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Dynamin2 functions as an accessory protein to reduce the rate of caveola internalization
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB). Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR).
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB). Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR).
Institute for Molecular Bioscience, The University of Queensland, QLD, Brisbane, Australia.
Institute for Molecular Bioscience, The University of Queensland, QLD, Brisbane, Australia; Centre for Microscopy and Microanalysis, The University of Queensland, QLD, Brisbane, Australia.
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2023 (English)In: Journal of Cell Biology, ISSN 0021-9525, E-ISSN 1540-8140, Vol. 222, no 4, article id e202205122Article in journal (Refereed) Published
Abstract [en]

Caveolae are small membrane invaginations that generally are stably attached to the plasma membrane. Their release is believed to depend on the GTPase dynamin 2 (Dyn2), in analogy with its role in fission of clathrin-coated vesicles. The mechanistic understanding of caveola fission is, however, sparse. Here, we used microscopy-based tracking of individual caveolae in living cells to determine the role of Dyn2 in caveola dynamics. We report that Dyn2 stably associated with the bulb of a subset of caveolae, but was not required for formation or fission of caveolae. Dyn2-positive caveolae displayed longer plasma membrane duration times, whereas depletion of Dyn2 resulted in shorter duration times and increased caveola fission. The stabilizing role of Dyn2 was independent of its GTPase activity and the caveola stabilizing protein EHD2. Thus, we propose that, in contrast to the current view, Dyn2 is not a core component of the caveolae machinery, but rather functions as an accessory protein that restrains caveola internalization.

Place, publisher, year, edition, pages
Rockefeller University Press, 2023. Vol. 222, no 4, article id e202205122
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Biochemistry and Molecular Biology
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URN: urn:nbn:se:umu:diva-208218DOI: 10.1083/jcb.202205122ISI: 000978090900001PubMedID: 36729022Scopus ID: 2-s2.0-85153874757OAI: oai:DiVA.org:umu-208218DiVA, id: diva2:1756576
Funder
Swedish Cancer Society, CAN 2017/735Swedish Research Council, 2017-04028Swedish Research Council, 2021-05117Swedish Cancer Society, 20 1230 PjFUmeå UniversityAvailable from: 2023-05-12 Created: 2023-05-12 Last updated: 2023-09-05Bibliographically approved

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Larsson, ElinMorén, BjörnLundmark, Richard

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Larsson, ElinMorén, BjörnLundmark, Richard
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Department of Integrative Medical Biology (IMB)Umeå Centre for Microbial Research (UCMR)Molecular Infection Medicine Sweden (MIMS)
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Journal of Cell Biology
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