for Strategic Research, FFF20-0008Knut and Alice Wallenberg FoundationVinnovaAvailable from: 2022-10-21 Created: 2022-10-21 Last updated: 2024-04-09Bibliographically approved

Open Access in DiVA

fulltext(4045 kB)80 downloads
File information
File name FULLTEXT01.pdfFile size 4045 kBChecksum SHA-512
b24cb21020944406949344b14ee494b849e964bf39733fec08fc9991d8ebd41753b058b2cd25912841330bdfa65cbd958d77718ce5f2cd14b5cf2a2f97c439f8
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMedScopus

Authority records

Boussardon, ClémentBag, PushanJuvany, MartaJansson, StefanKeech, Olivier

Search in DiVA

By author/editor
Boussardon, ClémentBag, PushanJuvany, MartaJansson, StefanKeech, Olivier
By organisation
Department of Plant PhysiologyUmeå Plant Science Centre (UPSC)
In the same journal
Communications Biology
BotanyBiochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 80 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 250 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf