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Immune-activated B cells are dominant in prostate cancer
Umeå University, Faculty of Medicine, Department of Surgical and Perioperative Sciences, Urology and Andrology. Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Immunology/Immunchemistry.
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2023 (English)In: Cancers, ISSN 2072-6694, Vol. 15, no 3, article id 920Article in journal (Refereed) Published
Abstract [en]

B cells are multifaceted immune cells responding robustly during immune surveillance against tumor antigens by presentation to T cells and switched immunoglobulin production. However, B cells are unstudied in prostate cancer (PCa). We used flow cytometry to analyze B-cell subpopulations in peripheral blood and lymph nodes from intermediate-high risk PCa patients. B-cell subpopulations were related to clinicopathological factors. B-cell-receptor single-cell sequencing and VDJ analysis identified clonal B-cell expansion in blood and lymph nodes. Pathological staging was pT2 in 16%, pT3a in 48%, and pT3b in 36%. Lymph node metastases occurred in 5/25 patients (20%). Compared to healthy donors, the peripheral blood CD19+ B-cell compartment was significantly decreased in PCa patients and dominated by naïve B cells. The nodal B-cell compartment had significantly increased fractions of CD19+ B cells and switched memory B cells. Plasmablasts were observed in tumor-draining sentinel lymph nodes (SNs). VDJ analysis revealed clonal expansion in lymph nodes. Thus, activated B cells are increased in SNs from PCa patients. The increased fraction of switched memory cells and plasmablasts together with the presence of clonally expanded B cells indicate tumor-specific T-cell-dependent responses from B cells, supporting an important role for B cells in the protection against tumors.

Place, publisher, year, edition, pages
MDPI, 2023. Vol. 15, no 3, article id 920
Keywords [en]
B cells, T cells, prostatic neoplasms, sentinel lymph node biopsy
National Category
Urology and Nephrology
Identifiers
URN: urn:nbn:se:umu:diva-204791DOI: 10.3390/cancers15030920ISI: 000933775500001PubMedID: 36765877Scopus ID: 2-s2.0-85147818726OAI: oai:DiVA.org:umu-204791DiVA, id: diva2:1736359
Funder
Swedish Research Council, 2022-5-18The Kempe FoundationsAvailable from: 2023-02-13 Created: 2023-02-13 Last updated: 2024-02-01Bibliographically approved

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Banday, Viqar ShowkatSherif, Amir

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