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Decreased levels of T follicular helper (CD4+CXCR5+) cells and CD27+CD38+ and CD27+CD38− B cells in ankylosing spondylitis patients correlate with markers of inflammation
Umeå University, Faculty of Medicine, Department of Clinical Microbiology.ORCID iD: 0000-0001-5025-6539
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Section of Medicine. Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Rheumatology.ORCID iD: 0000-0002-3822-0725
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Rheumatology. Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Section of Medicine.
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Rheumatology. Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Section of Medicine. Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.ORCID iD: 0000-0001-7871-5303
2023 (English)In: Scandinavian Journal of Immunology, ISSN 0300-9475, E-ISSN 1365-3083, Vol. 97, no 1, article id e13235Article in journal (Refereed) Published
Abstract [en]

The purpose of this study was to study CD4+CXCR5+ T follicular helper (TFH) cells, CD27+CD38+ plasmablasts and CD27+CD38− memory B cells, as well as disease-related factors in patients with ankylosing spondylitis (AS) from northern Sweden. Peripheral blood mononuclear cells (PBMC) from 50 patients with AS (mean age 52 ± 9 years, 66% men, 100% HLA-B27 positive) and 50 pairwise matched blood donor controls (mean age 54 ± 9 years, 66% men) were stained with antibodies for CD27, CD38, CD19, CD3, CD4 and CXCR5 markers and analysed by flow cytometry. Patients with AS were examined with spinal x-ray for radiographic alterations (mSASSS), and plasma levels of C-reactive protein, erythrocyte sedimentation rate, as well as selected proinflammatory and regulatory cytokines were determined. Physical mobility, function and disease activity were registered by BASMI, BASFI and ASDAS-CRP, BASDAI, respectively. Comparing AS patients and controls pairwise, we observed a 56% reduction of TFH cells in PBMCs from AS patients (P =.000008). Furthermore, a 20%-30% reduction in plasmablasts and B memory cells (P ≤.002 and P ≤.007, respectively) was observed. In female patients, negative correlations between ESR and TFH, plasmablasts and B memory cells were observed; Rs = −0.551, P ≤.02; Rs = −0.476, P ≤.05 and Rs = −0.522, P ≤.03, respectively. In addition, positive correlations between the regulatory cytokine IL-10 and the proportion of B cells, IL-22, and the proportion of plasmablasts as well as a negative correlation between levels of the proinflammatory cytokine IL-6 and TFH were detected. Our observations indicate a role of an aberrant humoral immune response related to inflammation in AS.

Place, publisher, year, edition, pages
John Wiley & Sons, 2023. Vol. 97, no 1, article id e13235
Keywords [en]
ankylosing spondylitis, memory B cells, plasmablasts, radiographic axial spondyloarthritis, TFH
National Category
Immunology in the medical area Rheumatology and Autoimmunity
Identifiers
URN: urn:nbn:se:umu:diva-201478DOI: 10.1111/sji.13235ISI: 000889655600001Scopus ID: 2-s2.0-85142643347OAI: oai:DiVA.org:umu-201478DiVA, id: diva2:1716441
Funder
Stiftelsen Konung Gustaf V:s 80-årsfond, FAI2017-0454Region Västerbotten, ALFVLL-640251Swedish Research Council, 2016-02035Available from: 2022-12-06 Created: 2022-12-06 Last updated: 2023-03-24Bibliographically approved

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Lejon, KristinaHellman, UrbanKumar, AnjaniForsblad-d'Elia, Helena

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Immunology in the medical areaRheumatology and Autoimmunity

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