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Widefield mid-infrared photothermal heterodyne imaging in the cell-silent window
Umeå University, Faculty of Science and Technology, Department of Applied Physics and Electronics.
Umeå University, Faculty of Science and Technology, Department of Applied Physics and Electronics.ORCID iD: 0000-0002-5065-7786
2023 (English)In: Advanced chemical microscopy for life science and translational medicine 2023 / [ed] Ji-Xin Cheng; Wei Min; Garth J. Simpson, SPIE Digital Library , 2023, article id 1239207Conference paper, Published paper (Refereed)
Abstract [en]

Widefield mid-infrared photothermal heterodyne (WIPH) microscopy enables sensitive and fast chemical imaging with high spatial resolution. The technique is realized using an external-cavity quantum cascade laser emitting and a digital frequency-domain lock-in filter for simultaneous multi-harmonic demodulation of WIPH signals recorded by individual camera pixels at a frame rate of 20 kHz. The filter allows the use of continuous-wave probe light and the time-resolved detection of photothermal decay curves. The microscope provides <1 µm spatial resolution in a 64x64 µm field of view. Here, we present preliminary results from hyperspectral WIPH imaging of alkyne-tagged palmitic acid (PA), azidetagged PA and perdeuterated PA via their absorption features in the cell-silent spectral region around 2100 cm-1. The alkyne and azide functional groups and deuterium are promising vibrational probes for selective imaging of biomolecules, such as lipids and proteins, in cells.

Place, publisher, year, edition, pages
SPIE Digital Library , 2023. article id 1239207
National Category
Atom and Molecular Physics and Optics Analytical Chemistry Medical Engineering
Identifiers
URN: urn:nbn:se:umu:diva-205830DOI: 10.1117/12.2654181Scopus ID: 2-s2.0-85159790180OAI: oai:DiVA.org:umu-205830DiVA, id: diva2:1744856
Conference
SPIE BIOS, San Francisco, Californa, USA, January 28 - February 3, 2023
Funder
The Kempe FoundationsSwedish Research CouncilUmeå UniversityAvailable from: 2023-03-21 Created: 2023-03-21 Last updated: 2023-06-21Bibliographically approved

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Maia Paiva, EduardoSchmidt, Florian

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